Pharmacobotanic characterization of young stems and stem barks of Rauvolfia sellowii Müll. Arg., Apocynaceae

Rauvolfia sellowii Müll. Arg. (Apocynaceae), a Brazilian native tree rich in indole alkaloids, is known as "pau-pra-tudo" and popularly used as hypocholesterolemic, hypoglycemic and antihypertensive. The aim of this work was to study the anatomy of the young stems and stem barks of this medicinal plant, in order to contribute to the identification of the species as a drug. The plant material was fixed and prepared according to standard microtechniques. The young stems have remaining epidermis, but a suberified peridermis is evident. The phellogen is located in the cortical region, forming suber externally. Underneath the phellogen, lies the phelloderm and collenchymatic region. In the cortex, there are numerous laticifers and some fibers. There is an incomplete sclerenchymatic sheath, consisting of several groups of fibers and stone cells. The stem has internal phloem ordered as isolated groups side by side. Numerous laticifers, calcium oxalate crystals, idioblasts and amyloplasts are found in the cortex, phloem, xylem and pith. The stem bark has many layers of suber and cortical parenchyma, a sheath composed of fibers and stone cells totally lignified, and external phloem. These anatomical characteristic, taken together, can be used as quality control parameters for this species.

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Micromorphological and Pharmacognostic Studies of Leaf and Stem of Solenostemon monostachyus P. Beauv (Lamiaceae)

Journal of Complementary and Alternative Medical Research ◽

10.9734/jocamr/2021/v16i430312 ◽

2021 ◽

pp. 230-240

Author(s):

Romanus A. Umoh ◽

Imoh I. Johnny ◽

Anwanabasi E. Udoh ◽

Nsima A. Andy ◽

Affiong C. Essien ◽

...

Keyword(s):

Water Soluble ◽

Control Parameters ◽

Plant Leaves ◽

Calcium Oxalate Crystals ◽

Total Acid ◽

Drug Products ◽

Standard Procedures ◽

Quality Control Parameters ◽

Microscopic Features ◽

Microscopic Studies

Solenostemon monostachyus P. Beauv (Lamiaceae), it’s ethnomedical uses include anti-plasmodial, anti-pyretic, antiulcerogenic, antioxidant, anti-inflammatory and anti-nociceptive activities. The aim of this study was to employ the quality control parameters in the evaluation of the leaf and stem of Solenostemon monostachyus to aid in the identification and standardization of the medicinal plant. The plant leaves and stems were collected, identified, air-dried, pulverized and stored in separate glass containers. Standard procedures were employed to obtain the microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, fluorescence properties, moisture contents, ash values and soluble extractive values. The results of the microscopic studies using fresh and powdered leaf samples revealed the presence of diacytic stomata on both the abaxial and adaxial surfaces (amphistomatic), with stomatal index of 27.9% and 14.8% respectively. The result of the micromeritics properties of the powdered leaf and stem samples showed angles of repose of 38.0o and 46.0o, Carr’s index of 23.7% and 32.5% and Hausner’s ratios of 1.3 and 1.5 respectively. Results for the moisture content, total, acid-insoluble and water-soluble ash values were 11.7%w/w, 13.7%w/w, 1.8%w/w and 9.4%w/w for the leaf and 13.3%w/w, 17.3%w/w, 1.8%w/w and 9.2%w/w for the stem respectively. Extractive values for water-soluble, methanol-soluble and ethanol-soluble were 26.5%w/w, 32.3%w/w, 14.5%w/w and 15.5%w/w, 15.8%w/w and 14.5%w/w for the leaf and stem respectively. Chemomicroscopy indicated the presence of lignin, calcium oxalate crystals and protein in the leaf and stem. The results obtained therefore could be used to establish pharmacopoeial standard for the fresh and powdered drug products of Solenostemon monostachyus, thus preventing adulteration.

