scholarly journals Biochemical descriptors: importance of the genetic divergence study in peppers

2019 ◽  
Vol 37 (2) ◽  
pp. 210-214
Author(s):  
Lourismar M Araújo ◽  
Leonarda G Neves ◽  
Demétrio A Sousa ◽  
Walmes M Zeviani ◽  
Lucinéia da R Silva ◽  
...  

ABSTRACT The genetic diversity of pepper genotypes is usually evaluated based on morphological and molecular descriptors. However, studies using biochemical descriptors of peppers are still incipient. Thus, the objective of this study was identify biochemical descriptors of Capsicum spp. with antioxidant action, most responsive to the selection of superior genotypes, and evaluate the correlation between concentrations of the parameters using principal components analysis. The experiment was conducted in the city of Cáceres, southwest Mato Grosso, Brazil. Seventy-eight pepper accessions from the Active Germplasm Bank of UNEMAT were planted in randomized block design. The harvested fruits were immersed in liquid nitrogen for two minutes, and the following biochemical analyses were performed: titratable acidity, ascorbic acid, total phenols, flavonoids, anthocyanins, carotenoids, chlorophyll a, chlorophyll b, and antioxidant activity. Biochemical descriptors that best estimated the genetic variability of the accessions were flavonoids, total phenols, chlorophyll a, and ascorbic acid. Chlorophyll a and chlorophyll b were the compounds that showed the highest degree of correlation. Except chlorophyll a with ascorbic acid, all other vectors showed correlation ranging from moderate to strong.

2021 ◽  
Vol 29 ◽  
pp. 204-210
Author(s):  
Gustavo Soares Wenneck ◽  
Reni Saath ◽  
Roberto Rezende ◽  
Lucas Henrique Maldonado da Silva

The measurement of leaf pigments using non-destructive methods can be influenced by water management conditions and the use of nutrients in cauliflower, not showing adequate correlations with chlorophyll contents. The objective of this study was to evaluate the correlation between the SPAD index and the pigment content in cauliflower leaves managed with different water conditions and the application of silicon (Si). The cultivation was carried out in a protected environment in the city of Maringá, Paraná State from October 2019 to March 2020. It was adopted a randomized block design, with treatments in a 3 x 4 factorial scheme, with three levels of water recharge (40; 70 and 100% of crop evapotranspiration (ETc)) and four doses of Si (0; 50; 100 and 150 kg ha-1), with four replications. Once plants showed developed inflorescence, the SPAD index was determined in the field and the levels of chlorophyll a, chlorophyll b, and carotenoids in the laboratory using leaves from the upper third. The correlation between water recharge and Si fertilization on the SPAD index was determined. The proportion of carotenoids in relation to total pigments was compared under different conditions. Correlation analysis was performed considering silicon fertilization, water recharge, leaf pigments, and the SPAD index. Silicon fertilization reduced the levels of carotenoids in addition to being related to chlorophyll a (100% of ETc) and chlorophyll b (70% of ETc). The correlation of the SPAD index with pigments is variable with the water condition of the crop, in a condition without water deficit, it is related to chlorophyll a and in a condition of 70% replacement of ETc, it is correlated with chlorophyll b.


2019 ◽  
Vol 37 (2) ◽  
pp. 138-145
Author(s):  
Marcelo de A Guimarães ◽  
Manuel Filipe N Garcia ◽  
Jean Paulo de J Tello ◽  
Hozano de S Lemos Neto ◽  
Benedito P Lima Neto ◽  
...  

ABSTRACT Grafting has been considered an important tool to produce tomato in adverse environments. Therefore, the aim of this study was to develop grafting protocols for tomato on rootstocks of Jilo, Cocona and Jurubeba. The work was carried out in a randomized block design, in a factorial scheme (3x2) + 1 with three grafting methods (slant-cut grafting, cleft grafting and splice grafting), combined with two graft ages and rootstocks (depending on the species), and the control. The rootstocks for tomato (Solanum lycopersicum) cv. Santa Clara were: 1) Jilo (S. gilo) cv. Comprido Grande Rio; 2) Cocona (S. sessiliflorum) and 3) Jurubeba (S. jamaicense). The methods were individually studied and the graft length (GL); chlorophyll ‘a’ and chlorophyll ‘b’ in pre and post-grafting; graft diameter (GD), pre-grafting and tissue union of the grafted individuals through the assignment of grades were evaluated. Seven days after grafting, evaluations of grafted plants were performed. It was possible to establish a grafting protocol for all the combinations. The rootstock of Cocona provided greater vigor to ‘Santa Clara’ tomato graft. The cleft grafting technique can be indicated as the most effective for tomato grafting on the studied Solanaceae species.


