scholarly journals The anti-inflammatory and antinociceptive effects of proteins extracted from Acacia farnesiana seeds

2016 ◽  
Vol 18 (1) ◽  
pp. 38-47 ◽  
Author(s):  
L.S.S. LEAL ◽  
R.O. SILVA ◽  
T.S.L. ARAUJO ◽  
V.G. SILVA ◽  
A.L.R. BARBOSA ◽  
...  
Keyword(s):  
Protein Pattern ◽  
Antinociceptive Effect ◽  
Northeastern Brazil ◽  
Myeloperoxidase Activity ◽  
Dependent Manner ◽  
Hot Plate Test ◽  
Acacia Farnesiana ◽  
Anti Inflammatory ◽  
Pre Treatment ◽  
Analgesic Activities

ABSTRACT Seeds of Acacia farnesiana are commonly sold in the local markets of northeastern Brazil as a therapeutic agent. The present work aimed to evaluate the anti-inflammatory and analgesic activities of proteins obtained from A. farnesiana seeds. Five different protein fractions (albumin, globulin, prolamin, acidic and basic glutelins) were obtained and investigated for the protein pattern, the presence of hemagglutinating and proteolytic activities. The globulin fraction (GLB) was also evaluated for anti-inflammatory and analgesic activities. Globulins reduced the paw edema induced by carrageenan in a dose-dependent manner, which was accompanied by a reduction of myeloperoxidase activity (p < 0.05). Additionally, GLB reduced the neutrophil peritoneal migration induced by carrageenan. However, GLB was not able to inhibit the edema triggered by dextran. Pre-treatment with globulins reduced the abdominal constrictions induced by acetic acid as well as the paw licking time induced by formalin (69.1% at first phase). However, it did not produce a significant antinociceptive effect in the hot plate test (55-56 °C). Treating the GLB with heat (at 100 °C for 30 min) abolished its anti-edematogenic and hemagglutinating activities. Our results showed that seeds from A. farnesiana are a source of proteins with anti-inflammatory and analgesic properties.

scholarly journals Efficacy of Posidonia oceanica Extract against Inflammatory Pain: In Vivo Studies in Mice

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 48
Author(s):  
Laura Micheli ◽  
Marzia Vasarri ◽  
Emanuela Barletta ◽  
Elena Lucarini ◽  
Carla Ghelardini ◽  
...  
Keyword(s):  
Inflammatory Pain ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Posidonia Oceanica ◽  
In Vivo Studies ◽  
Myeloperoxidase Activity ◽  
Intraplantar Injection ◽  
Hot Plate Test ◽  
Anti Inflammatory

Posidonia oceanica (L.) Delile is traditionally used for its beneficial properties. Recently, promising antioxidant and anti-inflammatory biological properties emerged through studying the in vitro activity of the ethanolic leaves extract (POE). The present study aims to investigate the anti-inflammatory and analgesic role of POE in mice. Inflammatory pain was modeled in CD-1 mice by the intraplantar injection of carrageenan, interleukin IL-1β and formalin. Pain threshold was measured by von Frey and paw pressure tests. Nociceptive pain was studied by the hot-plate test. POE (10–100 mg kg−1) was administered per os. The paw soft tissue of carrageenan-treated animals was analyzed to measure anti-inflammatory and antioxidant effects. POE exerted a dose-dependent, acute anti-inflammatory effect able to counteract carrageenan-induced pain and paw oedema. Similar anti-hyperalgesic and anti-allodynic results were obtained when inflammation was induced by IL-1β. In the formalin test, the pre-treatment with POE significantly reduced the nocifensive behavior. Moreover, POE was able to evoke an analgesic effect in naïve animals. Ex vivo, POE reduced the myeloperoxidase activity as well as TNF-α and IL-1β levels; further antioxidant properties were highlighted as a reduction in NO concentration. POE is the candidate for a new valid strategy against inflammation and pain.

scholarly journals Antipyretic, Analgesic and Anti-inflammatory Activity of Qurs Afsanteen Saghir (A Polyherbal Unani Tablet) in Experimental Animals

2022 ◽  
Author(s):  
MD Maseehullah ◽  
Gulam Mohammed Husain ◽  
Mohammed Zakir ◽  
Mohd Kashif Husain ◽  
Ghazala Javed ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Study Drug ◽  
Prunus Dulcis ◽  
Inflammatory Activity ◽  
Apium Graveolens ◽  
Dependent Manner ◽  
Test Drug ◽  
Artemisia Absinthium ◽  
Hot Plate Test ◽  
Anti Inflammatory

