scholarly journals PCR-RFLP Based genetic diversity of Plasmodium vivax genotypes in district Mardan, Pakistan

2022 ◽  
Vol 82 ◽  
Author(s):  
I. Ullah ◽  
S. G. Afridi ◽  
A. U. Khan ◽  
M. Israr ◽  
A. Ali ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Vivax ◽  
Nested Pcr ◽  
Vaccine Development ◽  
Restriction Enzymes ◽  
Type A ◽  
P Value ◽  
Type B ◽  
Pcr Rflp ◽  
Type C

Abstract Plasmodium vivax is the most common human malaria parasite in Asian countries including Pakistan. Present study was designed to explore the genetic diversity of plasmodium vivax genotypes based on Pvmsp-3α and Pvmsp-3βgenes using allelic specific nested PCR and RFLP assays markers from field isolates in district Mardan, Pakistan. Blood samples of 200 P. vivax malarial patients were collected after taking their written informed consent. Genetic diversity in nested PCR products was determined by Restriction Fragment Length Polymorphism (RFLP) utilizing Alu1 and PstI restriction enzymes for alpha and beta gene products digestion, respectively. For analysis the genetic diversity of the sub allelic variants of Pvmsp3α and Pvmsp3β genes, Chi-Square test was performed by utilizing Minitab programming software 18. The P value 0.05 was considered as statistically significant. For Pvmsp-3α genes after gel electrophoresis of digested products, four distinct genotypes were obtained from total of 50 samples; type A: 35 (70%) (1.5-2.0 kb), 12 of type B (24%) (1.5-1.7 kb), 2 of type C (4%) (0.5-1.5) and one for type D (2%) (0.5-0.65 kb) which could be characterized into 9 allelic pattern (A1-A4, B1-B3, C1, D), in which A3 remained the most predominant. For Pvmsp-3βgenes, three distinct genotypes were obtained from 50 samples; 40(80%) of type A (1.5-2.5 kb), 9 (18%) of type B (1.0-1.5kb) and 1(2%) of type C (0.65 kb) which could be characterized into 6 allelic patterns (A1-A3, B1-B2, and C1). Most dominant one in Type A was A1 alleles which were noted (46%), while in Type B, the most dominant were B1 (10%).This study is the first ever report of molecular epidemiology and genetic variation in Pvmsp-3α and Pvmsp-3β genes of P. vivax isolates by using PCR/RFLP from District Mardan and showed a remarkable level of genetic diversity in the studied genes of circulating parasites in the study area. The results of this study will contribute in future studies about the genetic structure of parasite and vaccine development against the malaria.

scholarly journals THE USE OF ARCHIVED GIEMSA-STAINED BLOOD SMEARS AND RDT FOR PCR-BASED GENOTYPING OF Plasmodium vivax MEROZOITE SURFACE PROTEIN-1 IN CENTRAL KALIMANTAN PROVINCE, INDONESIA: PCR-Based Genotyping of Pvmsp-1 in Central Kalimantan Province, Indonesia

2021 ◽  
Vol 16 (1) ◽  
pp. 13-20
Author(s):  
Trilianty Lestarisa ◽  
Heny Arwati ◽  
Yoes Prijatna Dachlan ◽  
Soedjajadi Keman ◽  
Din Syafruddin
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Vivax ◽  
Allelic Variation ◽  
Control Program ◽  
Type A ◽  
Merozoite Surface Protein ◽  
Single Step ◽  
Type B ◽  
Allelic Variants ◽  
Central Kalimantan

Background: Plasmodium vivax is transmitted most across the country of Indonesia. The country has set out a malaria elimination program by 2030. The information on genetic diversity of malarial parasites relates to malaria transmission in an endemic area may provide the information that can help the malaria control program to achieve the target. Therefore, the purpose of this study was to determine the genetic diversity of the Pvmsp-1 gene in Central Kalimantan Province. Materials and Methods: Samples were 140 of archived Giemsa-stained blood smear and rapid detection test. Samples were divided into the indigenous and migrant populations. After confirmation by single-step PCR, only P. vivax and mixed infection samples were amplified to nested PCR for genotyping of Pvmsp-1 allelic variation in segments F1, F2, and F3. Results: Genotyping of 23 PCR positive samples resulted in 13 genotypes. In segment F1, three allelic variants type A containing subtype A1 (1,050 bp), A2 (350 bp), A3 (150 bp), and type B (100 bp). In segment F2, mono genotypes were detected as variant type A (1,050 bp) and type B3 (150 bp), multiple genotypes were detected as type B containing subtype B1 (250 bp), B2 (200 bp), and B3 (150bp). In segment F3, three allelic variants generated from four mono genotypes were type A (350 bp), type B (300 bp), and two type C (250 bp). Conclusion: The low allelic variation of Pvmsp-1 gene may reflect the actual situation of the low malaria endemic status of the study sites.

