<p>ABSTRAK<br />Helopeltis antonii merupakan salah satu hama pada tanaman kakao,<br />teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkan<br />stiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.<br />Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum<br />(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfa<br />keturunan, persistensi terhadap mortalitas dan oviposisi H. antonii.<br />Penelitian dilaksanakan di Laboratorium Fisiologi dan Toksikologi<br />Serangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,<br />Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei<br />2013 sampai April 2014. Buah mentimun digunakan sebagai inang<br />pengganti untuk perbanyakan serangga uji H. antonii di laboratorium dan<br />media pengujian. Hasil penelitian menunjukkan kematian imago H.<br />antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadi<br />pada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSP<br />terjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanya<br />terjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.<br />retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfa<br />keturunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum<br />0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%<br />(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar matahari<br />hingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidak<br />efektif dalam menghambat peletakan telur H. antonii. Penghambatan<br />peletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.<br />retrofractum 0,98% (2 × LC 95 ) yang dipajankan di bawah sinar matahari<br />selama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan<br />23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untuk<br />menilai kelayakan dalam pengendalian H. antonii.<br />Kata kunci: insektisida botani, mortalitas, oviposisi, persistensi</p><p>ABSTRACT<br />Helopeltis antonii is cocoa, tea, and cashew nuts important pest that<br />causes damage by sucking plant sap from shoots and nuts. This study was<br />conducted to test toxicity of Piper retrofractum fruit extract on adults,<br />sublethal effect on the production nymphal progeny, and persistence<br />against mortality and oviposition of H. antonii. This study was conducted<br />at The Fisiology and Insect Toxicology Laboratory, Plant Protection<br />Departement, Bogor Agricultural University and The Plant Protection<br />Laboratory of Indonesian Industrial and Beverage Crops Research Institute<br />from Mey 2013 to April 2014. Cucumber was used as a host substitute for<br />rearing the test insect and as the testing medium. The results show that<br />adult mortality, due to the P. retrofractum leaf extract treatment 0.05-<br />0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.<br />antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only a<br />slight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractum<br />extract at 120 HAT were 0.20 and 0.49%, respectively. The treatment at<br />sublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphal<br />progeny number. P. retrofractum extract suspensions at LC 95 and 2 × LC 95<br />exposed under sunlight for 5 days were still effective against H. antonii<br />adults (80% mortality), but were not effective in females inhibiting<br />oviposition. The oviposition inhibiting activity was observed only in the<br />treatment with P. retrofractum extract at 2 × LC 95 exposed under sunlight<br />for 0 and 1 day in which the oviposition deterrency indices were 22.7 and<br />23.8% respectively. <br />Key words: botanical insecticides, mortality, oviposition, persistence</p>
Toxicity and Sublethal Effect of Farnesyl Acetate on Diamondback Moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)
