scholarly journals Evaluation of Toxoplasma, Rubella, and Cytomegalovirus serological results in women of childbearing age

2020 ◽  
Vol 66 (6) ◽  
pp. 789-793
Author(s):  
Fatma Avcioglu ◽  
Mustafa Behcet ◽  
Muhammet Guzel Kurtoglu
Keyword(s):  
Toxoplasma Gondii ◽  
Primary Infection ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Congenital Infections ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Antibody Levels ◽  
University Training

SUMMARY OBJECTIVE This study aimed to determine the rates of IgG and IgM antibodies against cytomegalovirus, rubella, and Toxoplasma gondii (all of which may cause congenital infections) in women of childbearing age who were admitted to Bolu Abant İzzet Baysal University Training and Research Hospital. METHODS Between January 2015 and December 2017, Toxoplasma gondii, rubella, and cytomegalovirus IgM and IgG antibody levels were studied using the ELISA method (Architect i2000SR, Abbott, Germany) in patients aged 15 to 45 who attended the obstetrics and gynecology outpatient clinics. Toxoplasma gondii and cytomegalovirus IgG avidity levels were analyzed retrospectively. RESULTS A total of 13.470 tests were conducted in the laboratory. Seropositivity percentages of IgM antibodies were found to be 1.3%, 0.5%, and 1.6% for Toxoplasma (n = 3607), rubella (n = 3931), and cytomegalovirus (n = 3795), respectively. The seropositivity percentages of IgG antibodies were 22%, 94.2%, and 98.2% for Toxoplasma (n = 702), rubella (n = 693), and cytomegalovirus (n = 679), respectively. Primary infection (acute, recently acquired) was found in 7 (35%) patients with low Toxoplasma IgG avidity. One (3%) patient with low cytomegalovirus IgG avidity had a primary infection. CONCLUSION Toxoplasma gondii seronegativity was found to be high in the region. Therefore, screening women of childbearing age may be important for the prevention of congenital infections caused by Toxoplasma gondii.

EVALUATION OF Toxoplasma gondii SEROPREVALENCE AND IGG AVIDITY TEST RESULTS AMONG CHILDBEARING-AGE WOMEN IN KARABUK PROVINCE

2021 ◽  
Vol 6 (12) ◽  
pp. 98-103
Author(s):  
Meryem COLAK ◽  
Nergis ASGIN
Keyword(s):  
Toxoplasma Gondii ◽  
Congenital Toxoplasmosis ◽  
Epidemiological Data ◽  
Test Results ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Seropositivity Rate ◽  
Igg Avidity ◽  
Avidity Test ◽  
Childbearing Age Women

Toxoplasmosis is a zoonotic infectious disease that is common worldwide and caused by the Toxoplasma gondii. Congenital toxoplasmosis is one of the major complications of this infection. The present study aimed to investigate T.gondii seroprevalence and evaluate the IgG avidity test results among childbearing- age women retrospectively. A total of 9401 samples obtained from childbearing -age (15-49 years of age) women who attended Karabuk University Training and Research Hospital between February 2016- January 2020, over the four-year, were included in the study.The Toxoplasma-IgM, Toxoplasma-IgG antibody, and Toxoplasma IgG avidity tests were analyzed chemiluminescent method by using ARCHITECT I 2000 SR immunoassay device (Abbott Laboratories, USA). Toxoplasma-IgM and Toxoplasma-IgG seropositivity were detected as 0.7% (67/9401) and 15.7% (1415/9005) respectively. A high percentage of Toxoplasma-IgM and Toxoplasma-IgG seropositivity were detected among 31-35 years of age group. The Toxoplasma seropositivity increased by age, but no statistically difference was found (P >0.05).The Toxoplasma IgG avidity was high in 45 patients (69.4%), low avidity was detected in 16 patients (22.6%) and from the remaining 6 patients were borderline (8%). The Toxoplasma IgM and IgG seropositivity rate decreased 2016 to 2020. The highest Toxoplasma IgM and IgG seropositivity were in 2016 with 1.2% and 16.8%, respectively. The T. gondi seropositivity rate was decreased from 2016 to 2020; it is probably a result of increasing awareness of the disease among patients. We think our results will contribute to the epidemiological data in our province and country and raise awareness.

scholarly journals Prevalence of IgG antibodies specific to Toxoplasma gondii among blood donors in Recife, Northeast Brazil