2020 ◽  
Vol 8 (2) ◽  
pp. 234
Author(s):  
Made Hary Sayoga ◽  
Ni Made Wartini ◽  
Lutfi Suhendra

 Fragrant pandan leaves can be used as a natural coloring because they contain chlorophyll obtained through the extraction process. The purposes of this study were to determine the effect of particle size and extraction time on the characteristics of natural pandanus fragrance leaf extract and determine the best treatment of particle size and extraction time to produce natural pandanus leaf extract dye. This study used a 2 factorial factorial randomized block design. The first factor is the particle size which consists of 3 levels, namely 40, 60, 80 mesh. The second factor is the extraction time which consists of 3 levels, namely 24, 36, and 48 hours. The results showed that the treatment of particle size and extraction time affected the yield, chlorophyll a, chlorophyll b, total chlorophyll, brightness (L *), redness (a *) and yellowish level (b *). The treatment of 60 mesh particle size and 36 hour extraction time are the best treatment to produce natural pandanus fragrance extract coloring with yield characteristics of 8.81% chlorophyll a level of 23.66%, chlorophyll b level of 3.92%, total chlorophyll content of 27.57%, the brightness level (L *) 21.06, the redness level (a *) 4.92, the yellowish level (b *) 6.21. Keywords: Pandanus amaryllifolius R., chlorophyll, color, extraction


2021 ◽  
Vol 9 (1) ◽  
pp. 53
Author(s):  
Rian Lamhot Parasian Hutabarat ◽  
Ni Made Wartini ◽  
Nyoman Semadi Antara

This study aims to determine the effect of type of solvent and maceration temperature on the characteristics of cassava leaves extracts as a natural dyes leaves and determine the type of solvent for cassava leaf powder and the best maceration temperature to obtain the extract of natural cassava leaves. This study used a factorial randomized block design with 2 factors, namely the type of solvent (85% ethanol, 85% acetone and mixture (85% ethanol: 85% acetone) and maceration temperature (35 ° C, 45 ° C and 55 ° C). The data obtained were analyzed with variance and if the treatment had a significant effect, then continued with the Tukey test. The results showed that the treatment interactions had a very significant effect on yield, chlorophyll b, brightness (L *), yellowish level (b *), total chlorophyll, chlorophyll a. , redness (a *), but had no significant effect on the interaction at the level of redness (a *). The treatment of 85% acetone solvent and 55 ° C maceration temperature was the best treatment to produce acetone extract of natural dye of cassava leaves with yield characteristics of 14, 04%, total chlorophyll 12.10%, chlorophyll a 4.39%, chlorophyll b 7.71%, brightness (L *) 16.46, redness (a *) 9.77 yellowish (b *) of 5.70.brightness level (L*) of 11.03, redness level (a*) of 10.29, yellowish level (b*) of 2.83. Key words: cassava leaves, dye extract, maceration temperature, natural dye, solvent type.


2020 ◽  
Vol 5 (2) ◽  
pp. 49
Author(s):  
Ezra Elkana Karo Sekali ◽  
Ni Made Wartini ◽  
Lutfi Suhendra

