scholarly journals Growth and antioxidant response of juvenile oysters Crassostrea sikamea and Crassostrea corteziensis treated with Streptomyces strains

2019 ◽  
Vol 71 (6) ◽  
pp. 1993-1998
Author(s):  
M. García-Bernal ◽  
R. Medina-Marrero ◽  
Á.I. Campa-Córdova ◽  
J.M. Mazón-Suástegui
Keyword(s):  
Weight Gain ◽  
Marine Invertebrates ◽  
Antioxidant Response ◽  
Treated Group ◽  
Control Group ◽  
Sod Activity ◽  
Probiotic Potential ◽  
Crassostrea Sikamea ◽  
Crassostrea Corteziensis ◽  
Significant Size

ABSTRACT The effect of three Streptomyces strains (N7, RL8 and V4) and a mixture of Bacillus (BMix) on the growth (Weight, Size) and superoxide dismutase activity (SOD) in hatchery-reared juvenile oysters Crassostrea corteziensis and Crassostrea sikamea was investigated to determine their probiotic potential. Microorganisms were added to culture water at 1×106 CFU/ml once a day during 30 days and all oysters fed daily a microalgae mix. Juveniles of C. sikamea treated with strains N7, RL8 and V4 had a significant weight gain compared to the control group. C. corteziensis juveniles treated with strains RL8 and BMix showed a significantly higher weight gain than the control group. No significant size increase was observed in any treated group for both oyster species. SOD activity significantly increased in C. sikamea treated with RL8 and with RL8, N7 and BMix in C. corteziensis. Streptomyces strains RL8 and N7 emerge as promising probiotic agents to cultivate C. sikamea and C. corteziensis and may also be useful to other molluscs and marine invertebrates .

Immune and Antioxidant Enzyme Response of Longfin Yellowtail (Seriola rivoliana) Juveniles to Ultra-diluted Substances Derived from Phosphorus, Silica and Pathogenic Vibrio

Homeopathy ◽  
2018 ◽  
Vol 108 (01) ◽  
pp. 043-053 ◽  
Author(s):  
José Mazón-Suástegui ◽  
Joan Salas-Leiva ◽  
Andressa Teles ◽  
Dariel Tovar-Ramírez
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Antioxidant Response ◽  
Culture Conditions ◽  
Control Group ◽  
Sod Activity ◽  
Over Expression ◽  
Pathogenic Vibrio ◽  
Low Concentrations ◽  
Mother Tincture

Background This research aimed to observe the effect of homeopathic treatments prepared from Vibrio parahaemolyticus and V. alginolyticus (H1) and commercial homeopathic medication Phosphoricum acidum and Silicea terra (H2) on the immune and antioxidant response in Seriola rivoliana juveniles under usual culture conditions and challenged with V. parahaemolyticus. Materials and Methods Quantitative polymerase chain reaction analysis was used to study changes in the expression of key genes related to immune response, cytokines (interleukin-1β [IL-1β]), adapter protein for cytokine release (MyD88) and piscidin and spectrophotometric techniques to analyze the activity of antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymes in Seriola rivoliana juveniles at 30 (weaning stage [WS]) and 60 (early juveniles [EJ]) days post-hatching. Results The H1 treatment led to over-expression of the IL-1β and MyD88 genes in fish at WS and EJ with respect to control, contrary to the H2 treatment that led to under-expression of the IL-1β, MyD88 and piscidin genes at the EJ stage. In fish challenged with V. parahaemolyticus, both H1 and H2 led to over-expression of IL-1β and MyD88; H2 caused an over-expression of piscidin. The SOD activity was higher in H1 with respect to H2 and the control group. CAT remained relatively stable with both H1 and H2 treatments. Conclusions The results suggest that the overall effect of H1 was due to the presence of unknown antigens in low concentrations, while the response to H2—specifically during challenge—may have been due to a stimulating effect of nano-structures, prevailing from mother tincture after sequential dilution/succussion, in a pathway similar to that attributed to nano-vaccines.