2003 ◽  
Vol 45 (4) ◽  
pp. 229-231 ◽  
Author(s):  
Raquel A.L. Coêlho ◽  
Masashi Kobayashi ◽  
Luiz B. Carvalho Jr.
Keyword(s):  
Toxoplasma Gondii ◽  
Enzyme Immunoassay ◽  
Blood Donors ◽  
Northeast Brazil ◽  
Serological Survey ◽  
Igg Antibodies ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Toxoplasma Gondii Infection

A serological survey of Toxoplasma gondii infection in blood donors was carried out in order to identify seroprevalence in Recife, Brazil. Sera from 160 individuals (119 male and 41 female) were evaluated by using a Toxoplasma IgG-antibody enzyme immunoassay (Denka Seiken Co., LTD., Tokyo, Japan). The seropositive percentual for males (79.0%) showed to be higher (p < 0.05) than for females (63.4%). This percentage increases with age, ranging from 18.2% to 92.6% for individuals aging under 20 and 40-50 years old, respectively. For women of childbearing age (18-40 years) it was found a prevalence of 51.6%.

scholarly journals Association between Toxoplasma gondii infection and history of blood transfusion: a case-control seroprevalence study

2018 ◽  
Vol 46 (4) ◽  
pp. 1626-1633 ◽  
Author(s):  
Cosme Alvarado-Esquivel ◽  
Luis Francisco Sánchez-Anguiano ◽  
Jesús Hernández-Tinoco ◽  
Agar Ramos-Nevarez ◽  
Sergio Estrada-Martínez ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Blood Transfusion ◽  
Igg Antibody ◽  
Igm Antibodies ◽  
Significant Difference ◽  
History Of ◽  
The Difference ◽  
Toxoplasma Gondii Infection ◽  
Antibody Levels ◽  
Positive Controls

Objectives This study was performed to determine the association between seropositivity to Toxoplasma gondii and a history of blood transfusion. Methods Patients who had undergone blood transfusion (n = 410) and age- and sex-matched controls who had not undergone blood transfusion (n = 1230) were examined for anti- T. gondii IgG and IgM antibodies using enzyme-linked immunoassays. Results Anti- T. gondii IgG antibodies were detected in 57 (13.9%) patients and in 129 (10.5%) controls with a borderline difference [odds ratio (OR) = 1.37, 95% confidence interval (CI) = 0.98–1.92]. High anti- T. gondii IgG antibody levels (>150 IU/mL) were found in 27 (47.4%) of the 57 anti- T. gondii IgG-positive patients and in 37 (28.7%) of the 129 anti- T. gondii IgG positive controls with a significant difference (OR = 2.23, 95% CI = 1.17–4.26). Anti- T. gondii IgM antibodies were found in 13 (22.8%) of the 57 seropositive patients and in 37 (28.7%) of the 129 seropositive controls, but the difference was not significant (OR = 0.73, 95% CI = 0.35–1.52). Seroprevalence was significantly higher in patients aged >50 years than in controls of the same age and in female patients than in female controls. Conclusions These findings indicate that a history of blood transfusion is a risk factor for T. gondii infection.

scholarly journals Seroepidemiological study of toxoplasmosis in women referred to a pre-marriage counseling center in Alborz Province, Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Melica Shahighi ◽  
Aliehsan Heidari ◽  
Hossein Keshavarz ◽  
Amir Bairami ◽  
Saeedeh Shojaee ◽  
...  
Keyword(s):  
Congenital Toxoplasmosis ◽  
Pcr Analysis ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Serum Samples ◽  
Chi Square ◽  
Igg Avidity ◽  
Two Samples ◽  
Chi Square Test ◽  
Associated Risk Factors

Abstract Objectives The aim of the current study was to assess prevalence of Toxoplasma infection and its associated risk factors in women of childbearing-age in central Iran. Results Of 400 serum samples assessed for anti-T. gondii antibodies, 81 (20.25%) samples were positive for anti-T. gondii antibodies, including 74 positive samples (91.3%) for anti-T. gondii IgG and seven positive samples (8.7%) for IgG and IgM. Of seven IgG and IgM positive samples, five and two samples were high and low in IgG avidity, respectively. Based on PCR analysis, Toxoplasma infection was detected in one sample with anti-T. gondii IgM and low IgG avidity. The Chi-square test showed significant correlations of T. gondii seropositivity with history of undercooked meat consumption and contacts with cats (p < 0.05). In the present study, 79.75% of the participants were negative for IgG against T. gondii infection. Furthermore, recently acquired Toxoplasma infection was found using IgG avidity and PCR assays among women of childbearing-age in the study area, which would increase the risk of their fetus becoming infected. Educational program and antenatal screening of childbearing-age women for T. gondii infection may be important primary prevention strategies and help reduce the risk of congenital toxoplasmosis in this population.

