scholarly journals Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis

1997 ◽  
Vol 39 (6) ◽  
pp. 313-318 ◽  
Author(s):  
Julia Maria COSTA-CRUZ ◽  
Carina Barbosa BULLAMAH ◽  
Maria do Rosário F. GONÇALVES-PIRES ◽  
Dulcinéa Maria B. CAMPOS ◽  
Miguel Alípio VIEIRA
Keyword(s):  
Indirect Immunofluorescence ◽  
Linear Correlation ◽  
Mass Production ◽  
Low Cost ◽  
Strongyloides Stercoralis ◽  
Human Igg ◽  
Significant Linear Correlation ◽  
The Ideal

Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p <FONT FACE="Symbol">£</font> 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended

Should CD138 Immunohistochemistry be Standard Recommended Practice for Bone Marrow Evaluation of Plasma Cell Neoplasms?

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S110-S110
Author(s):  
A Vijayanarayanan ◽  
K Inamdar ◽  
M Menon ◽  
P Kuriakose
Keyword(s):  
Bone Marrow ◽  
Plasma Cell ◽  
Linear Correlation ◽  
Plasma Cells ◽  
Pearson Correlation ◽  
Protein Electrophoresis ◽  
Pearson Correlation Coefficient ◽  
Cell Percentage ◽  
Significant Linear Correlation ◽  
Plasma Cell Neoplasms

Abstract Introduction/Objective Myeloma diagnosis by a pathologist requires 10% plasma cells (PC) or a biopsy proven plasmacytoma in addition to myeloma defining events. PC% &gt; 60% is a biomarker of malignancy under this definition. WHO allows for assesment of plasma cell percentage either by aspirate count or by CD138 immunohistochemistry (IHC). There is lack of consensus on aspirate smear adequacy for PC% estimation. Uneven distribution of plasma cells, hemodilution and/or patchy infiltration can lead to gross underestimation. We compared PC% by aspirate count and CD138 IHC and established corelation with serum protein electrophoresis (SPEP) values. Methods 67 myeloma cases were included after excluding cases with suboptimal or inadequate aspirate smears. Two hematopathologists evaluated the diagnostic marrow (therapy naive) for PC% by aspirate count and CD138 IHC on biopsy/clot section. Corresponding SPEP and Free light chain (FLC) values were obtained. Correlation coefficent was calculated using Pearson correlation coefficient (GraphPad Prism). Results The Ig subtypes included IgG (41/67) and IgA (17/67). 12 cases had available FLC values. Both average and median PC% by CD138 IHC was considerably higher (50%, 52%) compared to aspirate count (29%, 21%). However, PC% by aspirate smear count and CD138 IHC demonstrated a significant linear correlation (r=0.71, p60% by CD138 (and not by aspirate count). Conclusion CD138 IHC based PC% is consistently higher, nevertheless, statistically significant linear corelation is observed between aspirate count PC% and CD138 IHC. A significant linear correlation is observed between CD138 IHC and SPEP (IgG and IgA), however, no such correlation is observed with aspirate count. More cases were diagnosed as myeloma (11%) and higher propotion of cases (35%) had biomarker of malignancy i.e. PC% &gt;60% by CD138 IHC. Based on these findings, we propose estimation of PC% by CD138 immunostain be a recommended standard practice for better clinicopathologic and biologic correlation.

scholarly journals Whispering Gallery Mode Based Optical Fiber Sensor for Measuring Concentration of Salt Solution

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Chia-Chin Chiang ◽  
Jian-Cin Chao
Keyword(s):  
Optical Fiber ◽  
Mass Production ◽  
Low Cost ◽  
Optical Fiber Sensor ◽  
Whispering Gallery Mode ◽  
Solution Concentration ◽  
Fiber Sensor ◽  
Average Sensitivity ◽  
Whispering Gallery ◽  
The Right

An optical fiber solution-concentration sensor based on whispering gallery mode (WGM) is proposed in this paper. The WGM solution-concentration sensors were used to measure salt solutions, in which the concentrations ranged from 1% to 25% and the wavelength drifted from the left to the right. The experimental results showed an average sensitivity of approximately 0.372 nm/% and anR2linearity of 0.8835. The proposed WGM sensors are of low cost, feasible for mass production, and durable for solution-concentration sensing.

