STUDY OF THE ACUTE TOXICITY OF AQUADES-NUK PRODUCTS 5

2021 ◽  
Author(s):  
Oleg P. Pugach ◽  
Alexander M. Lunegov ◽  
Irina V. Lunegova
Keyword(s):  
Acute Toxicity ◽  
Animal Feed ◽  
Raw Materials ◽  
Animal Husbandry ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Source Of Infection ◽  
White Rats ◽  
Quality Of Products ◽  
Susceptible Organism

In the system of veterinary and sanitary measures, disinfection occupies one of the important places, contributing to ensuring the welfare of animal husbandry against infectious diseases, increasing the productivity of animals, poultry and the sanitary quality of products, raw materials and animal feed. The main purpose of disinfection is to break the epizootic chain by influencing its most important link - the factor of transmission of the causative agent of the disease from the source of infection to the susceptible organism. Based on the foregoing, we were assigned the goal of studying the characteristics of the new domestic disinfectant AQUAdez-NUK 5, and to achieve this goal, one of the tasks was to study acute toxicity. In order to study the acute toxicity of AQUAdes-NUK 5 when administered orally, experiments were carried out on male Wister white rats weighing 220-230 g. at doses of 2000, 3000, 4000 and 5500 mg / kg. For the experiments, the rats were divided into groups of 10 animals. 10 animals were used to study each dosage for each disinfectant. One group of ten animals served as a control, which was injected with 0.9% sodium chloride solution. The disinfectant was injected in pure form. After administration, the laboratory animals were monitored for two weeks. The experiment took into account the death of animals and the clinical picture of intoxication. In the course of laboratory studies, we determined that AQUAdez-NUK 5 belongs to the III hazard class in accordance with GOST 12.1.007-76 (moderately hazardous compounds), as well as the presence of a weak irritant effect, and therefore we took appropriate safety measures during work with this disinfectant.

TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

1970 ◽  
pp. 67-69
Author(s):  
E. K. Rakhmatullin ◽  
O. D. Sklyarov
Keyword(s):  
Drug Toxicity ◽  
Probit Analysis ◽  
Laboratory Animals ◽  
Control Group ◽  
Hazard Class ◽  
Water Ingestion ◽  
White Rats ◽  
Poorly Soluble ◽  
And Behavior ◽  
Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

scholarly journals Acute Oral Toxicity of the Supramolecular Complex Based on Albendazole and Triclabendazole (Altric-Extra) in Laboratory Outbred Mice and Rats

2020 ◽  
Vol 14 (1) ◽  
pp. 64-69
Author(s):  
Ekaterina V. Lagereva ◽  
Vladislav E. Abramov
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
The Body ◽  
Male Rats ◽  
Laboratory Animals ◽  
Oral Toxicity ◽  
Hazard Class ◽  
Russian Research Institute ◽  
Starch Gel ◽  
Outbred Mice

The purpose of the research is to evaluate the acute toxicity of Altric-Extra when introduced into the stomach to mice and rats. Materials and methods. The studies were conducted in the vivarium of the All-Russian Research Institute of Fundamental and Applied Parasitology of Animals and Plants. The acute toxicity of Altric-Extra was determined on 20 white outbred male mice weighing 19.3–23.3 g, 10 animals in a group and on 30 white outbred male rats weighing 150–196 g, 6 animals in a group. Altric-Extra was administered to mice of the experimental group once into the stomach in the form of a suspension in a dose of 5,986 mg/kg at the rate of 0.2 ml/10 g of body weight. Altric-Extra rats were also administered once into the stomach in the form of a suspension at the rate of 2.0 ml/100 g body weight. As a carrier in the preparation of the suspension, 1% starch gel was used. The experimental rats of groups 1, 2, 3 and 4 were given Altric-Extra at doses of 4,580.2 mg/kg, 3,846.2; 3,088.8 and 1,577.9 mg/ kg respectively. Mice and rats of the control groups were administered once with 1% starch gel. For 14 days, the behavior and condition of the animals was monitored. The body weight of the experimental animals was measured on the 1st, 3rd, 7th, 9th and 14th days of the experiment. Results and discussion. Medium lethal doses of LD50 have been established for oral administration to laboratory animals. For mice, the LD50 was more than 5 986 mg/kg, i.e., according to the generally accepted hygienic classification, Altrick-Extra belongs to hazard class 4 (low-hazard substances). On rats, the LD50 was 3 103.1±48.5 mg/kg (2,354.6÷3,851.5 mg/kg). Therefore, Altrik-Extra belongs to hazard class 3 (substances are moderately hazardous).

