scholarly journals Isolation of bacterial fish pathogen Aeromonas hydrophila and therapeutic effects of medicinal plants on its invasion

2014 ◽  
Vol 2 (1) ◽  
pp. 76
Author(s):  
Md. Tareq-Uz-Zaman ◽  
Md. Bazlur Rashid Chowdhury ◽  
Md. Rasheduzzaman Khan Dipu
Keyword(s):  
Medicinal Plants ◽  
Aeromonas Hydrophila ◽  
Control Measure ◽  
Curcuma Longa ◽  
Bacterial Pathogen ◽  
Inhibitory Effect ◽  
Infected Fish ◽  
Effective Control ◽  
Therapeutic Effects ◽  
Pathogenicity Test

Aeromonas hydrophila, a bacterial pathogen,  was isolated form Pangasius hypophthalmus. For pathogenicity test, different doses were injected intramuscularly in Barbonymus gonionotus. Crude extracts were prepared from various parts Azadirachta indica, Curcuma longa, C. zedoaria, and Callotropis gigentia and applied to B. gonionotus for 7 days. Bath treatment was done up to their tolerance level and well ventilation was confirmed for aeration and 50% water was exchanged daily. Minimum inhibitory dose was detected as 7 mg/ml. High inhibitory effect was observed in case of A. indica and mixed extract of A. indica and C. gigentia. Both A. indica and C. gigentia showed the best result with 90-95% recovery of infected fish at a dose of 7 mg/l. C. zedoaria showed moderate to weak effect with 50-60% recovery at the same dose. The present study showed that medicinal plants would be an effective control measure against A. hydrophila.

scholarly journals Evaluation of the efficacies of selected antibiotics and medicinal plants on common bacterial fish pathogens

1970 ◽  
Vol 7 (1) ◽  
pp. 163-168 ◽  
Author(s):  
T Rahman ◽  
MMR Akanda ◽  
MM Rahman ◽  
MBR Chowdhury
Keyword(s):  
Medicinal Plants ◽  
Aeromonas Hydrophila ◽  
Control Measure ◽  
Edwardsiella Tarda ◽  
Effective Control ◽  
Fish Pathogens ◽  
Different Doses ◽  
Fresh Culture ◽  
Silver Barb

An experiment was conducted to compare the efficacies of some selected antibiotics and medicinal plants against common bacterial fish pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Edwardsiella tarda. Four different antibiotics viz., CFCIN (ciprofloxacin), Renamycin (oxytetracycline), DT-10 (doxycicline) and Sulfatrim (sulphadiazine + trimethoprim) were exposed in different doses (100, 75, 50 and 25 ppm) to the culture of freshly isolated bacteria under the in vitro condition for sensitivity test and minimum inhibitory dose (MID) was determined. Based on in vitro results, antibiotics were applied to the experimental infection of Thai silver barb, Barbonymus gonionotus. CFCIN showed the best result with 100% recoveries of challenged fish in prolonged bath treatment. Medicinal plants were selected on the basis of previous studies. Crude extracts were prepared from various parts (leaves and bulb) of garlic, turmeric, akand and neem and four different doses were applied to the fresh culture of pathogenic isolates under the in-vitro condition to determine minimum inhibitory dose (MID). However, garlic offered the best result with 90.00 ± 2.89% recoveries of challenged fish in aquarium trial. Akand + neem, turmeric and akand showed moderate to weak recovery rates with the same dose. The present study thus showed that medicinal plants would be an effective control measure along with antibiotics against bacterial fish diseases. Keywords: Efficacy; Medicinal plants; Bacterial fish pathogens DOI: 10.3329/jbau.v7i1.4980 J. Bangladesh Agril. Univ. 7(1): 163-168, 2009

scholarly journals Modelling the effect of lockdown on COVID-19 pandemic in 22 countries and cities

2020 ◽  
Author(s):  
Yong Li ◽  
Yan Wu ◽  
Ximei Wang ◽  
Caihong Guo ◽  
Lianwen Wang ◽  
...  
Keyword(s):  
United States ◽  
New York ◽  
Control Measure ◽  
The United States ◽  
Inhibitory Effect ◽  
Effective Control ◽  
Cumulative Number ◽  
The United Kingdom ◽  
Number Of Patients ◽  
Short Time

