scholarly journals Effect of carbohydrates in grass silage-based diets on in sacco ruminal degradability of barley (Hordeum vulgare L. cv. Lomerit) grain ground to different particle sizes

2009 ◽  
Vol 54 (No. 6) ◽  
pp. 260-269
Author(s):  
B. Niwińska
Keyword(s):  
Particle Size ◽  
Hordeum Vulgare ◽  
Latin Square ◽  
Barley Grain ◽  
Particle Sizes ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Degradation Rates ◽  
Size Interaction ◽  
In Sacco

The effect of carbohydrates included in grass silage-based diets on <I>in sacco</I> degradability of dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and cellulose (CE) of barley (<I>Hordeum vulgare</I> L. cv. Lomerit) grain ground to different particle sizes was studied using 3 ruminally cannulated cows. The measurements were carried out as a 3 × 2 Latin square design with treatments being carbohydrates in the concentrate portion of diets (starch in diet I vs. pectin in diet II vs. sucrose in diet III) and particle size of ground barley grain (1.0 vs. 5.7 mm). The diet synchronization index between N and energy supply during the day was an average of 0.8. The rumen degradability of barley grain compounds was influenced by the type of carbohydrates included in grass silage-based diets. Higher rumen degradation rates (<I>P</I> < 0.05) of barley CP, NDF and CE after 8 h of incubation and of CP and CE after 16 h of incubation were found in the rumen of cows receiving the diet containing sucrose compared with degradation rates found in the rumen of cows receiving the diet containing pectin or starch. The effective degradability of DM, CP, NDF and CE of barley grain in the rumen of cows receiving diets I, II and III was similar (77, 74, 39 and 41%, respectively). Replacing barley starch or beet pulp pectin with molasses sucrose in the grass silage-based diet resulted in the higher rate of barley CP, NDF and CE degradation after 8 h of incubation and of CP and CE after 16 h of incubation. It seems that the energy availability from carbohydrates affected the ruminal microbial activity as a consequence of the degradation rate of substrates. Higher (<I>P</I> < 0.01) rumen degradability of CP, NDF and CE after 2, 4, 8, 16 and 24 h of incubation was estimated for 1.0 mm particle size in comparison with that found for grain ground to 5.7 mm particle size, and the degradability of CP after 48 h of incubation was not different (<I>P</I> > 0.05). The degradability of barley grain CP, NDF and CE during incubation time was not influenced by the diet composition by particle size interaction (<I>P</I> > 0.05). The increase in barley grain particle size from 1.0 to 5.7 mm slowed down the rumen digestion of barley DM, CP, NDF and CE, probably due to restricted access to microbial digestion.

scholarly journals Effect of the type of non-fibre carbohydrates in grass silage-based diets on in sacco ruminal degradability and protein value of lupin (Lupinus angustifolius L. cv. Sonet) seeds ground to different particle sizes

2011 ◽  
Vol 56 (No. 5) ◽  
pp. 231-241 ◽  
Author(s):  
B. Niwińska ◽  
M. Andrzejewski
Keyword(s):  
Particle Size ◽  
Small Intestine ◽  
Lupinus Angustifolius ◽  
Seed Particle ◽  
Particle Sizes ◽  
Experimental Conditions ◽  
Grass Silage ◽  
Lupin Seed ◽  
Lupin Seeds ◽  
In Sacco

The study was conducted to find out the effects of the type of non-fibre carbohydrates (NFC) included in grass silage-based diets on in sacco degradability of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF) of lupin (Lupinus angustifolius L. cv. Sonet) seeds ground to different particle sizes. Measurements of degradability were carried out as a 3 &times; 2 &times; 3 &times; 3 experimental design of treatments with carbohydrates included in diets as primary sources of NFC (starch vs. pectin vs. sugars), particle size of ground seeds (1.0 vs. 5.7 mm), period of the trial (1 vs. 2 vs. 3) and cannulated cow (1 vs. 2 vs. 3). The variables of in sacco kinetics were calculated according to the equations of &Oslash;rskov and McDonald (1979) for degradation data corrected for the particle loss. The protein value of lupin seeds for ruminants was expressed as the amount of protein digested in the small intestine, and it was estimated based on the obtained characteristics of degradability. Under our experimental conditions, the type of NFC included in the grass silage-based diets and the particle size of seeds affected the course and extent of rumen degradation of lupin seed nutrients. Higher values of DM and CP degradation were estimated after 8 and 16 h of incubation (P &lt; 0.05). The higher rate of degradation of insoluble but potentially degradable fractions (P &lt; 0.05) and higher effective degradability (P &lt; 0.05) were found out in the rumen of cows receiving diets containing sucrose compared with cows receiving diets containing starch. In comparison with starch and pectin, sucrose decreased the amount of protein digested in the small intestine (P &lt; 0.05). The results suggested that the type of NFC determined ruminal microbial activity. An enlargement of the lupin seed particle size from 1.0 to 5.7 mm decreased the rumen degradability of lupin seed nutrients (P &lt; 0.05) and increased the amount of protein digested in the small intestine (P &lt; 0.05). The results indicated that feeding standards should consider the influence of the grinding level of lupin seeds in order to assess their nutritional value.

