scholarly journals DISCONTINUOUS HYDRATION ON THE GERMINATION OF Mimosa caesalpiniifolia AND Pityrocarpa moniliformis SEEDS UNDER WATER STRESS

2020 ◽  
Vol 33 (2) ◽  
pp. 555-561
Author(s):  
JOSEFA PATRÍCIA BALDUINO NICOLAU ◽  
FRANCISCO EUDES DA SILVA ◽  
FRANCIVAL CARDOSO FELIX ◽  
SALVADOR BARROS TORRES ◽  
MAURO VASCONCELOS PACHECO ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Distilled Water ◽  
Initial Growth ◽  
Germination Time ◽  
Seedling Length ◽  
Severe Water Stress ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Osmotic Potentials

ABSTRACT The use of discontinuous hydration cycles can act in improving seed germination and seedling initial growth under water stress. In this context, the objective of this study was to evaluate the effect of discontinuous hydration on the germination of Mimosa caesalpiniifolia and Pityrocarpa moniliformis seeds under water stress. For this, seeds of these species were subjected to 0 (without hydration), 2 and 4 hydration cycles (18 h) and dehydration (12 and 18 h, respectively), with subsequent germination under water stress simulated for osmotic potentials of 0.0 (distilled water), -0.2, -0.4, -0.6 and -0.8 MPa. Seedlings were evaluated using tests of germination, germination speed index, mean germination time and seedling length. Discontinuous hydration in M. caesalpiniifolia and P. moniliformis seeds hampers germination and vigor of seedlings under water stress up to -0.6 MPa. However, the use of two cycles in P. moniliformis favors germination under more severe water stress (-0.8 MPa).

scholarly journals Does discontinuous hydration of Senna spectabilis (DC.) H.S. Irwin & Barneby var. excelsa (Schrad.) H.S. Irwin & Barneby (Fabaceae) seeds confer tolerance to water stress during seed germination?

2018 ◽  
Vol 40 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Ayslan Trindade Lima ◽  
Paulo Henrique de Jesus da Cunha ◽  
Bárbara França Dantas ◽  
Marcos Vinicius Meiado
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Stress Conditions ◽  
Hydration Time ◽  
Senna Spectabilis ◽  
Mean Germination Time ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials ◽  
Germination Parameters ◽  
Hydration And Dehydration

Abstract: Seed hydration memory is the ability of seeds to retain biochemical and physiological changes caused by discontinuous hydration. This study aimed to determine if Senna spectabilis (DC.) H.S. Irwin & Barneby var. excelsa (Schrad.) H.S.Irwin & Barneby (Fabaceae) present seed memory and evaluate the effects of hydration and dehydration cycles (HD) on the seed germination of this species when submitted to conditions of water stress. Seeds underwent HD cycles (0, 1, 2 and 3 cycles) corresponding to the hydration times X (6 hours), Y (16 hours) and Z (24 hours), determined from the imbibition curve, with 5 hours of dehydration and submitted to water stress conditions. Germination was evaluated at 0.0, -0.1, -0.3, -0.6 and -0.9 MPa, obtained with polyethylene glycol 6000 solution. Germinability (%), mean germination time (days) and hydrotime (MPa d-1) were calculated. The seeds of S. spectabilis var. excelsa are sensitive to the low osmotic potentials tested in this study, however, when submitted to the HD cycles of 16 hours hydration (time Y), the tolerance to water stress conditions is increased. In addition, the observed benefits on the evaluated germination parameters show that S. spectabilis var. excelsa present seed hydation memory.

scholarly journals Seed Germination and Initial Growth of Pepper Seedlings is Influenced by Environment Temperature and Priming Method

2020 ◽  
Vol 8 (4) ◽  
pp. 540
Author(s):  
Glenda Sallaku ◽  
Gjok Vuksani ◽  
Astrit Balliu
Keyword(s):  
Polyethylene Glycol ◽  
Germination Percentage ◽  
Early Growth ◽  
Distilled Water ◽  
Initial Growth ◽  
Environment Temperature ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Peg 8000 ◽  
Germination Parameters

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.

scholarly journals SEED GERMINATION OF Pouteria torta (MART.) RADLK. SUBESP. torta IN DIFFERENT ENVIRONMENTAL CONDITIONS

FLORESTA ◽  
2019 ◽  
Vol 50 (1) ◽  
pp. 923
Author(s):  
Ademir Kleber Morbeck Oliveira ◽  
Jose Carlos Pina
Keyword(s):  
Seed Germination ◽  
Native Species ◽  
Primary Root ◽  
Seed Vigor ◽  
Limited Information ◽  
Germination Time ◽  
Randomized Design ◽  
Germination Speed ◽  
Different Temperatures ◽  
Mean Germination Time

