scholarly journals Automated system of seedling image analysis (SVIS) and electrical conductivity to assess sun hemp seed vigor

2012 ◽  
Vol 34 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Clíssia Barboza da Silva ◽  
Magnólia de Mendonça Lopes ◽  
Julio Marcos-Filho ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seed Vigor ◽  
Automated System ◽  
Accurate Information ◽  
Hemp Seed ◽  
Control Programs ◽  
Seed Industry ◽  
Vigor Index

The development of new procedures for quickly obtaining accurate information on the physiological potential of seed lots is essential for developing quality control programs for the seed industry. In this study, the effectiveness of an automated system of seedling image analysis (Seed Vigor Imaging System - SVIS) in determining the physiological potential of sun hemp seeds and its relationship with electrical conductivity tests, were evaluated. SVIS evaluations were performed three and four days after sowing and data on the vigor index and the length and uniformity of seedling growth were collected. The electrical conductivity test was made on 50 seed replicates placed in containers with 75 mL of deionised water at 25 ºC and readings were taken after 1, 2, 4, 8 and 16 hours of imbibition. Electrical conductivity measurements at 4 or 8 hours and the use of the SVIS on 3-day old seedlings can effectively detect differences in vigor between different sun hemp seed lots.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Seedling image analysis and traditional tests to evaluate onion seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Nayara Roberto Gonçalves ◽  
Silvio Moure Cicero ◽  
Haynna Fernandes Abud
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Onion Seed ◽  
Emergence Percentage

Abstract: The aim of this study was to verify the possibility of using computerized seedling image analysis to detect differences in vigor among onion seed lots and to compare this analysis with traditional vigor tests. We used five seed lots of two onion cultivars, Bella Catarina and Bella Vista. The Seed Vigor Imaging System (SVIS®) software was used to process the seedling images and assess seedling length and vigor through seedling uniformity and growth indexes. These determinations were compared to traditional tests used to evaluate onion seed vigor: germination percentage and first germination count, seedling emergence percentage and emergence speed, accelerated aging, and electrical conductivity. Results showed that vigor differences among onion seed lots can be identified using the SVIS® in a manner similar to traditional vigor tests; assessments should be performed at five days after sowing.

scholarly journals Computerized imaging analysis of seedlings for assessment of physiological potential of wheat seeds

2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Relative Humidity ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Cultivars ◽  
Imaging Analysis ◽  
Wheat Seeds

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.

scholarly journals Digital images of seedling for evaluating coffee seed vigor

2019 ◽  
Vol 41 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Heiber Andres Trujillo ◽  
Francisco Guilhien Gomes-Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Imaging System ◽  
Primary Root ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Scanned Images ◽  
Coffea Arabica L ◽  
Coffee Seed

Abstract: The digital image analysis of seedlings has become largely employed in seed quality-control programs due to its feasibility, objectivity and fast results. Despite these advantages, no studies have yet demonstrated the efficiency of this technique for analyzing coffee seed vigor. The present study aimed at evaluating the efficiency of the Seed Vigor Imaging System (SVIS®) in determining the vigor of coffee seeds (Coffea arabica L.) from digital images of seedlings. Also, the results of these analyses were contrasted with conventional vigor tests. Six seed lots from each cultivar, Bourbon and Catucaí 20/15, were used. The research was conducted in two experimental times, and the seed vigor was determined by the tests of first germination count, accelerated aging, electrical conductivity, primary root protrusion speed, as well as by the analysis of scanned images of seedlings, made possible by the SVIS® software. For the Bourbon and Catucaí 20/15 cultivars, the SVIS® analysis was able to discriminate lots of both high and low vigor (vigor indexes of 317 to 752, and 181 to 703, respectively). A similar outcome was obtained from the conventional tests appraised in this research. Eventually, it was possible to conclude that the digital analysis of 20-day-old seedlings by SVIS® is an efficient method to evaluate coffee seed vigor.

scholarly journals Vigor evaluation of stored cotton seeds, including the Seed Vigor Imaging System (SVIS®)

