The effect of mesenchymal stem cells, demineralized bone graft and platelet-rich plasma on osteogenesis in rat tibia defects

Aim: Deformities of the jaw and face are often caused by infection, inflammation, and cystic and neoplastic pathological conditions. Defects with various aetiologies should be repaired promptly using the most appropriate approach to reconstruct the anatomical form. To treat defects, bone grafts with various combinations have been used. In particular, combinations including cellular products to enhance osteogenic properties have been implemented. In this study, we aimed to investigate the effects of different materials and cells on bone defects by using mesenchymal stem cells (MSCs), which are thought to have a positive effect on healing, demineralized bone graft (DMB) and platelet-rich plasma (PRP). Methodology: We used 55 female rats weighing between 200-250 g, four of which were used to obtain platelet-rich plasma. The remaining animals were divided into five groups. Group I (n = 6) was the operative control group, Group II (n = 24) was given DMB, Group III (n = 24) was given DMB+PRP, Group IV (n = 24) was given MSC+DBG and Group V (n = 24) was given DMB+PRP+MSC applied to rat tibial defects (10 mm x 3 mm x 2 mm). Results: Statistically significant differences were observed in bone osteoblastic activity in tibia defects among the groups (p<0.05). Conclusion: Bone regeneration was significantly improved in groups where MSCs were used in combination with DMB and PRP. How to cite this article: Erdoğmuş Z, Gülsün B. The effect of mesenchymal stem cells, demıneralızed bone graft and platelet-rıch plasma on osteogenesıs ın rat tıbıa defects. Int Dent Res 2021;11(Suppl.1):47-55. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.8 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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Survival Analysis after Core Decompression in Association with Platelet-Rich Plasma, Mesenchymal Stem Cells, and Synthetic Bone Graft in Patients with Osteonecrosis of the Femoral Head

Joints ◽

10.1055/s-0038-1626740 ◽

2018 ◽

Vol 06 (01) ◽

pp. 016-022 ◽

Cited By ~ 5

Author(s):

Riccardo D'Ambrosi ◽

Elena Biancardi ◽

Giulia Massari ◽

Vincenza Ragone ◽

Renato Facchini

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Femoral Head ◽

Bone Graft ◽

Platelet Rich Plasma ◽

Core Decompression ◽

Stage I ◽

Synthetic Bone ◽

Synthetic Bone Graft ◽

Kaplan Meier

Purpose The aim of this study was to report the rate of survivorship in patients with osteonecrosis of the femoral head treated with core decompression in association with mesenchymal stem cells (MSCs) implantation, platelet-rich plasma (PRP) injection, and synthetic bone graft. Methods We evaluated 24 hips in 16 patients, according to Ficat classification, treated by core decompression, injection of PRP and MSCs, and backfilling of the core tract with synthetic bone graft. Survivorship was estimated using Kaplan–Meier curves. Results The survivorship of core decompression in association with the procedure is 50% at 75 months of follow-up. The survival rate was 80% for patients in early stage and 28.6% for patients in advanced stage at 75 months. When we compared Kaplan–Meier survival curves of patients in stage III + IV and patients in stage I + II, we noticed that the survival functions are statistically different (p < 0.05, log-rank test), particularly in stage I + II where we had a greater surviving core decompression, in comparison to patients in stage III + IV. Conclusion This technique is safe and good preliminary results were obtained in patients with early stages of the disease with no reported complications. Level of Evidence Level IV, therapeutic case series.

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Comparative Study on the Effect of Adipose tissue derived Mesenchymal Stem Cells versus Metformin on Age-related Structural Changes of the Cerebellum in Albino Rat

