Effect of thyroidectomy, and thyroxine and α2u-globulin replacement therapy on testicular steroidogenic and gametogenic activities in rats

1994 ◽  
Vol 140 (3) ◽  
pp. 343-347 ◽  
Author(s):  
N M Biswas ◽  
P K Ghosh ◽  
R Biswas ◽  
D Ghosh
Keyword(s):  
Body Weight ◽  
Thyroid Hormone ◽  
Replacement Therapy ◽  
Serum Levels ◽  
Male Rats ◽  
Testicular Function ◽  
Serum Concentrations ◽  
Hydroxysteroid Dehydrogenases ◽  
Accessory Sex Organs ◽  
Hormone Control

Abstract Adult male rats were thyroidectomized and killed after 22 days of treatment. Thyroidectomy lowered the weights of testes and accessory sex organs, decreased the activities of testicular Δ5-3β- and 17β-hydroxysteroid dehydrogenases (HSD), and diminished spermatogenesis, serum levels of testosterone and α2u-globulin. Supplementation with thyroxine at a dose of 5 μg/100 g body weight per day for 21 days or supplementation with α2u-globulin at a dose of 1·5 mg/rat per day for 21 days in thyroidectomized animals partially reversed the decrease in HSD activities and serum concentrations of testosterone and α2u-globulin, while spermatogenesis was restored to normal. The weights of testes and accessory sex organs were also reinstated after supplementation with thyroxine or α2u-globulin in thyroidectomized rats in comparison with thyroidectomized animals. It was concluded that α2u-globulin may be an intermediary in the thyroid hormone control of testicular function. Journal of Endocrinology (1994) 140, 343–347

Effect of dihydrotestosterone on serum concentrations of α2u-globulin and on spermatogenesis in melatonin-treated rats

1990 ◽  
Vol 126 (3) ◽  
pp. 431-435 ◽  
Author(s):  
H. Mandal ◽  
P. K. Ghosh ◽  
N. M. Biswas
Keyword(s):  
Body Weight ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Male Rats ◽  
Testicular Function ◽  
Serum Concentrations ◽  
Melatonin Treatment ◽  
Accessory Sex Organs

ABSTRACT Adult male rats were given s.c. injections of melatonin (400 μg/100 g body weight per day) for 14 days. On day 15, the weights of the testis and accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase (17β-HSD) activity was inhibited, spermatogenesis was suppressed and serum levels of gonadotrophins, testosterone and α2u-globulin were decreased compared with control animals injected with vehicle. In a third group of rats given the same dose of melatonin for 14 days, administration of dihydrotestosterone (DHT) at a dose of 25 μg/100 g body weight per day on days 8–14 resulted in serum levels of α2u-globulin, FSH, LH and testosterone and testicular 17β-HSD activity similar to those seen in vehicle-injected control animals. Weights of the testes and accessory sex organs and spermatogenesis were normal after administration of DHT in melatonintreated rats. In another group of rats, the depressive effects of melatonin treatment on plasma gonadotrophins were reversed by the administration of α2u-globulin on days 8–14. It was concluded that treatment with DHT prevents the depressive action of melatonin on testicular function by inducing the synthesis of α2u-globulin. Journal of Endocrinology (1990) 126, 431–435

Effect of α2u-globulin on serum concentration of gonadotrophins and testicular activity in oestrogen-treated rats

1983 ◽  
Vol 96 (2) ◽  
pp. 321-327 ◽  
Author(s):  
N. M. Biswas ◽  
P. K. Ghosh ◽  
K. K. Ghosh ◽  
O. W. Neuhaus
Keyword(s):  
Serum Concentration ◽  
Adult Male ◽  
Serum Levels ◽  
Hydroxysteroid Dehydrogenase ◽  
Organ Weight ◽  
Male Rats ◽  
Testicular Function ◽  
Accessory Sex Organs ◽  
Testosterone Synthesis