Abstract Backgrounds: COVID-19 is currently spreading around the world, and the cumulative number of cases worldwide exceeded 5 million on 23 May 2020 (10:00 GMT+2). At present, many countries or cities have implemented lockdown measures. This study evaluated the inhibitory effect of lockdown measures on the pandemic by the use of lockdown or similar lockdown in 22 countries or cities. Methods: An SEIQR epidemiological model was developed to capture the transmission dynamics of COVID-19. With the data related to COVID-19 from 22 countries or cities, the optimal parameters of the model were estimated, respectively.Results: The average basic reproduction numbers of 22 countries or cities were between 1.5286-3.8067. And Russia Federation, Spain, Italy, France, Germany, the United Kingdom, Singapore, the United States of New York and the United States of New Jersey were hardest hit by COVID-19. Conclusion: Although the pandemic has not been fundamentally controlled for a short time after lockdown, lockdown was proved to be an extremely effective control measure, which significantly scaled the number of patients down, thereby reduced the harmfulness of the pandemic.

scholarly journals Use of low-cost chemotherapeutic and medicinal plants against Thai silver barb (Barbonymus gonionotus)

2013 ◽  
Vol 10 (2) ◽  
pp. 385-390 ◽  
Author(s):  
MBR Chowdhury ◽  
T Rahman
Keyword(s):  
Medicinal Plants ◽  
Field Condition ◽  
Nigella Sativa ◽  
Curcuma Longa ◽  
Low Cost ◽  
Fungal Disease ◽  
Bacterial Disease ◽  
Therapeutic Effects ◽  
Black Cumin ◽  
Highly Efficient

Studies were conducted to select and apply the effective low-cost chemotherapeutic and medicinal plants against diseased fish under laboratory and field condition. Thai silver barbs (Barbonymus gonionotus) weighing 20-25 gm were experimentally infected with a virulent bacterial pathogen (Aeromonas hydrophila) and a fungal pathogen (Aphanomyces invadans). Based on the previous studies, four chemotherapeutics, viz., salt + lime, alum, doxycycline and oxytetracycline were selected and applied to observe the therapeutic effects under laboratory and field condition. In the case of medicinal plants, leaves/ bulb / seeds/ rhizome of neem (Azadirachta indica), akand (Calotropis gigantea), garlic (Allium sativum), turmeric (Curcuma longa), shoti (Curcuma zedoaria), Indian sorrel (Oxalis corniculata), guava (Psidum guajava) and kalijira (Nigella sativa) were screened out and applied to the infected fish in laboratory and field condition to determine their efficacies. The antibiotics, both oxytetracycline and doxycycline were found to be very efficient followed by salt + lime and alum. For fungal infection, alum was excellent followed by salt + lime both under laboratory and field condition. Even in the pond trial, alum and salt + lime successfully checked the occurrence of epizootic ulcerative syndrome (EUS) and similar diseases. In the case of medicinal plants, neem and akand together reduced the occurrence bacterial and fungal disease. Garlic was very efficient to check bacterial infection but weak against fungal disease. Turmeric and arrowroot were found to be highly effective against fungal disease but moderately effective against bacterial disease. Field trial revealed that Indian sorrel and guava were highly efficient against bacterial disease but poorly efficient against fungal disease. On the other hand, arrowroot and black cumin were highly efficient against fungal disease but moderately efficient against bacterial disease. The results would be useful to the all concerned for proper health management of cultured fishes.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14932 J. Bangladesh Agril. Univ. 10(2): 385-390, 2012

Effect of the Phytochemical Agents Against the SARS-CoV and Selected Some of them for Application to COVID-19: A Mini-Review

2020 ◽  
Vol 21 ◽  
Author(s):  
Muhammad Idrees ◽  
Shamshad Khan ◽  
Nazakat Hussain Memon ◽  
Zhiyong Zhang
Keyword(s):  
Medicinal Plants ◽  
Nigella Sativa ◽  
Curcuma Longa ◽  
Basic Knowledge ◽  
Effective Control ◽  
Diverse Range ◽  
Strong Base ◽  
Vaccine Treatment ◽  
Medicinal Plant Extracts ◽  
Future Drug