Germination is considered a critical step in the seedling production process aiming the recovery of environments. Thus, understanding the germination behavior of native species with potential for economic use contributes to the development of the forest seed and seedling chain. Considering the limited information on the tree species Pouteria torta subesp. torta, this study aimed to evaluate its seed germination and formation of normal seedlings using different temperatures and substrates. The experiment was conducted in germination chamber in a research laboratory using two substrates (paper and vermiculite) and six temperatures (20, 25, 30, 35, 20-30 and 25-35 °C) in a completely randomized design in a 2x6 factorial arrangement. Daily evaluation was conducted to verify primary root emission, seed vigor, and seedling formation. The results indicated that the primary root protrusion process began after 20 days of testing under the best conditions, with germination time lasting longer than two months. The results obtained also showed that there was interaction between factors, with use of the vermiculite substrate and the temperatures of 35 and 25-35 °C producing the best germination rates and germination speed index, and use of the paper substrate and the temperatures of 30 and 35 °C showing the best mean germination time. Considering the analyzed variables (germination, germination speed index, and formation of normal seedlings), the temperatures of 35 and 25-35 °C and the vermiculite substrate are the most suitable for the production of Pouteria torta subesp. torta seedlings.

scholarly journals Effects of drought stress on germination and seedling growth of different field pea varieties

2021 ◽  
pp. 59-70
Author(s):  
Gordana Petrovic ◽  
Tomislav Zivanovic ◽  
Radmila Stikic ◽  
Zorica Nikolic ◽  
Dusica Jovicic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Osmotic Stress ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Field Pea ◽  
Tolerance Index ◽  
Germination Time ◽  
Mean Germination Time ◽  
Effects Of Drought ◽  
Osmotic Potentials

Germination and seedling growth of seven genotypes of field pea (Pisum sativum L.) were studied in PEG-6000 solution having osmotic potentials -0.1 and -0.2 MPa. A study was undertaken to evaluate the influence of different osmotic potentials (MPa) on seed germination percentage (GP) and mean germination time (MGT). Results show that the percentage of germination decreased with a decrease in osmotic potential, while mean germination time increased. Variety Javor is much more sensitive than the other varieties at all levels of osmotic stress. By contrast, the lowest sensitivity of germination and MGT was found in varieties Mraz and Trezor. Seed germination tests at -0.1 to -0.2 MPa have the potential to be used as tests in field pea. Osmotic stress exposure and its duration significantly affected the growth of seedlings (shoot and root) and the accumulation of biomass, while its effect was more prominent on the growth of the shoot than on root growth, which was also confirmed by the root tolerance index.

scholarly journals Seed Germination and Initial Growth of Quinoa Seedlings Under Water and Salt Stress

2019 ◽  
Vol 11 (15) ◽  
pp. 153 ◽  
Author(s):  
Geovana Facco Barbieri ◽  
Raquel Stefanello ◽  
Janine Farias Menegaes ◽  
Janete Denardi Munareto ◽  
Ubirajara Russi Nunes
Keyword(s):  
Seed Germination ◽  
Osmotic Potential ◽  
Chenopodium Quinoa ◽  
Dry Mass ◽  
Germination Percentage ◽  
Initial Growth ◽  
Peg 6000 ◽  
Germination Process ◽  
Seedling Length ◽  
Osmotic Potentials

Excessive amounts of salts and soil water deficiency interfere on seed germination and the full development of several crops. The objective of this research was to evaluate the effect of water stress and salinity on the germination process and initial growth of quinoa (Chenopodium quinoa Willd.) seedlings. In the first experiment, two quinoa seed lots with different physiological conditions were distributed on paper soaked in aqueous solution containing polyethylene glycol PEG-6000 in osmotic potentials corresponding to 0.0; -0.1; -0.2; -0.3 and -0.4 MPa and held at 20 °C under 8 hours of light exposition. In the second experiment, solutions of sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2) and magnesium chloride (MgCl2) were used to simulate the effect of salinity using the osmotic potentials, temperature and light conditions previously described. Assessed parameters were the germination percentage, first count, length and dry mass of seedlings. There was a reduction in quinoa germination percentage, first seed count and seedling length as the osmotic potential decreased in CaCl2, NaCl, KCl, MgCl2 and PEG-6000 solutions. The quinoa seeds exhibited higher tolerance to NaCl and KCl salts in the germination process and initial seedling growth. The progressive reduction of the osmotic potential induced by salts NaCl, KCl, CaCl2, MgCl2 and PEG-6000 negatively affects seed germination and initial growth of quinoa seedlings.

