scholarly journals Psychrobacter immobilis isolated from foods: characteristics and identification

2015 ◽  
Vol 46 (No. 4) ◽  
pp. 95-100 ◽  
Author(s):  
Z. Páčová ◽  
E. Urbanová ◽  
E. Durnová
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Species Identification ◽  
Species Level ◽  
The Other ◽  
Identification System ◽  
Conventional Tests ◽  
Comamonas Terrigena ◽  
Correct Species Identification

A total of 15 strains of Psychrobacter immobilis isolated from animal sources, e.g. cheese, fish and poultry, were tested. A commercial diagnostic kit NEFERMtest 24, conventional tests and determination of fatty acids were used for identification. By using the results of NEFERMtest 24 and numerical identification system TNW version 6.0 the identification was successful on the species level (46.7%) while the correct species identification by using conventional tests increased up to 86.7%. All 9 saccharolytic strains including 7 Czech isolates were identified in most cases on an excellent or very good level by both methods based on biochemical reactions. On the other hand, the identification of 6 asaccharolytic strains was unsuccessful especially by NEFERMtest 24. While 4 asaccharolytic strains were identified correctly on the basis of conventional tests on species or genus level, incorrect identification on species level, for example Ralstonia paucula, Comamonas terrigena, Oligella ureolytica, Moraxella lincolnii andPsychrobacter phenylpyruvicus, was found by using NEFERMtest 24. Determination of fatty acid composition by MIDI System confirmed the species identification of 9 out of the 10 tested strains of P. immobilis and 1 tested strain of Psychrobacter sp.

scholarly journals Lipid composition of the brains of she-goat and castrated goat consumed in Ekiti State, Nigeria

2015 ◽  
Vol 50 (2) ◽  
pp. 153-162 ◽  
Author(s):  
EI Adeyeye ◽  
AJ Adesina
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linear Correlation ◽  
The Other ◽  
A Value ◽  
Significant Difference ◽  
Value Range ◽  
Goat Brain

The levels of fatty acids, phospholipids and sterols were determined in the brains of she-goat and castrated goat found in Ekiti State, Nigeria by gas chromatography. Results showed that the crude fat levels were 9.98 and 10.2 % in the brains of she-goat and castrated goat respectively. The fatty acid composition of she-goat and castrated goat brain shows that the SFA was 40.6 and 42.7 %, MUFA was 37.1 and 38.7 % and PUFA was 20.9 and 22.3% respectively. The other parameters of she-goat and castrated goat brain were found: PUFA/SFA, 0.490 and 0.548; MUFA/SFA, 0.869 and 0.953; n-6/n-3, 0.775 and 11.7; LA/ALA, 0.876 and 28.0; AA/DGLA, 6.05 and 17.4; EPA/DHA, 1.00 and 5.89 and EPSI (PUFA/MUFA), 0.564 and 0.575 respectively. Phospholipids were present in she-goat and castrated goat with a value range of 2365 and 3047 mg/100g respectively. Among the sterols, only cholesterol was of any significant level with values of 1353 mg/100g (she-goat brain) and 1355 mg/100g (castrated goat brain). Linear correlation at ? = 0.05, df: n-1 showed that no significant difference exists between the crude fats, phospholipids and sterols except in the fatty acids parameters.Bangladesh J. Sci. Ind. Res. 50(2), 153-162, 2015

scholarly journals Fatty acid composition of night-scented stock (Matthiola bicornis (Sibth. & Sm.) DC.) raw materials

2021 ◽  
Vol 34 (1) ◽  
pp. 34-41
Author(s):  
Viktoriia O. Pinkevych ◽  
Moeen F. Dababneh ◽  
Nadiia Ye. Burda ◽  
Iryna O. Zhuravel
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Raw Materials ◽  
Similar Fatty Acid Composition ◽  
Domestic Plant ◽  
Plant Sources

Abstract Introduction. With due consideration of the properties of fatty acids, as well as their importance for normal life activity and human development, research into the fatty acid composition of poorly studied plants and the search for new domestic plant sources of polyunsaturated fatty acids is a mainstream trend in modern pharmacy. Aim. Aim of research – determination of fatty acid qualitative composition and content in threshed grass, stalks, roots and seeds of Night-scented stock ‘Queen of Night’ and ‘Evening Scent’ cultivars as grown in Ukraine. Methods. Gas chromatography. Results. Both cultivars of Night-scented stock taken for analysis had similar fatty acid composition – 5 saturated, 5 (4 for seeds) monounsaturated and 2 polyunsaturated fatty acids, Quantitatively, in all tested parts of the herb polyunsaturated and monounsaturated fatty acid dominated, making in total 88.92% and 88.62% in the seeds of Queen of Night and Evening Scent cultivars, respectively, and averaging 65% in other parts of the tested cultivars. Linolenic and linoleic acids prevailed among the polyunsaturated fatty acids, whereas oleic acid prevailed among the monounsaturated. Conclusion. Night-scented stock can be utilized as a source of polyunsaturated fatty acids for the development of drugs and for standardization of tested raw materials.