scholarly journals Feeding strategies for improving productivity of growing steers fed grass silage

2008 ◽  
Vol 88 (4) ◽  
pp. 685-692
Author(s):  
D. R. Ouellet ◽  
L. Faucitano ◽  
D. Pellerin ◽  
M. D’Amours ◽  
R. Berthiaume
Keyword(s):  
Particle Size ◽  
Soybean Meal ◽  
Nitrogen Balance ◽  
Average Daily Gain ◽  
Latin Square ◽  
Feeding Strategies ◽  
Beef Steers ◽  
Plasma Urea ◽  
Grass Silage ◽  
Ground Corn

Two experiments were conducted to determine the relationship between corn particle size and soybean meal treatment on growth, diet digestibility, and nitrogen balance of growing steers. In the first experiment, 40 medium-frame beef steers (250 ± 11 kg) were fed individually for 140 d a diet based on grass silage offered for ad libitum consumption and supplemented with either 3.5 kg of DM d-1 of cracked corn (CC) or ground corn (GC) and with 450 g of DM d-1 of solvent extracted (SS) or lignosulfonate-treated soybean meal (Soypass™ SP). Dry matter intake was not affected by treatments and averaged 8.6 ± 0.3 kg d-1 (P > 0.10). Average daily gain was higher for animals receiving the ground corn than those fed cracked corn. Feed to gain ratio was not affected by treatments. There was an interaction between treatments for plasma urea-N concentration, with source of soybean meal having no effect with CC. When compared with SS, SP supplement reduced plasma urea-N when fed with GC. No effect of soybean meal and its interaction with corn processing was observed on growth performance. In the second experiment four additional steers were used in a 4 × 4 Latin square design to evaluate diet digestibility and nitrogen balance. There was an interaction between treatments for starch apparent digestibility and N retained, the values being respectively, 90, 86, 93, and 92% (SEM = 1.2; P < 0.01) and 36, 42, 44 and 41 g d-1 (SEM = 2.8; P < 0.03) for CCSS, CCSP, GCSS and GCSP, respectively. Altogether, the results indicate a slight advantage to reduce particle size of corn in growing steers fed grass silage. However, soybean meal treatment resulted in limited effects on growth and digestion. Key words: Rumen carbohydrate, undegradable protein, performance, steers

Effects of lucerne hay and barley grain particle sizes on chewing activity, rumen pH and milk composition of Holstein dairy cows

2007 ◽  
Vol 2007 ◽  
pp. 130-130
Author(s):  
A.R. Bayat ◽  
R. Valizadeh ◽  
A.A. Nasserian
Keyword(s):  
Particle Size ◽  
Dairy Cows ◽  
Milk Fat ◽  
Milk Composition ◽  
Barley Grain ◽  
Particle Sizes ◽  
Milk Fat Depression ◽  
Rumen Ph ◽  
Maize Grain ◽  
Chewing Activity

NRC (2001) recommended 250 g/kg dietary neutral detergent fibre (NDF) with a minimum of 190 g/kg dietary forage NDF to prevent milk fat depression. This recommendation does not account for the differences in forage particle size. The forage particle size requirements of dairy cows fed barley grain-based diets can differ from those fed maize grain-based diets (Yang and Beauchemin, 2006). The objective of this experiment was to evaluate the effects of lucerne hay and barley grain particle sizes on chewing activity, rumen pH, milk yield and milk composition using barley grain-based diets.

scholarly journals Rumen fill and digesta kinetics in lactating Friesian cows given two levels of concentrates with two types of grass silage ad lib

