scholarly journals Leishmania infantum in the reproductive organs of dogs

2021 ◽  
Vol 51 (10) ◽  
Author(s):  
Diogo Tiago da Silva ◽  
Maria Luana Alves ◽  
Julio Cesar Pereira Spada ◽  
João Augusto Franco Leonel ◽  
Julia Cristina Benassi ◽  
...  

ABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. infantum in the reproductive organs of male and female dogs. Using histochemistry, immunohistochemistry, and PCR, we examined tissue samples from the reproductive organs of 8 male dogs and 16 female dogs diagnosed with leishmaniasis. Despite the absence of macroscopic or microscopic lesions in these organs, we observed L. infantum amastigotes in tissue samples from the testis and the uterus. PCR and sequencing of these tissues revealed sequences that matched 100% with L. infantum DNA available at GenBank. The presence of L. infantum amastigotes and DNA in testicular and uterine tissue samples suggested that these organs can harbor the parasite without associated macroscopic or microscopic lesions, and this can be especially important in the vertical and venereal transmission of leishmaniasis in dogs.

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jairo Alfonso Mendoza-Roldan ◽  
Maria Stefania Latrofa ◽  
Roberta Iatta ◽  
Ranju R. S. Manoj ◽  
Rossella Panarese ◽  
...  

Abstract Background Leishmania tarentolae is a protozoan isolated from geckoes (Tarentola annularis, Tarentola mauritanica), which is considered non-pathogenic and is transmitted by herpetophilic Sergentomyia spp. sand flies. This species occurs in sympatry with Leishmania infantum in areas where canine leishmaniasis is endemic. In the present study, we investigated the circulation of L. tarentolae and L. infantum in sand flies, dogs and lizards in a dog shelter in southern Italy, where canine leishmaniasis by L. infantum is endemic. Methods Sheltered dogs (n = 100) negative for Leishmania spp. (March 2020) were screened by immunofluorescence antibody test (IFAT) using promastigotes of both species at two time points (June 2020 and March 2021). Whole blood from dogs, tissues of Podarcis siculus lizards (n = 28) and sand flies (n = 2306) were also sampled and tested by a duplex real-time PCR (dqPCR). Host blood meal was assessed in sand flies by PCR. Results Overall, 16 dogs became positive for L. infantum and/or L. tarentolae by IFAT at one or both sampling periods. One canine blood sample was positive for L. infantum, whilst two for L. tarentolae by dqPCR. At the cytology of lizard blood, Leishmania spp. amastigote-like forms were detected in erythrocytes. Twenty-two tissue samples, mostly lung (21.4%), scored molecularly positive for L. tarentolae, corresponding to 10 lizards (i.e., 35.7%). Of the female Sergentomyia minuta sampled (n = 1252), 158 scored positive for L. tarentolae, four for L. infantum, and one co-infected. Two Phlebotomus perniciosus (out of 29 females) were positive for L. tarentolae. Engorged S. minuta (n = 10) fed on humans, and one P. perniciosus, positive for L. tarentolae, on lagomorphs. Conclusions Dogs and lacertid lizards (Podarcis siculus) were herein found for the first time infected by L. tarentolae. The detection of both L. tarentolae and L. infantum in S. minuta and P. perniciosus suggests their sympatric circulation, with a potential overlap in vertebrate hosts. The interactions between L. tarentolae and L. infantum should be further investigated in both vectors and vertebrate hosts to understand the potential implications for the diagnosis and control of canine leishmaniasis in endemic areas. Graphical abstract


Acta Tropica ◽  
2016 ◽  
Vol 161 ◽  
pp. 41-43 ◽  
Author(s):  
Viviana Pinedo-Cancino ◽  
Márcia Dalastra Laurenti ◽  
Norival Kesper ◽  
Eufrosina Setsu Umezawa

Acta Tropica ◽  
2015 ◽  
Vol 150 ◽  
pp. 218-223 ◽  
Author(s):  
Paola A. Barroso ◽  
M.Cecilia Nevot ◽  
Carlos L. Hoyos ◽  
Fabricio M. Locatelli ◽  
Juan J. Lauthier ◽  
...  

1859 ◽  
Vol 9 ◽  
pp. 600-601

The author briefly described the male and female reproductive organs of Comatula. When the ova are mature, and before impregnation, they are protruded and remain hanging from the ovarian orifice, entangled in the areolar tissue of the everted ovary. In this position impregnation appears usually to take place. After segmentation of the yelk, a solid nucleus is formed in the centre of the mulberry yelk-mass. This nucleus becomes invested in a special membrane, and into this embryonic mass the remainder of the yelk is gradually absorbed. Ciliary motion is observed at various points on the surface of the inclosed embryo, which finally assumes its characteristic form. The young larva, on escaping from the egg, consists of a homogeneous mass of pale-yellow granular matter, with scattered nuclei, cells, and oil-globules. It is barrel-shaped, and girded at intervals with about five broad ciliated bands.


