scholarly journals Myxobolus sp. (Myxozoa) in the circulating blood of Colossoma macropomum (Osteichthyes, Characidae)

2011 ◽  
Vol 20 (1) ◽  
pp. 82-84 ◽  
Author(s):  
Patricia Oliveira Maciel ◽  
Elizabeth Gusmão Affonso ◽  
Cheila de Lima Boijink ◽  
Marcos Tavares-Dias ◽  
Luis Antonio Kioshi Aoki Inoue
Keyword(s):  
Fish Species ◽  
Amazon Region ◽  
Colossoma Macropomum ◽  
First Report ◽  
Saltwater Fish ◽  
Fish Blood ◽  
Blood Smears ◽  
Myxosporean Species

Myxosporea parasitize many organs in fresh and saltwater fish. Species of the genus Myxobolus parasitizing the gills and other organs of the tambaqui Colossoma macropomum have been described. In the present study, blood smears were made from juvenile tambaqui and were stained with May Grunwald-Giemsa-Wright in order to identify myxozoan parasites. Out of a total of 36 fish examined, one specimen (2.7%) that was reared in a cage presented spores that were identified as M. colossomatis, whereas fish kept in 250 L tanks showed prevalence of 5.5%. This is the first report of M. colossomatis in the blood of farmed tambaqui in the Amazon region. These results indicate that myxozoan parasites should also be investigated in fish blood smears. Some myxosporean species may cause diseases in fish, and these species need to be identified so that adequate preventive sanitary control can be instituted.

scholarly journals First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

2011 ◽  
Vol 20 (1) ◽  
pp. 85-87 ◽  
Author(s):  
Santiago Benites de Pádua ◽  
Márcia Mayumi Ishikawa ◽  
Fabiana Satake ◽  
Gabriela Tomas Jerônimo ◽  
Fabiana Pilarski
Keyword(s):  
Prevalence Rate ◽  
First Record ◽  
Diagnostic Methods ◽  
Mato Grosso ◽  
First Report ◽  
Paraguay River ◽  
Blood Smears ◽  
Mato Grosso Do Sul ◽  
Pantanal Wetland ◽  
Blood Centrifugation

The blood infection by Trypanosoma sp. in tuvira (Gymnotus aff. inaequilabiatus) from the Pantanal wetland was reported in this study. Ten fish from the Paraguay River in the Pantanal were evaluated for the presence of hemoflagellates. Trypomastigotes of Trypanosoma sp. were observed in blood smears from three fish (30% prevalence) and some forms were seen to be undergoing division. Using the diagnostic methods of fresh examination and blood centrifugation in hematocrit capillary tubes, the prevalence rate was 80%. This is the first report of Trypanosoma sp. in tuvira in Brazil.

Composition of peripheral blood leukocytes and immunocompetent organs of white char in the lake Kronotskoye

2021 ◽  
pp. 42-49
Author(s):  
Ilya Ivanovich Gordeev Ilya Ivanovich Gordeev
Keyword(s):  
Fish Species ◽  
Peripheral Blood ◽  
White Blood Cells ◽  
Head Kidney ◽  
Stress Factors ◽  
Lake Basin ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Immunocompetent Organs ◽  
Blood Smears

The composition of leukocytes reflects the species features of fish. The ratio of individual cell types reflects the functional state of the organism and the nature of the influence of biotic and abiotic stress factors. Kronotskoye Lake is located in the Far East on the territory of the Kronotsky State Natural Biosphere Reserve (Kamchatka). Due to the peculiarities of the hydrological regime and the relief of the Kronotskaya River basin, anadromous migration along it is limited. Therefore, anadromous fish do not return to the lake after the marine period of their life cycle. The lake basin is inhabited by at least six reproductively isolated forms (species) of chars. White char, Salvelinus albus Glubokovsky, 1977, at the early stages of ontogenesis feeds on invertebrates, and as it grows up, it goes on to a predatory lifestyle. White char migrates around the lake: spawning occurs in the tributaries, and feeding takes place in the lake itself. This work is devoted to the study of the intensity of leukopoiesis and leukocyte composition of the blood, head kidney and spleen of the white char of Lake Kronotskoye. Seven individuals caught using gill nets from July 8 to August 14, 2011 were studied. Peripheral blood was sampled from the tail vein. Sampling also included spleen and head kidney tissues, blood smears and organ-imprinted smears of kidney and spleen. The leukocyte formula and the frequency of occurrence of leukocytes in a peripheral blood smears were calculated using light microscope. The composition of white blood cells in the white char is similar to that of other fish species (lymphocytes, monocytes, neutrophils, and blast cell forms). No basophils or eosinophils were found. In organ-imprinted smears of immunocompetent organs (kidney, spleen), the percentage of lymphocytes is lower and the percentage of blast forms is higher than in the blood leukogram. The abundance index differed from the data obtained in the study of other fish species. English version of the article is available at URL: https://panor.ru/articles/composition-of-peripheral-blood-leukocytes-and-immunocompetent-organs-of-the-white-char-of-lake-kronotskoye-kamchatka/68317.html

scholarly journals The improvement of blood smears coloring for the determining of fish cytometric blood parameters