Penelitian ini bertujuan untuk  mengetahui pengaruh ukuran partikel dan lama maserasi terhadap karakteristik ekstrak aseton pewarna alami daun singkong dan menentukan ukuran partikel dan lama maserasi terbaik untuk mendapatkan ekstrak aseton pewarna alami daun singkong. Penelitian ini menggunakan Rancangan Acak Kelompok Pola Faktorial dengan 2 Faktor yaitu Ukuran Partikel (40 mesh, 60 mesh, 80 mesh) dan Lama Maserasi (24 jam, 36 jam, 48 jam). Data yang diperoleh dianalisis dengan sidik ragam dan apabila perlakuan berpengaruh nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa interaksi perlakuan berpengaruh sangat nyata terhadap rendemen, klorofil b, tingkat kecerahan (L*), tingkat kekuningan (b*) tetapi berpengaruh tidak nyata terhadap klorofil total, klorofil a, kemerahan (a*). Perlakuan ukuran partikel 80 mesh dan lama maserasi 36 jam merupakan perlakuan terbaik untuk menghasilkan ekstrak aseton pewarna alami daun singkong dengan karakteristik rendemen sebesar 8,08%, klorofil total sebesar 3,83%, klorofil a sebesar 2,05%, klorofil b sebesar 1,77%, tingkat kecerahan (L*) sebesar 11,03, tingkat kemerahan (a*) sebesar 10,29, tingkat kekuningan (b*) sebesar 2,83.   This study aims to know the effect of particle size and maceration time on the characteristics of the natural dye acetone extract of cassava leaves and determine the best particle size and maceration time to obtain the acetone extract of natural coloring cassava leaves.This research used a randomized block design with factorial pattern with two factors namely, particle size (40 mesh, 60 mesh, 80 mesh) and maceration time (24 hours, 36 hours, 48 hours). The data obtained were analyzed by analysis of variance. If the treatment had a significant effect, it would be followed by the Duncan test. The result showed that interactions had significant effect on theextract yield, chlorophyll b levels, brightness level L*, yellowish level b*, but had no effect on total chlorophyll levels, chlorophyll a levels, redness level a*. Treatment of particle size 80 mesh and maceration time 36 hours, is the best treatment to produce aceton extract natural dyes of cassava leaves with extract yield characteristics of 8.08%,total chlorophyll levels of 3.83, chlorophyll a levels of 2.05%,chlorophyll b levels of 1.77%,brightness level (L*) of 11.03,redness level (a*) of 10.29,yellowish level (b*) of 2.83.


Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 448
Author(s):  
Leontina Lipan ◽  
Aarón A. Carbonell-Pedro ◽  
Belén Cárceles Rodríguez ◽  
Víctor Hugo Durán-Zuazo ◽  
Dionisio Franco Tarifa ◽  
...  

Mango is one of the most cultivated tropical fruits worldwide and one of few drought-tolerant plants. Thus, in this study the effect of a sustained deficit irrigation (SDI) strategy on mango yield and quality was assessed with the aim of reducing irrigation water in mango crop. A randomized block design with four treatments was developed: (i) full irrigation (FI), assuring the crop’s water needs, and three levels of SDI receiving 75%, 50%, and 33% of irrigation water (SDI75, SDI50, and SDI33). Yield, morphology, color, titratable acidity (TA), total soluble solids (TSS), organic acids (OA), sugars, minerals, fiber, antioxidant activity (AA), and total phenolic content (TPC) were analyzed. The yield was reduced in SDI conditions (8%, 11%, and 20% for SDI75, SDI50, and SDI33, respectively), but the irrigation water productivity was higher in all SDI regimes. SDI significantly reduced the mango size, with SDI33 generating the smallest mangoes. Peel color significantly changed after 13 days of ripening, with SDI75 being the least ripe. The TA, AA, and citric acid were higher in SDI75, while the TPC and fiber increased in all SDI levels. Consequently, SDI reduced the mango size but increased the functionality of samples, without a severe detrimental effect on the yield.


2013 ◽  
Vol 31 (1) ◽  
pp. 08-14 ◽  
Author(s):  
Edilson Costa ◽  
Taynara G de Souza ◽  
Gleciane de L Benteo ◽  
Katiane SS Benett ◽  
Cleiton GS Benett

The okra grows well in hot weather, finding favorable conditions for development in Brazil. We evaluated the production of okra seedlings and productivity. The experiment was conducted in the experimental area of the State University of Mato Grosso do Sul, in Aquidauana. In the seedling phase we tested substrates (100% triturated cassava stems, 75% triturated cassava stems and 25% vermiculite, 50% triturated cassava stems and 50% vermiculite, 25% triturated cassava stems and 75% vermiculite and 100% vermiculite). These substrates were placed in two protected environments (nursery with black screen, 50% of shading, and aluminized screen, 50% of shading). Each environment was considered an experiment conducted in completely randomized design with eight replications. After that we realized joint analysis. In the field, a total of 10 treatments generated from combinations of environments and substrates (2 environments x 5 substrates) were distributed in a randomized block design with four replications. The average proportions of cassava stems and vermiculite may be suitable for okra seedlings. The results of shoot and total dry phytomass indicate that the aluminized screen can be recommended for okra seedlings production when the substrate with a 1:1 ratio is used. Seedlings grown on higher percentages of vermiculite anticipate production, while seedlings produced only in cassava stems tend to slow it down. The aluminized screen tends to increase the yield of okra seedlings. Average proportions of vermiculite and cassava stems for seedling production under aluminized screen, provide more fruits and yield of okra in Aquidauana.


