Polygala extraaxillaris: oxidative stress in Brachiaria decumbens mediated by volatile oils

The volatile oils extracted from the roots of Polygala extraaxillaris were analyzed to assess whether they increase oxidative stress in Brachiaria decumbens var. Piatã, as well as to assess their effect on cellular division and cytotoxicity in laboratory. Six concentrations were used (0%, 0.35%, 0.65%, 1.25%, 0.65%, and 5.0%) with four repetitions of 25 seeds. The substance 1-(2-hydroxyphenyl) - ethanone was identified as the major constituent of the volatile oils. The results showed that the highest concentrations of the oils resulted in an increase in the oxidative stress in B. decumbens, as well as alteration in germination and growth, with a consequent reduction in the process of cellular division, causing changes in the growth standard and antioxidant defense.

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Are insulin-resistance and oxidative stress cause or consequence of aging

Experimental Biology and Medicine ◽

10.1177/1535370220929621 ◽

2020 ◽

Vol 245 (14) ◽

pp. 1260-1267

Author(s):

Sylwia Dzięgielewska-Gęsiak ◽

Dorota Stołtny ◽

Alicja Brożek ◽

Małgorzata Muc-Wierzgoń ◽

Ewa Wysocka

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Antioxidant Status ◽

Antioxidant Defense ◽

Total Antioxidant Status ◽

Thiobarbituric Acid ◽

Elderly Individuals ◽

Total Antioxidant ◽

Antioxidant Defense Systems ◽

Antioxidant Markers

Insulin resistance (IR) may be associated with oxidative stress and leads to cardiovascular disorders. Current research focuses on interplay between insulin-resistance indices and oxidant-antioxidant markers in elderly individuals with or without insulin-resistance. The assessment involved anthropometric data (weight, height, BMI, percentage of body fat (FAT)) and biochemical tests (glucose, lipids, serum insulin and plasma oxidant-antioxidant markers: Thiobarbituric Acid-Reacting Substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1) and total antioxidant status). Insulin resistance index (IR) assuming a cut-off point of 0.3 allows to divides groups into: insulin sensitive group (InsS) IR < 0,3 ( n = 35, median age 69.0 years) and insulin-resistant group (InsR) IR ≥ 0.3 ( n = 51, median age 71.0 years). Lipids and antioxidant defense system markers did not differentiate the investigated groups. In the InsR elderly group, the FAT was increased ( P < 0.000003) and TBARS ( P = 0.008) concentration decreased in comparison with InsS group. A positive correlation for SOD-1 and total antioxidant status ( P < 0.05; r = 0.434) and a negative correlation for TBARS and age ( P < 0.05 with r = −0.421) were calculated in InsR individuals. In elderly individuals, oxidative stress persists irrespective of insulin-resistance status. We suggest that increased oxidative stress may be consequence of old age. An insulin action identifies those at high risk for atherosclerosis, via congruent associations with oxidative stress and extra- and intra-cellular antioxidant defense systems. Thus, we maintain that insulin-resistance is not the cause of aging. Impact statement Insulin resistance is associated with oxidative stress leading to cardiovascular diseases. However, little research has been performed examining elderly individuals with or without insulin-resistance. We demonstrate that antioxidant defense systems alone is not able to abrogate insulin action in elderly individuals at high risk for atherosclerosis, whereas the combined oxidant-antioxidant markers (thiobarbituric acid-reacting substances (TBARS), Cu,Zn-superoxide dismutase (SOD-1), and total antioxidant status (TAS)) might be more efficient and perhaps produce better clinical outcome. In fact, a decrease in oxidative stress and strong interaction between antioxidant defense can be seen only among insulin-resistant elderly individuals. This is, in our opinion, valuable information for clinicians, since insulin-resistance is considered strong cardiovascular risk factor.

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Skeletal muscle uncoupling-induced longevity in mice is linked to increased substrate metabolism and induction of the endogenous antioxidant defense system

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00518.2012 ◽

2013 ◽

Vol 304 (5) ◽

pp. E495-E506 ◽

Cited By ~ 26

Author(s):

S. Keipert ◽

M. Ost ◽

A. Chadt ◽

A. Voigt ◽

V. Ayala ◽

...

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Mapk Signaling ◽

Redox Signaling ◽

Defense System ◽

High Fat ◽

Substrate Metabolism ◽

Endogenous Antioxidant

Ectopic expression of uncoupling protein 1 (UCP1) in skeletal muscle (SM) mitochondria increases lifespan considerably in high-fat diet-fed UCP1 Tg mice compared with wild types (WT). To clarify the underlying mechanisms, we investigated substrate metabolism as well as oxidative stress damage and antioxidant defense in SM of low-fat- and high-fat-fed mice. Tg mice showed an increased protein expression of phosphorylated AMP-activated protein kinase, markers of lipid turnover (p-ACC, FAT/CD36), and an increased SM ex vivo fatty acid oxidation. Surprisingly, UCP1 Tg mice showed elevated lipid peroxidative protein modifications with no changes in glycoxidation or direct protein oxidation. This was paralleled by an induction of catalase and superoxide dismutase activity, an increased redox signaling (MAPK signaling pathway), and increased expression of stress-protective heat shock protein 25. We conclude that increased skeletal muscle mitochondrial uncoupling in vivo does not reduce the oxidative stress status in the muscle cell. Moreover, it increases lipid metabolism and reactive lipid-derived carbonyls. This stress induction in turn increases the endogenous antioxidant defense system and redox signaling. Altogether, our data argue for an adaptive role of reactive species as essential signaling molecules for health and longevity.

