scholarly journals DEVELOPMENT OF POLYMERIC MICROPARTICLES WITH RADACHLORINE AND ESTIMATION OF THE PROSPECTS OF THEIR USE IN PHOTODYNAMIC THERAPY

2021 ◽  
Vol 20 (3) ◽  
pp. 47-56
Author(s):  
A. M. Miroshkina ◽  
S. P. Krechetov ◽  
N. L. Solovieva ◽  
I. I. Krasnyuk
Keyword(s):  
Electron Microscopy ◽  
Photodynamic Therapy ◽  
Magnetic Nanoparticles ◽  
Tumor Cells ◽  
Double Emulsion ◽  
Significant Inhibition ◽  
Polymeric Microparticles ◽  
Polymer Microparticles ◽  
Local Use ◽  
Spherical Microparticles

Introduction. Nowadays, the development of delivery systems based on micro- and nanoparticles is being actively pursued to increase the selectivity and efficiency of photosensitizers in photodynamic therapy. Such microparticles could increase the effectiveness of the already used chemotherapeutic drugs due to their accumulation in the tumor and help to overcome the drug resistance of tumor cells.The aim of this research was to obtain microparticles based on a biocompatible block copolymer of lactic and glycolic acids with the inclusion of the photosensitizer radachlorin, magnetic nanoparticles, and perfluorodecalin and their subsequent evaluation as therapeutic agents for photodynamic therapy.Materials and methods. Microparticles were obtained using the double emulsion method, described using of electron microscopy. Evaluation of their photodynamic properties was carried out using spectrophotometry and MTTtest on cell culture.Results. Spherical microparticles with a size of less than 1 μm were obtained. The release of the active substance from microparticles occurred gradually over two weeks, and in the case of the presence of magnetic nanoparticles, the concentration of radachlorin remained practically unchanged for a month. Exposure of microparticles to the light of LED is accompanied by the formation ofsinglet oxygen. Electron microscopy indicated intracellular position of microparticlesin tumor cells. The MTT test revealed a significant inhibition of cell viability in the presence of microparticles.Conclusion. The research results allow us to consider the obtained biocompatible polymer microparticles with the inclusion of radachlorin as a depot of radachlorin for local use in photodynamic therapy of tumors. 

DEVELOPMENT OF NANOSTRUCTURED POLYMERIC MICROPARTICLES WITH RADACHLORINE AS A COMPLEX DELIVERY SYSTEM FOR PHOTODYNAMIC THERAPY

2020 ◽  
pp. 160-163
Author(s):  
S. Krechetov ◽  
M. Yakovcheva ◽  
A. Babenechev ◽  
I. Krasnyk
Keyword(s):  
Photodynamic Therapy ◽  
Magnetic Nanoparticles ◽  
Singlet Oxygen ◽  
Delivery System ◽  
Active Substance ◽  
Light Radiation ◽  
Multiple Emulsion ◽  
Polymeric Microparticles

Polymeric biocompatible microparticles based on a copolymer of lactic and glycolic acids with the inclusion of magnetic nanoparticles, perfluorodecalin and radachlorin as an active substance were obtained by the method of multiple emulsion. It has been shown that the obtained microparticles have a sufficient inclusion of radachlorin to generate singlet oxygen and manifest a photocytotoxic effect when exposed to light radiation used in photodynamic therapy.

Optical Properties of Photodynamic Therapy Drugs in Different Environments: The Paradigmatic Case of Temoporfin

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Optical Properties ◽  
Photodynamic Therapy ◽  
Minimally Invasive ◽  
Visible Light ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Molecular Oxygen ◽  
Computational Tools

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

scholarly journals BSA-encapsulated cyclometalated iridium complexes as nano-photosensitizers for photodynamic therapy of tumor cells

RSC Advances ◽  
2021 ◽  
Vol 11 (25) ◽  
pp. 15323-15331
Author(s):  
Yao Xu ◽  
Xiang Wang ◽  
Kang Song ◽  
Jun Du ◽  
Jinliang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Iridium Complexes

Three new iridium complexes were synthesized and fabricated with BSA to form nano-photosensitizers, which can catalyze oxygen to produce singlet oxygen to achieve photodynamic therapy of tumor cells.

