scholarly journals Animal models of chronic pain. In vivo experiments

2021 ◽  
Vol 14 (3) ◽  
pp. 121-129
Author(s):  
V. V. Khinovker ◽  
E. V. Khinovker
Keyword(s):  
Chronic Pain ◽  
Population Aging ◽  
In Vitro Models ◽  
Medical Problems ◽  
Rat Models ◽  
In Vivo Experiments ◽  
The One ◽  
Different Levels

As a result of population aging and increasing of comorbide patients procent, chronic pain nowadays is the one of the most important medical problems and its treatment becoming one of progressive and popular researching topics. First part of acute or chronic pain research is a model that meets the specified criteria. First model of cronic pain sciatic nerve damaging was founded and proven more than 30 years ago. Nowadays there is a lot of in vivo and in vitro models that matched with different pathologies and also mechanisms of chronic nociceptive and neuropathic pain on different levels have been learned. In this article we review the most effective and often used rat models of chronic pain its mechanisms and assessment methods. The information based on the most citated articles for 10 years.

scholarly journals How to Achieve High-Quality Oocytes? The Key Role of Myo-Inositol and Melatonin

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Salvatore Giovanni Vitale ◽  
Paola Rossetti ◽  
Francesco Corrado ◽  
Agnese Maria Chiara Rapisarda ◽  
Sandro La Vignera ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Oocyte Quality ◽  
Fertilization Rate ◽  
In Vitro Models ◽  
The One ◽  
High Level

Assisted reproductive technologies (ART) have experienced growing interest from infertile patients seeking to become pregnant. The quality of oocytes plays a pivotal role in determining ART outcomes. Although many authors have studied how supplementation therapy may affect this important parameter for both in vivo and in vitro models, data are not yet robust enough to support firm conclusions. Regarding this last point, in this review our objective has been to evaluate the state of the art regarding supplementation with melatonin and myo-inositol in order to improve oocyte quality during ART. On the one hand, the antioxidant effect of melatonin is well known as being useful during ovulation and oocyte incubation, two occasions with a high level of oxidative stress. On the other hand, myo-inositol is important in cellular structure and in cellular signaling pathways. Our analysis suggests that the use of these two molecules may significantly improve the quality of oocytes and the quality of embryos: melatonin seems to raise the fertilization rate, and myo-inositol improves the pregnancy rate, although all published studies do not fully agree with these conclusions. However, previous studies have demonstrated that cotreatment improves these results compared with melatonin alone or myo-inositol alone. We recommend that further studies be performed in order to confirm these positive outcomes in routine ART treatment.

scholarly journals A Four-Inflow Construction to Ensure Thermal Stability and Uniformity during Hyperthermic Intraperitoneal Chemotherapy (HIPEC) in Rats

Cancers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3516
Author(s):  
Daan R. Löke ◽  
Roxan F. C. P. A. Helderman ◽  
Jan Sijbrands ◽  
Hans M. Rodermond ◽  
Pieter J. Tanis ◽  
...  
Keyword(s):  
Intraperitoneal Chemotherapy ◽  
Computer Aided Design ◽  
Hyperthermic Intraperitoneal Chemotherapy ◽  
In Vivo Experiments ◽  
The One ◽  
Aided Design ◽  
The Impact ◽  
Phosphate Buffered Saline

Background: Hyperthermic intraperitoneal chemotherapy (HIPEC) after cytoreductive surgery (CRS) is used for treating peritoneal metastases of various origins. Present HIPEC protocols have rarely been validated for relevant parameters such as optimal agent, duration and perfusate temperature. In vitro experiments are not completely representative of clinical circumstances. Therefore, a good preclinical in vivo HIPEC model is needed in which temperature distributions can be well-controlled and are stable throughout treatments. Methods: We designed a setup able to generate and maintain a homogeneous flow during a 90-min HIPEC procedure using our in-house developed treatment planning tools and computer aided design (CAD) techniques. Twelve rats were treated with heated phosphate-buffered saline (PBS) using two catheter setups (one vs. four- inflows) and extensive thermometry. Simulated and measured thermal distribution and core temperatures were evaluated for the different setups. Results: Overall, the four-inflow resulted in more stable and more homogeneous thermal distributions than the one-inflow, with lower standard deviations (0.79 °C vs. 1.41 °C at the outflow, respectively) and less thermal losses. The average thermal loss was 0.4 °C lower for rats treated with the four-inflow setup. Rat core temperatures were kept stable using occasional tail cooling, and rarely exceeded 39 °C. Conclusion: Increasing the number of inflow catheters from one to four resulted in increased flow and temperature homogeneity and stability. Tail cooling is an adequate technique to prevent rats from overheating during 90-min treatments. This validated design can improve accuracy in future in vivo experiments investigating the impact of relevant parameters on the efficacy of different HIPEC protocols.