scholarly journals Toxicological characteristics of anticoccidial preparation Decvycox on laboratory animals

2020 ◽  
pp. 51-57
Author(s):  
Lyudmila Mikhaylovna Kashkovskaya ◽  
Andrey Vladimirovich Balyshev ◽  
Vladimir Aleksandrovich Orobets ◽  
Ivan Aleksandrovich Fedorov
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Prevention And Treatment ◽  
The Common

The common practice in the prevention and treatment of eimeriosis in chickens is the application of anticoccidial preparations. The parameters of acute toxicity of the anticoccidial preparations Decvycox in laboratory animals were studied. It was found out that the LD50 of Decvycox with oral administration to mice and rats exceeds doses of 6579 and 7222 mg/kg of animal weight, respectively. Thus, according to the generally accepted hygienic classification, Decvycox belongs to hazard class 4 - low-hazard substances.

scholarly journals Acute Toxicity of Hydrogel Polyhexamethylene Guanidine Hydrochloride

2020 ◽  
Vol 5 (4) ◽  
pp. 103-107
Author(s):  
S. N. Lebedeva ◽  
O. S. Ochirov ◽  
M. N. Grigoryeva ◽  
S. D. Zhamsaranova ◽  
S. A. Stelmakh ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Guanidine Hydrochloride ◽  
Lethal Dose ◽  
The Body ◽  
Laboratory Animals ◽  
Intragastric Administration ◽  
Test Substance ◽  
Hazard Class ◽  
Polyhexamethylene Guanidine ◽  
Medicinal Substances

Background. Previously, we have shown that the polyhexamethylene guanidine hydrochloride hydrogel exhibits a pronounced wound healing. At the same time, no studies of the toxic effect of the hydrogel on animals have been conducted. Aim of the research. In the framework of this work, the acute toxicity of the hydrogel polyhexamethylene guanidine hydrochloride was studied in laboratory animals with intragastric administration. Materials and methods. The polyhexamethylene guanidine hydrochloride hydrogel was obtained by crosslinking the amino end groups with formaldehyde. An acute toxicity study was carried out (P 1.2.3156-13, GOST 32644-2014 and the Guidelines for conducting preclinical studies of drugs) in an experiment on outbred mice with a single addition of the test substance in different doses (1000, 3000, 5000, 8000 mg/kg) with fixing indicators (appearance, behavior, condition of the body hair coat, water and food consumption, excretion, body weight and its growth) during 14 days. After the animals were withdrawn from the experiment, autopsy, macroscopic evaluation and weighing of the internal organs were performed. The results showed that with the introduction of the test substance into the animal organism, death during the observation period (14 days) did not occur. It was not possible to determine the semi-lethal dose for the test compound. Conclusion. The conducted studies allow us to conclude that this substance is practically non-toxic and can be classified as hazard class V. Further research will be directed to the formation of hydrogel compositions with medicinal substances.

scholarly journals Study of the parameters of acute toxicity of the drug “Devimectin 1 %” with a single subcutaneous injection in white rats

2020 ◽  
Vol 22 (100) ◽  
pp. 28-31
Author(s):  
Yu. R. Hunchak ◽  
B. V. Gutyj ◽  
R. M. Sachuk ◽  
Ya. S. Stravsky
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Subcutaneous Administration ◽  
Female Rats ◽  
Laboratory Animals ◽  
Control Group ◽  
Main Experiment ◽  
Sterile Saline ◽  
White Rats ◽  
Therapeutic Properties

