scholarly journals Determination of soya protein in model meat products using image analysis

2011 ◽  
Vol 29 (No. 4) ◽  
pp. 318-321 ◽  
Author(s):  
Z. Randulová ◽  
B. Tremlová ◽  
Z. Řezáčová-Lukášková ◽  
M. Pospiech ◽  
I. Straka

The addition of plant proteins into meat products is nowadays a commonly used practice especially for the technological and economical reasons. Their properties have been known and used in meat products production for a long time. In the past, wheat protein or flour had been used most frequently, however, in these days they are being replaced by soya protein which has much more favourable properties in its use. Considering the possible misuse of raw materials of plant origin for the adulteration of meat products, the existence of highly sensitive and accurate procedures for their detection is needed including the determination of their content. Soya protein can be detected using various methods. In our work, an immunohistochemical method was used with image analysis for the quantification of soya protein. Model meat products with the addition of known amounts of soya protein in various forms were made for this experiment.

1970 ◽  
Vol 16 (6) ◽  
pp. 472-476 ◽  
Author(s):  
Elizabeth B Solow ◽  
L W Freeman

Abstract Sensitive or simple methods for the rapid determination of cholesterol in biological fluids have been developed during the past 10 years. Sensitivity has been increased by fluorimetry of the Lieberman—Burchard reaction for cholesterol. Measurement of the reaction of cholesterol with ferric chloride is simpler. Still, there are great differences between the results when different methods are used to measure the microquantities of cholesterol present in small volumes of serum or cerebrospinal fluid. In the proposed method, the simpler ferric chloride technique has been made highly sensitive by use of fluorometry. As little as 100 µl of cerebrospinal fluid, containing less than 1 µg of cholesterol, may be used, and the reaction is stable for as long as 1 h. Interference was negligible from pigments (such as bilirubin and hemoglobin), certain drugs, and ionic substances that might be expected to affect fluorescence.


2021 ◽  
Vol 14 (3(53)) ◽  
pp. 62-70
Author(s):  
Irina Vasilevna Kaltovich

The article presents the results of research on the determination of rational dosages for the use of emulsions from collagen-containing raw materials, which have undergone technological preparation, in meat products with various degrees of grinding of meat raw materials. It has been found that the optimal dosages for adding emulsions from pork skin and tails fermented by bacteria of the genus Lactobacillus (c (Lb.plantarum: Lb.casei (1:1)) = 1 Ч 107 CFU/g,? = 18 hours, t = 34 °C, 1:2 hydraulic module) to meat products, allowing to provide improved functional-technological and structural-mechanical parameters of these products, are as follows: for model stuffing systems from raw materials subjected to chopping during 8-12 minutes - up to 16%, 2-4 minutes - up to 14%, mincing with diameter of grid holes 2-3 mm - up to 12%, and from blanched raw materials subjected to chopping during 5-7 minutes - up to 20%. At the same time, emulsions from connective tissue fermented by bacteria of the genus Lactobacillus are recommended for use in meat products in the following rational dosages: up to 14% - for model stuffing systems from raw materials subjected to chopping for 8-12 minutes, up to 18% - from blanched raw materials subject to chopping for 5-7 minutes, up to 12% - from raw materials subject to chopping for 2-4 minutes, up to 10% - from raw materials subject to chopping with diameter of grid holes of 2-3 mm.


Author(s):  
A. V. Litvinova ◽  
N. S. Talalaeva ◽  
M. V. Parfenova

Import substitution is an integral part of the strategy to improve the competitiveness of the national agri-food complex. It should be a stimulus that will accelerate the economy and will contribute to the formation of a self-replicating mechanism of economic growth. The study analyzed the development of import substitution during the introduction of economic sanctions by Western countries and the United States. The study showed that the need for import substitution was brewing for a long time, however, the sanctions served as the impetus for its acceleration. The introduction by the President of the Russian Federation of special economic measures did not contradict the existing legislation and was aimed at restricting the importation of many types of products from abroad for the development of domestic production. The commodity structure of domestic exports and imports was also analyzed: the export of hydrocarbons has been prevailing for many years, but over the past two years their share in the total export structure has decreased. Most of the country imports machinery, equipment, vehicles, food and agricultural products, chemical products. For most indicators, there is a negative trend, due to which domestic imports decreased over the period of the sanctions by more than 100 billion US dollars. Despite economic sanctions, the European Union remains the main trading partner of Russia. Over the past 25 years, Russia has been a passive participant in international trade and, despite its high potential in the development of agriculture, was perceived by the world community as a country focused on the export of raw materials and hydrocarbons to a foreign market, rather than on the development of its own production, including agricultural production.