scholarly journals Effect of piglet castration with nonsurgical sterilant on the zootechnical performance and pork carcass quality

2016 ◽  
Vol 68 (6) ◽  
pp. 1487-1496 ◽  
Author(s):  
D.S. Lucas ◽  
E.T.F. Siqueira ◽  
M.M.H. Haguiwara ◽  
S.S. Azevedo ◽  
S.E. Yotsuyanagi ◽  
...  
Keyword(s):  
Weight Gain ◽  
Meat Quality ◽  
Treated Group ◽  
Control Group ◽  
Active Principle ◽  
Carcass Quality ◽  
Feed Conversion ◽  
Zinc Gluconate ◽  
Hormone Levels ◽  
Significant Difference

ABSTRACT A non-surgical sterilant (NSS) was used with the objective of assessing the zootechnical performance, carcass and meat quality, and hormone levels. 90 male piglets were selected with birthweights varying between 1.5kg to 2.0kg. The control group was constituted of 45 males castrated by the conventional surgical method, at the seventh day of age, and the treated group was constituted of 45 males castrated with NSS (active principle of zinc gluconate in the concentration of 26.2mg/mL associated to the dimethyl sulfoxide at 0.5%), with the application of the first dose on the seventh day of age and the second dose on the fourteenth day of age. The zootechnical assessments were carried out on the farm and consisted: weight gain in the periods per animal and feed conversion. The slaughter of the animals and the assessment of the carcass and meat quality and hormone levels were carried out in a slaughterhouse and the municipality of Campinas (SP). The study demonstrated that the use of NSS was a viable alternative in relation to the zootechnical performance and the carcass quality. In relation to the meat quality there was no significant difference in the majority of the assessed parameters.

scholarly journals Prophylactic Use of Meloxicam and Paracetamol in Peripartal Sows Suffering From Postpartum Dysgalactia Syndrome

2020 ◽  
Vol 7 ◽  
Author(s):  
Alexandra Schoos ◽  
Ilias Chantziaras ◽  
Jordy Vandenabeele ◽  
Evelien Biebaut ◽  
Evelyne Meyer ◽  
...  
Keyword(s):  
Weight Gain ◽  
Rectal Temperature ◽  
Group Treatment ◽  
Acute Phase Proteins ◽  
Treated Group ◽  
Daily Weight Gain ◽  
Control Group ◽  
Gestation Length ◽  
Amyloid A ◽  
Health Parameters

Postpartum dysgalactia syndrome (PPDS) is a major economic problem in modern sow farms. General treatment of PPDS consists of the use of oxytocin to promote milk ejection and non-steroidal anti-inflammatory drugs (NSAIDs) to alleviate inflammatory processes. So far, studies investigated the use of a single administration of NSAIDs after parturition in healthy and non-healthy sows. The current study investigated whether administration of meloxicam or paracetamol in sows prior to parturition improves sow and piglet health as well as performance in a farm with PPDS problems in sows. Sixty sows and 978 piglets from a Belgian farrow-to-finish farm were enrolled. Sows were randomly divided into three groups: a non-treated control group, a meloxicam-treated group and a paracetamol-treated group. Treatment was administered orally for 7 days from gestation day 113 onwards. Performance and health parameters investigated in sows were gestation length, farrowing duration, litter characteristics, colostrum yield and quality (Immunoglobulin G), litter weight gain, weaning-to-estrus interval, pregnancy rate, rectal temperature, acute phase proteins and inflammatory markers serum amyloid A, haptoglobin, interferon γ, interleukin 1β and 6 backfat, constipation and feed refusal. Performance and health parameters in piglets were birthweight, average daily weight gain, colostrum intake and mortality. Paracetamol-treated sows showed a significantly (P = 0.04) lower rectal temperature (mean ± SD: 38.09 ± 0.18°C) than the meloxicam-treated sows (38.24 ± 0.18°C), but not than the control group (38.22 ± 0.18°C). Sows of the paracetamol-treated group had a significantly (P = 0.001) longer gestation length (116.3 ± 0.9 days) than sows of the control group (115.3 ± 0.6 days), but not than meloxicam-treated sows (115.9 ± 0.9 days). No significant differences between the three groups were found for all the other parameters. In conclusion, the prophylactic oral administration of either meloxicam or paracetamol for 7 days starting 2 days prior to farrowing did not show beneficial effects on both health and performance parameters of sows and piglets.