Adult male rats were given injections of oestradiol-17β (50 μg/100 g body wt per day) for 7 days. When they were killed 14 days after the last injection, serum levels of gonadotrophins and testosterone and weights of accessory sex organs were less, testicular 17β-hydroxysteroid dehydrogenase 17β-HSD activity was suppressed and spermatogenesis was inhibited. Administration of α2u-globulin (1·5 mg/day) for 14 days to oestrogen-treated rats and for 10 days to control rats resulted in increased concentrations of gonadotrophins and testosterone in the serum. Accessory sex organ weight and spermatogenesis appeared to be normal while 17β-HSD activity increased in oestrogen-treated rats after treatment with α2u-globulin. It was concluded that α2u-globulin has an effect on testicular function in oestrogenized rats by inducing gonadotrophin and testosterone synthesis.

GONADOTROPHIN SUPPRESSION BY STEROIDS IN NORMAL, ADULT MALE RATS

1977 ◽  
Vol 84 (4) ◽  
pp. 842-849 ◽  
Author(s):  
H. L. Verjans ◽  
K. B. Eik-Nes
Keyword(s):  
Body Weight ◽  
High Activity ◽  
Adult Male ◽  
Subcutaneous Administration ◽  
Serum Levels ◽  
Steroid Treatment ◽  
Male Rats ◽  
Normal Adult ◽  
Serum Lh ◽  
Testosterone Metabolites

ABSTRACT Effects of subcutaneous administration of various doses of different steroids on serum levels of LH and FSH in normal, adult male rats were investigated. Each steroid was injected daily during 7 days in doses of 50, 25, 12.5 or 6.25 μg per 100 g body weight. Ring A 5α-reduced testosterone metabolites exerted high activity in suppressing serum LH and FSH levels. 5α-Androstane-3β,17β-diol had in the doses tested, however, no such effects on serum gonadotrophins. Serum LH concentrations in normal rats appeared more sensitive to steroid treatment than serum FSH concentrations.

The contribution of corticosterone and testosterone to the suppression of serum LH in hyperprolactinaemic adult male rats with pituitary transplants

1987 ◽  
Vol 113 (1) ◽  
pp. 111-116 ◽  
Author(s):  
R. F. A. Weber ◽  
M. P. Ooms ◽  
J. T. M. Vreeburg
Keyword(s):  
Adult Male ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Male Rats ◽  
Male Rat ◽  
Serum Concentrations ◽  
Testosterone Secretion ◽  
Accessory Sex Glands ◽  
Serum Lh ◽  
Stimulation Of

ABSTRACT The effects of hyperprolactinaemia on serum levels of LH were investigated in adult male rats of the R × U strain. Hyperprolactinaemia was induced by three pituitary grafts under the kidney capsule, transplanted on day 0 of each experiment. Special attention was paid to the contribution of prolactin-stimulated testes, adrenals and corticosterone. In experiment 1, hyperprolactinaemia significantly reduced the serum concentrations of LH in intact rats. In spite of a significant increase in the serum levels of corticosterone, serum testosterone was not significantly affected by hyperprolactinaemia. The weights of both the adrenals and accessory sex glands were significantly increased at autopsy. In experiment 2, treatment with 10 mg corticosterone s.c. daily from day 14 to day 28 after pituitary grafting significantly reduced serum levels of both LH and testosterone. The suppression of testosterone in the hyperprolactinaemic corticosterone-treated animals was significantly less than in the corticosterone-treated control animals. The weights of the accessory sex glands were significantly increased in the hyperprolactinaemic animals. In experiment 3, rats were adrenalectomized and half of them were substituted with corticosterone. Serum testosterone levels significantly increased in both hyperprolactinaemic adrenalectomized rats and in adrenalectomized corticosterone-treated animals without any significant effect on serum LH. Again the weights of the accessory sex glands were significantly increased in the hyperprolactinaemic animals. In experiment 4, rats were adrenalectomized, gonadectomized and corticosterone treated on day 0 and then implanted with a 2, 1·5 or 1 cm silicone elastomer capsule containing testosterone. On day 28 after pituitary grafting, LH levels were significantly suppressed in animals with a 2 or 1·5 cm testosterone implant. The weights of the accessory sex glands were not increased in the hyperprolactinaemic animals. These results show that in the male rat the inhibitory effects of hyperprolactinaemia on serum LH levels may be due to (1) increased sensitivity of the hypothalamic-pituitary axis to the negative feedback action of testosterone by prolactin and by the prolactin-stimulated corticosterone secretion and (2) stimulation of testicular testosterone secretion by prolactin, which can also explain the increased weights of the accessory sex glands. Even in the presence of high serum concentrations of corticosterone, stimulation of testicular testosterone secretion by prolactin was observed. J. Endocr. (1987) 113,111–116