Author(s):  
Dennis Harding

Contemporary studies commonly stress the belief that, even if sex is biologically determined, gender by contrast is a social and cultural construct (Sofaer and Sørensen, 2012). Even biological sex entails varying degrees of male and female attributes in terms of chromosomes and DNA if not in terms of reproductive organs, so that, contrary to the bipolar model of sex, contemporary studies of gender tend to think in terms of a spectrum that includes composite gender or a third gender that is neither male nor female in what Arnold (2006: 155) described as ‘a suprabinary gender system’. In the case of the Byzantine eunuchs or the Indian hijra cited by Croucher (2012: 174–5), these could be regarded as socially constructed, and it is not here suggested that such categories existed in Iron Age Britain or Europe. It is important, however, to be clear that conventional western sexual stereotypes and conceptions of gender roles in child-rearing, food production, and warfare, for example, need not have pertained in non-classical societies in antiquity. Gender issues in the study of funerary archaeology have gained a prominence in the last twenty years not simply as a result of theoretical considerations but also because of more intensive interest in osteological research, as a result of which there has been a greater recognition of the fact that identifying sex may involve evaluation of a spectrum of criteria rather than simple bipolar options. Though pelvic bones remain crucial to assessing sex, the skull and other major bones can also be indicative, and not infrequently the evidence remains equivocal, even where the skeleton is reasonably well preserved. Accordingly, some of the skeletons from the eastern Yorkshire cemeteries were deemed to show ‘contra’ indications, that is male and female characteristics in equal measure, in a gradation of assessment that also included ‘definite’, ‘probable’, and ‘possible’ identifications (Stead, 1991). Furthermore, though sex is biologically determined, osteology may be affected by cultural factors such as the degree of physical exercise that the individual habitually engages in, so that the criteria observed by the osteologist may suggest a physique normally associated with the opposite sex.


1988 ◽  
Vol 71 (2) ◽  
pp. 410-414 ◽  
Author(s):  
David T Williams ◽  
Guy L Lebel ◽  
Elizabeth Junkins

Abstract Human adipose tissue samples obtained during autopsies in 6 Canadian Great Lakes municipalities were analyzed for chlorobenzenes, polychlorobiphenyls, and organohalogen pesticide residues. The frequency of occurrence and the range and mean for 2JJ organohalogen residues are reported for male and female donors in each municipality. Overall mean residue values in females were significantly higher than those in males for hexachlorobenzen;, β-HCH, p,p'- DDE,»,/» -DDD + o,p'-DDT, and p,p'-DDT. The means and ranges of residue values were similar to those reported in previous Canadian surveys.


2003 ◽  
Vol 63 (4) ◽  
pp. 695-703 ◽  
Author(s):  
R. S. S. Santos ◽  
L. R. Redaelli ◽  
L. M. G. Diefenbach ◽  
H. P. Romanowski ◽  
H. F. Prando

The state of development of the internal reproductive organs of male and female Oebalus poecilus (Dallas) as well as the body fat amount in the abdominal cavity during hibernation, of individuals sampled in bamboo litter in Eldorado do Sul (30º02'S and 51°23'W), RS, Brazil was investigated. Females and males showed the abdominal cavity filled with body fat in the beginning of the hibernation phase. The decrease in fat reserve level occurred from August on for males and from October on for females. Ovaries and testis doubled in length and tripled in width from immature to the reproductive phase. Male sexual maturation occurred in the hibernation sites while for females it occurred later on outside of the sites. Reproductive organ immaturity and abdominal body fat hypertrophy characterized the diapause of O. poecilus.


2005 ◽  
Vol 17 (4) ◽  
pp. 393 ◽  
Author(s):  
C. Jackson ◽  
R. T. F. Bernard

The effects of winter food supplementation on reproduction in the seasonally breeding four-striped field mouse Rhabdomys pumilio were investigated at Mountain Zebra National Park in the Eastern Cape Province of South Africa. On both control and supplemented grids, reproductive activity in females was inhibited; no pregnant females were collected and juveniles were only present in the first winter month. The provision of additional food resulted in an increase in body mass and mass of the male and female reproductive organs. However, all males, from both grids, were spermatogenically active. Ovarian activity was not stimulated by the provision of additional food, but the development of the uterus was and the endometrium was thicker and more vascularised in mice from the supplemented grid than from the control grid. We conclude that seasonal reproduction in R. pumilio is controlled by the females, in which reproductive activity is inhibited in winter. However, the provision of supplementary food was not sufficient to override the reproductive inhibition.


1996 ◽  
Vol 76 (3) ◽  
pp. 463-464 ◽  
Author(s):  
W. A. Cerbito ◽  
M. P. B. Wijayagunawardane ◽  
M. Takagi ◽  
K. Sato ◽  
A. Miyamoto ◽  
...  

Bovine uterine horns with both ovaries containing a corpus luteum (CL) were compared for progesterone (P4) and oxytocin (OT) concentrations during the luteal phase of the estrous cycle. Uterine tissue samples from five Holstein cows with bilateral CL obtained from the slaughterhouse were used for this study. No significant difference was observed in P4 and OT levels in the right and left horns with corpora lutea in both ovaries. The data clearly indicate that both sides of the uterine horn having a functional CL are exposed to similar levels of P4 and OT, supporting the hypothesis that luteal products are delivered locally to the uterus. Key words: Progesterone, oxytocin, uterine horn, bilateral, corpus luteum, cow


Zootaxa ◽  
2019 ◽  
Vol 4674 (2) ◽  
pp. 283-295 ◽  
Author(s):  
ŁUKASZ PRZYBYŁOWICZ ◽  
VINCENT MAICHER ◽  
GYULA M. LÁSZLÓ ◽  
SZABOLCS SÁFIÁN ◽  
ROBERT TROPEK

Amerila is one of the most studied Afrotropical genera of Arctiinae. However, based on a regionally constrained sample of specimens from Mount Cameroon, we show how superficial our knowledge on these tiger moths is. Among six collected Amerila species, A. femina’s female is described here for the first time, and A. mulleri and A. roseomarginata had never been recorded before in the country. Moreover, novel biological data are presented, including individual species’ elevational ranges. Finally, female reproductive organs of the genus are illustrated here for the first time. The value of such regional studies is highlighted, with some remarks on necessary requirements of such small-scaled field sampling. 


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