scholarly journals Narrow band spectral indexes for chlorophyll determination in soybean canopies [Glycine max (L.) Merril]

2004 ◽  
Vol 16 (3) ◽  
pp. 131-136 ◽  
Author(s):  
Clotilde Pinheiro Ferri ◽  
Antonio Roberto Formaggio ◽  
Marlene Aparecida Schiavinato

Photosynthetic pigments are essential for plant development. Quantifying these pigments in great extensions of agricultural crops is an important objective in remote sensing for agricultural purposes. This information can be used to produce a more accurate estimation of the physiological state of the vegetation, for species discrimination and productivity estimation. The aim of the present study was to (a) evaluate the potential for estimating chlorophyll content of crop canopies, using narrow band spectral indexes, and (b) in this respect compare the performances of NDVI (a multispectral wide band index) and two narrow band vegetation indexes (R750/700 and R750/550). Experiments were carried out under greenhouse conditions whereby soybean [Glycine max (L.), Merril] was monitored with a high-resolution spectroradiometer (10 nm at 365-1,126 nm range) during the phenological cycle of the crop. Chlorophyll (a, b and total) contents were determined weekly in the laboratory. A statistical correlation analysis was performed between narrow band spectral indexes against chlorophyll content and r² coefficients near 0.84 were obtained. For NDVI r² was around 0.51. These analyses showed that R750/700 and R750/550 ratios are very useful indexes for chlorophyll determination and very effective compared with NDVI (one of the wide band indexes widely used). Thus, it can be stated that hyperspectral remote sensing has great potential for providing a reliable estimate of photosynthetic pigment content at the canopy level through evaluated indexes and other such indexes that might arise. Thus, further studies should be carried out for evaluating other indexes at the canopy level, both in the laboratory and under field conditions, using spectroradiometers and hyperspectral images, aimed at providing information for agricultural purposes.

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1909
Author(s):  
John T. Sanders ◽  
Eric A. L. Jones ◽  
Robert Austin ◽  
Gary T. Roberson ◽  
Robert J. Richardson ◽  
...  

Field studies were conducted in 2016 and 2017 to determine if multispectral imagery collected from an unmanned aerial vehicle (UAV) equipped with a five-band sensor could successfully identify Palmer amaranth (Amaranthus palmeri) infestations of various densities growing among soybeans (Glycine max [L.] Merr.). The multispectral sensor captures imagery from five wavebands: 475 (blue), 560 (green), 668 (red), 840 (near infrared [NIR]), and 717 nm (red-edge). Image analysis was performed to examine the spectral properties of discrete Palmer amaranth and soybean plants at various weed densities using these wavebands. Additionally, imagery was subjected to supervised classification to evaluate the usefulness of classification as a tool to differentiate the two species in a field setting. Date was a significant factor influencing the spectral reflectance values of the Palmer amaranth densities. The effects of altitude on reflectance were less clear and were dependent on band and density being evaluated. The near infrared (NIR) waveband offered the best resolution in separating Palmer amaranth densities. Spectral separability in the other wavebands was less defined, although low weed densities were consistently able to be discriminated from high densities. Palmer amaranth and soybean were found to be spectrally distinct regardless of imaging date, weed density, or waveband. Soybean exhibited overall lower reflectance intensity than Palmer amaranth across all wavebands. The reflectance of both species within blue, green, red, and red-edge wavebands declined as the season progressed, while reflectance in NIR increased. Near infrared and red-edge wavebands were shown to be the most useful for species discrimination and maintained their utility at most weed densities. Palmer amaranth weed densities were found to be spectrally distinct from one another in all wavebands, with greatest distinction when using the red, NIR and red-edge wavebands. Supervised classification in a two-class system was consistently able to discriminate between Palmer amaranth and soybean with at least 80% overall accuracy. The incorporation of a weed density component into these classifications introduced an error of 65% or greater into these classifications. Reducing the number of classes in a supervised classification system could improve the accuracy of discriminating between Palmer amaranth and soybean.


2010 ◽  
Vol 36 (2) ◽  
pp. 242-248
Author(s):  
Guang-Jun LI ◽  
He-Nan LI ◽  
Li-Guo CHENG ◽  
Yuan-Ming ZHANG

2019 ◽  
Vol 24 (2) ◽  
pp. 97-107
Author(s):  
Yunia Vella Alfani ◽  
Pudjawati Suryatmana ◽  
Ade Setiawan

