Protein metabolism in the organism of growing borrows at the addition of 20-hydroxyexdisone to the diet

2020 ◽  
Vol 3 ◽  
pp. 158-162
Author(s):  
O.V. Obvintseva ◽  
◽  
K.T. Erimbetov ◽  
A.G. Solovyova ◽  
V.V. Mikhailov ◽  
...  
Keyword(s):  
Protein Metabolism ◽  
Crude Protein ◽  
The Body ◽  
Biologically Active ◽  
Nitrogen Excretion ◽  
Back Ground ◽  
Limiting Amino Acid ◽  
Experimental Group ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

One of the approaches to the creation of biologically active additives in pig nutrition can be the use of phytopreparations, in par-ticular, 20-hydroxyecdysone (20-GE), which regulates protein metabolism in piglets. The aim of this work is to assess the effect of 20-GE on the metabolism of proteins in the body and to measure their turnover in grow-ing boars. The experiment was carried out on boars (♂ Danish Yorkshire × ♀ Danish Landrace) from 60 to 120 days of age. At the age of 60 days, boars were divided into 2 groups: control and experimental, fed with feed with a content (g / kg) of crude protein 158.7, lysine 7.7, threonine 4.8, methionine 4.6, exchange energy (EE) 12.7 MJ / kg. The ratio of the first limiting amino acid lysine to EE was 61%. The boars of the experimental group were injected with 20-GE at a dose of 1.6 mg / kg of body weight. In boars of the experimental group, compared with the con-trol, there was a decrease in urinary nitrogen excretion (by 26.8%, P≤0.05). Nitrogen re-tention was higher in boars of the experi-mental group by 19.0% (P≤0.001) compared to the control. The results of studies on pro-tein turnover obtained using a stable isotope indicate that the anabolic effect of 20-GE isdetermined by an increase in the rate of pro-tein synthesis at the same rates of their deg-radation, nitrogen flow against the back-ground of a decrease in its endogenous loss-es. 20-GE by activating a signal through protein kinase B provides an increase in bio-synthetic processes at the stage of transla-tion. It was concluded that the use of 20-GE in boars cultivation increases the efficiency of using amino acids for the synthesis and deposition of proteins in the body.

Partition of nitrogen in the urine of African sheep given a variety of low-protein diets

1967 ◽  
Vol 9 (2) ◽  
pp. 219-227 ◽  
Author(s):  
J. H. Topps ◽  
R.C. Elliott
Keyword(s):  
Crude Protein ◽  
The Body ◽  
Nitrogen Excretion ◽  
Crude Protein Content ◽  
Positive Correlation ◽  
Low Protein ◽  
Nitrogen Intake ◽  
Protein Diets ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

1. Sixteen foods, made up with differing proportions of roughage and concentrates and ranging in crude protein content from approximately 2·6% to 10·0%, were each offered ad libitum to Blackhead Persian wether sheep. The nitrogen balance of the sheep and the distribution of nitrogen in the urine were measured.2. Urinary nitrogen excretion decreased with the digestible nitrogen intake. There was a highly significant (P < 0·01) positive correlation between either excretion of urea or of allantoin and digestible nitrogen intake.3. The amount of creatinine excreted varied little with diet but was directly related to the body weight of the sheep.4. For 8 diets, hippurate excretion by the sheep was directly related (P<0·01) to intake of digestible nitrogen, while a significant (P<0·05) positive correlation between the same measures was found for the other 8 diets.5. It is suggested that with certain types of diets the excretion of either nitrogen, urea or allantoin in the urine may be useful indicators of digestible nitrogen intake of low-protein diets by sheep.