scholarly journals The effectiveness of TiO2 additions to mortar to maintain initial conditions in terms of its reflectance to solar radiation

2017 ◽  
Vol 17 (3) ◽  
pp. 39-56 ◽  
Author(s):  
Sérgio Roberto Andrade Dantas ◽  
Fulvio Vittorino ◽  
Kai Loh
Keyword(s):  
Solar Radiation ◽  
Ultraviolet Radiation ◽  
Initial Conditions ◽  
Type A ◽  
Just Noticeable Difference ◽  
Type B ◽  
Climate Conditions ◽  
Type D ◽  
Type C ◽  
Over Time

Abstract Contact of facades with degradation agents and direct incidence of ultraviolet radiation on external coatings make them more opaque over time, affecting their colour and reflectance characteristics. This study evaluated the effect of adding different TiO2 contents to mortars applied in concrete substrates in order to verify the reflectance maintenance on surfaces after exposure over time. Mortar with different concentrations of TiO2 (1%, 5%, 10%) were produced in relation to the total dry premix, added as a powder and compared to unpainted mortar without TiO2 (type "A") and painted mortar without TiO2 (type "B"), both used as a reference for colour and reflectance. Exposed over 16 months to climate conditions in São Paulo, regarding the maintenance of reflectance and solar radiation, the results showed that type "B" (0%TiO2) painted mortar presented the best performance. Type "C" (1%TiO2) and type "D" (5%TiO2) unpainted mortar remained more stable. Type "A" (0%TiO2) and type "E" (10%TiO2) unpainted mortar showed greater differences according to the Just Noticeable Difference (JND) range caused by dirt pick up.

Judgments of Grammatically by Normal and Language-Disordered Children

1977 ◽  
Vol 42 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Betty Z. Liles ◽  
Martin D. Shulman ◽  
Susan Bartlett
Keyword(s):  
Word Order ◽  
Sentence Type ◽  
Type A ◽  
Order Type ◽  
Specific Reference ◽  
Type B ◽  
Normal Children ◽  
Grammatical Errors ◽  
Type C

Fifteen linguistically normal children and 15 linguistically deviant children were presented with three types of agrammatical sentences. The subjects were asked to judge the sentences as right or wrong and to change the sentences judged as wrong, rendering them correct. The three types of agrammatical sentences represented rule violations of syntactic agreement (Type A), lexical restrictions (Type B), and word order (Type C). The two groups of children were compared in terms of the number of sentences of each type that were recognized as agrammatical. Those productions which represented the child’s correction of agrammatical sentences were subjected to descriptive analyses (percentages) with specific reference to the number of attempted changes and the number of those changes which demonstrated corrections of the specific deviation from well formedness. Results indicated that the two groups of subjects were significantly different in their ability to recognize grammatical errors in sentence Types A and C, but did not differ in their ability to recognize errors in sentence Type B. The descriptive comparison of the groups' verbal corrections reflected this trend, in that the language-disordered subjects made corrections specific to the error on more of the Type B sentences (for example, “The dog writes the food.”) than on Types A (for example, “'She will pick some flowers last week.”) or C (for example, “Get and come your dinner.”). Linguistically normal children accurately corrected 90.7% of the sentences judged as agrammatical; this percentage did not vary more than 1% across sentence types.

scholarly journals Structural and fractal characterization of adsorption pores of middle–high rank coal reservoirs in western Yunnan and eastern Guizhou: An experimental study of coals from the Panguan syncline and Laochang anticline

2018 ◽  
Vol 37 (1) ◽  
pp. 251-272 ◽  
Author(s):  
Junjian Zhang ◽  
Chongtao Wei ◽  
Gaoyuan Yan ◽  
Guanwen Lu
Keyword(s):  
Specific Surface ◽  
Surface Area ◽  
Specific Surface Area ◽  
Pore Volume ◽  
Pore Diameter ◽  
Structural Parameters ◽  
Total Pore Volume ◽  
Type A ◽  
Type B ◽  
Type C