1991 ◽  
Vol 66 (3) ◽  
pp. 381-398 ◽  
Author(s):  
J. Gasa ◽  
K. Holtenius ◽  
J. D. Sutton ◽  
M. S. Dhanoa ◽  
D. J. Napper
Keyword(s):  
Particle Size ◽  
Rate Constant ◽  
Rumen Fluid ◽  
Latin Square ◽  
Grass Silage ◽  
Fractional Rate ◽  
Fibre Degradation ◽  
Kinetics Of ◽  
Resting Period ◽  
Friesian Cows

Four lactating Friesian cows with permanent cannulas in the rumen and proximal duodenum were given early (EC)- or late (LC)-cut grass silage ad lib., each with either 3 or 9 kg concentrate dry matter (DM)/d in a 4 x 4 Latin square design starting about 10 weeks after calving. Feed was offered twice daily at 08.30 hours and 15.30 hours. Periods lasted 5 weeks and measurements were made in the last 15 d. The higher amount of concentrates increased total DM intake but reduced silage DM intake and the fractional rate of degradation of silage-fibre DM. Later cutting date of silage had no effect on DM intake or the fractional rate of degradation of silage-fibre DM but reduced potential degradability of silage fibre. Dilution rate of CoEDTA in rumen fluid was greater during the day (eating period) than during the night (resting period). Dilution rates measured at the duodenum were lower than those measured in the rumen, but neither was affected by diet. Silage-particle passage rates were measured by use of ytterbium-labelled silage fibre (Yb-fibre) and chromium-mordanted faecal particles (Cr-faeces) and samples were taken at the duodenum and in the faeces. Values for slower rate constant (k1) and transit time were higher and for faster rate constant (k2) were lower for Yb-fibre than for Cr-faeces, but differences in total mean retention time were inconsistent. Values for k1 for both markers and k2 for Yb-fibre only were similar at both sampling sites, but values for k2 for Cr-faeces were lower in the faeces. No diet effects were established with Yb-fibre but, with Cr-faeces, k1 was reduced by more concentrates and EC-silage. Daily mean weights of wet digesta, liquid, neutral-detergent fibre (NDF) and indigestible NDF in the rumen were greater with LC-silage but were unaffected by the amount of concentrates whereas weight of rumen DM was increased by the amount of concentrates only. Maximum rumen fill occurred at 18.00 hours with all diets. Particle-size distribution of rumen contents did not vary markedly during the day. Mean particle size was generally greater with LC-silage than EC-silage. Very small particles, passing through the 0.3 mm screen, constituted about half the rumen DM. It is concluded that rumen fill could have limited intake of LC-silage but not EC-silage. The reduced silage intake with greater concentrate intake was associated with a reduction in fibre degradation rate and an increase in rumen DM fill but no other consistent effects on weight or kinetics of rumen fractions were established.

Effect of supplementary energy and protein feeding on the true digestion of grass-silage organic matter, cell walls and nitrogen estimated by the combined synthetic-fibre-bag method in cows

1992 ◽  
Vol 72 (3) ◽  
pp. 671-678 ◽  
Author(s):  
Tuomo Varvikko ◽  
Aila Vanhatalo
Keyword(s):  
Organic Matter ◽  
Cell Walls ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Concentrate Supplementation ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Intestinal Digestion ◽  
Ruminal Degradation ◽  
The Individual

Four ruminally and duodenally cannulated non-lactating Finnish Ayrshire cows were used in a balanced 4 × 4 Latin square to study the effect of different concentrate supplements on the true partial and total-tract digestion (TTD) of grass silage, estimated by using the combined rumen-bag-intestinal-bag method. The cows were fed, at maintenance level, grass silage alone or supplemented with good-quality ground barley, ground barley and rapeseed meal, or ground barley and soybean meal. The determination of the proportion of grass silage degraded in the rumen (RD) was based on disappearance of feeds from nylon bags during the rumen incubation as a function of time, using the outflow rate of k = 0.0625. The intestinal digestion (ID) was estimated by the mobile-bag method with the residues that resisted degradation during the 16-h rumen incubation. Combination of these two was calculated to provide the TTD. Concentrate supplementation always caused a clear and consistent decline in rumen degradation and TTD of organic matter (OM), neutral detergent fibre (NDF) and Kjeldahl-N of grass silage but had no real influence on its ID. The type of concentrate, however, had only little effect. The average TTD of NDF was 16% lower than that of OM, but TTD of N was always very much higher than the respective value for OM. The results indicate that concentrate supplementation decreases the total-tract digestion of OM, cell walls and nitrogen of grass silage owing to impaired ruminal degradation. The combined bag method appears a convenient tool to provide digestion coefficients close to the true feed digestion of the individual feeds. Key words: Grass silage, nylon bag, mobile bag, combined bag, ruminal degradation, intestinal digestion, true digestion