scholarly journals Chronic NOS Inhibition Affects Oxidative State and Antioxidant Response Differently in the Kidneys of Young Normotensive and Hypertensive Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
M. Majzunova ◽  
M. Kvandova ◽  
A. Berenyiova ◽  
P. Balis ◽  
I. Dovinova ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Oxidative Damage ◽  
Young Male ◽  
Antioxidant Defense System ◽  
Total Activity ◽  
Antioxidant Response ◽  
Control Group ◽  
Hypertensive Rats ◽  
Sod Activity ◽  
Oxidative State

Deficiency of nitric oxide (NO) and oxidative stress can be a cause, a consequence, or, more often, a potentiating factor for hypertension and hypertensive renal disease. Both NO and superoxide anions are radical molecules that interact with each other, leading to oxidative damage of such organs as the kidney. In the present study, we investigated the effect of chronic-specific (neuronal NOS inhibition) and nonspecific NOS inhibition on the oxidative state and antioxidant response and associated oxidative damage of the kidney of young normotensive and hypertensive rats. Young male normotensive Wistar rats (WRs, age 4 weeks) and spontaneously hypertensive rats (SHRs, age 4 weeks) were divided into three groups for each strain by the type of administered compounds. The first group was treated with 7-nitroindazole (WR+7-NI; SHR+7-NI), the second group was treated with N(G)-nitro-L-arginine-methyl ester (WR+L-NAME; SHR+L-NAME), and the control group was treated with pure drinking water (WR; SHR) continuously for up to 6 weeks. Systolic blood pressure increased in WR+L-NAME after the first week of administration and increased slightly in SHR+L-NAME in the third week of treatment. 7-NI had no effect on blood pressure. While total NOS activity was not affected by chronic NOS inhibition in any of the WR groups, it was attenuated in SHR+7-NI and SHR+L-NAME. Nitration of proteins (3-nitrotyrosine expression) was significantly reduced in WR+7NI but not in WR+L-NAME and increased in SHR+7-NI and SHR+L-NAME. Immunoblotting analysis of SOD isoforms showed decreased SOD2 and SOD3 expressions in both WR+7-NI and WR+L-NAME followed by increased SOD activity in WR+L-NAME. Conversely, increased expression of SOD2 and SOD3 was observed in SHR+L-NAME and SHR+7-NI, respectively. SOD1 expression and total activity of SOD did not change in the SHR groups. Our results show that the antioxidant defense system plays an important role in maintaining the oxidative state during NO deficiency. While the functioning antioxidant system seeks to balance the oxidation state in the renal cortex of normotensive WRs, the impaired antioxidant activity leads to the development of oxidative damage of proteins in the kidney induced by peroxynitrite in SHRs.

scholarly journals Intestinal healing in rats submitted to ethanol ingestion

2012 ◽  
Vol 27 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Rodrigo Severo de Camargo Pereira ◽  
Cláudia Nishida Hasimoto ◽  
Leonardo Pelafsky ◽  
Juan Carlos Llanos ◽  
Daniele Cristina Cataneo ◽  
...  
Keyword(s):  
Weight Gain ◽  
Postoperative Complications ◽  
Breaking Strength ◽  
Surgical Site Infections ◽  
Treated Group ◽  
Ethanol Ingestion ◽  
Control Group ◽  
Histopathological Study ◽  
Study Results ◽  
Lower Weight Gain