Buried seed populations in the montane, subalpine, and alpine belts of Mont Jacques-Cartier, Quebec

1988 ◽  
Vol 66 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Hubert Morin ◽  
Serge Payette
Keyword(s):  
Seed Bank ◽  
Linear Correlation ◽  
Altitudinal Gradient ◽  
Number Of Species ◽  
Significant Linear Correlation ◽  
Seed Population ◽  
Close Relationship ◽  
Buried Seed ◽  
Study Sites ◽  
Number Of Seeds

The analysis of the total buried seed population along an altitudinal gradient in the Mont Jacques-Cartier area, southern Quebec, reveals a close relationship between buried seed flora and aboveground vegetation. Eighty-one percent of the species present in the total buried seed population were present in the aboveground vegetation of the study sites. The total number of seeds in the buried seed population, the number of species in the total buried seed population, and the number of seeds and species in the seed bank did not show any significant linear correlation with altitude. A shift from a boreal to an arctic–alpine buried seed population with increasing altitude was observed, instead of a decrease in seed bank size.

scholarly journals Domiciliary Cockroaches as Carriers of Human Intestinal Parasites in Lagos Metropolis, Southwest Nigeria: Implications for Public Health

2018 ◽  
pp. 141-151 ◽  
Author(s):  
Adedotun A Adenusi ◽  
Mary I Akinyemi ◽  
Dele Akinsanya
Keyword(s):  
Public Health ◽  
Disease Transmission ◽  
Periplaneta Americana ◽  
Intestinal Parasites ◽  
Low Cost ◽  
Parasite Prevalence ◽  
Strongyloides Stercoralis ◽  
Housing Type ◽  
Public Health Importance ◽  
Southwest Nigeria

Background: Domiciliary cockroaches are obnoxious pests of significant medical importance. We investigated the prevalence of human intestinal parasites in cockroaches and its attendant public health importance. Methods: Overall, 749 cockroaches (Periplaneta americana, 509, Blattella germanica, 240) caught by trapping from 120 households comprising 3 different housing types in Somolu, Lagos metropolis, southwest Nigeria, in 2015 were screened for human intestinal parasites using standard parasitological techniques. Results: The prevalence of human intestinal parasites in cockroaches was 96.4%. There was no statistically signifi­cant difference (P> 0.05) in parasite prevalences between P. americana (95.7%) and B. germanica (97.9%). Parasite species identified and their prevalence were as follows: Entamoeba histolytica/dispar (44.1%), E. coli (37.8%), Gi­ardia lamblia (18.7%), Cryptosporidium sp. (13.8%), Ascaris lumbricoides (61.3%), Trichuris trichiura (55.8%), hookworms (11.6%), Strongyloides stercoralis (11.7%), Taenia/Echinococcus spp. (10.5%), Enterobius vermicularis (17.2%) and Hymenolepis nana (11.6%). Parasite prevalence and burdens varied with housing type; the prevalence was significantly higher statistically (P< 0.05) in cockroaches from low-cost bungalow, LCB (100%) and low-cost, 2-storey, LC2-S (100%) houses than in medium-cost flats, MCF (81.3%). Parasite burdens were also significantly higher statistically (P< 0.05) in cockroaches from LCB or LC2-S than in cockroaches from MCF. Parasite preva­lences between cockroach gut and body surfaces were not statistically significant (P> 0.05) but mean parasite bur­dens in gut were significantly higher statistically (P< 0.05) than on body surfaces. Conclusion: Cockroaches types carry transmissive stages of human intestinal parasites and may act as reservoirs and potential mechanical vectors for disease transmission.