To better understand the structural characteristic of adsorption pores (pore diameter < 100 nm) of coal reservoirs around the coalbed methane production areas of western Yunnan and eastern Guizhou, we analyzed the structural and fractal characteristics of pore size range of 0.40–2.0 nm and 2–100 nm in middle–high rank coals ( Ro,max = 0.93–3.20%) by combining low-temperature N2/CO2 adsorption tests and surface/volume fractal theory. The results show that the coal reservoirs can be divided into three categories: type A ( Ro,max < 2.15%), type B (2.15% <  Ro,max <2.50%), and type C ( Ro,max > 2.15%). The structural parameters of pores in the range from 2 to 100 nm are influenced by the degree of coal metamorphism and the compositional parameters (e.g., ash and volatile matter). The dominant diameters of the specific surface areas are 10–50 nm, 2–50 nm, and 2–10 nm, respectively. The pores in the range from <2 nm provide the largest proportion of total specific surface area (97.22%–99.96%) of the coal reservoir, and the CO2-specific surface area and CO2-total pore volume relationships show a positive linear correlation. The metamorphic degree has a much greater control on the pores (pore diameter less than 2 nm) structural parameters than those of the pore diameter ranges from 2 to 100 nm. Dv1 and Dv2 can characterize the structure of 2–100 nm adsorption pores, and Dv1 (volume heterogeneity) has a positive correlation with the pore structural parameters such as N2-specific surface area and N2-total pore volume. This parameter can be used to characterize volume heterogeneity of 2–10 nm pores. Dv2 (surface heterogeneity) showed type A > type B > type C and was mainly affected by the metamorphism degree. Ds2 can be used to characterize the pore surface heterogeneity of micropores in the range of 0.62–1.50 nm. This parameter has a good correlation with the pore parameters (CO2-total pore volume, CO2-specific surface area, and average pore size) and is expressed as type C < type B < type A. In conclusion, the heterogeneity of the micropores is less than that of the meso- and macropores (2–100 nm). Dv1, Dv2, and Ds2 can be used as effective parameters to characterize the pore structure of adsorption pores. This result can provide a theoretical basis for studying the pore structure compatibility of coal reservoirs in the region.

scholarly journals Molecular Characterization ofCryptosporidiumspp. in Wild Rodents of Southwestern Iran Using 18s rRNA Gene Nested-PCR-RFLP and Sequencing Techniques

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Jasem Saki ◽  
Masoud Foroutan-Rad ◽  
Reza Asadpouri
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
18S Rrna ◽  
Restriction Enzymes ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Wild Rodents ◽  
Nested Polymerase Chain Reaction ◽  
Pcr Rflp ◽  
Southwest Of Iran

Background. Rodents could act as reservoir forCryptosporidiumspp. speciallyC. parvum, a zoonotic agent responsible for human infections. Since there is no information aboutCryptosporidiuminfection in rodents of Ahvaz city, southwest of Iran, hence, this survey was performed to determine the prevalence and molecular characterization ofCryptosporidiumspp. in this region.Materials and Methods. One hundred rodents were trapped from different regions of Ahvaz city. Intestine contents and fecal specimens of rodents were studied using both microscopy examination to identify oocyst and nested-polymerase chain reaction (PCR) technique for 18s rRNA gene detection. Eventually restriction fragment length polymorphism (RFLP) method usingSspIandVspIrestriction enzymes was carried out to genotype the species and then obtained results were sequenced.Results. Three out of 100 samples were diagnosed as positive and overall prevalence ofCryptosporidiumspp. was 3% using both modified Ziehl-Neelsen staining under light microscope and nested-PCR (830 bp) methods. Afterwards, PCR-RFLP was performed on positive samples andC. parvumpattern was identified. Finally PCR-RFLP findings were sequenced and presence ofC. parvumwas confirmed again.Conclusions. Our study showed rodents could be potential reservoir forC. parvum. So an integrated program for control and combat with them should be adopted and continued.

scholarly journals Housing Condition of Coastal Area in Bangladesh: A Case Study of Kutubdia, Cox’s Bazaar

2015 ◽  
Vol 6 (1) ◽  
pp. 15-20 ◽  
Author(s):  
M Mohiuddin ◽  
MB Latif
Keyword(s):  
Type A ◽  
P Value ◽  
Type B ◽  
Laboratory Examination ◽  
Chi Square ◽  
E Coli ◽  
Significant Difference ◽  
Different Types ◽  
Hygienic Measures