2014 ◽  
Vol 36 (2) ◽  
pp. 222-230 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho
Keyword(s):  
Environmental Conditions ◽  
Cotton Seed ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Automated Analysis ◽  
Seed Vigor ◽  
Vigor Index ◽  
Before And After ◽  
Cotton Seeds

This study aimed at evaluating efficiency of different tests to assess cotton seed vigor during storage, including the SVIS® software. Thereby, five cotton seed lots (cv. BRS 293) were stored under controlled environmental conditions of low temperature and relative humidity (10 ºC; 30% RH), and under non-controlled laboratory environmental conditions, for eight months. Evaluations of germination, first germination count, cool germination, accelerated aging test traditional and with saturated salt, field seedling emergence and seedling imaging automated analysis - SVIS® were performed before and after every two storage months. It was concluded that the cool germination, accelerated aging (traditional and with saturated salt), field seedling emergence and seedlings length (SVIS®) are adequate parameters for assessing vigor of cotton seeds during storage, and that both accelerated aging and SVIS® (vigor index and seedlings length) present enough sensitivity to identify changes on seed vigor during storage.

scholarly journals Electrical Conductivity Test to Evaluate the Physiological Quality of Salvia hispanica L. Seeds

2018 ◽  
Vol 10 (3) ◽  
pp. 377
Author(s):  
A. K. Radke ◽  
D. W. Maass ◽  
M. S. Pieper ◽  
F. M. Xavier ◽  
A. B. N. Martins ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seed Vigor ◽  
Herbaceous Plant ◽  
Salvia Hispanica ◽  
Control Programs ◽  
Chia Seed ◽  
Physiological Quality ◽  
Salvia Hispanica L ◽  
Different Levels

Chia (Salvia hispanica L.) is an herbaceous plant that belongs to the Lamiaceae family. Seed vigor testing is an important component of quality control programs, and electrical conductivity test is a possible option in this process. The objective of this study was to identify a methodology of electrical conductivity test to stratify chia seed lots at vigor level according to the emergence of seedlings. Six lots of chia seeds were used in this study. The seeds were exposed to the following determinations: first germination count, germination, emergence speed index, emergence, aerial and root length, aerial and root dry matter, and electrical conductivity at 25, 50 and 75 mL during 1, 2, 4, 6, 8 and 24 hours of soaking in water. The electrical conductivity test in the combination of 25 seeds using 50 mL of water in 6-hour of soaking period, as well as the combination of 50 seeds using 50 mL of water for 24 hours of soaking, were efficient in the stratification of chia seeds in different levels of vigor.

scholarly journals Electrical Conductivity Testing as Applied to the Assessment of Freshly Collected Kielmeyera coriacea Mart. Seeds

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Kennya Mara Oliveira Ramos ◽  
Juliana M. M. Matos ◽  
Rosana C. C. Martins ◽  
Ildeu S. Martins
Keyword(s):  
Electrical Conductivity ◽  
Quality Control ◽  
Constant Temperature ◽  
Seed Quality ◽  
Seed Vigor ◽  
Promising Method ◽  
Control Programs ◽  
Kielmeyera Coriacea

Assessment of seed vigor has long been an important tool of seed quality control programs. The conductivity test is a promising method for assessment of seed vigor, but proper protocols for its execution have yet to be established. The objective of this study was to assess the efficiency of electrical conductivity (EC) testing as a means of assessing the viability of freshly collected Kielmeyera Coriacea Mart. seeds. The test was performed on individual seeds rather than in a bulk configuration. Seeds were soaked for different periods (30 min, 90 min, 120 min., 180 min, and 240 min) at a constant temperature of 25°C. Conductivity was then measured with a benchtop EC meter.

scholarly journals Controlled deterioration test and use of the Seed Vigor Imaging System (SVIS ® ) to evaluate the physiological potential of crambe seeds