2020 ◽  
pp. 131-138
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Vladimirovna Sharova ◽  
Polina Valeriyevna Afanaseva

The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves. Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times. We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.


2021 ◽  
Author(s):  
Branislav Stojanović ◽  
Saša Janković ◽  
Vesna Đorđević ◽  
Sonja Marjanović ◽  
Dragan Vasilev ◽  
...  

Abstract This work aimed to examine the influence of the storage period on the content of toxic elements (As, Cd, Pb, and Hg), in five types of canned meat products, which are regularly used in the Serbian Armed Forces. Cans of beef goulash (BG), pork ragout (PR), spam (SP), liver pate (LP), and meatballs in tomato sauce (MB), produced according to military standards, and stored under regular conditions (temperature up to max 25 °C and relative humidity up to max 75%), were analyzed in this research. Meat products were made according to the special military requirements, packed in tinplate cans, and stored for up to 6 years. The highest average contents of toxic elements were found to be 10.00 µg/kg for arsenic in BG, 35.91 µg/kg for cadmium in LP, 15.04 µg/kg for mercury in PR, and 8.00 µg/kg for lead in PR. The storage period did not significantly affect the level of toxic elements, although higher concentrations were found in samples stored for more than two years. The influence of raw materials, spices, and additives on the level of toxic elements in some meat products was also examined. None of the samples contained toxic elements at levels exceeding the currently maximum permitted levels. The consumption of this type of food represents a small risk to human health because the exposure of soldiers to toxic elements, calculated as weekly intake, is far below legal PTWI/TWI limits, established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) and European Food Safety Authority (EFSA).


2021 ◽  
Vol 9 (16) ◽  
pp. 150-163
Author(s):  
Irіna Romanchuk ◽  
◽  
Kateryna Kopylova ◽  
Serhii Verbytskyi ◽  
Olha Kozachenko ◽  
...  

The subject of research is standardization as one of the forms of technical regulation in the food industry of Ukraine, in particular in the production of milk and milk products, meat and meat products, determination of safety and quality parameters of the specified food raw materials and finished products made from those, as well as installations and equipment for the food industry. The purpose of the study is to analyze the state of standardization in the production of dairy and meat products, highlight the existing problems and possible ways to solve them, as well as outline the role that the Technical Committee for Standardization 140 “Milk, meat and products of their processing” should play in these processes. Methods. During the research, a systematic approach was used to research factual materials, in particular scientific and scientific-practical literature, regulatory legal acts, regulatory documents and the like, abstract-logical approach to the synthesis of research results and the formulation of conclusions. The results of the study. National standards of Ukraine DSTU is the base of the technical regulation in relation to food products and methods of their control. The above also applies to standardization in the field of milk and meat products, which is within the competence scope of the Technical Committee for Standardization 140 “Milk, meat and products of their processing”. Technical Committee 140 carries out significant work to develop new and update existing national standards in accordance with its area of competence, in particular, it has significant success in harmonizing international and European standards. As of January 15, 2021, according to the classification code 67.100 (Milk and milk products) out of 203 standards of the nationwide force, 103 standards (50.7%) were developed by harmonizing the relevant international standards EN, ISO, etc. According to the classification code 67.120 (Meat, meat products and other animal products) – 202 units, 26 units and 14.1 %, respectively, according to the classification code 67.260 (Installations and equipment for the food industry) – 99 units, 86 units and 86.9%, respectively. The issue of attracting extra-budgetary funds for the harmonization of international standards and the implementation of the entire range of works on standardization is urgent. The issue of proper regulatory support for the production and operation of specialized technological equipment for food and processing industry enterprises is relevant, for which it is advisable to combine the domestic traditions of standardization with the priorities of safety and hygiene of machines, as well as ecology, which are in force in international practice. Scope of research results. The results of the studies performed will be used in the field of technical regulation, in particular, national standardization, of milk and milk products, meat and meat products, determination of safety and quality indicators of the specified food raw materials and finished products made from it, as well as installations and equipment for the food industry.