scholarly journals Determination of Whole-Cell Fatty Acid Profiles for the Characterization and Differentiation of Isolates of Rhizoctonia solani AG-4 And AG-7

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 785-788 ◽  
Author(s):  
R. E. Baird ◽  
R. D. Gitaitis ◽  
D. E. Carling ◽  
S. M. Baird ◽  
P. J. Alt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rhizoctonia Solani ◽  
Euclidean Distance ◽  
Methyl Esters ◽  
Principal Component ◽  
Identification System ◽  
Microbial Identification ◽  
Euclidean Distances

Fatty acid methyl esters (FAMEs) of isolates of Rhizoctonia solani AG-4 and AG-7 were characterized by gas chromatography and analyzed with Microbial Identification System software. Palmitic, stearic, and oleic acids were common in all isolates from both anastomosis groups (AGs) and accounted for 95% of the C14 to C18 fatty acids present. Oleic acid, most common in both R. solani AG-4 and AG-7 isolates, accounted for the greatest percentages of total FAMEs. The presence, quantities, or absence of individual fatty acids could not be used for distinguishing AG-4 and AG-7 isolates. Anteisopentadecanoic and 9-heptadecanoic acids, however, were specific to all three AG-7 isolates from Japan but absent in other AG-7 isolates and all AG-4 isolates. Pentadecanoic acid occurred in only two of the R. solani AG-4 isolates, but was not found in any of the AG-7 isolates. The AG-4 isolates could be distinguished from AG-7 isolates when quantities of FAMEs and key FAME ratios were analyzed with cluster analysis and principle components were plotted. Isolates of AG-7 from Arkansas, Indiana, and Georgia appeared to be more closely related to each other than to AG-7 isolates from Japan and Mexico. These differences in FAMEs were sufficiently distinct that isolate geographical variability could be determined. A dendrogram analysis cluster constructed from the FAMEs data showed results similar to that of the principal component analysis. Euclidean distances of total AG-4 isolates were distinct from total AG-7 isolates. The Arkansas and Indiana AG-7 isolates had a similar Euclidean distance to each another but the percentages were different for the AG-7 isolates from Japan and Mexico. In conclusion, variability of the FAMEs identified in this study would not be suitable as the main diagnostic tool for distinguishing individual isolates of R. solaniAG-4 from AG-7.

A Simplified Halphen Procedure for Cyclopropene Fatty Acids

1972 ◽  
Vol 55 (6) ◽  
pp. 1288-1293
Author(s):  
Eugene C Coleman ◽  
David Firestone
Keyword(s):  
Fatty Acids ◽  
Quantitative Determination ◽  
Closed System ◽  
Fats And Oils ◽  
The Other ◽  
Solvent Systems ◽  
Cyclopropene Fatty Acids ◽  
Reaction Media ◽  
Qualitative Analyses

Abstract A simplified Halphen procedure was developed for the quantitative determination of cyclopropene fatty acids in fats and oils. Butanol, dimethyl sulfoxide (DMSO), and mixtures of butanol and DMSO were used as reaction media in a closed system. Higher sample absorbances were produced in butanol than in mixtures of butanol and DMSO. Butanol was superior to the other solvent systems for both quantitative and qualitative analyses. The lower limit of sensitivity for the tube-butanol system for quantitative and qualitative analyses was 18 and 15 μg cyclopropene fatty acids/g oil, respectively.

Effect of storage of plasma and serum on enzymatic determination of non-esterified fatty acids

2001 ◽  
Vol 38 (3) ◽  
pp. 252-255 ◽  
Author(s):  
Luis García Menéndez ◽  
Ana L Fernández ◽  
Alfredo Enguix ◽  
Constanza Ciriza ◽  
Juan Amador
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Storage Conditions ◽  
The Other ◽  
Specific Reference ◽  
Enzymatic Method ◽  
Esterified Fatty Acids ◽  
Enzymatic Determination ◽  
The Stability

Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated plasma and serum under different temperature conditions. We conclude that EDTA-treated plasma and serum can both be used for analysis. Specific reference values should be established depending on the type of sample chosen. Samples that cannot be analysed immediately can be stored at -20°C for at least 14 days without significant changes in the concentration of total non-esterified fatty acids. None of the other storage conditions and periods studied are suitable for the measurement of non-esterified fatty acid concentration.