Plasma growth hormone pattern and androgens influence the levels of corticosteroid-binding globulin in rat serum

1989 ◽  
Vol 122 (3) ◽  
pp. 725-732 ◽  
Author(s):  
J.-O. Jansson ◽  
J. Oscarsson ◽  
A. Mode ◽  
E. M. Ritzén
Keyword(s):  
Serum Concentration ◽  
Replacement Therapy ◽  
Polyacrylamide Gel Electrophoresis ◽  
Female Rats ◽  
Male Rats ◽  
Testosterone Replacement Therapy ◽  
Serum Concentrations ◽  
Oestrogen Treatment ◽  
Corticosteroid Binding Globulin ◽  
Hypophysectomized Rats

ABSTRACT The serum concentration of corticosteroid-binding globulin (CBG) is higher in female rats than in males. Combined hypophysectomy and gonadectomy of female rats reduced the serum concentration of CBG as measured by steady-state polyacrylamide gel electrophoresis, whereas hypophysectomy of male rats increased serum CBG. These effects were seen despite replacement therapy with thyroxine and glucocorticoids. Moreover, neither androgen nor oestrogen treatment affected the serum concentrations of CBG in hypophysectomized rats. Continuous infusions of human or bovine GH (1·4 U/kg per day), by means of osmotic minipumps for 1 week, increased serum concentrations of CBG in both hypophysectomized male and female rats. In contrast, intermittent GH replacement therapy by s.c. injections at 12-h intervals either had no effect or suppressed serum CBG levels. In male rats, neonatal (days 1–2) gonadectomy increased CBG levels more than did prepubertal (day 25) gonadectomy, and testosterone replacement therapy reversed these effects. It is concluded that GH increases the serum CBG levels of hypophysectomized rats when it is given in a continuous manner, but not when given intermittently. The sex difference in serum CBG levels of normal rats may, therefore, be attributed to the more continuous secretory pattern of GH previously observed in female rats. Journal of Endocrinology (1989) 122, 725–732

scholarly journals Thyroid function in rats with iodine deficiency is not further impaired by concurrent, marginal zinc deficiency

1993 ◽  
Vol 70 (2) ◽  
pp. 585-592 ◽  
Author(s):  
John G. G. Smit ◽  
Daan Van Der Heide ◽  
Gerrit Van Tintelen ◽  
Anton C. Beynen
Keyword(s):  
Body Weight ◽  
Thyroid Hormone ◽  
Thyroid Function ◽  
Body Weight Gain ◽  
Male Rats ◽  
Zn Deficiency ◽  
Hormone Metabolism ◽  
Thyroid Hormone Metabolism ◽  
Marginal Zinc Deficiency ◽  
Thyroid Hormone Levels

The hypothesis tested was that Zn deficiency aggravates impaired thyroid function as induced by I deficiency. In two separate experiments male rats were fed on diets either deficient in Zn or in I, or deficient in both. An identical, restricted amount of food was given to each rat so that body-weight gain of the experimental groups was comparable. Zn deficiency was evidenced by reduced tibial Zn concentrations. I deficiency was evidenced by goitre, reduced urinary I excretion, reduced plasma thyroxine concentrations and reduced absolute amounts and concentrations of thyroxine in the thyroid. Zn deficiency had no effect on the raised thyroid weight as induced by I deficiency. Zn restriction from 184 μmol Zn/kg diet to 31 μmol Zn/kg diet, hut not to 92 μmol Zn/kg diet, significantly lowered plasma thyroxine concentration. There were no interrelated effects of Zn and I deficiencies on thyroid hormone levels. These results indicate that marginal Zn deficiency does not influence thyroid hormone metabolism in I deficiency.

