Efficacy of Platelet-Rich-Plasma (PRP) in Bone Regeneration after Cyst Enucleation in Pediatric Patients – A Clinical Study

Background: Platelet-rich-plasma (PRP) is a type of natural source of autologous growth factors, and has been used successfully in various fields. However, the use of PRP in children is not well documented. Objective:To evaluate the efficacy of adding PRP to a bone graft in the bone regeneration of cystic bony defects following cystectomy. Study design: Study sample included 20 children who were randomly divided into two groups with 10 patients in each group; all underwent cystectomy. In the first group (test group), after cystectomy the cystic defect was filled with PRP and bone graft. In the second group (control group) bone graft alone (without PRP) was used. Radiographs were recorded at 1 month, 2 months, 4 months and 6 months intervals after surgery to evaluate the defect bone fill in both groups. Results: The post-operative successive radiographs in the test group showed a significantly greater regeneration of bone in the height of bony defects with application of PRP to bone graft as compared to the control group. In the test group, by the first post operative month, about 58% of the defect was filled, which gradually increased in each month and showed about 94% of defect-fill by 6 months. In the control group, similar observation revealed only 31%of defect-fill by the first post-operative month and a 47% defect-fill at 6 months. Conclusion: The addition of PRP to bone graft appeared to enhance bone regeneration considerably. The combination of PRP and bone graft might have a potential for routine clinical use for regeneration of cystic bony defects in children.

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Bone Formation in the Maxillary Sinus by Using Platelet-rich Plasma: An Experimental Study in Sheep

Journal of Oral Implantology ◽

10.1563/0-692.1 ◽

2005 ◽

Vol 31 (1) ◽

pp. 2-17 ◽

Cited By ~ 46

Author(s):

Edgar Grageda ◽

Jaime L. Lozada ◽

Phillip J. Boyne ◽

Nicholas Caplanis ◽

Paul J. McMillan

Keyword(s):

Platelet Count ◽

Bone Regeneration ◽

Maxillary Sinus ◽

Platelet Rich Plasma ◽

Test Group ◽

Bone Allograft ◽

Autogenous Bone ◽

Control Group ◽

Freeze Dried ◽

Log Ratio

Abstract Recently, platelet-rich plasma (PRP) has been proven to be an effective regeneration adjunct when combined with autogenous bone in the reconstruction of mandibular defects. However, little is known about the effect of PRP when combined with a bone allograft in the maxillary sinus. The purpose of this study was to quantitatively evaluate the ability of PRP to enhance bone regeneration in the maxillary sinus of sheep when combined with demineralized freeze-dried bone allograft (DFDBA) and cortical cancellous freeze-dried bone allograft (CCFDBA). Ten sheep were selected for bilateral sinus augmentation DFDBA + CCFDBA + PRP (test) and DFDBA + CCFDBA (control). Five were sacrificed at 3 months and the other 5 at 6 months. Hematology tests were performed for platelet count, and histology slides were obtained for histomorphometric analysis taking 2 measures of interest: total area (square millimeters) and percentage of bone fill. Student t tests showed no significant difference between test and control groups for total area (P > .25) and percentage of bone fill (P > .80) at either 3 or 6 months. The control group showed no statistical difference for total area (P < .095) and percentage of bone fill (P < .60) between 3- and 6-month healing times. The test group, however, showed a significant increase in total area (P < .025) but not in percentage of bone fill (P < .40) for the 2 healing periods. When the treatments were compared for interactions within the animal model, no clear tendency was evident for the test group to perform in relation to the control group regarding total area (r = .766, P < .01). A moderate tendency existed between the percentages of bone filled (r = .824, P < .005). Platelet-rich plasma showed higher platelet count than did the whole blood (2 to 5 times). However, no correlation was found between the log ratio and the bone measures. Within the limitations of this study, PRP failed to enhance or accelerate bone regeneration in the maxillary sinus of sheep when combined with bone allograft.