Species identification of staphylococci and micrococci isolated from ewes' milk cheeses

1988 ◽  
Vol 55 (2) ◽  
pp. 269-276 ◽  
Author(s):  
María Camino García ◽  
Andrés Otero ◽  
María Luisa García ◽  
María Rosario García ◽  
Benito Moreno
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Species Level ◽  
The Other ◽  
Sensitive Species ◽  
Coagulase Negative Staphylococci ◽  
Resistant Strains

SummaryForty-five strains of staphylococci and 6 strains of micrococci isolated from pasteurized ewes' milk cheeses (Manchego and Burgos varieties) were identified to species level. Three strains of staphylococci were identified asStaphylococcus aureus. Amongst the 42 coagulase-negative staphylococci, 8 were novobiocin-sensitive and 34 novobiocin-resistant. The novobiocin-sensitive species found wereStaph. auricularis(5 strains),Staph. caseolyticus(2 strains) andStaph. epidermidis(1 strain). Novobiocin-resistant strains were identified asStaph. gallinarum(5 strains),Staph. xylosus(4),Staph. saprophyticus(9),Staph. cohnii(6),Staph. sciuri(1) andStaph. lentus(3). The other 6 isolates could not be identified. Only 3 strains of micrococci were identified (Micrococcus kristinae, M. variansandM. sedentarius). The possible origin of the isolates is discussed.

scholarly journals Fatty acid and sodium contents of commercial milk chocolate – analytical aspects and nutritional information

2015 ◽  
Vol 18 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Renato Cesar Susin ◽  
Renata Mior ◽  
Vera Maria da Costa Dias
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Sodium Content ◽  
The Other ◽  
Porto Alegre ◽  
Milk Chocolate ◽  
Nutritional Information ◽  
Commercial Milk ◽  
Nutritional Facts

SummaryChocolate consumption is usually associated with enjoyment, milk chocolate desserts being a very popular choice. Besides, the literature provides data suggesting health benefits for chocolate products as compared to non-chocolate candies. However, the lipid composition of cocoa and its commercial products has yet to be completely elucidated and understood, although much research has been carried out with this objective. Contributions to this objective frequently face difficulties in the field of Analytical Chemistry due to the complexity of the composition of such a food. On the other hand, the sodium content of foods is currently a major concern. Thus, this work aims to provide information concerning the composition of commercial milk chocolate in terms of its fatty acid profile and sodium content. To achieve this purpose, analytical adjustments and improvements to the methodology were made and described in this paper. Sodium (FAAS) and a total of 50 fatty acids (GC-FID) were determined in eight samples of milk chocolate bars from different manufacturers. The samples were purchased from retailers in Porto Alegre – Brazil. In the determination of the fatty acids, possible losses during methylation deserved special attention and were studied. Nevertheless, large differences were not found in comparison with the nutritional facts declared on the label. However, the results obtained for sodium demonstrated the importance of food inspection, considering the discrepancies found.

Evaluation of cooling strategies for pumping of milk – Impact of fatty acid composition on free fatty acid levels

2005 ◽  
Vol 72 (4) ◽  
pp. 476-481 ◽  
Author(s):  
Lars Wiking ◽  
Hanne C Bertram ◽  
Lennart Björck ◽  
Jacob H Nielsen
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Milk Fat ◽  
Saturated Fat ◽  
The Other ◽  
Fat Globules ◽  
Milk Fat Globules

Cooling strategies for pumping of raw milk were evaluated. Milk was pumped for 450 s at 31 °C, or pumped after cooling to 4 °C and subsequently subjected to various incubation times. Two types of milk were used; i.e. milk from cows fed a diet high in saturated fat supplements resulting in significantly larger milk fat globules than the other type of milk which comes from cows fed a low-fat diet that stimulates high de novo fat synthesis. The content of liquid fat was determined by low-field 1H NMR, which showed that milk from cows given the saturated fat diet also contained less liquid fat at both 4 ° and 31 °C than the other type of milk. This can be ascribed to the differences in the fatty acid composition of the milk as a result of the fatty acid composition of the diets. After pumping of the milk at 31 °C, measurement of fat globule size distribution revealed a significant coalescence of milk fat globules in the milk obtained from the saturated fat diet due to pumping. Pumping at 4 °C or pumping the other type of milk did not result in coalescence of milk fat globules. Formation of free fatty acids increased significantly in both types of milk by pumping at 31 °C. Cooling the milk to 4 °C immediately before pumping inhibited an increased content of free fatty acids. However, when the milk was incubated at 4 °C for 60 min after cooling and then subjected to pumping, a significant increase in the formation of free fatty acids was observed in both types of milk. It is suggested that this increase in free fatty acids is caused by transition of polymorphic crystal forms or higher level of attached lipoprotein lipases to the milk fat globule before pumping.

Analysis of Fatty Acid Composition of Thevetia peruviana and Hura crepitans Seed oils using GC-FID

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
K A Alabi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oils ◽  
Gas Chromatograph ◽  
Thevetia Peruviana ◽  
Hura Crepitans ◽  
Very High

Fatty acids were extracted from Thevetia peruviana and Hura crepitans seed oils. The quantitative determination of fatty acid composition was carried out for each sample by methylation and application of gas chromatograph (GC-FID). The predominant fatty acids in the T. peruviana and H. crepitans are Palmitic acid (19.10% and 21.67%), Stearic acid (7.31% and 9.66%), Oleic acid (53.40% and 26.91%) and Linoleic acid (19.03% and 36.61%) respectively. In the two oils, the ratios of unsaturated fatty acid were very high about 73% for T. peruviana and 64% for H. crepitans which is an indication that both would be very good sources of oleochemicals for polymer and other industries.

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