This study was aimed at determining the increasing of growth and production of soybean crops by giving Azotobacter sp. and additive materials such as coconut water, molasses, and bran. This research was conducted on Januari 2018 to April 2018 at Ciparanje Experimental Field Faculty of Agriculture. Universitas Padjadjaran, Jatinangor, Sumedang, West Java Province, on ± 774 meters above sea level (asl) using single factor randomized block design with 10 treatments; Azotobacter sp. and additive materials combination such as coconut water, molasses, and bran in three repetitions. The observations on the parameters observed in the final vegetative phase were on plant height, chlorophyll content, and population of Azotobacter sp. using Total Plate Count (TPC) method.The results show that the application of combination additives materials and Azotobacter sp. effect on populations Azotobacter sp., chlorophyll content, plant height and number of soybean pods (Glycine max L.). Application of coconut water independently, water added with Azotobacter sp., and combination of additive with Azotobacter sp. can give the best effect to the content of chlorophyll, plant height, and soybean crops components (Glycine max. L.).PENGARUH KOMBINASI BAHAN ADITIF DAN Azotobacter sp. TERHADAP PERTUMBUHAN Glycine max. L.Penelitian ini bertujuan untuk mengetahui pertumbuhan dan produksi tanaman kedelai dengan memberikan Azotobacter sp. dan aditif air kelapa, molase, serta dedak. Penelitian ini dilaksanakan pada Januari 2018-April 2018 di Kebun Percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jawa Barat pada ± 774 meter di atas permukaan laut (dpl). Penelitian ini menggunakan rancangan acak kelompok dengan 10 perlakuan pemberian Azotobacter sp. dan aditif air kelapa, molase, serta dedak dengan tiga ulangan. Pengamatan terhadap parameter yang diamati pada fase vegetatif akhir yaitu tinggi tanaman, kandungan klorofil, dan populasi Azotobacter sp. dengan metode Total Plate Count (TPC). Hasil penelitian menunjukkan aplikasi kombinasi bahan aditif dan penambahan pupuk hayati Azotobacter sp. berpengaruh terhadap populasi Azotobacter sp., kadar klorofil, tinggi tanaman dan jumlah polong tanaman kedelai (Glycine max L.). Aplikasi air kelapa secara mandiri, air kelapa yang ditambahkan dengan Azotobacter sp., dan kombinasi bahan aditif dengan Azotobacter sp. dapat memberikan pengaruh terbaik terhadap kandungan klorofil, tinggi tanaman, dan komponen hasil tanaman kedelai (Glycine max. L.). 


2021 ◽  
Vol 24 (2) ◽  
pp. 33-46
Author(s):  
MS Rahman ◽  
MA Malek ◽  
RM Emon ◽  
A Hannan ◽  
GHM Sagor

Three advanced lines (SB02, SB05, SB07) along with one tolerant (Lokon) and one susceptible check (Asswt) of soybean (Glycine max L.) were assessed for salt tolerance in terms of morpho-physiological traits and molecular markers (SSR). The experiment was conducted at seedling stage with four salinity treatments namely 0, 8, 12 and 16 dSm-1 following Completely Randomized design. All the genotypes displayed considerable reduction in their morphological traits, least affecting the tolerant one. None of the genotypes were survived at 12 and 16 dSm-1 stress condition. Among the lines tested, SB-02 and SB-05 were identified as salt tolerant at 8 dSm-1 based on salinity susceptibility index (SSI) scoring. These genotypes suffered less in reduction of leaf chlorophyll content (SPAD) and increase of Na+/K+ than the susceptible genotypes. For all the traits viz. shoot length, root length, total length, shoot fresh weiht, root fresh weight, shoot dry weight, root dry weight, total fresh weight, total dry weight, percent live leaves, chlorophyll content and Na+/K+ ratio, the phenotypic coefficient of variation (PCV) was higher than that of genotypic coefficient of variation (GCV). All the traits studied showed medium to high heritability ranging between 43.81% (SPAD) to 96.65% (shoot length). The genotypes were grouped into two clusters considering both Euclidian distance and Unweighted Pair Group Method with Arithmetic Mean analysis. Lokon, SB-02 and SB-05 are on the same cluster as tolerant, and SB-7 and Asset on the other as susceptible to salt stress. The molecular pattern using by SSR marker displayed an average number of 3.33 alleles per locus with PIC (Polymorphism Information Content) values ranged from 0.2688 (sat_655 and satt728) to 0.7680 (sat_210). The highest gene diversity was observed in sat_210 and satt237 and the lowest in sat_655 and satt728 with a mean diversity of 0.5733. The genotypes Lokon, SB-02 and SB-05 could be suggested as a potential germplasm source of QTL (Quantitative Trait Loci) analysis for the development of salt tolerant soybean variety. Ann. Bangladesh Agric. (2020) 24(2): 33-46


2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Bo Kong ◽  
Bing He ◽  
Huan Yu ◽  
Yu Liu

