scholarly journals Occurrence of pathotypes of Diplocarpon rosae on roses in Belgium

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 536-538 ◽  
Author(s):  
L. Leus ◽  
G. Kegelaers ◽  
E. Van Bockstaele ◽  
M. Höfte
Keyword(s):  
Black Spot ◽  
Natural Resistance ◽  
Artificial Infection ◽  
Diplocarpon Rosae ◽  
In Vitro Plantlets ◽  
Screening Protocol ◽  
Different Levels

Monospore cultures of black spot (Diplocarpon rosae Wolf) were established from isolates collected on outdoor grown roses at different locations in Belgium. These monospore cultures were tested on rose cultivars and species with different levels of natural resistance to examine the possible existence of different pathotypes. For this purpose a screening protocol including scoring after artificial infection was developed on in vitro plantlets. Differences between the examined isolates proved that there exist different pathotypes of Diplocarpon rosae in Belgium.

scholarly journals Amphidiploid Induction from Diploid Rose Interspecific Hybrids

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 292-295 ◽  
Author(s):  
Yan Ma ◽  
David H. Byrne ◽  
Jing Chen
Keyword(s):  
Interspecific Hybrids ◽  
Diploid Species ◽  
Shoot Culture ◽  
Black Spot ◽  
Colchicine Treatment ◽  
Chromosome Counts ◽  
Diplocarpon Rosae ◽  
Breeding Research ◽  
Colchicine Solution

A high priority in rose (Rosa spp.) breeding research is the transfer of disease resistance, especially to black spot (Diplocarpon rosae Lib.), from wild diploid Rosa species to modern rose cultivars. To this end, amphidiploids (2n = 4x = 28) were induced with colchicine from five interspecific diploid (2n = 2x = 14) hybrids involving the black spot resistant diploid species R. wichuraiana Crép, R. roxburghii Thratt., R. banksiae Ait., R. rugosa rubra Hort., and R. setigera Michaux. Two application procedures (agitation of excised nodes in colchicine solution or tissue culture of shoots on medium with colchicine), five colchicine concentrations (0.0, 1.25, 2.50, 3.76, and 5.01 mmol), and five durations (2, 3, 5, 8, and 10 d) were used. After colchicine treatment, the materials were cultured in vitro and the surviving explants were examined for the “gigas” characteristics typical of doubled diploids. Chromosome counts of morphologically suspect genotypes confirmed 15 amphidiploids among 1109 plants that survived colchicine treatment. Although the effect of colchicine treatment varied some among interspecific hybrids, 2.50 mmol for 48 h of node agitation or 1.25 mmol for at least 5 d of shoot culture were optimal.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

Discovery of New AKT1 Inhibitors by Combination of In silico Structure Based Virtual Screening Approaches and Biological Evaluations

2019 ◽  
Author(s):  
Filip Fratev ◽  
Denisse A. Gutierrez ◽  
Renato J. Aguilera ◽  
suman sirimulla
Keyword(s):  
Virtual Screening ◽  
Success Rate ◽  
Protein Interactions ◽  
In Silico ◽  
Biological Data ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Screening Protocol ◽  
Screening Approaches

AKT1 is emerging as a useful target for treating cancer. Herein, we discovered a new set of ligands that inhibit the AKT1, as shown by in vitro binding and cell line studies, using a newly designed virtual screening protocol that combines structure-based pharmacophore and docking screens. Taking together with the biological data, the combination of structure based pharamcophore and docking methods demonstrated reasonable success rate in identifying new inhibitors (60-70%) proving the success of aforementioned approach. A detail analysis of the ligand-protein interactions was performed explaining observed activities.<br>

Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets

2008 ◽  
Vol 96 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Xiaojiao Han ◽  
Hongqiang Yang ◽  
Kaixuan Duan ◽  
Xinrong Zhang ◽  
Haizhou Zhao ◽  
...  
Keyword(s):  
Sodium Nitroprusside ◽  
Malus Hupehensis ◽  
In Vitro Plantlets

scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

2021 ◽  
pp. 088532822110038
Author(s):  
Mohammad Yousef Memar ◽  
Mina Yekani ◽  
Hadi Ghanbari ◽  
Edris Nabizadeh ◽  
Sepideh Zununi Vahed ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Carbapenem Resistant ◽  
Toxicity In Vitro ◽  
Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

In Vitro Zinc-Air Battery Evaluation for Use in Intraoral Medical Devices

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Miguel Amaral ◽  
Francisco do Vale ◽  
João Silva ◽  
Francisco Caramelo ◽  
Germano Veiga
Keyword(s):  
Medical Devices ◽  
Lithium Batteries ◽  
Artificial Saliva ◽  
Robot Arm ◽  
Climatic Chamber ◽  
Electrical Capacity ◽  
Air Battery ◽  
Different Levels ◽  
Zinc Air Batteries

The aim of the present work was to evaluate the possibility of using zinc-air batteries in intraoral medical devices. We analyzed the electrical behavior of zinc-air batteries when submitted to different levels of temperature, humidity, and limited quantities of air. The experimental setup was divided in three different parts. Firstly, a set of batteries were tested within a climatic chamber and subjected to discharging tests similar to those recommended by the manufacturer. The climatic chamber allowed an accurate variation of humidity and temperature. Secondly, the batteries were placed in a small prototype of intraoral medical device and tested in the absence of air. Lastly, we used a robot arm to repeatedly immerse the prototype in artificial saliva. The results obtained demonstrated the viability of zinc-air batteries as a power solution for intraoral medical devices, as they tolerate high levels of humidity and are capable of working with limited quantities of air. In addition, this kind of battery presents a volume to electrical capacity ratio more than three times higher than lithium batteries, which may open important improvement for powered medical devices.

Sign in / Sign up

Export Citation Format

Share Document