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Extraction, Identification and Pharmacological Evaluation of Phyto-Active Compound in Manilkara Hexandra (Roxb.) Dubard Stem Bark

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2677 ◽

2018 ◽

Vol 15 (3) ◽

pp. 687-698 ◽

Cited By ~ 1

Author(s):

S. Irudaya Monisha ◽

J. Rosaline Vimala

Keyword(s):

Preliminary Analysis ◽

Plant Material ◽

Stem Bark ◽

Active Principle ◽

Lead Compound ◽

Mineral Analysis ◽

Zone Of Inhibition ◽

High Zone ◽

Ft Ir

Plants are made up of chemicals of differing nature produced by metabolism. These phytochemicals show varied biological activity. The present work was aimed to identify such active principle present in the stem bark of Manilkara hexandra (Roxb.)Dubard. Proximate, fluorescence, histochemical, mineral analysis was carried out for the plant material. From the preliminary analysis, the flavonoid rich extract was identified which was then subjected to using chromatographic methods for compound separation. Characterization of the separated compound was done by UV, FT-IR, and GC-MS studies. 7,9- DI-TERT-BUTYL-1-OXASPIRO[4.5]DECA-6,9-DIENE-2,8-DIONE was identified by GC-MS analysis. Antibacterial activity was carried and it shows high zone of inhibition, when concentration increases from 30 to 150 μg/ml. The separated flavanoid in future could be used to study its wide pharmacological applications as a lead compound in drug delivery.

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Characterization of stilbenoids from the stem bark of Norway spruce (Picea abies) as antifungal agents

Planta Medica ◽

10.1055/s-0036-1596789 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

J Gabaston ◽

T Richard ◽

B Biais ◽

P Waffo-Teguo ◽

MF Corio-Costet ◽

...

Keyword(s):

Picea Abies ◽

Norway Spruce ◽

Antifungal Agents ◽

Stem Bark

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Quality Control Parameters of Mandukarni Syrup - A classical memory enhancing dosage form

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v2i4.9328 ◽

2017 ◽

Vol 2 (4) ◽

Author(s):

Almas Tarannum ◽

Prathviraj Puranik ◽

Suma V. Mallya

Keyword(s):

Quality Control ◽

Refractive Index ◽

Specific Gravity ◽

Dosage Form ◽

Reducing Sugar ◽

Standard Protocol ◽

Direct Impact ◽

Control Parameters ◽

Quality Control Parameters ◽

Memory Enhancing

Ayurveda is the healing medicine. Dosha, Dhatu, Mala and Agni play important role in maintaining health of a person, but without equilibrium of Atma, Indriya and Manas they are helpless. Manas is one entity which is the controller of health. There are several herbs which have direct impact on Manas, among them Mandukaparni is one. Swarasa of Mandukaparni is highly effective, but it is not easily available for children. This study is intended to make Syrup form of Mandukaparni and evaluate its pharmacognostical parameters. According to the methodology refractive index, total solids, specific gravity, reducing and non Reducing sugar and HPTLC parameters were assessed. The results were found to be genuine fulfilling the standard protocol. This study is under taken to evaluate the pharmacognostic properties of Mandukaparni syrup.

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Analytical profile of Arka Taila - An Ayurvedic oil based medicine used in Karnasrava (Otomycosis)

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v2i3.8212 ◽

2017 ◽

Vol 2 (3) ◽

Author(s):

Komal D. Jani ◽

Dharmendra P. Jani

Keyword(s):

Quality Control ◽

Fungal Infections ◽

Curcuma Longa ◽

Calotropis Procera ◽

Control Parameters ◽

Scientific Evaluation ◽

Quality Control Parameters ◽

Quality Check ◽

Based Medicine ◽

Excessive Accumulation

Otomycosis denotes diffuse otitis externa due to fungal infections. The fungii are usually secondary invaders of the tissue rendered susceptible by bacterial infection, physical injury or excessive accumulation of cerumen in the external auditory canal. Karnasraava means discharge from ear which can be correlated with otorrhoea. Karnasraava (discharge) is one of symptom of otomycosis. Arka Taila has been mentioned in Shaarangadhara is very simple formula having ingredients i.e. Haridraa (Curcuma longa), Arka Patra Swarasa (Calotropis procera) and Sarshapa Taila (Brassica campetries). The quality control parameters resulted after scientific evaluation of Arka Taila can be used as reference standard for quality control or quality assurance of a pharmaceutical industry in order to have a proper quality check over its preparation and processing.

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[18F]F-PSMA-1007 Radiolabelling without an On-Site Cyclotron: A Quality Issue

Pharmaceuticals ◽

10.3390/ph14070599 ◽

2021 ◽

Vol 14 (7) ◽

pp. 599

Author(s):

Valentina Di Iorio ◽

Stefano Boschi ◽

Anna Sarnelli ◽

Cristina Cuni ◽

David Bianchini ◽

...