scholarly journals Investigation of Equine In Vivo and In Vitro Derived Metabolites of the Selective Androgen Receptor Modulator (SARM) ACP-105 for Improved Doping Control

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 85
Author(s):  
Malin Nilsson Broberg ◽  
Heather Knych ◽  
Ulf Bondesson ◽  
Curt Pettersson ◽  
Scott Stanley ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
High Performance ◽  
Metabolite Profile ◽  
In Vitro Models ◽  
Doping Control ◽  
Cunninghamella Elegans ◽  
In Vivo Experiments ◽  
Receptor Modulator

Selective Androgen Receptor Modulators (SARMs) have anabolic properties but less adverse effects than anabolic androgenic steroids. They are prohibited in both equine and human sports and there have been several cases of SARMs findings reported over the last few years. The aim of this study was to investigate the metabolite profile of the SARM ACP-105 (2-chloro-4-[(3-endo)-3-hydroxy-3-methyl-8-azabicyclo[3.2.1]oct-8-yl]-3-methylbenzonitrile) in order to find analytical targets for doping control. Oral administration of ACP-105 was performed in horses, where blood and urine samples were collected over a time period of 96 h. The in vivo samples were compared with five in vitro incubation models encompassing Cunninghamella elegans, microsomes and S9 fractions of both human and equine origin. The analyses were performed using ultra-high performance liquid chromatography coupled to high resolution Q ExactiveTM OrbitrapTM mass spectrometry (UHPLC-HRMS). A total of 21 metabolites were tentatively identified from the in vivo experiments, of which several novel glucuronides were detected in plasma and urine. In hydrolyzed urine, hydroxylated metabolites dominated. The in vitro models yielded several biotransformation products, including a number of monohydroxylated metabolites matching the in vivo results. The suggested analytical target for equine doping control in plasma is a dihydroxylated metabolite with a net loss of two hydrogens. In urine, the suggested targets are two monohydroxylated metabolites after hydrolysis with β-glucuronidase, selected both due to prolongation of the detection time and the availability of reference material from the in vitro models.

Beryllium Metal Solubility in the Lung, Comparison of Metal and Hot-pressed Forms by in vivo and in vitro Dissolution Bioassays

1987 ◽  
Vol 6 (3) ◽  
pp. 233-240 ◽  
Author(s):  
S. André ◽  
H. Métivier ◽  
G. Lantenois ◽  
M. Boyer ◽  
D. Nolibé ◽  
...  
Keyword(s):  
Occupational Safety ◽  
In Vitro Models ◽  
In Vitro Dissolution ◽  
In Vivo Model ◽  
Health Administration ◽  
In Vivo Experiments ◽  
Safety And Health ◽  
Dissolution Model

The solubility of two industrial forms of beryllium, i.e. particles of metal powder and particles of hot-pressed beryllium, was investigated using in vivo and in vitro models. In the in vivo model, baboons and rats were used and were injected via the trachea with amounts of beryllium equivalent to 100, 500 and 1000 fold the maximum permissible concentration (MPC) recommended by the US Occupational Safety and Health Administration. In vivo experiments showed that in both species the daily beryllium solubility rates were about 5 x 10-6 for metal particles and that in rats the daily beryllium solubility rate was about 5 x 10-5 for the hot-pressed particles. During the 10 months of the experiment with baboons, urinary excretion of beryllium was proportional to the amount administered. With regard to results for the in vitro models, the outcome of the acellular dissolution test using a serum simulant was not consistent with the in vivo results, though a cellular model using cultured macrophages showed the same trends in the dissolution rates for the two forms of beryllium as those observed in vivo. This result suggests that a cellular rather than an acellular dissolution model would be better at predicting solubility of beryllium compounds in the lungs.

scholarly journals The PsbS protein and low pH are necessary and sufficient to induce quenching in the light-harvesting complex of plants LHCII

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lauren Nicol ◽  
Roberta Croce
Keyword(s):  
Low Ph ◽  
In Vivo Studies ◽  
Photochemical Quenching ◽  
Photosynthetic Membrane ◽  
Light Harvesting Complex ◽  
In Vivo Experiments ◽  
The One ◽  
Necessary And Sufficient

AbstractPhotosynthesis is tightly regulated in order to withstand dynamic light environments. Under high light intensities, a mechanism known as non-photochemical quenching (NPQ) dissipates excess excitation energy, protecting the photosynthetic machinery from damage. An obstacle that lies in the way of understanding the molecular mechanism of NPQ is the large gap between in vitro and in vivo studies. On the one hand, the complexity of the photosynthetic membrane makes it challenging to obtain molecular information from in vivo experiments. On the other hand, a suitable in vitro system for the study of quenching is not available. Here we have developed a minimal NPQ system using proteoliposomes. With this, we demonstrate that the combination of low pH and PsbS is both necessary and sufficient to induce quenching in LHCII, the main antenna complex of plants. This proteoliposome system can be further exploited to gain more insight into how PsbS and other factors (e.g. zeaxanthin) influence the quenching mechanism observed in LHCII.