Qurs Afsanteen Saghir is a polyherbal Unani formulation in the form of tablet. This formulation consists of multiple medicinal plants like Afsanteen (Artemisia absinthium L.), Badam Talkh (Prunus dulcis (Mill.) D.A.Webb), Asaroon (Asarum europaeum L.), Anisoon (Pimpinella anisum L.) and Tukhm-e-Karafs (Apium graveolens L.). The clinical adult dose of study drug is 3.5 –7 g per day as mentioned in Unani literature. The present study evaluated the antipyretic, analgesic and anti-inflammatory potential of Qurs Afsanteen Saghir using different animal models. Antipyretic activity was measured using yeast-induced pyrexia model in rats at 360 and 720 mg/kg bw dose of test drug and paracetamol (70 mg/kg bw p.o.) as standard control. Analgesic effect was evaluated using acetic acid-induced writhing test in mice using test drug at dose 720 and 1440 mg/kg bw and diclofenac sodium (15 mg/kg bw p.o.) as standard control. Eddy’s hot plate test was conducted in rats using test drug at the dose of 360 and 720 mg/kg bw and buprenorphine (0.10 mg/kg s.q.) as standard control. Anti-inflammatory activity was assessed by carrageenan-induced paw edema model in rats with the dose of 360 and 720 mg/kg of test drug and Indomethacin (10 mg/kg p.o.) as standard control. The study drug significantly reduced the temperature and pain at both dose levels in a time-dependent manner as compared to normal control. However, the reduction of inflammation was observed at low dose (360 mg/kg bw) only after 3 hours of carrageenan administration. These findings indicated that tested drug showed potential activity as antipyretic and analgesic; whereas the drug may not be considered quite effective as an anti-inflammatory agents.

scholarly journals Basal anti-inflammatory responses in cultured endothelial cells exposed to two conjugated linoleic acids

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Carina Valenzuela ◽  
Elizabeth Miles ◽  
Philip Calder
Keyword(s):  
Inflammatory Responses ◽  
Adhesion Assay ◽  
Dependent Manner ◽  
Relative Expression ◽  
Inflammatory Pathways ◽  
Expression Of Genes ◽  
Low Concentrations ◽  
Genes Encoding ◽  
Anti Inflammatory ◽  
Pre Treatment

AbstractConjugated linoleic acid (CLA) isomers have been shown to possess anti-atherosclerotic properties, which may be related to the downregulation of inflammatory pathways. Whether low concentrations of CLAs are able to affect basal, unstimulated endothelial cell (EC) responses is not clear. The aim of this study was to evaluate the effects of two CLAs (cis-9, trans-11 and trans-10, cis-12) on basal inflammatory responses by ECs.EA.hy926 cells (HUVEC lineage) were cultured under standard conditions and exposed to CLAs (1 and 10 μM) for 48 hours. MTT assay was performed to determine cell viability; incorporation of FA was confirmed by gas chromatography; inflammatory mediators were assessed by multiplex immunoassay; the relative expression of genes encoding transcription factors and inflammatory cytokines was assessed through real-time PCR and static adhesion assay was used to evaluate monocyte attachment to the EC monolayer.CLAs were incorporated into ECs in a dose-dependent manner. Pre-treatment with CLA9,11 (1 uM) significantly reduced unstimulated, basal concentrations of MCP-1 (p < 0.05), and CLA10,12 at 10 uM had the same effect (p < 0.05). Both CLAs at 10 uM increased the relative expression of NFκβ (p < 0.01 and p < 0.05, respectively), while decreasing the relative expression of PPARα (p < 0.0001), COX-2 (p < 0.0001) and IL-6 (p < 0.0001). In contrast, no effect was observed in the adhesion assay for either CLA.These results suggest that both CLAs at a low concentration have a neutral or modest anti-inflammatory effect in basal conditions, which may influence endothelial function and risk of vascular disease. Interestingly, at these low CLA concentrations some pro-inflammatory genes are upregulated while others are down regulated. This suggests complex effects of CLAs on inflammatory pathways.

scholarly journals Venom of the giant ant Dinoponera quadriceps attenuates inflammatory pain in mouse cutaneous wound healing model