The effect of ERDP:FME ratio and DUP supply from concentrates on the performance of lactating ewes offered grazed grass

1996 ◽  
Vol 1996 ◽  
pp. 160-160
Author(s):  
R.G. Wilkinson ◽  
L.A. Sinclair ◽  
J. Powles ◽  
C.M. Minter
Keyword(s):  
Crude Protein ◽  
Microbial Growth ◽  
High Rate ◽  
Nitrogen Excretion ◽  
Crude Protein Content ◽  
Nitrogen Utilisation ◽  
Metabolisable Energy ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen ◽  
Degradable Protein

Fresh grass is a highly variable product, which is generally characterised as having a high crude protein content, with a high rate and extent of degradation in the rumen. For lactating ruminants, this may result in an effective rumen degradable protein: fermentable metabolisable energy (ERDP:FME) ratio in excess of the optimum requirement for microbial growth, leading to inefficient nitrogen utilisation and high levels of urinary nitrogen excretion. One method of improving the efficiency of nitrogen utilisation is the use of supplements. An appropriate supplement should optimise ERDP and FME supply to the rumen and provide sufficient metabolisable protein (MP) to balance animal requirements. The objective of the experiment was to investigate the response of lactating ewes offered grazed grass to variations in ERDP:FME ratio and digestible undegradable protein (DUP) supply from concentrate supplements.

The effect of ERDP:FME ratio and DUP supply from concentrates on the performance of lactating ewes offered grazed grass

1996 ◽  
Vol 1996 ◽  
pp. 160-160
Author(s):  
R.G. Wilkinson ◽  
L.A. Sinclair ◽  
J. Powles ◽  
C.M. Minter
Keyword(s):  
Crude Protein ◽  
Microbial Growth ◽  
High Rate ◽  
Nitrogen Excretion ◽  
Crude Protein Content ◽  
Nitrogen Utilisation ◽  
Metabolisable Energy ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen ◽  
Degradable Protein

Fresh grass is a highly variable product, which is generally characterised as having a high crude protein content, with a high rate and extent of degradation in the rumen. For lactating ruminants, this may result in an effective rumen degradable protein: fermentable metabolisable energy (ERDP:FME) ratio in excess of the optimum requirement for microbial growth, leading to inefficient nitrogen utilisation and high levels of urinary nitrogen excretion. One method of improving the efficiency of nitrogen utilisation is the use of supplements. An appropriate supplement should optimise ERDP and FME supply to the rumen and provide sufficient metabolisable protein (MP) to balance animal requirements. The objective of the experiment was to investigate the response of lactating ewes offered grazed grass to variations in ERDP:FME ratio and digestible undegradable protein (DUP) supply from concentrate supplements.

scholarly journals Reduction of crude protein in diets fed to lactating Holstein-Gyr cows

2018 ◽  
Vol 53 (7) ◽  
pp. 858-865
Author(s):  
Clóvis Ribeiro Guimarães ◽  
Rafael Alves de Azevedo ◽  
Mariana Magalhães Campos ◽  
Fernanda Samarini Machado ◽  
Alexandre Mendonça Pedroso ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
Nitrogen Excretion ◽  
Productive Performance ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

Abstract: The objective of this work was to evaluate the effect of reducing crude protein (CP) contents in diets with a constant metabolizable protein content on the intake, performance, nitrogen balance, and nutrient digestibility of lactating Holstein-Gyr cows. Animals (n = 24, 103±23 days in milk) were allocated to four treatments (n = 6 per group) with different CP contents: 127, 132, 139, and 156 g kg-1 dry matter (DM). DM intake was not affected by treatments. CP intake and digestibility increased linearly with higher CP contents. Milk yield (23.7±3 kg per day) and the percentages of milk protein (3.3±0.2%) and fat (3.8±0.5%) were not affected by CP reduction. Milk and blood urea nitrogen increased linearly with the increase of CP in the diet, similarly to urinary nitrogen excretion. Nitrogen use efficiency was 29.8 and 22.4% when CP was 127 and 156 g kg-1 DM, respectively. Reducing CP in diets fed to mid-lactating Holstein-Gyr cows increases nitrogen use efficiency and maintains the productive performance of the cows.