PURPOSE: To study the effect of alcoholism on intestinal healing and postoperative complications in rats METHODS: One hundred and sixty rats were divided into two groups: control and treated. The control group received water and the treated group 30% ethanol. After 180 days, colotomy with anastomosis were performed. After, the groups were divided into four subgroups: 20 rats for study at the following moments: 4th, 7th, 14th and 21st postoperative. The analyzed parameters were: weight gain, breaking strength, tissue hydroxyproline, postoperative complications and histopathological study RESULTS: Weight gain was greater in the control group (p<0.05). When all the subgroups were clustered, breaking strength was significantly greater in the control (p<0.05). Histopathology and hydroxyproline dosage did not show differences. There were five surgical site infections in the treated group while the control group showed two (p>0.05). Nine fistulas occurred in the treated group whereas the control group two (p<0.05). There were three deaths in the control group and seven in the treated group (p>0.05). CONCLUSIONS: Treated group undergo a malnutrition process that is revealed by lower weight gain. Impaired intestinal healing as indicated by smaller breaking strength. There were a larger number of postoperative complications in the treated animals.

scholarly journals Oral Meloxicam Administration in Sows at Farrowing and Its Effects on Piglet Immunity Transfer and Growth

2021 ◽  
Vol 8 ◽  
Author(s):  
Elena Navarro ◽  
Eva Mainau ◽  
Ricardo de Miguel ◽  
Déborah Temple ◽  
Marina Salas ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Weight Gain ◽  
Maternal Behavior ◽  
Interleukin 2 ◽  
Treated Group ◽  
Interleukin 4 ◽  
Control Group ◽  
Piglet Serum ◽  
Cellular Immune

Many factors can lead to an inadequate development of piglets during their first days of life, including poor maternal behavior, which can be due to pain caused by farrowing, and reduced colostrum ingestion. This study investigates the action of meloxicam administered orally at farrowing on piglet weight gain and immunity transfer. Thirty-five multiparous sows were divided into two groups and treated with 0.4 mg/kg of oral meloxicam (oral meloxicam group; n = 18) or with a mock administration (control group; n = 17). A total of 382 piglets were individually weighed on the farrowing day (day 0), as well as on days +9 and +20. Immunoglobulin G (IgG) and A (IgA) concentrations in piglet serum and in sow's saliva, colostrum and milk were measured. Additionally, Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Interferon gamma (IFN-⋎) in serum of piglets and in sow's milk or colostrum were studied. All samples were obtained on days +1, +9, and +20. Piglets from sows in the oral meloxicam group tended to grow faster from day +9 to day +20 than did piglets from control sows (p = 0.059), and this difference was also observed in piglets with low body weight (BW) at birth (p = 0.056). The oral meloxicam group sows tended to increase the colostrum levels of IgA and IgG, as compared with control sows on day +1 (p = 0.068 and p = 0.072, respectively). IgA levels in piglet serum from the oral meloxicam group were significantly higher than in the control group on day +1 and +9 (p = 0.019 and p = 0.011 respectively). Furthermore, IL-2 and IL-4 levels in the serum of piglets from sows in the oral meloxicam group tended to be higher than that in the control group on day +9 (p = 0.078 and 0.056, respectively). The administration of meloxicam orally at the beginning of farrowing in multiparous sows increased immunoglobin and cytokine concentrations in colostrum, improving both humoral and cellular immune response of piglets. Pre-weaning growth of piglets born with a low BW improved in the meloxicam-treated group.

scholarly journals Effect of spirulina (Spirulina platensis) and vitamin E on arsenic induced toxicity in Quail