scholarly journals Neuroprotection of reduced thyroid hormone with increased estrogen and progestogen in postpartum depression

2019 ◽  
Vol 39 (9) ◽  
Author(s):  
Dan Li ◽  
Yangyao Li ◽  
Yun Chen ◽  
Haiyan Li ◽  
Yuqi She ◽  
...  
Keyword(s):  
Body Weight ◽  
Thyroid Hormone ◽  
Postpartum Depression ◽  
Cell Structure ◽  
Elevated Serum ◽  
Serum Levels ◽  
Thyroid Stimulating Hormone ◽  
The Body ◽  
Free Triiodothyronine ◽  
Promising Therapeutic Approach

Abstract Background: Postpartum depression (PPD) is a common serious mental health problem. Recent studies have demonstrated that hormone therapy serves as a promising therapeutic approach in managing PPD. The present study aims at exploring the role of thyroid hormone (TH), estrogen and progestogen in patients with PPD. Methods: Initially, PPD patients were enrolled and a PPD mouse model was established. The serum levels of estradiol (E2), progesterone (P), triiodothyronine (T3), thyroxine (T4), free triiodothyronine (FT3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH) were subsequently measured. Next, in order to identify the effects of TH, estrogen and progestogen on PPD progression, mice were administrated with E2, P, contraceptives (CA), Euthyrox and methimazole (MMI). Besides, the body weight, activities, basolateral amygdala (BLA) neuron cell structure and the related gene expression of mice were analyzed. Results: The PPD patients and the mice showed elevated serum levels of T3, T4, FT3 and FT4 along with diminished E2, P and TSH levels. In the mice administered with a combination of E2, P, and MMI, decreased TH and increased estrogen and progestogen were detected, which resulted in increased body weight, normal activities, and BLA neuron cell structure. Moreover, brain-derived neurotrophic factor (BDNF) and cAMP-responsive element-binding protein (CREB) were both up-regulated in PPD mice administrated with a combination of E2, P, and MMI, which was accompanied by decreased TH and elevated estrogen and progestogen. Conclusion: Taken together, reduced TH combined with enhanced estrogen and progestogen confers neuroprotection in PPD, highlighting a potential target in prevention and treatment of PPD.

Serum free fatty acids are not involved in acute exercise-induced reduction of LPL in rat tissues

1992 ◽  
Vol 262 (4) ◽  
pp. E377-E382
Author(s):  
A. Paulin ◽  
Y. Deshaies
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Acute Exercise ◽  
Serum Levels ◽  
Male Rats ◽  
Vastus Lateralis Muscle ◽  
Adrenergic Blockade ◽  
Nonesterified Fatty Acids ◽  
Adrenergic Blocker ◽  
Exercise Induced

The present studies were designed to verify whether preventing the rise in serum levels of nonesterified fatty acids (NEFA) by adrenergic blockade would interfere with the decrease in tissue lipoprotein lipase (LPL) activity caused by acute exercise in rats. Ninety minutes before being killed, male rats were injected intraperitoneally with either saline, the beta-adrenergic blocker propranolol (25 mg/kg body weight), or the alpha 2-adrenergic blocker yohimbine (3 mg/kg body weight). Half of each group was killed at rest and the other half immediately after a 1-h run on a treadmill. LPL was determined in white adipose tissue (WAT), heart, and red vastus lateralis muscle (VLM). Exercise enhanced serum levels of NEFA 50% over resting values in saline-injected rats. The latter increase was totally abolished in animals having received propranolol or yohimbine. The activity of LPL in WAT, heart, and red VLM was approximately 35% lower in exercised rats than in resting animals. Serum triacylglycerols were also reduced by the run. Neither propranolol nor yohimbine interfered with any of these reductions. Exercise did not change serum glucose levels in saline-injected rats but decreased it in those injected with propranolol or yohimbine. Serum insulin was unchanged by exercise and by the antagonists. These findings suggest that the beta- and alpha 2-adrenergic pathways, as well as the exercise-induced rise in serum levels of NEFA, are not responsible for the early reducing effect of a 1-h run on tissue LPL activity in untrained rats.(ABSTRACT TRUNCATED AT 250 WORDS)