QJM ◽

10.1093/qjmed/hcab099.003 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

M. M Doaa Karem ◽

Soheir K Ahmed ◽

Hanan A Saleh ◽

Ghada Galal Hamam

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Purkinje Cells ◽

Structural Changes ◽

Female Rats ◽

Group Iii ◽

Age Related ◽

Group I ◽

Group Ii

Abstract Introduction Aging is a normal physiological process that affects all organs in the body including the cerebellum. Metformin is an anti-diabetic drug that is used in some age-related diseases. Regenerative medicine using adipose tissue derived mesenchymal stem cells (ADMSCs) is an emerging promising strategy. Aim to compare between the role of ADMSCs and metformin on the age-related structural changes of the cerebellum in female albino rats. Materials and methods Fifty-five female rats of different ages (4, 12 and 24 months) were included in this study. They were divided into three groups according to their ages: Group I (Adult rats), Group II (Old rats) and Group III (Senile rats). Group II and Group III were subdivided into three subgroups, Subgroup a: rats were left without treatment, Subgroup b: rats were given a single dose of 1X106 ADMSCs via tail vein. Subgroup c: Rats received300 mg/kg metformin/day orally. Rats were sacrificed after four weeks. The cerebellum was collected and processed for H&E, Toluidine blue and immuno-histochemical reaction using glial fibrillary acidic protein (GFAP). Results Histological examination of the cerebellum of the subgroups IIa and IIIa revealed age-related structural changes in comparison to group I. Purkinje cells appeared distorted with irregular outline. Some Purkinje cells were seen shrunken while others appeared ballooned. Focal loss of Purkinje cells was also noticed. Granular layer contained small widely separated granule cells. GFAP reaction revealed an apparent decrease in number of astrocytes and their processes. The structural changes were more obvious in subgroup IIIa. In ADMSCs treated subgroups (IIbandIIIb); more noticeable improvement of these changes was noticed compared to the corresponding metformin treated subgroups (IIc and IIIc). Conclusion ADMSCs was more effective than metformin in preventing some age-related structural changes of the cerebellum.

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Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

Applied Sciences ◽

10.3390/app112210900 ◽

2021 ◽

Vol 11 (22) ◽

pp. 10900

Author(s):

Abdulsamet Emet ◽

Erdi Ozdemir ◽

Duygu Uckan Cetinkaya ◽

Emine Kilic ◽

Ramin Hashemihesar ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Bone Defects ◽

Control Group ◽

Albino Rats ◽

Group 5 ◽

Group 2 ◽

Wistar Albino Rats ◽

Critical Bone Defects

The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (n = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (p = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (p = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.

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CONTENT OF PRIMARY AND SECONDARY LIPID PEROXIDATION PRODUCTS IN SUBCELLULAR FRACTIONS OF CARDIOMYOCYTES DURING MYOCARDIAL INFARCTION IN RATS IN AN EXPERIMENT AND THEIR CORRECTION BY TRANSPLANTATION OF MESENCHYMAL STEM CELLS

Archiv Euromedica ◽

10.35630/2199-885x/2021/11/4.exsu ◽

2021 ◽

Vol 11 (4) ◽

pp. 89-92

Author(s):

Vyacheslav Mykhaylichenko ◽

Andrey Pilipchuk ◽

Dmitry Parshin ◽

Yuri Kostyamin

Keyword(s):

Myocardial Infarction ◽

Lipid Peroxidation ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Subcellular Fractions ◽

Control Group ◽

Diene Conjugates ◽

Mesenchymal Stem Cell Transplantation ◽

Lipid Peroxidation Products ◽

Group I

Experimental modeling of myocardial infarction in rats was carried out by ligation of the anterior intergastric artery after the first division. There were 3 groups of 20 animals each: control group I — to verify normal parameters, group II — a model of myocardial infarction, and group III — animals which, after modeling myocardial infarction, underwent transplantation of mesenchymal stem cells. The level of lipid peroxidation products — diene conjugates and malondialdehyde — was studied by spectrophotometry. Comparison of the content and their ratio in the cytoplasm and mitochondria of myocardiocytes was carried out. It turned out that transplantation of mesenchymal stem cells significantly levels the activation of lipid peroxidation processes in subcellular fractions of cardiomyocytes, which is accompanied by a decrease in the primary and secondary products of oxidative stress. The ratio of malondialdehyde to diene conjugates both in the cytoplasm and in the mitochondria of cardiomyocytes after transplantation returned to control values. This indicates the normalization of physiological processes with underlying ischemic heart damage. The results indicate the cytoprotective effect of mesenchymal stem cell transplantation and the preservation of a larger number of cell pools, compared with the control group of animals that did not receive any treatment.

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Histomorphology of Pancreatic Islet in Physiological Aging Female Rats Post Intravenous Human Wharton’s Jelly Mesenchymal Stem Cell Injection

Journal of Stem Cell Research and Tissue Engineering ◽

10.20473/jscrte.v3i1.16324 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Wining Astini