Hyperglucagonemia during insulin deficiency accelerates protein catabolism

1987 ◽  
Vol 253 (2) ◽  
pp. E208-E213 ◽  
Author(s):  
K. S. Nair ◽  
D. Halliday ◽  
D. E. Matthews ◽  
S. L. Welle
Keyword(s):  
Protein Metabolism ◽  
Nitrogen Excretion ◽  
Insulin Deficiency ◽  
Leucine Oxidation ◽  
Precise Role ◽  
Combined Deficiency ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

Hyperglucagonemia coexists with insulin deficiency or insulin resistance in many conditions where urinary nitrogen excretion is increased, but the precise role of glucagon in these conditions is controversial. The purpose of this study was to evaluate the effect of hyperglucagonemia on protein metabolism in insulin-deficient subjects. We used the stable isotope of an essential amino acid (L-[1-13C]leucine) as a tracer of in vivo protein metabolism. A combined deficiency of insulin and glucagon was induced by intravenous infusion of somatostatin. Hyperglucagonemia and hypoinsulinemia were induced by infusions of somatostatin and glucagon. When somatostatin alone was infused leucine flux increased, indicating a 6-17% increase in proteolysis. When somatostatin and glucagon were infused, leucine flux increased, indicating a 12-32% increase in proteolysis. The increase in leucine flux during the infusion of somatostatin and glucagon was higher than the increase during infusion of somatostatin alone. Somatostatin alone did not change leucine oxidation, whereas the somatostatin plus glucagon increased leucine oxidation 100%. We conclude that hyperglucagonemia accelerates proteolysis and leucine oxidation in insulin-deficient humans.

scholarly journals The fasting metabolism of cattle

1966 ◽  
Vol 20 (1) ◽  
pp. 103-111 ◽  
Author(s):  
K. L. Blaxter ◽  
F. W. Wainman
Keyword(s):  
Carbon Dioxide ◽  
Body Weight ◽  
Oxygen Consumption ◽  
Coefficient Of Variation ◽  
Methane Production ◽  
Carbon Dioxide Production ◽  
The Body ◽  
Nitrogen Excretion ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

1. The metabolism of seventeen steers was determined on forty-nine occasions during fasts of either 112 or 136 h duration.2. Faeces continued to be produced during fasts of up to 136 h duration at rates which were 15–20% of those noted before the fasts began.3. Carbon dioxide production and oxygen consumption fell continuously throughout with animals weighing less than 200 kg but changed little after 88 h in animals weighing more than 200 kg. Methane production was considerably reduced during fasting but did not disappear. Urinary nitrogen excretion changed very little. Of the total loss of energy from the body, the loss of protein accounted for 25%. This was unaffected by age or size of animal.4. With individual Ayrshire steers, metabolism increased during growth with body-weight raised to the power 0.68±0.05. No greater precision of estimate was obtained from logarithmic regressions of metabolism on body-weight than from linear ones.5. Seven Ayrshire steers had a mean fasting metabolism of 100±1.6 kcal/kg W0.73 24 h, eight Black cattle of the Aberdeen Angus type a fasting metabolism of 81±1.5 kcal/kg W0.73 24 h and two Ayrshire x Beef Shorthorn steers a fasting metabolism of 96±2.9 kcal/kg W0.73 24 h. Variation in the fasting metabolism of an individual steer from time to time, expressed as a coefficient of variation, was ±7.4%.6. The results are discussed in relation to interspecies generalizations about the relation between fasting metabolism and body-weight.

scholarly journals Effects of dietary 20-hydroxyecdysone supplementation on whole-body protein turnover in growing pigs

2020 ◽  
Vol 175 ◽  
pp. 03008
Author(s):  
Olga Obvintseva ◽  
Kenes Erimbetov ◽  
Vitaly Mikhailov
Keyword(s):  
Protein Metabolism ◽  
Protein Turnover ◽  
Live Weight ◽  
Growing Pigs ◽  
The Body ◽  
Biologically Active ◽  
Whole Body ◽  
Body Protein ◽  
Protein Deposition ◽  
Experimental Group

