scholarly journals Assessment of heavy metals and its impact on DNA fragmentation in different fish species

2020 ◽  
Vol 80 (4) ◽  
pp. 823-828
Author(s):  
S. Sultana ◽  
F. Jabeen ◽  
T. Sultana ◽  
K. A. AL-Ghanim ◽  
F. Al-Misned ◽  
...  

Abstract This study was conducted to assess water pollution by examining DNA fragmentation in selected fish organs (kidney, liver, gills, and muscle tissue) from Wallago attu, Sperata sarwari, Vulgaris vulgaris, and Labeo rohita collected from a known polluted section of the Chenab River, Pakistan, and from a control site. The fish were caught using a gill net and were assigned to three different weight groups (W1, W2, and W3) to study the degree of variation in DNA fragmentation in relation to body weight. In fish from the polluted site, DNA fragmentation was higher in kidney, liver, gills, and muscles, compared to the control. No significant DNA fragmentation was observed in fish collected from the control site. Highly significant (P < 0.01) relationship between body weight and DNA fragmentation was found in the organs of fish procured at the contaminated site. DNA fragmentation in body organs was found to be affected by the concentrations of lead, copper, nickel, and cadmium in W. attu, S. sarwari, L. rohita, and V. vulgarus harvested from Chenab River. DNA fragmentation in different freshwater fish species is therefore a reliable biomarker of water pollution.

2008 ◽  
Vol 38 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Wanderley Rodrigues Bastos ◽  
Mauro de Freitas Rebelo ◽  
Márlon de Freitas Fonseca ◽  
Ronaldo de Almeida ◽  
Olaf Malm

Over the last 20 years several projects carried on the Madeira River basin in the Amazon produced a great amount data on total Hg concentration in different fish species. In this paper we discuss temporal trends in Hg contamination and its relation to body weight in some of those fishes, showing that even within similar groups, such as carnivorous and non-migratory fish, the interspecies variability in Hg accumulation is considerable.


2016 ◽  
Vol 14 (2) ◽  
Author(s):  
Fernanda De Mello ◽  
Daruzi Felippe ◽  
Leandro C. Godoy ◽  
Nívia Lothhammer ◽  
Luis R. J. Guerreiro ◽  
...  

ABSTRACT This study aimed to evaluate muscle organization in tambaqui in order to describe the muscle growth process. We analyzed the morphometric pattern of fibers from white muscle of young-adults (300 days) by smaller diameter. The organization of white muscle exhibited a typical morphological pattern found in other fish species. Heavier animals showed higher frequency of larger diameter fibers (>50 μm ) and smaller animals had higher frequency of smaller diameter fibers (<20 μm ) (P =0.005). However, both animals showed the same frequency of intermediate diameter fibers (20-50 μm ). Body weight showed a positive correlation with muscle diameter fiber (r=0.45), being 20-50 μm the diameters that contributed the most to animal weight (P <0.0001). A weak correlation between fiber diameter and animal sex was observed (r=0.2). Females showed higher frequency of large fiber diameters (>50 μm ) than males. However, there was no difference between body weight and sex (P =0.8). Our results suggest that muscle growth is by hypertrophy and hyperplasia due to a mosaic appearance from different diameters fibers, which is characteristic of large size fish species.


2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Kristoforus Slamet Suryadi Junardi, Tri Rima Setyawati

Diversity of fish species is important  known to determinate river. The purpose of this study was to determine the diversity and distribution of fish also environmental conditions in the lower reaches of the Sekadau River, West Kalimantan. Data collection is conducted from March to May 2018 using posat and gill net. The results showed that the most common Cyprinidae family was found in Rasbora argyrotaenia  and Osteochilus microcephalus with dominance value  0.72 and 0.70 respectively.


2010 ◽  
Vol 29 (2_suppl) ◽  
pp. 3S-14S ◽  
Author(s):  
Bruce K. Bernard ◽  
Kazuyuki Ubukata ◽  
Ryuichi Mihara ◽  
Yoshiaki Sato ◽  
Hiroyuki Nemoto

Pharmacokinetics of a single gavage dose of 14C-labeled dihydrocapsiate (10 mg/kg) were investigated in male rats. Maximal plasma concentration was achieved in 40 minutes and exhibited an apparent half-life of 2.4 hours. Excretion of radioactivity in the urine, feces, and expired air was 78.2%, 19.4%, and 0.5% of the dose, respectively. Highest tissue concentrations were achieved in the kidney, liver, and blood; the data indicate that radioactivity accumulation following daily exposure at a dose of 10 mg/kg body weight is unlikely. Radioactivity in the plasma was associated with metabolites and their conjugates, probably vanillyl alcohol, vanillic acid, glucuronide of vanillyl alcohol, sulphate of vanillyl alcohol, and sulphate of vanillic acid. These results suggest dihydrocapsiate is metabolized by hydrolysis in the gut, or esterase or other enzymes in the blood, and the metabolites were rapidly absorbed and converted to their conjugates in the liver and eliminated by the kidneys into the urine.