Nuclear receptor coregulators

2003 ◽  
Vol 75 (11-12) ◽  
pp. 1665-1669 ◽  
Author(s):  
N. J. McKenna ◽  
B. W. O'Malley
Keyword(s):  
Ubiquitin Ligase ◽  
Rna Processing ◽  
In Vivo Experiments ◽  
Nuclear Receptor Coregulators ◽  
Context Specific ◽  
Enzyme Functions ◽  
Different Levels ◽  
Coactivator Complex

It has been postulated that nuclear receptors (NRs) regulate transcription via interactions with chromatin and the basal transcription machinery at the promoters of genes. Coregulators (coactivators or corepressors) are important in mediating these interactions and thereby modulating positive or negative receptor activity. A large number of putative coactivators have been isolated, several of which will be reviewed with respect to certain "criteria" initially proposed for coactivators. We will discuss, with reference to in vitro and in vivo experiments, the main steps in initiation that are influenced by coactivators: (1) initiation (e.g., SRC-1 family, CBP); (2) repetitive transcription (e.g., TRAPs/DRIPs); (3) RNA processing (PGC-1, etc); and (4) termination/turnover (E6-AP, etc). A variety of enzyme functions have been implicated in the coactivator complex including acetylase, methylase, ubiquitin ligase, kinase, and phosphatase activities. Moreover, coactivators and corepressors appear to exist in the steady-state cell as a series of multiprotein complexes referred to collectively as the "coregulatorsome". Different subcomplexes within the coregulatorsome may have different levels of preference for individual receptors or promoters, likely contributing to context-specific functions of NRs in target tissues.

scholarly journals Looking for In Vitro Models for Retinal Diseases

2021 ◽  
Vol 22 (19) ◽  
pp. 10334
Author(s):  
Margherita Alfonsetti ◽  
Vanessa Castelli ◽  
Michele d’Angelo ◽  
Elisabetta Benedetti ◽  
Marcello Allegretti ◽  
...  
Keyword(s):  
Visual Function ◽  
Retinal Disease ◽  
3D Models ◽  
In Vitro Models ◽  
Retinal Diseases ◽  
In Vivo Models ◽  
In Vivo Experiments ◽  
Toxicological Studies

Retina is a layered structure of the eye, composed of different cellular components working together to produce a complex visual output. Because of its important role in visual function, retinal pathologies commonly represent the main causes of visual injury and blindness in the industrialized world. It is important to develop in vitro models of retinal diseases to use them in first screenings before translating in in vivo experiments and clinics. For this reason, it is important to develop bidimensional (2D) models that are more suitable for drug screening and toxicological studies and tridimensional (3D) models, which can replicate physiological conditions, for investigating pathological mechanisms leading to visual loss. This review provides an overview of the most common retinal diseases, relating to in vivo models, with a specific focus on alternative 2D and 3D in vitro models that can replicate the different cellular and matrix components of retinal layers, as well as injury insults that induce retinal disease and loss of the visual function.

ANTITUMOR EFFECT OF COLLOIDAL SILVER BISILICATE IN EXPERIMENTAL IN VITRO AND IN VIVO STUDIES

2019 ◽  
Vol 65 (5) ◽  
pp. 760-765
Author(s):  
Margarita Tyndyk ◽  
Irina Popovich ◽  
A. Malek ◽  
R. Samsonov ◽  
N. Germanov ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Tumor Growth ◽  
Tumor Cells ◽  
Human Tumor ◽  
Significant Inhibition ◽  
In Vivo Studies ◽  
Ehrlich Carcinoma ◽  
In Vivo Experiments

The paper presents the results of the research on the antitumor activity of a new drug - atomic clusters of silver (ACS), the colloidal solution of nanostructured silver bisilicate Ag6Si2O7 with particles size of 1-2 nm in deionized water. In vitro studies to evaluate the effect of various ACS concentrations in human tumor cells cultures (breast cancer, colon carcinoma and prostate cancer) were conducted. The highest antitumor activity of ACS was observed in dilutions from 2.7 mg/l to 5.1 mg/l, resulting in the death of tumor cells in all studied cell cultures. In vivo experiments on transplanted Ehrlich carcinoma model in mice consuming 0.75 mg/kg ACS with drinking water revealed significant inhibition of tumor growth since the 14th day of experiment (maximally by 52% on the 28th day, p < 0.05) in comparison with control. Subcutaneous injections of 2.5 mg/kg ACS inhibited Ehrlich's tumor growth on the 7th and 10th days of the experiment (p < 0.05) as compared to control.

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