Effects of the uterus and the ovaries on growth, somatotrophin and somatomedin A in developing rats
ABSTRACT The effects of hysterectomy and ovariectomy on plasma concentrations of GH, somatomedin A, TSH and thyroxine (T4) were studied in developing rats. Four groups of 24-day-old rats were ovariectomized, ovohysterectomized, hysterectomized or sham-operated. Their weights, lengths and plasma hormone concentrations were measured at 26, 43, 64, 78 and 92 days of age to investigate pre- and postpubertal differences caused by the uterus or ovaries. Plasma concentrations of the hormones examined showed a successive rise with time, but GH and somatomedin A concentrations rose mainly after the opening of the vagina (days 50–55). Higher GH and somatomedin A concentrations were found in the plasma of ovariectomized animals than in ovohysterectomized controls before puberty (GH: 260–300%, P<0·01; somatomedin A: 25–30%, P<0·05). Ovariectomized animals weighed more than ovohysterectomized females after puberty (4·5–6%, P<0·01). This indicated that the uterus exerted a stimulatory effect on GH-somatomedin A regulation and body weight gain in the absence of the ovaries. Significantly lower plasma somatomedin A (but not GH) concentrations were found in hysterectomized and sham-operated animals than in their respective controls after puberty (30–39%, P<0·01) and their final body weight was lower (22–26%, P<0·001). There were no consecutive uterus- or ovary-related changes in plasma TSH and T4 levels. It was concluded that both the uterus and ovaries had significant as well as opposite effects on somatomedin A and body weight with the effects of the ovaries being greater than those of the uterus. J. Endocr. (1987) 113, 21–26