One of the approaches to creating biologically active additives for use in pig breeding can be the use of 20-hydroxyecdysone regulating protein metabolism in piglets. The purpose of the work is to assess the effect of 20-hydroxyecdysone on turnover of protein in piglets. The experiment was carried out on barrows (♂ Danish Yorkshire × ♀ Danish landrace) to achieve a live weight of 53-62 kg. At the age of 60 days, 2 groups of piglets were formed: control and experimental. Piglets of the experimental group were injected with 20-hydroxyecdysone at a dose of 1.6 mg / kg body weight. In piglets of the experimental group, in comparison with the control, a decrease in the excretion of nitrogen in the urine was noted (by 26.8%, P <0.05). Nitrogen deposition was higher in piglets of the experimental group by 19.0% (P <0.001) compared with the control. 20-hydroxyecdysone contributed to increased protein deposition in the body of piglets due to protein synthesizing activity. Thus, the use of 20-hydroxyecdysone in pigs increases the efficiency of using amino acids for the synthesis and deposition of proteins in the body.

scholarly journals Nutritive value of Eurolysine bacterial protein and Pekilo protein for growing pigs

1982 ◽  
Vol 54 (4) ◽  
pp. 263-269
Author(s):  
Matti Näsi
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Nitrogen Retention ◽  
Growing Pigs ◽  
Nitrogen Excretion ◽  
Ether Extract ◽  
Bacterial Protein ◽  
Balance Trials ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

Digestibility and balance trials were performed with six growing pigs, to evaluate the nutritive value and protein utilization of Eurolysine bacterial protein and Pekilo protein used to replace 50 % or 100 % of soybean supplement in a barley-based diet. Eurolysine had a high content of crude protein, 67.8 % of DM, and was rich in lysine, 8.3 g/16 N, and the corresponding values for Pekilo were 49.5 % and 5,5 g/16 g N. The diets containing Eurolysine had lower digestibilities than those with Pekilo or soybean meal. With Eurolysine the digestibility values for crude protein, ether extract and N-free extract (NFE) were, respectively, 64.3 %, 35.4 % and 76.5 %, and with Pekilo 74.8 %, 28.5 % and 79.4 %. The feed values for Eurolysine were 0.82 FU/kg, 509 g DCP/kg and 12.0 MJ ME/kg DM, and those for Pekilo 0.90 FU/kg, 409 g DCP/kg and 12.6 MJ ME/kg DM. The urinary nitrogen excretion was lower with Eurolysine than with the other supplements, and the nitrogen retention and the biological values were higher (P < 0.05). Eurolysine is well suited as a protein supplement in pig feeding because of its high content of lysine. Combined with barley the protein in Eurolysine gave a high biological value.

Influences of diet and body condition on the nitrogen utilization in the cow

1972 ◽  
Vol 79 (2) ◽  
pp. 323-329 ◽  
Author(s):  
R. Paquay ◽  
R. De Baere ◽  
A. Lousse
Keyword(s):  
Body Condition ◽  
Nitrogen Balance ◽  
Live Weight ◽  
The Body ◽  
Metabolizable Energy ◽  
Nitrogen Excretion ◽  
Maintenance Requirements ◽  
Energy Maintenance ◽  
Urinary Nitrogen Excretion ◽  
Urinary Nitrogen

SUMMARYThe dietary and individual factors influencing the utilization of digestible nitrogen in the dry cow were studied with numerous rations fed to Friesian cows kept in metabolism stalls.Urinary nitrogen excretion and nitrogen balance were above all influenced by the level of digestible nitrogen intake. They are also correlated to metabolizable energy and digestible potassium intakes.Endogenous urinary nitrogen, estimated by extrapolation, was 30·6 mg N/kg body weight/d or 0·169 W0·73 g/d with the rations meeting approximately the energy maintenance requirements of the cow.The changes in diet accounted for 80% of the variability of nitrogen balance.A method was worked out for calculating the body condition of the cow from the live-weight variations.The changes in body condition accounted for 11% of the variability of nitrogen balance.

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