Seed-germination responses of Calotropis procera (Asclepiadaceae) to temperature and water stress in northern Australia

2016 ◽  
Vol 64 (5) ◽  
pp. 441 ◽  
Author(s):  
E. O. Menge ◽  
S. M. Bellairs ◽  
M. J. Lawes
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Seedling Survival ◽  
Seedling Emergence ◽  
Seed Mass ◽  
Calotropis Procera ◽  
Northern Australia ◽  
Germination Time ◽  
Seed Biology ◽  
Mean Germination Time

Understanding the seed biology of the introduced weed rubber bush (Calotropis procera (Aiton, W.T.Aiton)) is critical to its management in northern Australia. We examined the numbers of seeds produced and the effects of environmental temperature and water stress on germination performance (germinability G; mean germination time MGT) of rubber bush seeds from across northern Australia. Germination trials were conducted using seeds from wild populations monitored for 3 years. Seed numbers per fruit did not vary significantly among the six populations studied (mean ± s.e. = 433.2 ± 19.0), but seed mass did (range from 8.32 ± 0.24 to 5.24 ± 0.06 mg), with no negative correlation between the measures. Maximum seed germination (68–100%) occurred at 30°C, associated with a mean germination time of 2.58 days. Under water stress, the proportion of germinated seeds declined significantly with increasing temperature from 92.5 ± 1.1% at 20°C and 0 MPa to 2.8 ± 1.7% at 40°C and –0.4 MPa respectively. Seeds were unable to germinate at ambient temperatures ≥40°C, but remained quiescent and hence viable. Planting depth influenced seedling emergence, with minimal germination of seeds on the surface (5.8%) but 88.5% germination at 3-cm depth. The effect of water stress was dependent on temperature, with water stress inducing a reduction in optimum germination temperature from 30°C to 20°C. Phenotypic plasticity in G and MGT did not show clear patterns among populations or years. Short MGTs increase seedling survival by rapid transition from endosperm resources to photosynthesis, whereas seed quiescence (cf. dormancy) optimises germination opportunities in a semiarid environment. Thus, the germination traits reported in the present study are likely to promote seedling survival and potential spread of rubber bush in semiarid Australia.

scholarly journals Physiological potential of stylosanthes cv. Campo Grande seeds coated with different materials

2015 ◽  
Vol 37 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Priscilla Brites Xavier ◽  
Henrique Duarte Vieira ◽  
Cynthia Pires Guimarães
Keyword(s):  
Activated Carbon ◽  
Calcium Silicate ◽  
Dry Matter ◽  
Seed Quality ◽  
Maximum Diameter ◽  
Minimum Diameter ◽  
Germination Time ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Limestone Sand

The aim of this study was to assess the effect of different coatings on the physiological potential of stylosanthes cv. Campo Grande seeds. The treatments were: uncoated seeds; limestone + PVA glue; limestone + sand + PVA glue; limestone + activated carbon + PVA glue; calcium silicate + PVA glue; calcium silicate + sand + PVA glue; calcium silicate + activated carbon + PVA glue. Posteriorly, the seeds were analyzed for water content (WC), maximum diameter (MAD) and minimum diameter (MID), thousand seed weight (TSW), germination test, germination speed index (GSI), mean germination time (MGT), emergence, emergence speed index (ESI), mean emergence time (MET), shoot and root length, fresh and dry matter of shoot and root. The coating increased the TSW, MAD and MID and decreased its WC. The treatments comprising limestone + PVA glue and limestone + sand + PVA glue increased the germination time, but none of the treatments negatively affected the physiological seed quality. Treatment with calcium silicate + PVA glue was outstanding for germination speed index and fresh and dry matter of shoot and root in the stylosanthes cv. Campo Grande seeds coating.

scholarly journals Can Bulk and Nanosized Titanium Dioxide Particles Improve Seed Germination Features of Wheatgrass (Agropyron desertorum)

2013 ◽  
Vol 5 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Reyhaneh AZIMI ◽  
Hassan FEIZI ◽  
Mohammad KHAJEH HOSSEINI
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Germination Time ◽  
Positive Effects ◽  
Tio2 Particles ◽  
Nanosized Titanium Dioxide ◽  
Mean Germination Time ◽  
Germination Value ◽  
Improve Seed Germination ◽  
Nanosized Tio2