Background: The aim of the present review is to provide basic knowledge about the treatment of Coronavirus via medicinal plants. Coronavirus (COVID-19, SARS-CoV, and MERS-CoV) as a viral pneumonia causative agent, infects thousands of people in China and worldwide. There is currently no specific medicine or vaccine available and it is considered a threat to develop effective novel drug or anti-coronavirus vaccine treatment. However, natural compounds to treat coronaviruses are the most alternative and complementary therapies due to their diverse range of biological and therapeutic properties. Methods: We performed an open-ended, English restricted search of Scopus database, Web of Science, and Pubmed for all available literature from Jan-March, 2020, using terms related to phytochemical compounds, medicinal plants and coronavirus. Results: The view on anti-coronavirus (anti-CoV) activity in the plant derived phytochemicals and medicinal plants give the strong base to develop a novel treatment of corona virus activity. Various phytochemicals and medicinal plant extracts have been revised and considered to be the potential anti-CoV agents for effective control and future drug development. We discuss some important plants (Scutellaria baicalensis, Psorothamnus arborescens, Glycyrrhiza radix, Glycyrrhiza uralensis , Lycoris radiate, Phyllanthus emblica, Camellia sinensis, Hyptis atrorubens Poit, Fraxinus sieboldiana, Erigeron breviscapus, Citri Reticulatae Pericarpium, Amaranthus tricolor, Phaseolus vulgaris, Rheum palmatum, Curcuma longa and Myrica cerifera) emerged to have broad spectrum antiviral activity. Conclusion: Nigella sativa has potent anti-SARS-CoV activity and it might be useful souce for developing novel antiviral therapies for coronaviruses.

scholarly journals Experimental Infection of Indigenous Climbing Perch Anabas testudineus with Aeromonas hydrophila Bacteria

2013 ◽  
Vol 22 (1-2) ◽  
pp. 105-114 ◽  
Author(s):  
M Farhad Hossain ◽  
M Mamnur Rahman ◽  
MA Sayed
Keyword(s):  
Body Weight ◽  
Water Temperature ◽  
Aeromonas Hydrophila ◽  
Bacterial Load ◽  
Infected Fish ◽  
Pathogenicity Test ◽  
Anabas Testudineus ◽  
Climbing Perch ◽  
Average Body ◽  
Average Body Weight

The present study was conducted to know the pathogenicity and LD50 of Aeromonas hydrophila isolated from diseased climbing perch Anabas testudineus against apparently healthy homologous fish and the distribution of the bacteria in the organs of the experimentally infected fish. A total of 10 fish of average body weight of 18 g were used. For pathogenicity test, two different doses viz. 9.2 × 107 and 9.2 × 106 CFU/fish were injected intramuscularly. Pathogenicity of A. hydrophila was confirmed at water temperature of 28.53oC by the mortality of 40% to 100% of all tested fish within 4 to 9 days. The highest bacterial load was found to be 9.4 × 108 CFU/g in the intestine and the lowest bacterial load was found to be 2.8 × 103 CFU/g in the kidney of the tested fish. Four different serial concentrations, vide 9.2 × 107, 9.2 × 106, 9.2 × 105 and 9.2 × 104 CFU/fish of the bacteria were injected in each of four different groups of 10 fish. The calculated LD50 value at 27.3oC water temperature was 2 × 107 CFU/fish of 18 g of average body weight. In all the cases of intramuscular injection, external pathology was found. Reddish anal region and fin bases were observed. Injected A. hydrophila was re-isolated from liver, kidney and intestine of the challenged fish. It was understood that the isolate was a high virulent pathogen for A. testudineus.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16472 Progress. Agric. 22(1 & 2): 105-114, 2011

scholarly journals Medicinal plants of the Unified Health System (Sistema Único de Saúde) with antifungal potential

2021 ◽  
pp. 1-9
Author(s):  
Mônica Jachetti Maciel ◽  
Claudete Rempel ◽  
Amanda Luisa Stroher ◽  
Patrícia Caye Bergmann ◽  
Diorge Jônatas Marmitt
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Health System ◽  
Herbal Medicines ◽  
Inhibitory Effect ◽  
Fungal Species ◽  
Therapeutic Effects ◽  
Alternative Source ◽  
Unified Health System ◽  
Antifungal Action