EFFECTS OF CHANGES IN ATTRIBUTES OF RETICULAR CONTRACTION ON FECAL PARTICLE SIZES IN CATTLE

1990 ◽  
Vol 70 (1) ◽  
pp. 159-166 ◽  
Author(s):  
E. K. OKINE ◽  
G. W. MATHISON ◽  
R. T. HARDIN
Keyword(s):  
Particle Size ◽  
High Weight ◽  
Geometric Mean ◽  
Latin Square ◽  
Design Experiment ◽  
Particle Sizes ◽  
Low Weight ◽  
Large Particles ◽  
Mean Size ◽  
Period Duration

A 3 × 3 Latin square design experiment was conducted with three ruminally-cannulated Hereford steers (605 ± SD 98 kg) to determine the effects of changes in reticular contraction characteristics on the distribution of fecal particle size of cattle fed at maintenance. Addition of 0 (control; C) 9 (low weight; LW) or 18 (high weight; HW) kg weight to ventral sac of the rumen of steers linearly increased (P = 0.03) the geometric mean size of fecal particles. During the 1.5-h feeding period, duration and amplitude of reticular contractions linearly increased (P = 0.04) and decreased (P = 0.08), respectively, with the addition of weights. Frequency of reticular contractions increased linearly (P = 0.05) and quadratically (P = 0.07), reaching a maximum of 1.7 min−1 in LW steers, 1.6 min−1 and 1.4 min−1 in HW and C steers, respectively. Duration of reticular contractions, but not amplitude or frequency, was linearly related to the proportion of large particles and to the geometric mean size of fecal particles (r = 0.90, P = 0.002; and r = 0.61, P = 0.08, respectively). It was concluded that the increase in fecal geometric mean and the change in the distribution of fecal particle sizes with increasing weights in the rumen was associated with increases in the duration of reticular contractions rather than changes in frequency or amplitude. Key words: Steers, reticular contractions, fecal particle size, rumen, weight

scholarly journals Autumn-cut grass silage as roughage component in dairy cow rations. 2. Rumen degradation, fermentation and kinetics

1993 ◽  
Vol 41 (3) ◽  
pp. 221-234
Author(s):  
H. De Visser ◽  
H. Huisert ◽  
A. Klop ◽  
R.S. Ketelaar
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
High Moisture ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Branched Chain ◽  
Gluten Feed

In a 4 x 4 Latin square experiment the effects of DM content and/or the extent of fermentation in grass silages on the pattern of rumen fermentation and rumen kinetics were studied. In a separate study two cows were used to measure the rate of degradation using the dacron bag technique. Four rumen cannulated dairy cows were used to measure rumen fermentation pattern, rumen kinetics were measured in three of these animals. Basal diets (70% of total DM) consisted of maize silage, moist ensiled beet pulp, moist ensiled maize gluten feed, moist ensiled brewers' grains and a concentrate mixture. The remainder of the diet (30% of total DM) was wilted grass silage (WGS), high moisture grass silage with molasses (MGS), high moisture silage with formic acid (FGS) or wilted grass silage with additional water (WW). All diets were fed as complete feeds. pH of the rumen fluid was lower on the MGS and FGS diets. Concentrations of total VFA, acetic acid, ammonia and branched-chain fatty acids (BCFA) were highest on high moisture diets (MGS and FGS). Rates of clearance and digestion of the organic matter (OM) fractions were or showed tendencies towards being negatively influenced by both MGS and FGS, but remained unaffected by WGS and WW. Degradability of the grass silages was influenced by fermentation in the silo (lower digestible fractions and higher soluble fractions), as were rates of degradation (higher). Results of the degradability measured on the basal diet ingredients were in agreement with published literature and showed a strong correlation between OM digestibility in vitro and the undigestible fraction.