2020 ◽  
Vol 42 ◽  
pp. e47680
Author(s):  
Ana Maria Sampaio Assreuy ◽  
Beatriz Lima Adjafre ◽  
Évilla Rayanne Oliveira Sousa ◽  
Ana Paula Negreiros Nunes Alves ◽  
Alana de Freitas Pires ◽  
...  
Keyword(s):  
Antinociceptive Effect ◽  
Folk Medicine ◽  
New Drugs ◽  
Northeastern Brazil ◽  
Mountain Range ◽  
Myeloperoxidase Activity ◽  
Mice Model ◽  
Bioactive Substances ◽  
Thermal Nociception ◽  
Cutaneous Wound

Arthropod venoms are potential sources of bioactive substances, providing tools for the validation of popular use and new drugs design. Ants belonging to the genus Dinoponera are used in the folk medicine to treat inflammatory conditions. It was previously demonstrated that the venom of the giant ant Dinoponera quadriceps (DqV), containing a mixture of polypeptides, elicit antinociceptive effect in mice models of chemical, mechanical and thermal nociception. The aim of this study was to evaluate DqV antiinflammatory and antihypernociceptive effects in a mice model of traumatic cutaneous wound. Colonies of D. quadriceps were collected in the ‘‘Serra de Maranguape’’ (State of Ceará, northeastern Brazil), a small mountain range located on the coastal zone, and the venom secreted by the ant glands was extracted with capillary tubes, further lyophilized and maintained at -20 ± 1ºC until use. Wounds were performed in the dorsum of Swiss mice. Animals received intravenous (i.v.) injection of DqV (50 µg -1kg day-1) during 3 days for evaluation of inflammatory parameters present in the wounds: hypernociception, leukocyte infiltrate, myeloperoxidase activity, nitrite nitrate-1 content. Data was tested by two-way ANOVA and Bonferroni’s post-hoc test. DqV reduced (2.7 folds) hypernociception at 48 hours, leukocyte infiltration by 65% at 6 hours and myeloperoxidase activity by 60% at 0.5 hour after wound induction. In conclusion, the venom extracted from D. quadriceps glands attenuates inflammation and hypernociception in mice cutaneous wounds.

Antinociceptive activities with the possible mechanisms of action of hydroethanol leaf extract of Eclipta prostrata Hassk (Astraceae) in mice

2021 ◽  
Vol 1 (1) ◽  
pp. 036-044
Author(s):  
Esther Oluwatoyin Agbaje ◽  
Odunayo New Year Ashimolowo
Keyword(s):  
Leaf Extract ◽  
Antinociceptive Effect ◽  
Cholinergic Receptor ◽  
Positive Control ◽  
Oral Route ◽  
Phytochemical Analysis ◽  
Eclipta Prostrata ◽  
Channel Opening ◽  
Pre Treatment ◽  
Analgesic Activities

Eclipta prostrata belongs to the family of plants known as Astraceae. It is a species of plant in the sunflower family, popularly known as "false daisy" in English. The whole plant contains the alkaloids nicotine and ecliptine; and used locally for treating bruises, cuts, wounds, liver disorders and pain, among others. This study investigated the analgesic activities of hydroethanolic leaf extract of Eclipta prostrata in rodents, using standard laboratory protocols. Doses of 50, 100, and 200 mg/kg were separately administered via oral route to the animals used in the various models - acetic acid-induced mouse writhing test, formalin - induced pain, hot plate-induced pain, and tail clip test. The effect of the extract was comparable to that produced by peripheral analgesics like aspirin and centrally acting analgesics like morphine used as positive control in the various models employed. The mechanism of action was elucidated through pre-treatment with naloxone (1 mg/kg), yohimbine (1mg/kg), ondasetron (0.2 mg/kg), atropine (2 mg/kg), glibenclamide (2 mg/kg). The extract activity was significant in all the models employed; however, it was more intense via the peripheral than the central pathway. Glibenclamide and atropine antagonized the activity of the decoction, which suggests that the herbal drug’s antinociceptive effect is mediated via potassium ion channel opening and cholinergic receptor. Phytochemical analysis of the leaf extract indicated the presence of flavonoids, phenols, alkaloids, phlobatanins, steroids, cardiac glycoside, and which probably contributed to the analgesic activity of the extract.