Irriga ◽  
2018 ◽  
Vol 21 (3) ◽  
pp. 561
Author(s):  
Lígia Borges Marinho ◽  
José Antonio Frizzone ◽  
João Batista Tolentino Junior ◽  
Janaína Paulino ◽  
José Monteiro Soares ◽  
...  

DÉFICIT HÍDRICO NAS FASES VEGETATIVA E DE FLORAÇÃO DA PIMENTA ‘TABASCO’ EM AMBIENTE PROTEGIDOLÍGIA BORGES MARINHO 1; JOSÉ ANTONIO FRIZZONE2; JOÃO BATISTA TOLENTINO JUNIOR3; JANAÍNA PAULINO4; JOSÉ MONTEIRO SOARES5 E FRANCISCO NOGUEIRA VILAÇA6*Artigo extraído da tese do primeiro autor1 Enga. Agrônoma, Doutora, Profa. Departamento Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, av. Edgard Chastinet, São Geraldo, CEP 48905-680, Juazeiro, BA. Fone (74) 3611-7363. E-mail: [email protected] Eng. Agrônomo, Doutor, Prof. Departamento de Engenharia de Biossistemas/Escola Superior de Agricultura “Luiz de Queiroz” USP, Av. Pádua Dias, 11, CEP 13.418-900, Piracicaba/SP, E-mail(s): [email protected];3 Eng. Agrônomo, Doutor, Prof. Campus Curitibanos, Universidade Federal de Santa Catarina, Curitibanos, SC. E-mail: [email protected] Enga. Agrícola, Doutora, Profa. Universidade Federal de Mato Grosso UFMT, campus Sinop, Avenida Alexandre Ferronato Nº 1.200. Bairro: Setor Industrial. CEP: 78.550-000, Sinop-MT, Email: [email protected] Eng. Agrônomo, Doutor, Pesquisador EMBRAPA/CPATSA, BR 428, Km 152, Zona Rural, Caixa Postal 23, CEP: 56310-000, Petrolina, PE, E-mail: [email protected] Eng. Agrônomo, Mestre. Departamento de Engenharia de Biossistemas/Escola Superior de Agricultura “Luiz de Queiroz” USP, Av. Pádua Dias, 11, CEP 13.418-900, Piracicaba/SP, E-mail: [email protected] RESUMOA escassez de recursos hídricos no Brasil tem impulsionado a demanda de manejo de irrigação mais eficiente nas propriedades agrícolas, visando reduzir o consumo de água e tornar o seu uso mais eficiente. O objetivo foi avaliar o efeito do déficit hídrico em diferentes fases do cultivo da pimenteira na eficiência de uso de água, na produtividade e na qualidade da pimenta ‘Tabasco’ cultivada em ambiente protegido, durante os meses de setembro de 2009 a julho de 2010, no município de Piracicaba-SP. O delineamento foi em blocos casualizados, com lâminas de irrigação de 40, 60, 80 e 100% da evapotranspiração da cultura (ETc), inicializadas nas fases vegetativa e de floração. O déficit de irrigação influenciou a produtividade da pimenta e o mesmo não foi observado para a qualidade física da pimenta. A eficiência do uso da água da pimenta Tabasco não foi influenciada pelo déficit. Entretanto, o déficit hídrico inicializado na fase de floração permitiu economia de água de até 50,1% de água no ciclo de 208 dias após transplantio.Palavras-chave: Estresse hídrico, Capsicum frutescens, produtividade e qualidade do fruto.MARINHO, L. B.; FRIZZONE, J. A.; TOLENTINO JÚNIOR, J. B.; PAULINO, J.; SOARES, J. M.; VILAÇA, F. N.WATER DEFICIT APPLIED IN VEGETATIVE AND FLOWERING STAGE OF ‘TABASCO’ PEPPER IN GREENHOUSE2 ABSTRACTThe scarcity of water resources in Brazil has driven the management of demand for more efficient irrigation in farms in order to reduce water consumption and make its use more efficient. The objective of this study was to evaluate the water deficits’ effect at the vegetative and flowering stages of pepper in water use efficiency, yield and quality of pepper cv. 'Tabasco'(Capsicum frutescens) grown in a greenhouse from September 2009 to July 2010. The experiment was conducted as randomized block design with irrigation levels of 40, 60, 80 and 100% of Crop Evapotranspiration (ETc), beginning at vegetative and flowering stages. There was a significant effect of full and deficits irrigation started at vegetative and flowering stages on the fruit number per plant, and the linear model was the best fitted. The average of mass fruit per plant did not vary with the deficit, not even with the start up times. The deficit irrigation affected pepper productivity, yet it was not observed on the physical quality of fruits. The deficit irrigation (40% ETc) beginning at flowering stage allowed savings of up to 49.8% in water consumption in a cycle of 208 days after transplanting.Keywords: water stress, Capsicum frutescens, productivity and fruit quality.