Medicinal plants synthesize various secondary metabolites that can be used for therapeutic and antimicrobial purposes. In Brazil, the Unified Health System (SUS) offers several herbal medicines as an alternative in the treatment of various diseases. Considering the importance of these plants in the production of chemicals that expand therapeutic options and improve the health of SUS users, this review was carried out to quantitatively determine the antifungal activity of plants used as phytotherapeutics at RENAME. The selection of papers was performed at three distinct stages: examining and choosing titles related to antifungal action, reading the abstracts, and reading the whole selected articles. This review selected 22 studies of interest; 12 of them were conducted in Brazil and 10 were carried out in other countries. The papers chosen tested the growth inhibitory effect of plants against fungal species of agricultural and health importance, ranging from filamentous to yeast-like fungi, and Candida albicans was the most tested species. The growth of 39 fungal species were inhibited by some concentration of the extract used, with either an increase or decrease in antifungal activity depending on the extract used. The most frequently analyzed plant was the species Schinus terebinthifolius Raddi., studied in seven papers. The results found demonstrate the importance of analyzing medicinal plants and incorporating plant-based medicines in healthcare as an alternative source of treatment, highlighting the need for studies that evaluate the mechanisms action of their cytotoxicity and therapeutic effects in the human body.

scholarly journals Evaluation of Medicinal Plants Through Fish Feed Against Bacterial Fish Disease

2013 ◽  
Vol 19 (2) ◽  
pp. 151-159 ◽  
Author(s):  
M Muniruzzaman ◽  
MBR Chowdhury
Keyword(s):  
Medicinal Plants ◽  
Aeromonas Hydrophila ◽  
Allium Sativum ◽  
Labeo Rohita ◽  
Bacterial Pathogens ◽  
Infected Fish ◽  
Edwardsiella Tarda ◽  
Fish Feed ◽  
Fish Disease ◽  
Silver Barb

Experiments were conducted maintaining under aquarium and pond conditions using feed containing medicinal plants/extracts was evaluated on artificially and naturally infected fish with bacterial pathogens. When the fish were exposed to high bacterial pathogens Aeromonas hydrophila Ah-11, Pseudomonas fluorescens Pf-13 and Edwardsiella tarda Et-70, recovery of the infected fish varied with the pathogens challenged and feed treatments. Fish feed containing bulb extract of Allium sativum (3% feeding) showed significantly (p<0.01) high therapeutic effect recovering the infected fish (Thai silver barb, Barbodes gonionotus) with A. hydrophila (100 ± 0% recovery) and P. fluorescens (90±0% recovery). A similar result was observed in the case of pangas fish feed containing decoction of leaves of Calotropis gigantea where 100 ± 0% E. tarda infected Thai pangas (Pangasius hypophthalmus) were found to be cured. These two types of herbal feed offered similar result when applied to the naturally ulcer-affected Thai silver barb and Thai pangas fingerling under aquarium condition. In pond condition, the herbal feed containing bulb extract of A. sativum was applied experimentally to A. hydrophila infected different fish species maintained in different individual ponds where, 91.67 ± 2.35% Thai silver barb, 84.99 ± 2.35% Rui (Labeo rohita) and 74.99 ± 2.35% Mrigal (Cirrhinus cirrhosus) were found to be recovered. In contrast, plants mixed pangas feed containing decoction of leaves of C. gigantea was cured 94.44 ± 3.84% E. tarda infected Thai pangas. The other medicinal plants used in this study showed medium to weak effect recovering the bacterial infected fish.DOI: http://dx.doi.org/10.3329/pa.v19i2.16956 Progress. Agric. 19(2): 151 - 159, 2008

scholarly journals The Effectiveness of Turmeric Enriched Pellets in Curing the Gill of Clarias batrachus Infected with Aeromonas hydrophila

2021 ◽  
Vol 934 (1) ◽  
pp. 012006
Author(s):  
M Riauwaty ◽  
Windarti
Keyword(s):  
Aeromonas Hydrophila ◽  
Curcuma Longa ◽  
Histological Study ◽  
Clinical Signs ◽  
Positive Control ◽  
Negative Control ◽  
Infected Fish ◽  
Clarias Batrachus ◽  
Control Fish ◽  
Gill Structure