2020 ◽  
Vol 16 (5) ◽  
pp. 533-544
Author(s):  
Mohammad-Reza Afshar-Mogaddam ◽  
Adeleh Yadeghari ◽  
Abolghasem Jouyban

Background: Aristolochic acids are chemically linked to nitrophenanthrene carboxylic acids which are found in aristolochia plants. These compounds are intrinsically carcinogenic, while they have been used in traditional medicine from a long time ago. Despite the beneficial effects of herbals for treating some diseases, they possess some side effects. Methods: Therefore, the development of a sensitive and selective procedure for the determination of these harmful components in various complicated samples is an important task for health systems and drug authorities. In the past years, ultra-pressure liquid chromatography, high performance liquid chromatography and capillary electrophoresis with different detection systems were used for determination of aristolochic acids in various samples. Results: In this review, different analytical methods have been discussed in brief and applications of them in diverse samples have been summarized. Conclusion: Different approaches are compared from point of sensitivity, selectivity, and extraction efficiency.


2011 ◽  
Vol 20 (No. 5) ◽  
pp. 175-180 ◽  
Author(s):  
B. Tremlová ◽  
P. Štarha

In the framewort of the quantitative histologic evaluation of poultry products, the size and the number of bone fragments have been determined using the image analysis. Bone fragments were identified by their colour and analysed automatically. The samples contained 135 to 2167 bone particles the length of which varied from 5 to 2088 μm. Comparing products of the same kind, we found differences in the contents of bone fragments; this fact was possibly due to inadequate observance of the technological procedure by some producers.    


Author(s):  
V. Lyasota ◽  
◽  
N. Bogatko ◽  
N. Bukalova ◽  
L. Bogatko ◽  
...  

In connection with Ukraine's accession to the WTO, the Government has been tasked to take steps to ensure a consistent transition to the new International Requirements for the Safety and Quality of Raw Materials and Foodstuffs, including on animal health control. The purpose of the research was to carry out veterinary-sanitary examination of beef in the conditions of the agro-industrial market, to improve and develop express methods of determination of beef qualities of NOR- and PSE. The following research methods were used: organoleptic, biochemical, physicochemical, microscopic, microbiological, morphological and biometric. It is theoretically substantiated and experimentally confirmed the necessity to carry out veterinary and sanitary evaluation of beef qualities of NOR, PSE and DFD obtained from black-billed bull calves aged 24-27 months after maturation. The optimal quality indicators of beef quality NOR, PSE and DFD were determined. In order to prevent the loss of meat raw materials during processing, as well as in the production of meat products, it is experimentally and theoretically substantiated the need to determine the beef qualities of NOR- and PSE by organoleptic, morphological parameters, as well as by pH and pigment content. Use patented photometric methods for express and effective determination of NOR- and PSE beef in the state veterinary laboratory on the market. It is scientifically substantiated and experimentally proved the expediency of carrying out veterinary and sanitary evaluation of beef of different enterprises of Kyiv region in accordance with the current national standards and normative legal acts of Ukraine. Thus, a reliable quantitative method for determining the total content of pigments in beef qualities of NOR- and PSE along with other methods of determining these qualities (moisture retention capacity, pH, moisture content, organoleptic) is proposed. The method has the advantage over the existing methods of determining beef NOR- and PSE in that the results have a specific, reliable quantitative value. Methodical recommendations “Veterinary-sanitary evaluation of beef using quantitative method for determination of total pigment content in NOR- and PSE-meat of cattle” approved by the Academic Council of the Faculty of Veterinary Medicine of Bilotserkiv National Agrarian University, December 5, 2018 .


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