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Application of BMP-2 for bone graft in Dentistry

RSBO ◽

10.21726/rsbo.v12i1.176 ◽

2016 ◽

Vol 12 (1) ◽

pp. 88

Author(s):

Lídia Audrey Rocha Valadas Marques ◽

Edvan Alves da Costa Júnior ◽

Mara Assef Leitão Lotif ◽

Edilson Martins Rodrigues Neto ◽

Francisco Filipe Carvalho da Silva ◽

...

Keyword(s):

Life Expectancy ◽

Bone Graft ◽

Bone Regeneration ◽

Tooth Loss ◽

Clinical Use ◽

Increase Life Expectancy ◽

Bone Replacement ◽

Morphogenetic Protein ◽

The Face ◽

Global Increase

Introduction: The global increase life expectancy and the resulting tooth loss has required searching for new rehabilitation alternatives in Dentistry. Biomaterials can be defined as any material that acts replacing a lost bone defect and its function. In Dentistry, many studies have aimed to improve bone regeneration through the use of BMPs for bone replacement. Objective: To review the literature on the use and clinical viability of human morphogenetic protein for the jaws reconstruction. Material and methods: The following databases were searched: Pubmed, Bireme, Lilacs, and Scielo and 30 articles published between 1965 and 2013 were found using the following descriptors: “dental implants”, “maxillary sinus”, and “biocompatible materials”. Results: Several studies demonstrate the biological advantages of rhBMP-2 on bone regeneration of the jaws. In recent years, morphogenetic protein has presented a large clinical use. Conclusion: Despite being a high-cost biomaterial, rhBMP-2 is a viable and very effective alternative for reconstruction of defects of the face.

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Enhanced regeneration of bone defects using sintered porous Ti6Al4V scaffolds incorporated with mesenchymal stem cells and platelet-rich plasma

RSC Advances ◽

10.1039/d0ra10215f ◽

2021 ◽

Vol 11 (9) ◽

pp. 5128-5138

Author(s):

Ji Li ◽

Ketao Wang ◽

Xiaowei Bai ◽

Qi Wang ◽

Ningyu Lv ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Regeneration ◽

Platelet Rich Plasma ◽

Bone Defects ◽

Regeneration Of Bone

Porous Ti6AI4V scaffolds incorporated with MSC and PRP are more effective in enhancing the bone regeneration.

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The effect of mesenchymal stem cells, demineralized bone graft and platelet-rich plasma on osteogenesis in rat tibia defects

International Dental Research ◽

10.5577/intdentres.2021.vol11.suppl1.8 ◽

2021 ◽

Vol 11 (Suppl. 1) ◽

pp. 47-55

Author(s):

Zozan Erdoğmuş ◽

Belgin Gülsün

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Graft ◽

Platelet Rich Plasma ◽

Female Rats ◽

Control Group ◽

Osteoblastic Activity ◽

Group I ◽

Rat Tibia ◽

Demineralized Bone

Aim: Deformities of the jaw and face are often caused by infection, inflammation, and cystic and neoplastic pathological conditions. Defects with various aetiologies should be repaired promptly using the most appropriate approach to reconstruct the anatomical form. To treat defects, bone grafts with various combinations have been used. In particular, combinations including cellular products to enhance osteogenic properties have been implemented. In this study, we aimed to investigate the effects of different materials and cells on bone defects by using mesenchymal stem cells (MSCs), which are thought to have a positive effect on healing, demineralized bone graft (DMB) and platelet-rich plasma (PRP). Methodology: We used 55 female rats weighing between 200-250 g, four of which were used to obtain platelet-rich plasma. The remaining animals were divided into five groups. Group I (n = 6) was the operative control group, Group II (n = 24) was given DMB, Group III (n = 24) was given DMB+PRP, Group IV (n = 24) was given MSC+DBG and Group V (n = 24) was given DMB+PRP+MSC applied to rat tibial defects (10 mm x 3 mm x 2 mm). Results: Statistically significant differences were observed in bone osteoblastic activity in tibia defects among the groups (p<0.05). Conclusion: Bone regeneration was significantly improved in groups where MSCs were used in combination with DMB and PRP. How to cite this article: Erdoğmuş Z, Gülsün B. The effect of mesenchymal stem cells, demıneralızed bone graft and platelet-rıch plasma on osteogenesıs ın rat tıbıa defects. Int Dent Res 2021;11(Suppl.1):47-55. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.8 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model