scholarly journals RELATIONSHIP BETWEEN HIGHER-ORDER THINKING SKILLS (HOTS) AND ENGLISH ACHIEVEMENT

Prominent ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 45-53
Author(s):  
Ernis Sholikah ◽  
Suprihadi Suprihadi ◽  
Nuraeningsih Nuraeningsih
Keyword(s):  
Key Words ◽  
Survey Research ◽  
Linear Correlation ◽  
Higher Order Thinking ◽  
Thinking Skills ◽  
Higher Order ◽  
Higher Order Thinking Skills ◽  
Significant Linear Correlation ◽  
English Achievement ◽  
Level Of Significance

Higher-order thinking skills (HOTS) is a new concept of thinking in Curriculum 2013 in Indonesia, which is based on the revised top three levels in Bloom’s Taxonomy: analyzing, evaluating, and creating. This survey research used 44 students who were purposively sampled. The objective of this research was to determine if there was a linear correlation between HOTS and the English achievement of the eleventh grade students of SMA N 2 Kudus. Using Pearson’s Product Moment Correlation in the level of significance .05 and degree of freedom 42, this research showed that there was a high positive significant linear correlation between HOTS and the English achievement of the eleventh grade students of SMA N 2 Kudus. Key words: HOTS, taxonomy, curriculum 2013, English achievement

Lithography Process Challenges for 3D and 2.5D Applications

2013 ◽  
Vol 2013 (DPC) ◽  
pp. 000398-000424
Author(s):  
Doug Shelton ◽  
Tomii Kume
Keyword(s):  
High Resolution ◽  
Process Optimization ◽  
Mass Production ◽  
Low Cost ◽  
High Density ◽  
Through Silicon Via ◽  
Key Technology ◽  
Lithography Process ◽  
Advanced Packaging

Lithography process optimization is a key technology enabling mass production of high-density interconnects using 3D and 2.5D technologies. In this paper, Canon will continue its discussion of lithography optimization of thick-resist profiles and overlay accuracy to increase process margins for Through-Silicon Via (TSV) and Redistribution Layer (RDL) applications. Canon will also provide updates on the FPA-5510iV and FPA-5510iZ i-line steppers that are gaining acceptance as high-resolution, and low-cost lithography solutions for aggressive advanced packaging, 3D and 2.5D applications.

Lithography Process Optimization for 3D and 2.5D Applications

2013 ◽  
Vol 2013 (1) ◽  
pp. 000790-000793 ◽  
Author(s):  
Doug Shelton ◽  
Tomii Kume
Keyword(s):  
High Resolution ◽  
Process Optimization ◽  
Preliminary Data ◽  
Mass Production ◽  
Low Cost ◽  
High Density ◽  
Through Silicon Via ◽  
Key Technology ◽  
Lithography Process ◽  
Advanced Packaging

Lithography process optimization is a key technology enabling mass production of high-density interconnects using 3D and 2.5D technologies. In this paper, Canon continues its investigation of lithography optimization of thick-resist profiles and overlay accuracy to increase process margins for Through-Silicon Via (TSV) and Redistribution Layer (RDL) applications. Canon will also provide updates on the FPA-5510iV and FPA-5510iZ i-line steppers that are gaining acceptance as high-resolution, and low-cost lithography solutions for aggressive advanced packaging, 3D and 2.5D applications also preliminary data illustrating 450 mm wafer process challenges.

scholarly journals Fides Testi’s Use of the Airbrush in Italian Art Textiles in the 1930s

Arts ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 96
Author(s):  
Lucia Mannini
Keyword(s):  
United States ◽  
Mass Production ◽  
Essential Element ◽  
The United States ◽  
Geometric Shapes ◽  
Textile Design ◽  
The Creation ◽  
Italian Art ◽  
The Ideal

The Modernist aesthetic, which spread all over Europe and in the United States in the 1920s and 1930s, found the airbrush decorating technique to be the ideal instrument for expressing the requirements for an extreme synthesis of form. This was considered an essential element of the style, thanks to the areas of uniform color that shaded lighter tones inside basic, often geometric, shapes. The airbrush was used in that period mainly for graphics and for decorating ceramics, but it was also employed in other fields such as textile design. In Italy, the airbrush technique became popular in various artistic sectors including textiles, both for mass production and in the creation of single artistic pieces and in this latter field, Fides Testi was a leading figure.

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