scholarly journals Evaluation of the TGS TA system for the detection of anti-rubella antibodies

2017 ◽  
Vol 32 (2) ◽  
Author(s):  
Olivia Arpino ◽  
Annalisa Cianflone ◽  
Maria Teresa Manco ◽  
Alessia Paganini ◽  
Massimo De Paschale ◽  
...  
Keyword(s):  
Elisa Test ◽  
Childbearing Age ◽  
Past Infection ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Group 3 ◽  
Group 2 ◽  
Sera Samples ◽  
Group 1

<p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study), TGS TA system was compared to an ELFA IgM test and with an ELISA test for the IgG avidity (if existent).<br /><em>Materials and methods:</em> Two hundred and seventy six sera samples from women were examined with TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 112 were of childbearing age and non-immune women (Group 1), 106 were pregnant with past infection or vaccinated (Group 2) and 49 were pregnant with positive or equivocal IgM (Group 3). <br /><em>Results</em>: The overall concordance of the IgG results between LIAISON XL and TGS TA was 93.3%: 86.6% in Group 1, 97.2% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 89.0%: 100% in Group 1, 100% in Group 2 and 35.6% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELISA and TGS TA tests was 85.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 97.4%: 86.6% in Group 1, 97.2% in Group 2 and 85.7% in Group 3. <br /><em>Conclusions</em>: TGA TS system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection or those who are vaccinated. The TGS TA test also seems to be especially sensitive in indicating vaccinated subjects with low IgG levels as immune.</p>

scholarly journals Recent trends in seroprevalence of rubella in Korean women of childbearing age: a cross-sectional study

BMJ Open ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. e030873 ◽  
Author(s):  
Rihwa Choi ◽  
Yejin Oh ◽  
Youngju Oh ◽  
Sung Ho Kim ◽  
Sang Gon Lee ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Korean Women ◽  
Test Results ◽  
Women Of Childbearing Age ◽  
Sectional Study ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Specific Igg ◽  
Catch Up

ObjectivesThe aim of this study was to investigate the immunity against rubella using the serological status of rubella-specific IgG antibodies (antirubella IgG) in Korean women of childbearing age (15–49 years).DesignRetrospective cross-sectional study.SettingPopulation-based cross-sectional study in South Korea.ParticipantsBetween January 2010 and December 2017, test results from Korean women aged 15–49 years who had visited an obstetric private clinic (nationwide institutions) and had requested rubella-specific IgG antibody tests from Green Cross Laboratories were obtained from the laboratory information system.ResultsBetween 2010 and 2017, antirubella IgG test results from 328 426 Korean women aged 15–49 years who had visited private obstetric clinics (1438 institutions nationwide) were retrospectively analysed by tested year, age, cohort and geographic regions. Over the 8-year study period, the rate of unimmunised women ranged from 7.8% to 9.7%. Multivariable-adjusted logistic regression models showed that the odds of being immune to rubella (positive and equivocal results of antirubella IgG test) were lower in 2017 compared with 2010, in women in their 40s, in a pre-catch-up cohort and in women living in Incheon, Busan, South Gyeongsang, North and South Jeolla and Jeju provinces (p<0.0001).ConclusionsIn consideration of the factors associated with prevalence of women unimmunised to rubella, future public health efforts should be focused on catch-up activities. The results of this study could be used to strengthen disease control and prevent rubella, including a nationwide immunisation programme.

scholarly journals An observational study of the reactogenicity and immunogenicity of 13-valent pneumococcal conjugate vaccine in women of childbearing age in Papua New Guinea

Pneumonia ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Sarah Javati ◽  
Geraldine Masiria ◽  
Arthur Elizah ◽  
John-Paul Matlam ◽  
Rebecca Ford ◽  
...  
Keyword(s):  
High Risk ◽  
Observational Study ◽  
Conjugate Vaccine ◽  
Pneumococcal Conjugate Vaccine ◽  
Early Life ◽  
Women Of Childbearing Age ◽  
Pneumococcal Infections ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Young Infants