2020 ◽  
Vol 6 (1) ◽  
pp. 93-98
Author(s):  
Most Fayza Khatun ◽  
Md Mahmudul Hasan ◽  
Rakibul Islam ◽  
Sumon Sarkar ◽  
Md Anowarul Haque
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Vitamin E ◽  
Arsenic Trioxide ◽  
Spirulina Platensis ◽  
Body Weight Gain ◽  
Treated Group ◽  
Control Group ◽  
Arsenic Toxicity ◽  
Histopathological Changes

Chronic arsenic toxicity is a severe disease in men and animals which occurs severely in Bangladesh. Arsenic (As) contamination in ground water used in drinking is the major concern because arsenic is present in human and animal food chain. This work was done in quails with a view to observing the efficacy of spirulina (Spirulina platensis) and vitamin E for prevention of arsenic toxicity. 60 quails were used in this study and animals were divided into control group (T0), arsenic treated group (T1), arsenic plus spirulina treated group (T2) and arsenic plus vitamin E treated group (T3). Each group consists of 15 quails. Quails of T0 group were given normal feed and water and kept as control. Quails of T1, T2 and T3 were given 100 mg arsenic trioxide/L drinking water daily for 30 days. In addition to arsenic trioxide quails of group T2 and T3 were simultaneously fed with spirulina @ 1 gm/kg feed and vitamin E @ 400 mg /kg body weight up to 30 days respectively. Five quails from each group (T0, T1, T2 and T3) were sacrificed at 15 days interval in order to determine haematological parameters. Result showed that in group T1, body weight gain was minimum, whereas in group T2 and T3 the body weight gain in quails were better. Reduction of TEC and Hb values were observed in arsenic treated group T1. Whereas in rest groups the TEC and Hb values were comparatively higher than arsenic treated group. Noticeable change observed in liver and kindey of arsenic treated group in compare to the control group. Histopathological changes also observed in liver and kindey of arsenic treated group in compare to the control group. In conclusion, spirulina and vitamin E have significant effect on body weight, hematological and postmortem and histopathological changes. Asian J. Med. Biol. Res. March 2020, 6(1): 93-98

scholarly journals Anticancer activity of ylang-ylang essential oil in Ehrlich Ascites Carcinoma cell-treated mice

2020 ◽  
Vol 11 (4) ◽  
pp. 5752-5762
Author(s):  
Ajay P Malgi ◽  
Vijaykumar P Rasal ◽  
Vishal Shivalingappa Patil ◽  
Priyanka P Patil ◽  
Shamanad P Mallapur ◽  
...  
Keyword(s):  
Essential Oil ◽  
Complex Mixture ◽  
Ehrlich Ascites Carcinoma ◽  
Treated Group ◽  
Ehrlich Carcinoma ◽  
Control Group ◽  
Sod Activity ◽  
Standard Drug ◽  
Chemical Structures ◽  
Essential Components

Essential oils are secondary metabolites contains a complex mixture of with a diverse array of chemical structures, play a crucial role in the management of complex diseases like cancer via synergistic and antagonism effect. essential oil (YYEO) extracted from the flowers of Cananga is renowned forits fragrance, contained more than 150 essential components within it, and utilized in various diseases and cosmetics. Traditionally YYEO is being used as an aphrodisiac, , , antiseptic, in food and beverages as a fragrance agent. Due to the presence of a complex mixture of essential components in YYEO, we aimed the current study to assess the anticancer potential against Ehrlich Carcinoma (EAC) bearing mice. In antioxidant, growth, body weight, biochemical, , and serum estimation was evaluated with subsequent of the liver. 5- (5- FU) was used as a standard drug. YYEO showed potent antioxidant activity by DPPH assay. YYEO significantly reversed the , lymphocytes, WBC, and RBC numbers in the treated group compared with the disease control group. YYEO administration has restored the imbalanced levels of antioxidant such as MDA, GSH, and SOD activity. YYEO reversed the of the liver altered by the EAC in mice. In conclusion, a complex mixture of contained in YYEO could be the potent anticancer therapy in the future. Further studies are needed to identify the active principles and the mechanism involved in this activity.