2019 ◽  
Vol 11 (1) ◽  
pp. 15-18
Author(s):  
V Kurchenko ◽  
T Sharamok ◽  
O Marenkov
Keyword(s):  
Blood Cells ◽  
Morphological Changes ◽  
Blood Parameters ◽  
Field Conditions ◽  
Improved Method ◽  
Fish Blood ◽  
Blood Smears ◽  
Correct Form ◽  
The Subject

Fish erythrocytes are sensitive to environmental pollution and their morphological changes can be used as a bioindicator of the toxicity of the aquatic environment. The disadvantage of existing methods of blood smears coloring is that they are inconvenient in the field conditions, when it is necessary to examine the fish blood during ichthyological researches or they are long-lasting. In this regard, there is a need for a fast and reliable method of coloring blood cells of fish, which can be used both in laboratory and in field conditions. The aim of the work was to improve the method of coloring fish blood smears for easier and faster execution, without degradation of the quality of colouring. The subject of the research was the young Cyprinidae fish. The improved method is based on the classic method of painting by Papenheim, but differs in the interval of time devoted to colouring in a solution of the dye Romanovskogo. The analysis showed that erythrocytes on medicine that were in the Romanovsky solution for 5 minutes had the correct form with clear contours, a dark purple core located in the center of the cell. It has been found that this method can be used in cytometric researches to determine such indicators: large longitudinal and small transverse diameters of erythrocytes, erythrocyte area, erythrocyte core area, nuclear-cytoplasmic ratio, percentage of mature and immature erythrocytes and their forms. It can also be used in analyzing the presence of erythrocyte pathologies. It has been established that reduction of time (from 20 minutes to 5 minutes) of preparations in the dye solution of Romanovsky does not affect the quality of their coloring, and therefore it is a reliable and fast way that can be successfully applied in morphological and cytometric studies of blood of fish.

scholarly journals Infection by Trypanosoma spp. in Platydoras armatulus (Siluriformes, Doradidae), in Southwestern Amazon, Brazil

2021 ◽  
Author(s):  
Lucena Rocha Virgilio ◽  
Gabriele Oliveira Texeira ◽  
Henrique Paulo Silva De Melo ◽  
Sérgio Luiz Prolo Junior ◽  
Ricardo Massato Takemoto ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Infection Intensity ◽  
Morphometric Measurements ◽  
Blood Samples ◽  
First Report ◽  
Cardiac Puncture ◽  
Fish Blood

Abstract Trypanosoma is a hemoflagellate capable of infecting a wide variety of invertebrates and vertebrates, such as Neotropical freshwater fish. Thus, the present study described and morphologically compared Trypanosoma sp., found in Platydoras armatulus, Valenciennes, 1840, in southwestern Amazon. A sampling of fish specimens was carried out in a river located in Guajará, Amazonas, Brazil. Fish blood samples were collected through a cardiac puncture. Thus, smears were made for quantification, morphometric measurements, and morphotyping of trypanosomes found. Prevalence, mean abundance, and intensity of parasitism were estimated in the specimens of parasitized fish. Five fish specimens were collected, showing a 100% prevalence of parasites in the host. We found two Trypanosoma morphotypes, A and B, in which A had the highest infection intensity in host specimens. Thus, the present study showed the first report of Trypanosoma spp. in P. armatulus, besides the probability of two parasitic morphospecies in the blood of these fish specimens.

scholarly journals Mollusks of the genus Biomphalaria Preston, 1910 in Amazon Region: first report of Biomphalaria occidentalis Paraense, 1981 in Pará State, Brazil

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Christiane de Oliveira Goveia ◽  
Roberta Lima Caldeira ◽  
Márcio Roberto Teixeira Nunes ◽  
Martin Johannes Enk
Keyword(s):  
Amazon Region ◽  
First Report

scholarly journals Aristolactams from roots of Ottonia Anisum (Piperaceae)

2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600
Author(s):  
André M. Marques ◽  
Leosvaldo S. M. Velozo ◽  
Davyson de L. Moreira ◽  
Elsie F. Guimarães ◽  
Maria Auxiliadora C. Kaplan
Keyword(s):  
Atlantic Forest ◽  
Biological Activities ◽  
Chemical Compounds ◽  
Amazon Region ◽  
First Report ◽  
H Nmr ◽  
Medicinal Properties