2017 ◽  
Vol 39 (4) ◽  
pp. 393-400 ◽  
Author(s):  
Erica Fernandes Leão-Araújo ◽  
Juliana Faria dos Santos ◽  
Clíssia Barboza da Silva ◽  
Júlio Marcos-Filho ◽  
Roberval Daiton Vieira
Keyword(s):  
Moisture Content ◽  
Imaging System ◽  
Seedling Development ◽  
Seed Vigor ◽  
Test Results ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Vigor Index ◽  
Controlled Deterioration ◽  
Consistency Of The Test

Abstract: Vigor tests are important tools for verifying the physiological potential of seed lots; however, various aspects can interfere in the consistency of the test results. The search for less subjective procedures has drawn the interest of researchers. The aim of this study was to adapt the methodology of the controlled deterioration test and verify the efficiency of the Seed Vigor Imaging System (SVIS ® ) to evaluate the vigor of crambe seeds. Initially, seed moisture content was determined and germination and vigor were evaluated in five seed lots. For the controlled deterioration test, the seed moisture content was adjusted to 18%, 20%, and 22%, and the results were interpreted at four and five days after sowing. The controlled deterioration test should be performed with initial adjustment of seed moisture content to 18%, and the germination test should be evaluated on the fifth day after sowing. SVIS ® provided information on the vigor index and the length and uniformity of seedling development. Computerized image analysis is effective in evaluating the vigor of crambe seeds, and the uniformity of seedling development parameter offers the most consistent data.

scholarly journals Physiological potential of cauliflower seeds

2008 ◽  
Vol 65 (4) ◽  
pp. 374-380 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Laboratory Tests ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Controlled Deterioration ◽  
Favorable Conditions ◽  
Consistent Information ◽  
Potassium Leakage

The assessment of seed physiological performance is essential to provide consistent information to compose quality control programs conducted by seed companies and allow prompt decisions to reduce post-harvest and commercialization problems. This research was carried to evaluate the efficiency of different procedures to determine cauliflower seed vigor. Six lots of the cultivar Sharon were initially characterized by germination and seedling emergence, and subsequently subjected to traditional and saturated salt accelerated aging, controlled deterioration, electrical conductivity, and potassium leachate tests, in two evaluation times. The traditional accelerated aging (41ºC/48 h), the saturated salt procedure (45ºC/72 h), the controlled deterioration (moisture content adjusted to 20% or 22% and aging at 45ºC/24 h), the electrical conductivity (50 seeds/75 mL water and evaluation after 4 h) and the potassium leakage (25 seeds/50 mL of water and evaluation after 30 min) laboratory tests are sensitive to detect differences in the physiological potential of cauliflower seeds. However, those results did not relate to the percentage seedling emergence in greenhouse controlled conditions, as a consequence of the favorable conditions for emergence. However, the differences identified in the tests may be related to the ability of seed lots to perform in less favorable environments.

scholarly journals Computerized image analysis of seedlings to evaluate broccoli seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Haynna Fernandes Abud ◽  
Silvio Moure Cicero ◽  
Francisco Guilhien Gomes Junior
Keyword(s):  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Computer Image Analysis ◽  
Computerized Image Analysis ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract: The computerized image analysis of seedlings constitutes an efficient technique to evaluate the quality of seed lots, since it is quick in responding, has a simple execution and reproduction. The goal of this research was to verify the efficiency of the computer image analysis of seedlings to detect vigor differences among broccoli seed lots and compare them with other traditionally used vigor tests. Computerized image analysis were performed by the Seed Vigor Imaging System (SVIS®), using 3-day-old seedlings and the results were compared to germination at first count, seedling emergence, accelerated aging and electrical conductivity tests. Evaluations were performed in three experimental periods (before storage, 6 and 12 months of storage at 20 °C and 45-50% RH). A completely randomized design with eight replications of 25 seeds was used. Data were submitted to analysis of variance and means were compared by Scott-Knott test at 5% error probability. It is concluded that it is possible to detect vigor differences among broccoli seed lots by the computerized image analysis of seedlings with SVIS®, similarly to traditional vigor tests.

Sign in / Sign up

Export Citation Format

Share Document