2020 ◽  
Vol 65 (10) ◽  
pp. 154-163
Author(s):  
Hung Nguyen Phuc ◽  
Anh Nguyen Phuong

Fermentation has been suggested as an economical and effective method to eliminate anti-nutritional factors and improve the nutritional value of Defatted soybean meal (SBM). The present study aimed to examine the effects of Aspergillus oryzae fermented SBM (FSBM) on plasma cholesterol and bile acid levels in hybrid tilapia and pompano. Two isonitrogenous and isoenergetic experimental diets for each fish species were formulated with SBM and FSBM as main ingredients. The diets were denoted as SBMD (SBM-based diet) and FSBMD (FSBM-based diet). Thirty juvenile hybrid tilapia (initial body weight 55 g) and eighteen juvenile pompanos (initial body weight 61 g) were allocated to each of the 4 concrete tanks (1500-L holding capacity) and each of the 4 circular polyvinyl chloride tanks (500-L holding capacity), respectively, resulting in two replicate tanks per dietary treatment for each fish species. For 4 weeks, the fish were handfed the experimental diets to apparent satiation twice daily. The results showed that feeding FSBMD tended to increase plasma total cholesterol level, gallbladdersomatic index, anterior intestinal total bile acid level, and posterior\anterior intestinal total bile acid reduction rate compared to SBMD in both hybrid tilapia and pompano. The differences in these parameters between FSBMD and SBMD dietary groups were significant in hybrid tilapia (P < 0.05), while no significant differences were found between pompano fed FSBMD and SBMD. These results indicated that fermentation of SBM with A. oryzae could effectively improve physiological conditions of hybrid tilapia fed soybean proteinbased diet and suggested that inclusion of A. oryzae FSBM might enhance growth and feed performances of the fish.


2018 ◽  
Vol 53 (2) ◽  
pp. 155-160
Author(s):  
MR Islam ◽  
MG Azom

For evaluation of Fulton’s condition factor (CFf), relative body weight (BWr) and form (a3.0) factor affecting growth, 5 fishes (Aspidoparia jaya, Gagata cenia, G. youssoufi, Salmophasia bacaila and S. phulo) were collected from the Atari River in Dinajpur district of Bangladesh. Significant differences (p < 0.05) were recorded among the values of CFf (0.47-1.56) but not for BWr (79.60- 128.39). Based on CFf values, analysis of similarities (ANOSIM, p < 0.01) revealed that maximum distances (R = 0.9946) were found between G. youssoufi and S. bacaila while lowest (R = 0.2175) between G. cenia and S. phulo. Using two-dimensional nMDS scaling of CFf and BWr, G. youssoufi showed good health condition (CFf = 1.22±0.01; BWr = 100.63±0.79) and were separated (stress = 0.0003) from other fishes. Lowest a3.0 value was noted for S. bacaila (0.004) and highest for G. youssoufi (0.012). Therefore, G. youssoufi showed healthier and former body with more adaptability in this river than those of others. These are the first records for proper management of above mentioned fish species and future research in the near future.Bangladesh J. Sci. Ind. Res.53(2), 155-160, 2018


2012 ◽  
Vol 4 (8) ◽  
Author(s):  
Abdulmojeed Yakubu ◽  
Samuel A. Okunsebor ◽  
Alex A. Kigbu ◽  
Akeem O. Sotolu ◽  
Terzungwe D. Imgbian

Author(s):  
Vu Ngoc Ut ◽  
Au Van Hoa ◽  
Huynh Phuoc Vinh

Fish biodiversity on Hau (Bassac) river was investigated to assess the status of species composition and fishing by fishing gears during a year. Sampling was implemented monthly at the upper part (An Giang province), middle part (Can Tho City) and lower part (Soc Trang province) of Hau River using trawl net as main sampling gear. Additionally, fish composition was also recorded from four other most popular fishing gears including cast net, gill net, fixed net and hook operated in the study sites. Fish species composition was determined by fishing gears and their abundance (CPUE) was calculated only from the main sampling gear (trawl net). The results showed that a total of 176 fish species belonging to 16 orders and 49 families was recorded. Perciformes was the most abundant group with 51 species followed by Cypriniformes with 46 species. The number of fish species was decreasing from upper part to lower part. Trawl net was considered the most destructive gear as up to 145 fish species caught by this device, followed by gill net with 98 species, fixed net 75, cast net 57, and hooks 16 species. CPUE was very low ranging from  0.53 kg.ha−1 h−1 to 26.30 kg.ha−1 h−1. Higher CPUE was recorded at lower part in compared to upper part and middle part, and at dry season in compared to rainy season. Regulation on fishing gears, fishing ground and season should be taken into consideration to protect and conserve the resources.


1982 ◽  
Vol 65 (4) ◽  
pp. 884-887 ◽  
Author(s):  
Mary W Trucksess ◽  
Leonard Stoloff ◽  
William C Brumley ◽  
David M Wilson ◽  
Otha M Hale ◽  
...  

Abstract Aflatoxicol (AFL) and aflatoxins B1 and M1 were found in tissues (kidney, liver, and muscle) of feeder pigs given an estimated LD50 oral dose of B1 (1.0 mg/kg body weight) provided as a rice culture of Aspergillus flavus and of market-weight pigs fed a naturally contaminated feed, containing aflatoxin Bi at a level of 400 ng/g from corn, for 14 days. The residues in all tissues decreased with time after treatment in both groups, with no detectable residues (approximate detection limits, ng/g, B1 0.03, M1 0.05, AFL 0.01) in pig tissues from the feeding experiment 24 h after withdrawal of aflatoxin-contaminated feed. B1 and M1, when found in the feeding experiment, were at about the same levels in all tissues except the kidney, in which M1 was the dominant aflatoxin. The level of AFL, when detected, was about 10% of the B1 level.


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