2020 ◽  
Vol 33 (02) ◽  
pp. 694-706
Author(s):  
Hassan Sarhadi ◽  
Jahanfar Daneshian ◽  
Seyyed Alireza Valadabadi ◽  
Hossein Heidary Sharafabad ◽  
Hossein Heidary Sharafabad ◽  
...  

The response of active ingredient, chlorophyll a and b contents, and chlorophyll index of henna to different rates of N fertilization and drought stress was explored in a split-split-plot experiment based on a randomized complete block design with four replications in the research farm of Jiroft Branch, Islamic Azad University, Jiroft, Iran in the 2016-2017 growing season. In the current study, the main plot was assigned to drought stress at three levels (including irrigation to supply 100%, 75% or 50% of plant water requirement), the sub-plot was assigned to N fertilization at three rates (50, 100 or 150 kg ha-1 pure N), and the sub-sub-plot was assigned to ecotype at three levels (Bami, Bushehri, and Rudbari). The results showed that the effect of drought level was significant on active ingredient and chlorophyll index at the p < 0.01 level and on chlorophyll a, total chlorophyll, and chlorophyll a/b ratio at the p < 0.05 level.  But, it could not influence chlorophyll b significantly. The effect of N fertilization was significant on most studied traits at the p< 0.01 level, but its effect was significant on the chlorophyll index at the p < 0.05 level and insignificant on dye and chlorophyll b. According to the results, it seems that the application of 100 kg ha-1 N and the irrigation to supply 75% of plant water requirement can contribute to having more fresh plants with the higher active ingredient and dye contents.


2018 ◽  
Vol 36 (3) ◽  
pp. 257-265
Author(s):  
Ana Gabriela de Sousa Basílio ◽  
Leonardo Vieira De Sousa ◽  
Toshik Iarley Da Silva ◽  
Joana Gomes De Moura ◽  
Anderson Carlos de Melo Gonçalves ◽  
...  

The use of saline or low-quality water in agriculture is an alternative to increasing water demand, especially in arid or semi-arid regions. However, the use of water with high levels of salts causes disturbances in plants, which can lead to their death; thus, alternatives to mitigate these effects are relevant in current agriculture. Currently, antioxidants are used to mitigate the effects of salts in plants, and among them ascorbic acid has been frequently mentioned. Therefore, the aim of this study was to evaluate the effect of irrigation with saline water combined with applications of ascorbic acid on the development and photosynthetic activity of radish (Raphanus sativus L.) plants. This experiment was carried out in a greenhouse with a randomized block design, with the treatments distributed in a 5.5 incomplete factorial scheme, composed of five electrical conductivities of the irrigation water (ECw): 0.50, 1.30, 3.25, 5.20 and 6.00 dS m-1, and five ascorbic acid (AA) doses: 0.00, 0.29, 1.00, 1.71, and 2.00 mM. The evaluated variables were: shoot height, leaf number, tuberous root diameter, chlorophyll a, b and total content, chlorophyll a/b ratio, initial fluorescence, maximum fluorescence, variable fluorescence and quantum yield of photosystem II. The saline water influenced the analyzed variables in the radish crop regardless of the ascorbic acid application. The ascorbic acid was not efficient in attenuating the deleterious effect of salinity in the irrigation water on the development and fluorescence of the radish. However, it was observed that the concentration of 1.00 mM of ascorbic acid promoted an increase in chlorophyll a, b and total in the saltstressed radish plants.


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