Abstract Background Maternal immunization with pneumococcal conjugate vaccine (PCV) may protect young infants in high-risk settings against the high risk of pneumococcal infections in early life. The aim of this study was to determine the safety and immunogenicity of 13-valent PCV (PCV13) in healthy women of childbearing age in PNG. Methods As part of this observational study, 50 non-pregnant women of childbearing age (18-45 yrs. old) living in the highlands of PNG were vaccinated with a single dose of PCV13. Local and systemic reactogenicity were assessed 24–48 h after vaccination. Venous blood samples were collected before and 1 month after vaccination to measure PCV13 serotype-specific IgG antibody concentrations. Results No severe adverse effects were reported during the 1-month follow-up period. IgG antibody concentrations significantly increased after vaccination for all PCV13 serotypes. One month after vaccination IgG antibody levels ≥2.5 μg/mL were reached in at least 75% of women for all PCV13 serotypes, except serotype 3, and ≥ 5 μg/mL in at least 75% of women for 7 serotypes (serotypes 6B, 9 V, 14, 18C, 19A, 19F and 23F). Conclusion PCV13 is safe and immunogenic in women of childbearing age living in a high-risk setting in PNG. This supports the implementation of studies to investigate the safety and immunogenicity of maternal PCV vaccination in high-risk settings as a strategy to protect infants in these settings against the high risk of pneumococcal infections in early life. Trial registration NCT04183322. Registered 3 December 2019 - Retrospectively registered

scholarly journals Toxoplasma gondii in women of childbearing age and during pregnancy: seroprevalence study in Central and Southern Italy from 2013 to 2017

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 2 ◽  
Author(s):  
Daniela Fanigliulo ◽  
Serena Marchi ◽  
Emanuele Montomoli ◽  
Claudia Maria Trombetta
Keyword(s):  
Toxoplasma Gondii ◽  
Pregnant Women ◽  
Southern Italy ◽  
Central Italy ◽  
Neonatal Infection ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Serum Samples ◽  
Age Related ◽  
Significant Difference

Toxoplasmosis is a worldwide health problem. Infection in pregnant women can result in severe fetal morbidity or in subclinical neonatal infection; most subclinical cases develop ocular and neurological sequelae. The purpose of this serological study was to assess the prevalence of Toxoplasma gondii in two populations of women of childbearing age in Siena (Tuscany, Central Italy) and Bari (Apulia, Southern Italy) between 2013 and 2017 and in a group of pregnant women in Bari in 2016–2017. Serum samples were tested for the presence of specific anti-Toxoplasma gondii IgG antibodies by a commercially available ELISA test. The percentage of seropositive subjects in Bari was significantly higher than in Siena (22.4% vs. 12.4%) and an age-related trend was observed. A low prevalence of T. gondii infection (13.8%) was observed among the pregnant women tested. In addition to showing a significant difference between Central and Southern Italy, this study provides updated data on T. gondii seroprevalence in women during childbearing age and pregnancy. The results confirm a trend toward a decrease, especially in younger people and pregnant women.

scholarly journals Risk factors for Toxoplasma gondii infection in women of childbearing age

2004 ◽  
Vol 8 (2) ◽  
Author(s):  
Mariza Martins Avelino ◽  
Dioclécio Campos Júnior ◽  
Josetti Barbosa de Parada ◽  
Ana Maria de Castro
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Toxoplasma Gondii Infection

scholarly journals Rubella and measles seroprevalence among women of childbearing age, Argentina, 2002

2005 ◽  
Vol 133 (5) ◽  
pp. 861-869 ◽  
Author(s):  
G. H. DAYAN ◽  
M. S. PANERO ◽  
A. URQUIZA ◽  
M. MOLINA ◽  
S. PRIETO ◽  
...  
Keyword(s):  
Public Health Care ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Convenience Sample ◽  
Younger Women ◽  
Congenital Rubella ◽  
Antibody Titres ◽  
Measles Outbreaks

To assess rubella and measles susceptibility among women of childbearing age we conducted a cross-sectional seroprevalence study in four cities and one rural area in Argentina. A convenience sample of women aged 15–49 years seeking care in public health-care institutions was selected (n=2804). Serum specimens were tested for rubella and measles IgG antibody titres. The overall susceptibility to rubella and measles was 8·8 and 12·5% respectively. Seroprevalence differences were found for both rubella (P<0·001) and measles (P=0·002) across sites. Rubella seroprevalence was higher in women aged [ges ]40 years than in younger women (P=0·04). Measles seroprevalence tended to increase with age (P<0·001). Approximately 15% of women aged 15–29 years were not immune to measles. No risk factors were associated with rubella seronegativity; however, age (P<0·001) and having less than four pregnancies (P<0·001) were factors associated with measles seronegativity. Our findings support the introduction of supplemental immunization activities targeting adolescents and young adults to prevent congenital rubella syndrome and measles outbreaks over time.

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