Materials ◽

10.3390/ma12101613 ◽

2019 ◽

Vol 12 (10) ◽

pp. 1613

Author(s):

Eunhye Jang ◽

Ja-Youn Lee ◽

Eun-Young Lee ◽

Hyun Seok

Keyword(s):

Bone Formation ◽

Bone Graft ◽

Bone Regeneration ◽

Bone Morphogenetic Protein ◽

Human Bone ◽

Control Group ◽

New Bone Formation ◽

Morphogenetic Protein ◽

Regeneration Effect ◽

Recombinant Human Bone

The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP (p = 0.004) and control groups (p = 0.000) and higher TbTh than the control group (p = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group (p = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group (p = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.

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Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Materials ◽

10.3390/ma13010018 ◽

2019 ◽

Vol 13 (1) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Jungwon Lee ◽

Young-Jun Lim ◽

Bongju Kim ◽

Ki-Tae Koo ◽

Yong-Moo Lee

Keyword(s):

Bone Graft ◽

Double Layer ◽

Single Layer ◽

Test Group ◽

Control Group ◽

Collagen Membrane ◽

Ridge Preservation ◽

Graft Material ◽

Membrane Technique ◽

Layer Membrane

The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

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Periapical tissue repair of dog teeth after intracanal administration of capsaicin 0.075%

Brazilian Dental Science ◽

10.14295/bds.2015.v18i1.1070 ◽

2015 ◽

Vol 18 (1) ◽

pp. 88

Author(s):

Nair Narumi Orita Pavan ◽

Marcos Sergio Endo ◽

Marina Viudes Bruder ◽

Angelo José Pavan ◽

Eduardo Grossmann ◽

...

Keyword(s):

Inflammatory Response ◽

Calcium Hydroxide ◽

Tissue Repair ◽

Test Group ◽

Control Group ◽

Clinical Use ◽

Atypical Odontalgia ◽

Root Canals

Objective: The aim of this study was make a descriptively evaluate the repair of the periapical region of dog teeth. Material and Methods: Thirty-six root canals were used,eighteen were filled with 0.075% capsaicin and calcium hydroxide (test paste)and the rest eighteen root canals were filled with calcium hydroxide (control group). Histological analyzes were performed to classify inflammation as absent, mild, moderate or severe. The analyses also classified the presence or absence of repairat 7, 14 and 30 days.Results: The use of 0.075% capsaicin did not modify the inflammatory response and repairwhen compared to control group. Conclusion: Our data suggests that test group with 0.075% capsaicin as intracanal administration could be indicated for tissue repair; furthermore other experiments should be carried out to evaluate possible clinical use of capsaicin for atypical odontalgia.Keywords: Capsaicin; Repair; Inflammation; Periapical tissue.

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Microcomputed Tomography and Histological Study of Bone Regeneration Using Tooth Biomaterial with BMP-2 in Rabbit Calvarial Defects

Scanning ◽

10.1155/2021/6690221 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Puneet Wadhwa ◽

Jeong Hun Lee ◽

Bing Cheng Zhao ◽

HongXin Cai ◽

Jae-Suk Rim ◽

...