SERUM CONCENTRATIONS OF TESTOSTERONE, OESTROGENS, LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE IN MALE AND FEMALE RATS DURING THE CRITICAL PERIOD OF NEURAL SEXUAL DIFFERENTIATION

1979 ◽  
Vol 80 (1) ◽  
pp. 103-110 ◽  
Author(s):  
S. F. PANG ◽  
A. R. CAGGIULA ◽  
V. L. GAY ◽  
R. L. GOODMAN ◽  
C. S. F. PANG
Keyword(s):  
Post Partum ◽  
Serum Levels ◽  
Female Rats ◽  
Male Rats ◽  
Female Rat ◽  
Serum Concentrations ◽  
Male And Female ◽  
Rat Pups ◽  
Male And Female Rats ◽  
Sampling Times

Untreated male and female rat pups were killed 1–5 days post partum and the serum concentrations of testosterone, oestrogens, LH and FSH were determined by radioimmunoassay. At all five sampling times, the serum concentrations of testosterone in male rats were about three times higher than those in female rats, but serum levels of oestrogens did not differ between the sexes. Serum concentrations of LH and FSH were lower in male than in female pups. In another study, rats were decapitated 1–10 days after birth and serum concentrations of testosterone were determined with a different radioimmunoassay. Again, at all four sampling times, the concentration of testosterone was significantly higher in the male than in the female pups.

scholarly journals Nephroprotective effect of Aloe barbadensis Miller against gentamicin induced nephrotoxicity in long evans male rats

2021 ◽  
Vol 16 (1) ◽  
pp. 33-38
Author(s):  
Farjana Rahman ◽  
Mahmuda Begum ◽  
Rama Chowdhury
Keyword(s):  
Body Weight ◽  
Aloe Vera ◽  
Basal Diet ◽  
Serum Levels ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Long Evans ◽  
Nephroprotective Effect ◽  
Experimental Group

Background: Kidney is an essential excretory organ of our body. It can be damaged by poisonous effect of chemicals, toxin, prolonged and uncontrolled use of drugs. Aloe vera is a herbal plant, it can be used for the prevention and treatment of kidney damage due to its natural compatability. Objective: To observe the nephroprotective effect of Aloe vera against gentamicin induced nephrotoxicity in long evans male rats. Method: This experimental study was conducted in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st January 2018 to 30th December 2018. For this, forty five (45) apparently healthy long evans male rats, 90-120 days old, weighing between 150-200 g were taken and divided into control group (group A) and experimental group (group B - Aloe vera pretreated and gentamicin treated group). Control group was subdivided into group A1 (baseline control group) and group A2 (gentamicin treated control group). Each group consisted of fifteen (15) rats. At the beginning of the study period initial body weight of rats were measured at day 01. All the rats received basal diet for thirty (30) days. Group A1 received only basal diet for 30 consecutive days (started from day 1 to day 30). In addition to basal diet, group A2 received injection gentamicin intraperitoneally once daily in the morning (80 mg/kg/day) for last 5 days (26th to 30th) of the study period. Again, in addition to basal diet, experimental group received Aloe vera orally (200 mg/kg/day) for 30 days and injection gentamicin intraperitoneally (80 mg/kg/day) for last 5 days (26th to 30th). After measuring final body weight all the rats were sacrificed on day 31. Blood sample was collected from heart. Serum levels of creatinine and urea were estimated for assessment of kidney function. Statistical analysis was done by one way ANOVA test followed by post hoc-Bonferroni test. Result: Mean serum levels of creatinine and urea were significantly (p<0.001) higher in gentamicin treated control group in comparison to that of base line control group. Again these levels were significantly (p<0.001) lower in Aloe vera pretreated and gentamicin treated group than that of gentamicin treated control group. Conclusion: From this study, it is concluded that Aloe vera has nephroprotective effect against gentamicin induced nephrotoxicity. J Bngladesh Soc Physiol 2021;16(1): 33-38

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