Keyword(s):

Quality Assurance ◽

Radiochemical Purity ◽

Competent Authority ◽

Radiochemical Yield ◽

Synthesis Process ◽

Control Parameters ◽

Quality Issue ◽

Assurance System ◽

Quality Control Parameters ◽

Specific Membrane

Radiopharmaceuticals targeting the prostate-specific membrane antigen (PSMA) has become the gold standard for PET imaging of prostate cancer. [68Ga]Ga-PSMA-11 has been the forerunner but a [18F]F-PSMA ligand has been developed because of the intrinsic advantages of Fluorine-18. Fluorine-18 labelled compounds are usually prepared in centers with an on-site cyclotron. Since our center has not an on-site cyclotron, we decided to verify the feasibility of producing the experimental 18F-labelled radiopharmaceutical [18F]F-PSMA-1007 with [18F]F- from different external suppliers. A quality agreement has been signed with two different suppliers, and a well-established and correctly implemented quality assurance protocol has been followed. The [18F]F- was produced with cyclotrons, on Nb target, but with different beam energy and current. Extensive validation of the [18F]F-PSMA-1007 synthesis process has been performed. The aim of this paper was the description of all the quality documentation which allowed the submission and approval of the Investigational Medicinal Product Dossier (IMPD) to the Competent Authority, addressing the quality problems due to different external suppliers. The result indicates that no significant differences have been found between the [18F]F- from the two suppliers in terms of radionuclidic and radiochemical purity and [18F]F- impacted neither the radiochemical yield of the labelling reaction nor the quality control parameters of the IMP [18F]F-PSMA-1007. These results prove how a correct quality assurance system can overcome some Regulatory Authorities issue that may represent an obstacle to the clinical use of F-18-labelled radiopharmaceuticals without an on-site cyclotron

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Isolation and characterization of aristolactam alkaloids from the stem bark of Goniothalamus velutinus (Airy Shaw) and their biological activities

Journal of King Saud University - Science ◽

10.1016/j.jksus.2016.12.008 ◽

2018 ◽

Vol 30 (1) ◽

pp. 41-48 ◽

Cited By ~ 6

Author(s):

Erum Iqbal ◽

Linda B.L. Lim ◽

Kamariah Abu Salim ◽

Shaheen Faizi ◽

Ayaz Ahmed ◽

...

Keyword(s):

Biological Activities ◽

Stem Bark ◽

Isolation And Characterization

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Action ofCoriandrum sativumL. Essential Oil upon OralCandida albicansBiofilm Formation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/985832 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 38

Author(s):

V. F. Furletti ◽

I. P. Teixeira ◽

G. Obando-Pereda ◽

R. C. Mardegan ◽

A. Sartoratto ◽

...

Keyword(s):

Essential Oil ◽

Biofilm Formation ◽

Plant Material ◽

Inhibitory Concentration ◽

Chemical Characterization ◽

Synergistic Action ◽

Biofilm Growth ◽

Water Distillation ◽

Microdilution Method

The efficacy of extracts and essential oils fromAllium tuberosum, Coriandrum sativum, Cymbopogon martini, Cymbopogon winterianus,andSantolina chamaecyparissuswas evaluated againstCandidaspp. isolates from the oral cavity of patients with periodontal disease. The most active oil was fractionated and tested againstC. albicansbiofilm formation. The oils were obtained by water-distillation and the extracts were prepared with macerated dried plant material. The Minimal Inhibitory Concentration—MIC was determined by the microdilution method. Chemical characterization of oil constituents was performed using Gas Chromatography and Mass Spectrometry (GC-MS). C. sativum activity oil upon cell and biofilm morphology was evaluated by Scanning Electron Microscopy (SEM). The best activities against planktonicCandidaspp. were observed for the essential oil and the grouped F8–10fractions fromC. sativum. The crude oil also affected the biofilm formation inC. albicanscausing a decrease in the biofilm growth. Chemical analysis of the F8–10fractions detected as major active compounds, 2-hexen-1-ol, 3-hexen-1-ol and cyclodecane. Standards of these compounds tested grouped provided a stronger activity than the oil suggesting a synergistic action from the major oil constituents. The activity ofC. sativumoil demonstrates its potential for a new natural antifungal formulation.

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