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Impact of seasonally frozen soil on germinability and antioxidant enzyme activity of Picea asperata seeds

Canadian Journal of Forest Research ◽

10.1139/x09-001 ◽

2009 ◽

Vol 39 (4) ◽

pp. 723-730 ◽

Cited By ~ 3

Author(s):

Jihong Qin ◽

Qing Liu

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Germination Rate ◽

Antioxidant Defense System ◽

Antioxidant Enzyme Activity ◽

Frozen Soil ◽

Defense System ◽

Frozen Ground ◽

Subalpine Zone ◽

Picea Asperata

In the subalpine zone of the Qinghai–Tibetan Plateau of China, Dragon spruce (Picea asperata Mast.) is commonly used for reforestation. The aim of the present work was to study the effects of seasonally frozen soil on the germination of P. asperata seeds and to investigate whether these effects were associated with resumption of the antioxidant defense system. The nonfrozen treatment resulted in near failure of germination (1%) and was associated with relatively high levels of hydrogen peroxide (H2O2) and low activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). Germination of P. asperata seeds at 10 cm under the seasonally frozen soil was higher than that at 5 cm by 26%; this higher germination rate was associated with the recovery of SOD, CAT, and APX activities. The levels of malondialdehyde (MDA) in seeds from seasonally frozen treatments were higher than those in the nonfrozen treatment, implying greater lipid peroxidation and that frozen seeds might have suffered from oxidative stress. The results indicate that seasonally frozen soil facilitated the germination of P. asperata seeds and that germination was closely related to the resumption of antioxidant enzymes activity. Overall, these findings suggest that the disappearance of seasonally frozen ground caused by global warming might result in failure of regeneration of P. asperata.

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Self-Report of Fruit and Vegetable Intake that Meets the 5 A Day Recommendation Is Associated with Reduced Levels of Oxidative Stress Biomarkers and Increased Levels of Antioxidant Defense in Premenopausal Women

Journal of the Academy of Nutrition and Dietetics ◽

10.1016/j.jand.2013.01.019 ◽

2013 ◽

Vol 113 (6) ◽

pp. 776-785 ◽

Cited By ~ 23

Author(s):

Stephanie M. Rink ◽

Pauline Mendola ◽

Sunni L. Mumford ◽

Jill K. Poudrier ◽

Richard W. Browne ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Vegetable Intake ◽

Premenopausal Women ◽

Fruit And Vegetable Intake ◽

Self Report ◽

Fruit And Vegetable ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers

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Impact of CuO nanoparticles on maize: Comparison with CuO bulk particles with special reference to oxidative stress damages and antioxidant defense status

Chemosphere ◽

10.1016/j.chemosphere.2021.131911 ◽

2022 ◽

Vol 287 ◽

pp. 131911

Author(s):

Doyel Roy ◽

Sinchan Adhikari ◽

Ayan Adhikari ◽

Supriya Ghosh ◽

Ikbal Azahar ◽

...

Keyword(s):

Oxidative Stress ◽

Special Reference ◽

Antioxidant Defense ◽

Cuo Nanoparticles

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Identification of Redox-Sensitive Transcription Factors as Markers of Malignant Pleural Mesothelioma

Cancers ◽

10.3390/cancers13051138 ◽

2021 ◽

Vol 13 (5) ◽

pp. 1138

Author(s):

Martina Schiavello ◽

Elena Gazzano ◽

Loredana Bergandi ◽

Francesca Silvagno ◽

Roberta Libener ◽

...

Keyword(s):