Stipa purpurea is the representative type of alpine grassland in Tibet and the surviving and development material for herdsmen. This paper takes Shenzha County as the research area. Based on the analysis of typical hyperspectral variables sensitive to chlorophyll content of Stipa purpurea, 10 spectral variables with significant correlation with chlorophyll were extracted. The estimation model of chlorophyll was established. The photosynthetic pigment contents in the Shenzha area were calculated by using HJ-1A remote sensing images. The results show that (1) there are significant correlations between chlorophyll content and spectral variables; in particular, the coefficient of Chlb in Stipa purpurea with RVI is the largest (0.728); (2) 10 variables are correlated with chlorophyll, and the order of correlation is Chlb > Chla > Chls; (3) for the estimation of Chla, the EVI is the best variable. RVI, NDVI, and VI2 are suitable for Chlb; RVI and NDVI are also suitable for the estimation of Chls; (4) the mean estimated content of Chla in Stipa bungeana is about 4.88 times that of Chlb, while Cars is slightly more than Chlb; (5) the distribution of Chla is opposite to Chlb and Chls content in water area.


The effect of different periods of red-light (RL, 660 nm) irradiation of plants on the biomass, leaf area, chlorophyll content, level of the Hill reaction and photophosphorylation in short-day (SD) and photoperiodical neutral (PhPN) lines of soybean have been studied in vegetation experiments. The objects of study were the isogenic lines (E genes) of soybean (Glycine max (L.) Merr.), Clark variety. The SD (E1E2E3) and PhPN lines (e1e2e3) were used. The plants were grown in a vegetation chamber in the soil culture (black soil). Plants of SD and PhPN lines were grown in 9 vessels of three liters volume. 10–12 plants were grown in each vessel. The constant growing conditions were provided during the experiment: temperature – 20–24/17–20°С (day/night), soil moisture – 60–70 % of the total soil moisture content, intensity of illumination – 20 klx, photoperiod duration – 10 hours. After 4–5 weeks of vegetation (after the second true leaf formation), plants of each line in three vessels were irradiated for 30 minutes with low intensity red light at the beginning (experiment 1) or in the middle of the dark period (experiment 2). The light diodes emitting in the region of 630±10 nm were used for plant irradiation. Other plants in three vessels of each line, which were not illuminated by the red light, were used as a control group. It was shown that under the short day the SD line in the control group passed to flowering 43±1.8 days after germination and PhPN line – 44±2.2 days after germination. The red-light irradiation, both before the beginning and in the middle of night, caused a delay of the transition to flowering in the SD line by 5±1 and 7±2.2 days, respectively. In the PhPN line, changing flowering period due to RL was not established. In the SD line, activation of the phytochromes by RL before the dark period caused an increase of the biomass, leaf area, total chlorophyll content, reduction of potassium ferrocyanide and photophosphorylation by isolated chloroplasts per chlorophyll of one leaf. While interruption of the night by RL caused decrease of these parameters and Hill reaction intensity per 1 mg of chlorophyll. The effect of RL on the studied parameters in the PhPN soybean line has not been detected.


2015 ◽  
Vol 2 (1) ◽  
pp. 632
Author(s):  
Kumala Dewi ◽  
Sudjino .

<p>Soybean (Glycine max L.) is one of horticultural plants in Indonesia. The demand of soybean in Indonesia is about 2,2 million ton per year, however, the productivity of soybean is still relatively low. This research was aimed to induce mutation in soybean through an application of sodium azide. Soybean seed of “Argomulyo” variety was obtained from Reserach Station for Leguminosae and Tuberous plant in Malang. Seeds were soaked in sodium azide of 0 mM (control), 5 mM, 10 mM or 20 mM for 12 hours. The pH of sodium azide solution was adjusted to 3 by adding sulphuric acid. For each treatment 100 seeds (M1) were used . Seeds were washed under tap water , germinated and grown in a polybag containing soil and compost vertilizer (3 : 1, v/v). In each polybag 4 seeds were germinated. All plants were watered regularly every other day. Growth and development parameters observed were plant height, chlorophyll content, time to flowering, sillique number per plant, seed dry weight and total protein content. Data were analyzed by Analysis of Variant and followed by DMRT (Duncan’s Multiple Range Test) at significant level of 5%. The results showed that sodium azide of 5 mM, 10 mM or 20 mM decreased the average of plant height 15 to 20% compared to control plants. Time to flowering, chlorophyll content, sillique number per plant, and seed dry weight were not affected by sodium azide. The average of total protein content in seeds of plant originated from seeds treated with sodium azide was 40% and this value is 5% higher compared to seeds of control plants. This seeds are potential to be further replanted and reselected for better characters.</p><p><br /><strong>Keywords</strong>: soybean (Glycine max L.), sodium azide, chlorophyll contenyt, total protein</p>


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