This study was conducted to determine the contamination of E. coli and Salmonella for different types of foods in Chittagong city area. In case of laboratory examination, almost half (49.58%) of the samples were contaminated where 28.75% positive for E. coli. and 20.83% for Salmonella. The positive cases for type A sample was 20 out of 96 constituting 20.83% whereas the positive cases for type B sample was 49 out of 144 constituting 34.03% for E. coli. The positive cases of type A sample was 15 out of 96 making up 15.63% and for type B sample was 35 out of 144 making up 24.31% for Salmonella.A chi-square (?2) test was used to examine the equality of observed proportions for E. coli and Salmonella of each item of both types of food where significant difference among the observed proportion for E. coli (p-value<0.01) and Salmonella (p-value=0.032) for different items of A type and for E. coli (p-value<0.01) for different items of B type were observed and an odds ratio (OR) was measured for association between exposure and outcome where the probability of contaminated by E. coli of type B food is higher than contaminated of type A food (OR= 1.96 and CI:1.07-3.58). The probability of contaminated by Salmonella of type B food was higher than type A food (OR= 1.73 and CI: 0.89-3.39).The comparatively high bacteria in type B samples indicated contamination from water, practice of inadequate hygienic measures, mishandling, improper storage, inadequate cooking and above all unhygienic condition of the retail shops.DOI: http://dx.doi.org/10.3329/jesnr.v6i1.22034 J. Environ. Sci. & Natural Resources, 6(1): 15-20 2013

scholarly journals Postsurgical residual lung complications following left upper trisegmentectomy

2019 ◽  
Author(s):  
Yutaro Koike ◽  
Aritoshi Hattori ◽  
Takeshi Matsunaga ◽  
Kazuya Takamochi ◽  
Shiaki Oh ◽  
...  
Keyword(s):  
Surgical Intervention ◽  
Type A ◽  
X Rays ◽  
Limited Information ◽  
Type B ◽  
Type D ◽  
Thoracic Computed Tomography ◽  
Isolated Segment ◽  
Type C ◽  
Lung Torsion

AbstractOBJECTIVESSegmentectomy has become an increasingly popular surgical procedure for small-sized lung lesions. Left upper trisegmentectomy (LUTS) is one of the most common segmentectomies performed because of its relative ease and simplicity; however, limited information is currently available on the specific postoperative complications associated with this procedure.METHODSAmong 2060 surgically resected cases in our institute between 2009 and 2016, 129 (6.2%) underwent LUTS. Postoperative chest X-rays and/or thoracic computed tomography (CT) scans were retrospectively assessed for all cases to assess postsurgical residual lung complications following LUTS. We categorized cases into 4 groups: type A (atelectasis of the lingular segment), type B (lung torsion of the lingular segment), type C (necrosis of the ‘isolated segment’) and type D (haematoma along stapling lines).RESULTSPostsurgical lung complications following LUTS were observed in 17 (13.1%) patients (type A: n = 7, type B: n = 1, type C: n = 4 and type D: n = 5). Three patients (2.3%) required surgical intervention because of type B (n = 1) and type C (n = 2), namely, decreased permeability and remaining ground glass opacities in the residual lung, showing an exacerbated systemic inflammatory response. In contrast, type A and D cases were successfully observed by chest CT without any surgical intervention, and patients recovered within a few months of surgery.CONCLUSIONSWe identified several postoperative residual lung complications following LUTS. Lung torsion or necrosis of the residual segment may require intensive care, including reoperation. Potentially serious complications always need to be ruled out after LUTS when radiological consolidation is detected postoperatively.

Research on Flow Units of Extra-Low Permeability Reservoirs in Fault Block Sheng554 of Shengping Oilfield

2013 ◽  
Vol 295-298 ◽  
pp. 3162-3165
Author(s):  
Lu Lu Zhou ◽  
Zi Nan Li ◽  
Jun Gang Liu ◽  
Yan Yun Zhang ◽  
Guang Qiang Shu
Keyword(s):  
Fluid Flow ◽  
Water Injection ◽  
Type A ◽  
Low Permeability ◽  
Type B ◽  
Flow Units ◽  
Fault Block ◽  
Remaining Oil ◽  
Type C ◽  
Low Permeability Reservoirs

Taking the example of the fourth member of the Lower Cretaceous Quantou formation reservoirs in fault block Sheng554 of Sanzhao sag, this article discusses the methodology of flow units in extra-low permeability reservoirs. The research on flow units in such reservoirs can be divided into two ranks, one is to determine the distribution of seepage barriers and inner connected sands， the other is to analyze the differentia of fluid flow in the inner connected sands so as to subdivide the flow units. The result shows that the pelitic barriers are rather developed in fault block Sheng554. Through the analysis of differentia of fluid flow, according to the value of flow zone index (FZI), the inner connected sands can be classified into three types of flow units, among which type A with FZI value greater than 1.0 has better permeable property and higher intensity of water injection, and the ability of permeability and water injection of type B with FZI value between 0.5 and 1.0 takes the second place, and type C is the worst flow unit with the worst permeable property and intensity of water injection with FZI value less than 0.5. Among the three types of flow units, type A poorly develops, while type B and type C develops well. The research on flow units can provide reliable geologic bases for forecasting the distribution of remaining oil in extra-low permeability reservoirs and for developing remaining oil in the study area.