The goal of this study was to evaluate concentrations of nanosized TiO2 at 0, 5, 20, 40, 60 and 80 mg L-1 with bulk TiO2 for possible stimulatory effects on wheatgrass seed germination and early growth stage. After 14 days of seed incubation, germination percentage improved by 9% following exposure to 5 ppm nanosized TiO2 treatment comparing to control. Similar positive effects occurred in terms of germination value and mean daily germination. Application of bulk TiO2 particles in 80 ppm concentration greatly decreased the majority of studied traits. Therefore phytotoxicity effect observed on wheatgrass seedling by application of bulk TiO2 particles in 80 ppm concentration. Exposure of wheatgrass seeds to 5 ppm nanosized TiO2 and bulk and nanosized TiO2 at 60 ppm obtained the lowest mean germination time but higher concentrations did not improve mean germination time. In general, there was a positive response by wheatgrass seed to some concentrations of nanosized TiO2. Usage of nanoparicles in order to improve germination and establishment of range plant in adverse environments similar to rangeland could be possible.

scholarly journals Response of Lupinus bracteolaris seeds to pre-germinative treatments and experimental conditions

2021 ◽  
Vol 12 (2) ◽  
pp. 51-66
Author(s):  
Luciana Pinto Paim ◽  
Eduarda Demari Avrella ◽  
Juliana Carolina Alves Horlle ◽  
Claudimar Sidnei Fior ◽  
Marília Lazarotto ◽  
...  
Keyword(s):  
Seed Germination ◽  
Substrate Temperature ◽  
Water Intake ◽  
White Light ◽  
Green Light ◽  
Experimental Conditions ◽  
Germination Time ◽  
Randomized Design ◽  
Germination Speed ◽  
Completely Randomized Design

Contextualization: Lupinus bracteolaris is a heliophite species that vegetates low lands, open slopes and sandstone hills. Knowledge gap: Despite the high rusticity and potential for covering poor soils, there is still no information to assist in the analysis of its seeds. Purpose: To determine pre-germinative treatments, substrate, temperature, luminosity and water intake in the vigor of Lupinus bracteolaris seeds. Methodology: Fruits were collected from 20 matrix plants, then the seeds were processed and submitted to the following tests: pre-germinative methods (six treatments at 25°C); substrates and temperatures (sand and paper at 20, 25 and 30°C); photoblast test (white light, diffuse-green light and continuous dark under 20°C) and the imbibition curve (control and scarification between sandpaper under 20°C). Evaluations: percentage of germination and formed seedlings, germination speed index, average germination time and seedling formation, using a completely randomized design. Results and conclusions: According to the analyzed variables, the results showed superiority for the pre-germinative treatment between sandpapers for 20 seconds. In addition, the substrate paper germibox and the temperature of 20°C were the most suitable during seed germination. The seeds of Lupinus bracteolaris were classified as neutral-photoblastic and without dormancy.

scholarly journals EFFECTS OF TEMPERATURE, SUBSTRATE AND LUMINOSITY CONDITIONS ON CHIA SEED GERMINATION

2019 ◽  
Vol 32 (2) ◽  
pp. 411-418
Author(s):  
CLAUDIA KELY PIRES DE MATTOS SORANA ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
FERNANDA BRITO CARDOSO ◽  
TIAGO ROQUE BENETOLI DA SILVA ◽  
ANA CARINA DA SILVA CÂNDIDO ◽  
...  
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Germination Test ◽  
Paper Substrate ◽  
Randomized Design ◽  
Chia Seed ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Effects Of Temperature ◽  
Completely Randomized Design

ABSTRACT The aim of the present study was to evaluate the effects of temperature, substrate, and luminosity on chia seeds in order to determine adequate conditions for a germination test. The experiment consisted of a completely randomized design, in a 4 × 5 × 2 factorial scheme (four substrates × five temperatures × two luminosity conditions), with four replications. The following variables were analyzed: first germination count, germination, germination speed index, and mean germination time. Germination was best at 15, 20, 25, and 30 °C for the on-paper, paper roll, and sand substrates. However, in the presence of luminosity and at 25 °C, the on-paper substrate had the highest mean. For all substrates, the lowest germination was achieved at 35 °C, proving that high temperatures reduce chia seed germination. Germination speed index was highest at 25 and 30 °C for on-paper, paper roll, and sand substrates, in both the presence and absence of luminosity. Chia seeds are considered to be neutral photoblasts. Germination tests should be performed with the on-paper substrate, at 25 °C, and with a duration of seven days. The first count should be carried out four days after establishment of the test.

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