Chiral Alkyl Groups at Position 3(1′) of Pyropheophorbide-a Specify Uptake and Retention by Tumor Cells and Are Essential for Effective Photodynamic Therapy

2021 ◽  
Vol 64 (8) ◽  
pp. 4787-4809
Author(s):  
Ravindra R. Cheruku ◽  
Erin C. Tracy ◽  
Walter Tabaczynski ◽  
Joseph R. Missert ◽  
Heinz Baumann ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Cells ◽  
Alkyl Groups ◽  
Pyropheophorbide A

scholarly journals Three-dimensional relationships between tumor cells and microcirculation with double cyanine immunolabeling, laser scanning confocal microscopy, and computer-assisted reconstruction: an alternative to cast corrosion preparations.

1994 ◽  
Vol 42 (5) ◽  
pp. 681-686 ◽  
Author(s):  
V Rummelt ◽  
L M Gardner ◽  
R Folberg ◽  
S Beck ◽  
B Knosp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Vessels ◽  
Tumor Cells ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Melanoma Cells ◽  
Tumor Blood Vessels ◽  
The Relationship ◽  
Scanning Electron

The morphology of the microcirculation of uveal melanomas is a reliable market of tumor progression. Scanning electron microscopy of cast corrosion preparations can generate three-dimensional views of these vascular patterns, but this technique sacrifices the tumor parenchyma. Formalin-fixed wet tissue sections 100-150 microns thick from uveal melanomas were stained with the lectin Ulex europaeus agglutinin I (UEAI) and proliferating cell nuclear antigen (PCNA) to demonstrate simultaneously the tumor blood vessels and proliferating tumor cells. Indocarbocyanine (Cy3) was used as a fluorophore for UEAI and indodicarbocyanine (Cy5) was used for PCNA. Double labeled sections were examined with a laser scanning confocal microscope. Images of both stains were digitized at the same 5-microns intervals and each of the two images per interval was combined digitally to form one image. These combined images were visualized through voxel processing to study the relationship between melanoma cells expressing PCNA and various microcirculatory patterns. This technique produces images comparable to scanning electron microscopy of cast corrosion preparations while permitting simultaneous localization of melanoma cells expressing PCNA. The microcirculatory tree can be viewed from any perspective and the relationship between tumor cells and the tumor blood vessels can be studied concurrently in three dimensions. This technique is an alternative to cast corrosion preparations.

scholarly journals Comparison of select cancer biomarkers in human circulating and bulk tumor cells using magnetic nanoparticles and a miniaturized micro-NMR system

2013 ◽  
Vol 9 (7) ◽  
pp. 1009-1017 ◽  
Author(s):  
Arezou A. Ghazani ◽  
Shaunagh McDermott ◽  
Melina Pectasides ◽  
Matt Sebas ◽  
Mari Mino-Kenudson ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Tumor Cells ◽  
Cancer Biomarkers

Adrenal Cortical Adenoma With Adrenalin-Type Neurosecretory Granules Clinically Mimicking a Pheochromocytoma

2002 ◽  
Vol 126 (12) ◽  
pp. 1530-1533 ◽  
Author(s):  
Tomislav Ivsic ◽  
Richard A. Komorowski ◽  
Gary S. Sudakoff ◽  
Stuart D. Wilson ◽  
Milton W. Datta
Keyword(s):  
Electron Microscopy ◽  
Clinical Outcome ◽  
Tumor Cells ◽  
Clinical Features ◽  
Adrenal Tumor ◽  
Histologic Examination ◽  
Adrenal Tumors ◽  
Neurosecretory Granules ◽  
Adrenal Cortical Adenoma ◽  
Pathologic Features

Abstract Adrenal tumors often present with clinical features that are specific and unique to their endocrine metabolism. When these features are in conflict with the pathologic appearance of the tumor, there can be great consternation for both the pathologist and the surgeon. In the case reported herein, an adrenalectomy was performed for clinical features of pheochromocytoma that on gross and histologic examination had the pathologic features of an adrenal cortical adenoma. Electron microscopy subsequently revealed that the tumor cells contained adrenalin-type granules, explaining the clinical outcome. It is crucial for both the surgeon and the surgical pathologist to be aware of this possibility when the clinical and pathologic features of an adrenal tumor are not congruent.

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