The Piperaceae species are known worldwide for its medicinal properties and its chemical compounds. In Brazil, many species of this family are distributed mainly in Amazon Region and in the Atlantic Forest. The genus Ottonia is known as source of amides, flavonoids, arylpropanoids and terpenes with record biological activities. Six aristolactams, including, aristolactam BII, piperolactam C, goniothalactam, stigmalactam, aristolactam AII and aristolactam BIII were isolated from roots of this species. GC-MS, 1H NMR and NOESY techniques were used to characterize these compounds. This is the first report about the occurrence of aristolactams in the Ottonia anisum Sprengel.

First report of Paracoccidioides brasiliensis infection in fish

2019 ◽  
Vol 58 (6) ◽  
pp. 737-743
Author(s):  
Igor Massahiro de Souza Suguiura ◽  
Rafaela Macagnan ◽  
Aline Myuki Omori ◽  
Elder Luis Buck ◽  
Josiane Aniele Scarpassa ◽  
...  
Keyword(s):  
Latin American ◽  
Fish Species ◽  
Nile Tilapia ◽  
Paracoccidioides Brasiliensis ◽  
Aquatic Organisms ◽  
Yeast Cells ◽  
Molecular Evidence ◽  
Serum Samples ◽  
Tissue Samples ◽  
First Report

Abstract The thermodimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis (PCM), a deep mycosis endemic in Latin American countries that affects mainly male rural workers. Infection by P. brasiliensis has also been reported in several species of terrestrial animals; however, the capacity of the fungus to infect aquatic organisms is poorly known. The aim of this study was to detect P. brasiliensis in a fish species, Nile tilapia (Oreochromis niloticus), the most farmed and widely distributed fish in endemic areas for human PCM in Brazil. As a first step, the humoral immune response against the fungus was evaluated in an experimental group of three fish immunized with inactivated P. brasiliensis yeast cells. For the seroepidemiological study, serum samples of Nile tilapia raised in cages (n = 109) and in ponds (n = 105), collected from a fish slaughterhouse, were analyzed for P. brasiliensis antibodies by ELISA using gp43 as antigen. All the inoculated fish produced antibodies against the fungus. The seropositivity observed in fish raised in cages and ponds was 17.4 and 5.7%, respectively. Due to the higher seropositivity observed in caged fish, 100 tissue samples (encephalon, liver, and kidney), from another group of tilapia raised in cages, were analyzed by polymerase chain reaction (PCR; Pb-ITSR and Pb-ITSE). Three tissue samples (liver n = 1, kidney n = 1, and enchepahlon n = 1) from three different fish resulted positive to PCR. This is the first report to show serological and molecular evidence of P. brasiliensis infection in a fish species.

scholarly journals An outbreak of myxozoan parasites in farmed freshwater fish Colossoma macropomum (Cuvier, 1818) (Characidae, Serrasalminae) in the Amazon region, Brazil

2016 ◽  
Vol 3 ◽  
pp. 31-34 ◽  
Author(s):  
Marcela Videira ◽  
Michele Velasco ◽  
Cinthia Silva Malcher ◽  
Patrícia Santos ◽  
Patrícia Matos ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Amazon Region ◽  
Colossoma Macropomum

scholarly journals A fast and practical method for initial diagnosis of Piscinoodinium pillulare outbreaks: piscinootest

2018 ◽  
Vol 48 (7) ◽  
Author(s):  
Ana Lucia Silva Gomes ◽  
Jesaías Ismael da Costa ◽  
Maria Linda Flora de Novaes Benetton ◽  
Geraldo Bernardino ◽  
Andrea Belem-Costa
Keyword(s):  
Geographical Distribution ◽  
Practical Method ◽  
Amazon Region ◽  
Initial Diagnosis ◽  
Fish Farms ◽  
Colossoma Macropomum ◽  
Precision Equipment

ABSTRACT: The dinoflagellate Piscinoodinium pillulare is a parasite with low parasitic specificity and unspecific geographical distribution. This research reported a simple and practical method - the principle of spontaneous sedimentation - to identify the presence of P. pillulare during massive infestations which does not require using precision equipment. Agitation in water of the gill arches of infested fish will elicit, after a period of rest, recording the accumulation of trophonts in the bottom of flasks. The method was applied during outbreaks of P. pillulare in tambaqui, Colossoma macropomum and matrinxã, Brycon amazonicus, in fish farms of the Amazon region and proved to be effective.

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