Keyword(s):

Bone Graft ◽

Bone Regeneration ◽

Histological Study ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

Control Group ◽

Block Type ◽

Graft Material ◽

Synthetic Bone ◽

Ct Analysis

Our study was aimed to analyze the osteoinductive effect of powdered and block type autogenous bone graft along with bone morphogenetic protein (BMP-2) as compared to synthetic bone graft. Three circular bicortical defects were made in the calvaria of each rabbit and randomly divided into three groups as follows: powdered tooth biomaterial+BMP-2, block tooth biomaterial+BMP-2, and control group: synthetic bone+BMP-2. The samples taken from these defects after 4 and 8 weeks were analyzed histologically along with micro CT analysis. In our study, both powered and block type tooth autogenous bone graft successfully stimulated mesenchymal cells leading to endochondral ossification and bone regeneration. We observed that the powered bone graft material which is acid insoluble especially is preferable as a carrier for BMP-2.

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A study on the use of henna plant (Lawsonia inermis Linn) for the treatment of fungal disease (Trichophypton verrucosum) in calves

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.25923 ◽

2021 ◽

Vol 71 (4) ◽

pp. 2483

Author(s):

H. HIZLI

Keyword(s):

Clinical Examination ◽

Treatment Process ◽

Fungal Disease ◽

Repeated Measurement ◽

Control Group ◽

Lawsonia Inermis ◽

Group Control ◽

Animal Material ◽

Holstein Calves ◽

Group Control Group

The aim of this study was to investigate the usability of henna plant (Lawsonia inermis Linn) in the treatment of dermatophytosis lesions (Trichophypton verrucosum) in cattle. The animal material of the trial consisted of 50 holstein calves between the ages of 4 and 6 months, who were found to have a dermatophytosis lesion on their face and neck in their clinical examination. The experiment was organized on a three-group repeated measurement trial plan. I. Group: Trichlorfon (Neguvon 75%, Bayer) ointment, II. Group: Henna applied, and III. Group: Control Group, no treatment, and 20 (10 females, 10 males), 20 (10 females, 10 males), and 10 (5 females, 5 males) totally 50 calves used, respectively. The research was continued for 14 days until the lesions were completely healed. I. and II. Groups were observed the best healing in the calves, respectively. In the III. Group without any treatment, there was no improvement and the lesions were enlarged. In addition, the effect of gender in the treatment process of dermatophytosis lesions was insignificant. As a result of this study, it is thought that henna plant can be used in the treatment of dermatophytosis.

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The Teratogenic Mechanism of Echinochrome as a Hypoglycemic Agent on Wistar Rats

The Open Biomarkers Journal ◽

10.2174/1875318301909010079 ◽

2019 ◽

Vol 9 (1) ◽

pp. 79-83

Author(s):

Ayman S. Mohamed ◽

Eman Y. Salah EL Din ◽

Neveen A. Farag ◽

Abdel Rahman A. Tawfik

Keyword(s):

Wistar Rats ◽

Low Dose ◽

Body Weight Gain ◽

Control Group ◽

High Dose ◽

Teratogenic Effects ◽

Pregnant Rats ◽

Hypoglycemic Agent ◽

Group Control ◽

Group Control Group

Background: Echinochrome (Ech) is the active ingredient in the Histochrome drug, which possesses strong antioxidant, hypolipidemic and hypoglycemic activity. Objective: The present work aimed to characterize the malformations induced by moderate and high dose of Ech during pregnancy. Methods: In this study, eighteen (18) female pregnant rats were assigned into 3 groups (6 rats/ group); control group, low dose Ech (0.1 mg/kg) and high dose Ech (1 mg/kg). Results: The high dose of Ech caused a significant decrease in the number of embryos, uteri weight, body weight gain, placenta weight, and embryo weight and length. Also, the high dose led to a significant increase in serum AST, ALT, ALP, urea and uric acid of mothers. Conclusion: Our findings revealed the first teratogenic effects of high dose Ech. The teratogenic mechanism of Ech works through induction of the hypoglycemic condition in pregnant rats.

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