Abstract Curcumin is an antibiotic agent extracted from Curcuma longa. To understand the effectiveness of curcumin in curing the gill of Clarias batrachus infected with Aeromonas hydrophila, a study was conducted from August to November 2020. There were 3 treatments applied, namely turmeric enriched pellets 0.7g/kg (P1); 0.9g/kg (P2) and 1.1g/kg (P3). Prior to the treatment, the fish was infected with A. hydrophila (0.1 m of A. hydrophila culture, 1.0x108cells/ml). The infected fish was kept for 6 days until the clinical signs of MAS disease occurred. By the 7th day, the fish was feed with turmeric enriched pellets. For negative control (Cn) the fish was not infected with A. hydrophila nor treated with turmeric, while the positive control (Cp) was infected, but no turmeric treatment. The fish was reared for 30 days and by the end of the experiment, the gill was removed and processed for histological study (5L sliced and HE stained). Result indicated that the gill of the negative control fish was normal, while that of the positive control shown abnormalities such as hemorrhages, necrotic cells, fused lamellae and enlarged cartilaginous cells. The turmeric treated fish shown less damage in the gill. The best results were obtained in C3, as the gill structure was almost normal with slightly hemorrhage. In the C1 and C2 treated fishes, the gill shown light abnormality such as fused lamella and hemorrhage. Data obtained shown that the consumption of turmeric enriched pellet was effective to cure the A. hydrophyla infected gill.

Control of Bulking in an Extended Aeration Sewage Treatment Plant

1972 ◽  
Vol 7 (1) ◽  
pp. 13-20
Author(s):  
D.D.P. Cane ◽  
G.J. Farguhar
Keyword(s):  
Dissolved Oxygen ◽  
Control Measure ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Effective Control ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Mixed Liquor ◽  
Air Supply

Abstract A case of filamentous activated sludge bulking was investigated at an extended aeration plant treating wastes from a highway service centre. The purpose of the study was to find an effective control measure for the bulking and, if possible, to determine the cause of the bulking condition. Experiments were conducted to determine the effects upon bulking of: (a) a controlled copper dosage to the mixed liquor, (b) variations in the organic loading rate, and (c) maintenance of high and low dissolved oxygen concentrations in the mixed liquor. The microorganism responsible for the bulking condition was tentatively identified as Sphaerotilus natans. Extensive growths of these bacteria occurred in the sludge when the mixed liquor dissolved oxygen concentration dropped below 0.5 mg/1. Such dissolved oxygen levels had frequently occurred at this plant due to the use of timed aeration cycles and the use of insufficient air supply during peak loading periods. Variations in organic loading rates were found to have no adverse effect on sludge settleability. When bulking was slight, the condition could be improved by increasing the dissolved oxygen level to 1.5 mg/1, or more. The rate at which the filamentous growths could be eliminated from a highly filamentous sludge by increased aeration was very slow, but could be greatly increased by the simultaneous feeding of copper to the mixed liquor at a dosage rate of 1.0 mg/1, based on the raw sewage flows.

Traditional aspects to sciatic pain and allied therapies from Persian medical reports

2020 ◽  
Vol 17 ◽  
Author(s):  
Ramin Ansari ◽  
Amirhossein Dadbakhsh ◽  
Fatemeh Hasani ◽  
Fatemeh Hosseinzadeh ◽  
Zohreh Abolhassanzadeh ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Zingiber Officinale ◽  
Therapeutic Effects ◽  
School Of Medicine ◽  
Sciatic Pain ◽  
Novel Drugs ◽  
Zingiber Officinale Roscoe ◽  
Medical Reports ◽  
Traditional Persian Medicine ◽  
Increasing Demand

: Sciatica is a common back pain caused by irritation of the sciatic nerve. Current pharmacologic therapies have proven inadequate for many patients with sciatic pain. On the other hand, there is a global increasing demand toward the use and administration of natural medicaments for this disorder. Traditional Persian Medicine (TPM) is a school of medicine and a medicinal plant based resource for clinical studies put forward by Persian scholars. The aim of the present study is to gather and study the effectiveness of all medicinal plants from five main Persian pharmacopeias. Furthermore, different data bases such as PubMed and Scopus have been checked to derive relevant activities for these plants. In all, 99 medicinal plants related to 42 families have been authenticated. Asteraceae and Apiaceae were the most frequent families and Roots and Seeds were the most reported botanical parts. The employed routs of administration were oral (54%), topical (33%) and rectal (13%). Anti-inflammatory, analgesic activity and anti-nociceptive properties of medicines are known as some main mechanisms to manage sciatic pain. These functions are possessed by 30%, 15%, and 15% of the studied plants, respectively. Medicaments that can be introduced as lead agents for further investigation are Zingiber officinale Roscoe, Olea europaea L., Strychnos nux-vomica L and Artemisia vulgaris L which showed all of these properties in previous studies. Hence, conducting adducible clinical trials using these lead agents may lead to novel drugs with lesser undesirable and much more therapeutic effects on controlling sciatic pain.

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