Oxidative Stress ◽

Transcription Factors ◽

Malignant Pleural Mesothelioma ◽

Antioxidant Defense ◽

Asbestos Exposure ◽

Predictive Biomarkers ◽

Antioxidant Systems ◽

Pleural Mesothelioma ◽

Related Factor ◽

Aggressive Cancer

Although asbestos has been banned in most countries around the world, malignant pleural mesothelioma (MPM) is a current problem. MPM is an aggressive tumor with a poor prognosis, so it is crucial to identify new markers in the preventive field. Asbestos exposure induces oxidative stress and its carcinogenesis has been linked to a strong oxidative damage, event counteracted by antioxidant systems at the pulmonary level. The present study has been focused on some redox-sensitive transcription factors that regulate cellular antioxidant defense and are overexpressed in many tumors, such as Nrf2 (Nuclear factor erythroid 2-related factor 2), Ref-1 (Redox effector factor 1), and FOXM1 (Forkhead box protein M1). The research was performed in human mesothelial and MPM cells. Our results have clearly demonstrated an overexpression of Nrf2, Ref-1, and FOXM1 in mesothelioma towards mesothelium, and a consequent activation of downstream genes controlled by these factors, which in turn regulates antioxidant defense. This event is mediated by oxidative free radicals produced when mesothelial cells are exposed to asbestos fibers. We observed an increased expression of Nrf2, Ref-1, and FOXM1 towards untreated cells, confirming asbestos as the mediator of oxidative stress evoked at the mesothelium level. These factors can therefore be considered predictive biomarkers of MPM and potential pharmacological targets in the treatment of this aggressive cancer.

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Mechanisms of Liver Injury. III. Oxidative stress in the pathogenesis of hepatitis C virus

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00522.2005 ◽

2006 ◽

Vol 290 (5) ◽

pp. G847-G851 ◽

Cited By ~ 204

Author(s):

Jinah Choi ◽

J.-H. James Ou

Keyword(s):

Oxidative Stress ◽

Hepatocellular Carcinoma ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Liver Injury ◽

Iron Overload ◽

Antioxidant Defense ◽

Hcv Infection ◽

Nitrogen Species ◽

Systemic Oxidative Stress

Hepatitis C virus (HCV) is a major cause of viral hepatitis that can progress to hepatic fibrosis, steatosis, hepatocellular carcinoma, and liver failure. HCV infection is characterized by a systemic oxidative stress that is most likely caused by a combination of chronic inflammation, iron overload, liver damage, and proteins encoded by HCV. The increased generation of reactive oxygen and nitrogen species, together with the decreased antioxidant defense, promotes the development and progression of hepatic and extrahepatic complications of HCV infection. This review discusses the possible mechanisms of HCV-induced oxidative stress and its role in HCV pathogenesis.

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The effect of one night of continuous positive airway pressure therapy on oxidative stress and antioxidant defense in hypertensive patients with severe obstructive sleep apnea

Sleep And Breathing ◽

10.1007/s11325-011-0531-z ◽

2011 ◽

Vol 16 (2) ◽

pp. 499-504 ◽

Cited By ~ 10

Author(s):

Mohammed A. Alzoghaibi ◽

Ahmed S. BaHammam

Keyword(s):

Oxidative Stress ◽

Obstructive Sleep Apnea ◽

Continuous Positive Airway Pressure ◽

Airway Pressure ◽

Antioxidant Defense ◽

Positive Airway Pressure ◽

Severe Obstructive Sleep Apnea ◽

Pressure Therapy ◽

Hypertensive Patients ◽

Obstructive Sleep

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Supplemental Selenium and Boron Mitigate Salt-Induced Oxidative Damages in Glycine max L.

Plants ◽

10.3390/plants10102224 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2224

Author(s):

Mira Rahman ◽

Khussboo Rahman ◽

Khadeja Sultana Sathi ◽

Md. Mahabub Alam ◽

Kamrun Nahar ◽

...

Keyword(s):

Oxidative Stress ◽

Salt Stress ◽

Antioxidant Defense ◽

Foliar Application ◽

Growth Parameters ◽

Monodehydroascorbate Reductase ◽

Ros Scavenging ◽

H2o2 Content ◽

Mda Content ◽

Different Levels

The present investigation was executed with an aim to evaluate the role of exogenous selenium (Se) and boron (B) in mitigating different levels of salt stress by enhancing the reactive oxygen species (ROS) scavenging, antioxidant defense and glyoxalase systems in soybean. Plants were treated with 0, 150, 300 and 450 mM NaCl at 20 days after sowing (DAS). Foliar application of Se (50 µM Na2SeO4) and B (1 mM H3BO3) was accomplished individually and in combined (Se+B) at three-day intervals, at 16, 20, 24 and 28 DAS under non-saline and saline conditions. Salt stress adversely affected the growth parameters. In salt-treated plants, proline content and oxidative stress indicators such as malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were increased with the increment of salt concentration but the relative water content decreased. Due to salt stress catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glyoxalase I (Gly I) and glyoxalase II (Gly II) activity decreased. However, the activity of ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST) and peroxidase (POD) increased under salt stress. On the contrary, supplementation of Se, B and Se+B enhanced the activities of APX, MDHAR, DHAR, GR, CAT, GPX, GST, POD, Gly I and Gly II which consequently diminished the H2O2 content and MDA content under salt stress, and also improved the growth parameters. The results reflected that exogenous Se, B and Se+B enhanced the enzymatic activity of the antioxidant defense system as well as the glyoxalase systems under different levels of salt stress, ultimately alleviated the salt-induced oxidative stress, among them Se+B was more effective than a single treatment.

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