A New Classification of Cochleovestibular Malformations and Implications for Predicting Speech Perception Ability after Cochlear Implantation

2015 ◽  
Vol 20 (2) ◽  
pp. 90-101 ◽  
Author(s):  
Sung-Wook Jeong ◽  
Lee-Suk Kim
Keyword(s):  
Speech Perception ◽  
Cochlear Implantation ◽  
Test Scores ◽  
Type A ◽  
Cochlear Nerve ◽  
Type B ◽  
New Classification ◽  
Type D ◽  
Type C

Objectives: The aims of this study were to introduce a new classification of cochleovestibular malformation (CVM) and to investigate how well this classification can predict speech perception ability after cochlear implantation in children with CVM. Methods: Fifty-nine children with CVM who had used a cochlear implant for more than 3 years were included. CVM was classified into 4 subtypes based on the morphology of the cochlea and the modiolus on temporal bone computed tomography (TBCT): normal cochlea and normal modiolus (type A, n = 16), malformed cochlea and partial modiolus (type B, n = 31), malformed cochlea and no modiolus (type C, n = 6), and no cochlea and no modiolus (type D, n = 6). Speech perception test scores were compared between the subtypes of CVM using analysis of covariance with post hoc Bonferroni test. Univariate and multivariate regression analyses were used to identify the significant predictors of the speech perception test scores. Results: The speech perception test scores after implantation were significantly better in children with CVM type A or type B than in children with CVM type C or type D. The test scores did not differ significantly between the implanted children with CVM type A or type B and those without CVM. In univariate regression analysis, the type of CVM was a significant predictor of the speech perception test scores in implanted children with CVM. Multivariate regression analysis revealed that the age at cochlear implantation, cochlear nerve size and preimplantation speech perception test scores were significant predictors of the postimplantation speech perception test scores. The chance of cochlear nerve deficiency increased progressively from CVM type A to type D. Conclusion: The new classification of CVM based on the morphology of the cochlea and the modiolus is simple and easy to use, and correlated well with postimplantation speech perception ability and cochlear nerve status. This simple classification of CVM using TBCT with cochlear nerve assessment by magnetic resonance imaging is helpful in the preoperative evaluation of children with CVM.

GROUPING OF ISOLATES OF A LOW-TEMPERATURE BASIDIOMYCETE ON THE BASIS OF CULTURAL BEHAVIOR AND PATHOGENICITY

1961 ◽  
Vol 39 (2) ◽  
pp. 297-306 ◽  
Author(s):  
E. W. B. Ward ◽  
J. B. Lebeau ◽  
M. W. Cormack
Keyword(s):  
Low Temperature ◽  
Host Plants ◽  
Type A ◽  
Effect Of Temperature ◽  
Western Canada ◽  
Type B ◽  
Highly Pathogenic ◽  
Cultural Behavior ◽  
Type C ◽  
Ph Range

Isolates of an unidentified low-temperature basidiomycete, associated with snow mold in Western Canada, were divided into three types, A, B, and C, on the basis of their general cultural appearance. Support for this classification was obtained when representative isolates of each type were examined to determine: the effect of temperature and pH on growth; tolerance of antibiotics and HCN; ability to liberate HCN in culture and in the host plant; pathogenicity.Type A isolates grew slowly under most conditions and were least tolerant of the extremes of temperature and pH employed, moderately inhibited by antibiotics, and strongly inhibited by HCN. They produced HCN in large quantities in host plants and none in culture. They were moderately pathogenic on grass and highly pathogenic on alfalfa. Type B isolates grew somewhat more rapidly than type A, especially at the upper and lower temperatures, and grew over a wider pH range. These isolates were more tolerant of antibiotics and HCN. They produced smaller quantities of HCN than type A in infected alfalfa plants but released large amounts in culture. They were less pathogenic than type A on alfalfa but similarly pathogenic on grass. Type C isolates were fast-growing forms which were strongly inhibited by antibiotics and HCN. They did not liberate HCN under any conditions and were not pathogenic.

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