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Treatment Group ◽

Pancreatic Islet ◽

Aging Treatment ◽

Wharton’S Jelly ◽

Female Rats ◽

Control Group ◽

Female Rat ◽

Wharton's Jelly

The increasing population of aged people will have the important role in the life, but the function of their bodies will decrease because of aging. Aging will increase the risk of degenerative disease, one of example is diabetes. The disease is related to the aging in the pancreatic organ which progressively declines by age. The aimed of the experiment was to determine the effect of human wharton’s jelly mesenchymal stem cells by injecting intravenously in aging female rats. This study used 3 young female rats (3 months) and 6 aging female rats (24 months). The experiment consisted of three groups. The young control group (A), the aging control group (B) that received NaCl (0.9%) 0,4 mL, the aging treatment group (C) received 1 x 106 cells/kg of human wharton’s jelly mesenchymal stem cells 0,4 mL. The aging control and the aging treatment group were injected 4 times with the interval in 3 months. The end of the experiment (12 months), the rats were anesthetized and sacrificed. The pancreatic tissues were collected to examine the pancreatic islets by histology studies. Changes of the pancreatic islet in control and treated groups were examined using hematoxylin and eosin staining. These findings conclude that injecting human wharton’s jelly mesenchymal stem cell increase the diameter and total pancreatic islet in the treatment group. In other side, the cell population of pancreatic islet also have significant differences (P<0.05) in treated physiological aging female rat groups than control aging female rat group.

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Mesenchymal stem cells reduce both inflammation and mortality in chronic cerebral murine toxoplasmosis

QJM ◽

10.1093/qjmed/hcaa060 ◽

2020 ◽

Vol 113 (Supplement_1) ◽

Author(s):

L M Elhosseiny ◽

A F Badawy ◽

A M Elashkar ◽

F A Abuzahra ◽

N M Abdelsamee ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Mortality Rate ◽

Infection Control ◽

Conventional Treatment ◽

Histopathological Examination ◽

Treated Group ◽

Combination Group ◽

Control Group ◽

Group I

Abstract Bone marrow-derived mesenchymal stem cells (BM-MSCs) are self-renewing, clonal precursors of non-haematopoietic tissues, with anti-inflammatory and anti-apoptotic effect. This study aimed to evaluate the effect of BM-MSCs on chronic toxoplasmosis. BM-MSCs were isolated from 6-wk-old BALB/c donor male mice, then grown and propagated in culture until cell count was 5–8x106/ml. Female Swiss albino mice were divided into five groups: Group I (infected mice injected with BM-MSCs); Group II (infected mice treated with both BM-MSCs and conventional treatment); Group III (infected mice conventionally treated with Spiramycin-Metronidazole combination); Group IV (infection control group in which mice were infected with Me49 strain of Toxoplasma gondii) and Group V (non-infected mice injected with BM-MSCs). Histopathological examination of brain tissue and survival rate were assessed in each group. Compared to the infection control group and conventionally treated group, the infected mice injected with BM-MSCs showed less tissue damage, mild inflammatory changes in brain sections and low mortality rate. The group treated with both MSCs and conventional treatment showed unexpected sever inflammation and the highest mortality rate.

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Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

10.21203/rs.3.rs-806042/v1 ◽

2021 ◽

Author(s):

Abdulsamet Emet ◽

Erdi Ozdemir ◽

Duygu Uckan Cetinkaya ◽

Emine Kilic ◽

Ali Celalettin Sinan Yuruker ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Bone Defects ◽

Control Group ◽

Albino Rats ◽

Group 5 ◽

Group 2 ◽

New Research ◽

Critical Bone Defects

Abstract Background: High-cost and the long time needed for the treatment of critical-sized bone defects is still major clinical concern in orthopedic surgery, therefore new research continues to identify more cost and time-effective methods. In this study, we aimed to investigate the effect of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical- sized bone defects. Materlials and methods: Wistar albino rats (n=30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. To increase healing, no scaffold, decellularized omentum, omentum with with PRP and omentum mesenchymal stem cells was used in group 1 (control group) , 2, 3 and 4 respectively. In addition omentum with a combination of mesenchymal stem cells and PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among groups. Results: After use of decellularized omentum scaffold, vitality of new cells maintained, new bone formation occurred. When compared to control group, radiological healing was statistically significantly better in omentum and omentum with PRP-treated groups (p=0.047). Furthermore histological healing was better in the omentum and omentum with PRP-treated groups than both control and omentum with mesenchymal stem cell-treated groups according to Salkeld Scoring System (p=0.001).Conclusion: The use of decellularized omentum scaffold is suitable in the healing of critical bone defects.

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Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin?

Biochemistry and Cell Biology ◽

10.1139/bcb-2016-0224 ◽

2017 ◽

Vol 95 (5) ◽

pp. 537-548 ◽

Cited By ~ 9

Author(s):

Hanan Hosni Ahmed ◽

Laila Ahmed Rashed ◽

Sohair Mahfouz ◽

Rania Elsayed Hussein ◽

Marwa Alkaffas ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Transforming Growth Factor ◽