Anti-Inflammatory, Antipyretic and Analgesic Potential of Zilla spinosa in Animal Model

2019 ◽  
Author(s):  
Riaz Ullah ◽  
Abdelaaty A Shahat ◽  
Ali S Alqahtani ◽  
Omer Mohammed Almarfad ◽  
Mohammad Sayer M Alharbi ◽  
...  
Keyword(s):  
Analgesic Activity ◽  
Tail Flick ◽  
Chloroform Fraction ◽  
Test Model ◽  
Hot Plate Test ◽  
Tail Flick Test ◽  
Antipyretic Activity ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Analgesic Activities

The anti-inflammatory, antipyretic and analgesic activities of two concentrations (250 and 500 mg/kg) of the chloroform and butanol fractions of Zilla spinosa were determined. The carrageenan-induced rat paw edema assay was exercised for assessing the anti-inflammatory activity in rats, yeast-induced hyperthermia was utilized to assess the antipyretic activity in mice and the analgesic activity was measured by three different methods (hot-plate test model in mice, tail flick test in mice and acetic acid-induced writhing in mice). The antioxidant activity was studied by using the DPPH assay. The chloroform fraction of the methanol extract of Z. spinosa (ZSC) demonstrated the maximum inhibition of inflammation (50% at 500 mg/kg; 44% at 250 mg/kg). The chloroform fraction showed significant antipyretic activities (p andlt; 0.001 at 500 mg/kg) after 60 and 120 min of administration. ZSC also exhibited significant analgesic activity (p andlt;0.001). The butanol fraction (ZSB) was inactive in all the biological screening assays.

Methyl Gallate Inhibits the Production of Interleukin-6 and Nitric Oxide via Down-Regulation of Extracellular-Signal Regulated Protein Kinase in RAW 264.7 Cells

2010 ◽  
Vol 38 (05) ◽  
pp. 973-983 ◽  
Author(s):  
Hee-Sung Chae ◽  
Ok-Hwa Kang ◽  
Jang-Gi Choi ◽  
You-Chang Oh ◽  
Young-Seob Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Methyl Gallate ◽  
Extracellular Signal ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Oxide Synthase

To determine the anti-inflammatory and analgesic activities of methyl gallate (MG) isolated from Galla Rhois, MG was studied in vivo for its analgesic activities using the writhing response in mice. Anti-inflammatory activity of MG was evaluated for NO and IL-6 production in RAW 264.7 cells. MG inhibited LPS-induced NO and IL-6 production. Consistent with these observations, the protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were inhibited by MG. Moreover, MG suppressed the phosphorylation of ERK1/2 in LPS-induced RAW 264.7 cells in a dose-dependent manner. Taken together, the results of this study indicate that MG has anti-inflammatory effects.

Lactoferrin reduces colitis in rats via modulation of the immune system and correction of cytokine imbalance

2002 ◽  
Vol 283 (1) ◽  
pp. G187-G195 ◽  
Author(s):  
Jun-Ichi Togawa ◽  
Hajime Nagase ◽  
Katsuaki Tanaka ◽  
Masahiko Inamori ◽  
Tadashi Umezawa ◽  
...  
Keyword(s):  
Immune System ◽  
Distal Colon ◽  
Nuclear Factor Κb ◽  
Bovine Lactoferrin ◽  
Myeloperoxidase Activity ◽  
Intestinal Damage ◽  
Dependent Manner ◽  
Trinitrobenzenesulfonic Acid ◽  
Anti Inflammatory ◽  
Cytokine Imbalance

Natural immunomodulator lactoferrin is known to exert an anti-inflammatory effect. However, there have been no studies that examine the mode of action of lactoferrin in reducing intestinal damage. We investigated the effect of lactoferrin on a trinitrobenzenesulfonic acid (TNBS)-induced colitis model in rats. Bovine lactoferrin was given once daily through gavage, starting 3 days before (preventive mode) or just after TNBS administration (treatment mode) until death. The distal colon was removed to be examined. Colitis was attenuated by lactoferrin via both modes in a dose-dependent manner, as reflected by improvement in macroscopic and histological scores and myeloperoxidase activity. Lactoferrin caused significant induction of the anti-inflammatory cytokines interleukin (IL)-4 and IL-10, significant reductions in the proinflammatory cytokines tumor necrosis factor-α and IL-1β, and downregulation of the nuclear factor-κB pathway. We concluded that lactoferrin exerts a protective effect against colitis in rats via modulation of the immune system and correction of cytokine imbalance. Lactoferrin has potential as a new therapeutic agent for inflammatory bowel disease.