scholarly journals Effects of iodine formulations on body weight and haematological parameters in beef cattle

1970 ◽  
Vol 4 (2) ◽  
pp. 133-135
Author(s):  
MR Sultana ◽  
M Mostofa ◽  
MA Awal ◽  
MMH Sikder ◽  
MA Hossain
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Beef Cattle ◽  
Dairy Farm ◽  
Treated Group ◽  
Blood Parameters ◽  
Control Group ◽  
Meat Production ◽  
Lugol’S Iodine ◽  
Group B

The present research was carried out on indigenous beef cattle to study the effect of iodine formulations on body weight and hematology by using iodide salt and Lugol's iodine irrespective of feed and management in dairy farm, Bangladesh Agricultural University in collaboration with the Department of Pharmacology, Bangladesh Agricultural University for the period of 2 months from August to September 2005. A total of 20 male bull of 2 years old were selected, weighed ranged from 80-100kg and randomly divided into four groups as A, B, C and D. Group A was treated with non- iodide salt @ 25mg/ kg b.wt, group B treated with iodide salt @ 25mg/ kg b.wt, group C treated with IV injection of 2 % Lugol's iodine @ 10 mg/kg b. wt and group D were kept as control. After treatment, blood parameters and body weight were recorded on 30th and 60th day. Highest weight gain was recorded in Lugol's iodine treated group, about 7.09 % for weight gain compared with the control group. The result reveals that use of iodine formulation is an effective way of increasing meat production and weight gain in indigenous beef cattle of Bangladesh. Key Words: Meat production, body weight, indigenous beef cattle, iodide salt, Lugol's iodine DOI = 10.3329/bjvm.v4i2.1297 Bangl. J. Vet. Med. (2006). 4 (2): 133-135

scholarly journals Effects of organic zinc supplementation in weaned calves

2016 ◽  
Vol 85 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Sylva Dresler ◽  
Josef Illek ◽  
Ladislav Zeman
Keyword(s):  
Metabolic Profile ◽  
Zinc Supplementation ◽  
Serum Zinc ◽  
Treated Group ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Sod Activity ◽  
Organic Zinc ◽  
Zinc Concentrations ◽  
Organic Zn

The aim of the study was to investigate the effect of organic zinc supplementation in calves on serum zinc (Zn) concentrations, selected metabolic profile indicators and serum immunoglobulin (Ig) concentrations. The trial included 2 groups (n = 10) of weaned female calves. The Zn-Methionin calves (group Zn-Met) were supplemented with 30 mg Zn-Met/kg dry matter (DM)/day (BIOPLEX® Zn, Alltech, USA) for 90 days; the control calves (group C) received the same diet without organic zinc supplementation. Compared to the control group, organic Zn treatment significantly increased serum Zn concentration (P < 0.05), superoxide dismutase (SOD) activity (P < 0.01) and total Ig (P < 0.01) in the group Zn-Met at the beginning (7 days from the start of Zn-Met supplementation) of the trial. At the end of the trial (day 90) serum total protein (TP) (P < 0.05), albumin (P < 0.01), urea (P < 0.01), SOD (P < 0.01), copper (Cu) (P < 0.05), Zn (P < 0.01) and Ig (P < 0.05) concentrations were significantly higher in the Zn-Met calves. In the control group alkaline phosphatase (ALP) activity was significantly (P < 0.01) higher on day 90. A positive correlation between zinc concentrations, ALP, and SOD activities in serum, and a negative correlation between zinc and copper concentrations were demonstrated. Dietary Zn-Met supplementation in calves markedly influenced the metabolic profile and serum immunoglobulin concentrations. Compared to the control group, the Zn supplemented group showed a significantly (P < 0.05) lower ALP and significantly greater SOD serum activity (P < 0.01) at the end of the trial. Total Ig concentrations were significantly higher in the Zn treated group (day 7: P < 0.01 vs. day 90: P < 0.05).

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