Burn Injury ◽

Platelet Rich Plasma ◽

In Vivo Studies ◽

Control Group ◽

Tnf Α

Our aim was to study the effect of platelet-rich plasma (PRP) on the proliferation of bone-marrow-derived mesenchymal stem cells (BM-MSCs) and to investigate their roles in the healing of experimental burn injury and the possible mechanism of action. Our work was divided into in-vitro and in-vivo studies. The in-vitro study included untreated MSCs and MSCs treated with PRP. Levels of TGF-β and cell proliferation were assessed. In the in-vivo study, 72 rats were distributed equally among 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP, and burn with MSCs pretreated with PRP. On the 7th and 20th day after injury, the serum levels of transforming growth factor beta (TGF-β) and tumor necrosis factor alpha (TNF-α), as well as interleukin-10 (IL-10) levels in skin tissue were measured by ELISA; histopathology and gene expression of MMP-1, TIMP-2, Ang-1, Ang-2, and vimentin by real-time PCR were performed in all groups. In vitro: proliferation of MSCs and TGF-β increased in the PRP-treated group compared with the control group. In vivo: Ang-1, Ang-2, and vimentin were upregulated, whereas MMP-1 and TIMP-2 were downregulated. TGF-β and IL-10 were increased, whereas TNF-α was decreased in all treated groups with more significance in MSCs and PRP on day 20. Histopathology of burn skin was improved in all treated groups, particularly in MSCs pretreated with PRP 20 days post-burn.

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Therapeutic effect of mesenchymal stem cells on histopathological, immunohistochemical, and molecular analysis in second-grade burn model

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02365-y ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Doaa Ramadan I. Abdel-Gawad ◽

Walaa A. Moselhy ◽

Rasha Rashad Ahmed ◽

Hessah Mohammed Al-Muzafar ◽

Kamal Adel Amin ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Transforming Growth Factor ◽

Quantitative Polymerase Chain Reaction ◽

Normal Skin ◽

Transforming Growth Factor Β ◽

Control Group ◽

Albino Rats ◽

Group I

Abstract Background and aim Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns and examine the effect of BM-MSCs during a short and long period of therapy. Material and methods Ninety albino rats were divided into three groups: group I (control); group II (burn model), the animals were exposed to the preheated aluminum bar at 100°C for 15 s; and group III (the burned animals subcutaneously injected with BM-MSCs (2×106 cells/ ml)); they were clinically observed and sacrificed at different short and long time intervals, and skin samples were collected for histopathological and immunohistochemical examination and analysis of different wound healing mediators via quantitative polymerase chain reaction (qPCR). Results Subcutaneous injection of BM-MSCs resulted in the decrease of the wound contraction rate; the wound having a pinpoint appearance and regular arrangement of the epidermal layer with thin stratum corneum; decrease in the area percentages of ADAMs10 expression; significant downregulation of transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), metalloproteinase-9 (MMP-9), and microRNA-21; and marked upregulation of heat shock protein-90α (HSP-90α) especially in late stages. Conclusion BM-MSCs exhibited a powerful healing property through regulating the mediators of wound healing and restoring the normal skin structures, reducing the scar formation and the wound size.

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Effect of Transplantation of Bone Marrow Derived Mesenchymal Stem Cells and Platelets Rich Plasma on Experimental Model of Radiation Induced Oral Mucosal Injury in Albino Rats

International Journal of Dentistry ◽

10.1155/2017/8634540 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Basma Elsaadany ◽

Samar El Kholy ◽

Dalia El Rouby ◽

Laila Rashed ◽

Tarek Shouman

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Synergetic Effect ◽

Neck Region ◽

Control Group ◽

Albino Rats ◽

Histological Structure ◽

Apoptotic Activity

Normal tissue damage following radiotherapy is still a major problem in cancer treatment. Therefore, the current work aimed at exploring the possible role of systemically injected bone marrow derived mesenchymal stem cells (BM-MSCs) and/or locally injected platelet rich plasma (PRP) in ameliorating the side effects of ionizing radiation on the rat’s tongue. Twelve rats served as control group (N) and 48 rats received a single radiation dose of 13 Gy to the head and neck region; then, they were equally divided into 4 experimental groups: irradiated only (C), irradiated + MSCs (S), irradiated + (PRP) (P), and combined group (PS). Animal scarification occurred in 3 and 7 days after radiation. Then, tongues were dissected and examined histologically and for expression of bcl-2 by RT-PCR. Histological examination of the treated groups (S), (P), and (PS) revealed an obvious improvement in the histological structure of the tongue, compared to group (C), in addition to upregulated expression of bcl-2, indicating decreased apoptotic activity.Conclusion. BM-MSCs and PRP have shown positive effect in minimizing the epithelial atrophy of normal oral mucosa after regional radiotherapy, which was emphasized by decreasing apoptotic activity in these tissues. Nevertheless, combined use of BM-MSCs and PRP did not reveal the assumed synergetic effect in oral tissue protection.

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