Evaluation of the Analgesic Effect of Aqueous Extract of Brugmansia suaveolens Flower in Mice: Possible Mechanism Involved

2010 ◽  
Vol 11 (4) ◽  
pp. 345-350 ◽  
Author(s):  
Ana Luiza Muccillo-Baisch ◽  
Alexander Garcia Parker ◽  
Gianni Peraza Cardoso ◽  
Marta Regina Cezar-Vaz ◽  
Maria Cristina Flores Soares
Keyword(s):  
Aqueous Extract ◽  
Benzodiazepine Receptors ◽  
Antinociceptive Effect ◽  
Dependent Manner ◽  
Hot Plate Test ◽  
Antinociceptive Effects ◽  
Brugmansia Suaveolens ◽  
The Central Nervous System ◽  
Dose Dependent ◽  
Abdominal Constriction

The study was conducted to test the aqueous extract of Brugmansia suaveolens (AEBs) flowers for their antinociceptive effects in mice. In the hot plate test, a significant increase in reaction time for two doses of AEBs at 60, 90, 120, and 150 min after treatment was noted. Pretreatment of animals with naloxone (5 mg/kg, intraperitoneally [IP]) left the antinociceptive effect of AEBs at a dose of 100 mg/kg unaffected at 60, 90, 120, and 150 min after treatment and at a dose of 300 mg/kg at 30 min but not at 90, 120, and 150 min. In the writhing test, the AEBs significantly inhibited acetic acid—induced abdominal constriction and was equally potent at both doses. Pretreatment with naloxone (5 mg/kg, IP) left the antinociceptive effect of both doses of AEBs unaffected. Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 20 mg/kg, IP) caused a significant change in the number of abdominal constrictions but did not change the antinociceptive effect of AEBs. Pretreatment of animals with methylene blue also did not change the effect of AEBs on the number of writhing movements in mice. Flumazenil (5 mg/kg, IP) antagonized the antinociceptive effects of diazepam and also reversed the antinociceptive effect of AEBs. AEBs showed a depressant effect on the central nervous system, and the treatment of mice with pentobarbital combined with AEBs increased the animals’ sleeping time in a dose-dependent manner. These results suggest that the antinociceptive activity of AEBs may be related in part to benzodiazepine receptors, although peripheral mechanisms cannot be excluded.

scholarly journals Topical Anti-Inflammatory and Analgesic Activities of Citrullus colocynthis Extract Cream in Rats

Medicina ◽  
2018 ◽  
Vol 54 (4) ◽  
pp. 51 ◽  
Author(s):  
Marzieh Pashmforosh ◽  
Hossein Rajabi Vardanjani ◽  
Hassan Rajabi Vardanjani ◽  
Mahdi Pashmforosh ◽  
Mohammad Khodayar
Keyword(s):  
Opioid Receptors ◽  
Formalin Test ◽  
Inflammatory Diseases ◽  
Antinociceptive Effect ◽  
Opioid Antagonist ◽  
Iranian Traditional Medicine ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Inflammatory Effect

Background and objectives:Citrullus colocynthis (CC), known as bitter apple, is used to treat diabetes in Iranian traditional medicine. The aim of this study is to evaluate the anti-inflammatory and analgesic activities of CC cream in rats. Materials and Methods: The carrageenan-induced edema in a rat hind paw was carried out to evaluate the topical anti-inflammatory effect of the CC fruit extract cream (2–8%) and the tissue levels of IL-6 and TNF-α were estimated by using a commercial ELISA kit. The topical antinociceptive activity of CC cream (2–8%) was evaluated in the rat formalin test. To determine the role of opioid receptors in the local antinociceptive effect of the CC cream, naloxone (20 μg/paw, i.pl.), a non-selective opioid antagonist, was used. Results: The results showed that the CC cream (2–8%) dose-dependently reduced the carrageenan-induced paw edema and reversed the changes in the level of TNF-α and IL-6 due to carrageenan-induced edema (p < 0.01). The anti-inflammatory effect of CC cream 8% was comparable to that of hydrocortisone ointment 1%. Furthermore, the application of CC cream (2–8%) dose-dependently inhibited both first and second phases of the formalin test (p < 0.05). The antinociceptive effect of the CC cream (8%) was comparable to that of methyl salicylate cream 30%. Moreover, the administration of naloxone significantly reversed the topical antinociceptive effect of the CC cream (p < 0.05). Conclusions: For the first time, this study indicated that the topical application of CC cream possesses significant anti-inflammatory and antinociceptive activities in animal models, which were probably mediated by opioid receptors and the suppression of pro-inflammatory cytokines (TNF-α and IL-6). Thus, the CC cream can be used to treat inflammatory pain and inflammatory diseases.

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