Effect of particle size on in situ ruminal disappearances of pea (Pisumsativum) organic matter, proteins and starch in dairy cows

2000 ◽  
Vol 80 (1) ◽  
pp. 203-206 ◽  
Author(s):  
C. Bayourthe ◽  
R. Moncoulon ◽  
F. Enjalbert
Keyword(s):  
Particle Size ◽  
Organic Matter ◽  
Dairy Cows ◽  
Crude Protein ◽  
Rumen Degradation ◽  
Pea Flour ◽  
Effect Of Particle Size ◽  
Pea Seeds ◽  
In Sacco

Ruminal disappearance characteristics of OM, CP and starch relative to particle size of dehulled pea seeds were determined using the in sacco method. Fine grinding (≤502 µm) highly increased the rapidly CP and starch degradable fractions and the rate of degradation of these nutrients. Key words: Pea flour, particle size, rumen degradation, crude protein, starch

The effect of drying method on the degradation characteristics of grass silage

1993 ◽  
Vol 1993 ◽  
pp. 168-168
Author(s):  
A T Chamberlain ◽  
F Endalew
Keyword(s):  
Linear Regression ◽  
Dry Matter ◽  
Latin Square ◽  
Microwave Oven ◽  
Protein Damage ◽  
Drying Method ◽  
Grass Silage ◽  
Freeze Dried ◽  
Drying Techniques ◽  
In Sacco

The metabolisable protein system (AFRC, 1992) requires the measurement of the dynamics of the degradation of protein in the rumen. However many drying techniques used during sample preparation cause protein damage and denaturation which may affect the subsequent assessment. The objective of this work was to compare the dry matter and nitrogen degradation profiles of samples of grass silage dried using a range of techniques.One kilogram samples of first cut grass (Lolium perenne) silage (156 g CP/kg DM) were air dried (AD), freeze dried (FD), dried at 50 (OD50) or 100°C (OD100) in a still air oven or dried in a microwave oven (MD) on the ‘defrost’ setting. In sacco degradability was assessed in fistulated yearling wether sheep, using a 5x5 Latin square and incubation times of 3,6,12,24 and 48 hours. Bags size was nn x nn cm with a sample size of 3 g. Bags were rinsed and frozen upon removal, mechanically washed and dried at 60°C before analysis. Dry matter and nitrogen disappearance data for each sheep was fitted to the model y = a + b(1-e-ct) using non-linear regression.

Using fistulated sheep to compare in sacco and in vitro rumen degradation of selected feeds

2011 ◽  
Vol 51 (11) ◽  
pp. 1015 ◽  
Author(s):  
Abdul Shakoor Chaudhry ◽  
Ruba A. I. Mohamed
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Water Soluble ◽  
Prediction Ability ◽  
In Vitro Method ◽  
Rumen Degradation ◽  
Degradation Data ◽  
Almost All ◽  
In Sacco

Despite its beneficial role in almost all feed evaluation systems, the in sacco method is inconsistent and labour intensive in estimating the degradation of ruminant feeds. This study compared a rumen fluid-based in vitro method with the in sacco method to estimate degradation of 12 feeds comprising six concentrates and six grasses representing three fields during two seasons of winter and spring. Three feed groups (n = 4) were formed by using two grasses (one per season) and two concentrates. These feeds were incubated alongside blanks, in duplicate, for various hours either in sacco in three fistulated sheep or in vitro by using rumen fluid from the same sheep over three periods using two separate 3 × 3 Latin square designs. The in sacco and in vitro data were statistically compared for the effects of sheep, feed group and period on degradation at each incubation time within each method. The degradation data were fitted exponentially to derive water soluble (a), insoluble (b) and degradation rate (c) constants to estimate effective degradability at rumen outflow rate of 0.02 (P0.02) for each feed in each method. These values were statistically compared for variations within and between feed types for each method. The sheep, group and period did not differ significantly (P > 0.05) for feed degradation at most incubation times within any of these two methods. The in vitro method was able to mimic the in sacco degradation of feeds, although the absolute in vitro values were always lower than the in sacco values. The higher in sacco degradation for almost all feeds were partly attributed to the much greater losses of these feeds during their washing with water at 0 h. The in vitro method did identify significant differences (P < 0.05) in dry matter or crude protein degradations between and within different concentrates and grasses in parallel to the in sacco method. The ranking of feeds was comparable for these methods. The in vitro method can be used in parallel to the in sacco method to estimate degradation of various feeds. However, there is a need for coordinated studies to improve the prediction ability of the in vitro method for its adoption to routinely estimate the degradation of ruminant feeds.

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