In the study of the drug for injectable use – “Devimectin 1 %”, together with the confirmation of therapeutic properties, it is necessary to determine the LD50 obtained in the study of acute toxicity. The aim of the work was to study the acute toxicity of “Devimectin 1 %” in white rats by injection. To fulfill this goal on the principle of analogues was formed control and three experimental groups of 4 animals each (n = 4). The drug was administered in doses of 5000.0; 10000.0; 20000.0 mg/kg body weight in absolute weight of the drug once subcutaneously in the withers. The control animals were injected subcutaneously with sterile saline 1.0 cm3. After taking into account the results of the previous experiment in the main experiment, 7 experimental groups were formed, whose rats were injected subcutaneously with “Devimectin 1 %” in doses of 5000.0; 7500.0; 10000.0; 12500.0; 15000.0; 17500.0 and 20000.0 mg/kg body weight, as well as a control group to which animals were injected with sterile saline with a volume of 1.0 cm3. There were 6 animals in each group (n = 6). It was found that for the administration of the drug at a dose of 5000 mg / kg body weight, no animal died, for 10000.0 and 20000.0 mg/kg body weight, respectively, one and 4 animals died. Death occurred for 2–6 days depending on the administered dose. In the main experiment with subcutaneous administration of “Devimectin 1 %” at a dose of 5000.0 mg/kg body weight during the 14-day period of the study, no animal died; for the introduction of the drug at a dose of 7500.0 mg/kg killed one animal; for 10000.0 – 2; for 12500.0 and 15000.0 – 3 rats; for 17500.0 – 5 rats and for the introduction of the drug at a dose of 20000.0 mg/kg body weight, all experimental animals died. The death of laboratory animals occurred for 2–6 days depending on the administered dose. According to the results of studies, it was found that the LD50 of the drug “Devimectin 1 %” under the conditions of its single subcutaneous administration to female rats is 12881.20 ± 1390.54 mg/kg, LD10 – 5978.43 mg/kg, LD16 – 7495.68 mg/kg, LD84 – 18266.73 mg/kg, LD90 – 19783.98 mg/kg, LD100 – 20959.49 mg/kg body weight, respectively. Therefore, the drug “Devimectin 1%” when administered subcutaneously can be classified as toxicity class VI – substances relatively harmless (LD50subcut> 4500,0 mg/kg). Further studies will be the next step in pre-registration trials to examine the subacute toxicity of “Devimectin 1 %”.

scholarly journals Aplikasi Mesin Pencacah Pakan Ternak Serbaguna Sebagai Upaya Mengurangi Pengolahan Pakan Ternak Secara Konvensional

2019 ◽  
Vol 3 (1) ◽  
pp. 43 ◽  
Author(s):  
Nur Ihda Farikhatin Nisa ◽  
Achmad Aminudin ◽  
Yoga Akhdiat Fahrudi
Keyword(s):  
Animal Feed ◽  
Raw Materials ◽  
Animal Husbandry ◽  
Skills Training ◽  
Raw Material ◽  
Powder Feed ◽  
Feed Production ◽  
Self Sufficiency ◽  
Cutting Knife ◽  
Soybean Cake

The provision of animal feed in the form of forages or soybeans made by goat farmers in general is still traditional. Drajat Village is one of the villages where the goat breeders still use conventional methods in processing their feed. The aspect needed in small to medium scale farms is the speed of feed production time in the form of powder feed that is ready for use. To fulfill this aspect, we made goat feed chopping machines in the implementation of this Community Service Program (PKM). The animal feed chopping machine is designed with various stages including observation, consultation, science and technology diffusion, training and socialization of the use of tools. The components that are made include the engine frame, cutting knife and trajectory of entering the raw material (soybean cake). Whereas for components purchased are pulleys, V-belts, drive motors, shafts, casings and bearing bearings. From these components the assembly and manufacturing process will be carried out using tool kit equipment. The aim of this program is to provide skills training to groups of goat farmers in processing feed sources by doing mechanical processing through modernization of livestock with multipurpose animal feed counting machines, reducing the level of difficulty in animal feed search especially in the dry season and providing insight into entrepreneurship in the field of animal husbandry utilize raw materials to do the grinding process and then can be marketed to farmers in various regions. The results of this program are the achievement of a group of goat farmers who are able to produce or process animal feed independently in powder form. When animal feed ingredients are abundant, they make powder feed and store it as food self-sufficiency during the dry season.ABSTRAK Pemberian pakan ternak berupa hijauan ataupun onggok kedelai yang dilakukan oleh peternak kambing pada umumnya masih bersifat tradisional. Desa Drajat adalah salah satu  desa dimana para peternak kambingnya masih  menggunakan cara konvensional dalam pengolahan pakannya. Aspek yang dibutuhkan dalam peternakan skala kecil hingga menengah adalah kecepatan waktu produksi pakan dalam bentuk pakan serbuk yang siap digunakan. Untuk memenuhi aspek tersebut, kami membuat mesin pencacah pakan ternak kambing dalam pelaksanaan program Pengabdian Kepada Masyarakat (PKM) ini. Mesin pencacah pakan ternak dirancang dengan berbagai tahapan meliputi observasi, konsultasi, difusi iptek, pelatihan dan sosialisasi penggunaan alat. Adapun komponen yang dibuat meliputi rangka mesin, pisau potong dan lintasan masuk bahan baku (onggok kedelai). Sedangkan untuk komponen yang dibeli yaitu pulley, V-belt, motor penggerak, poros, casing dan bantalan bearing. Dari komponen-komponen tersebut akan dilakukan proses perakitan dan pembuatan dengan menggunakan peralatan tool kit. Tujuan dari program ini adalah untuk memberikan pelatihan keterampilan kepada kelompok peternak kambing dalam mengolah sumber pakan dengan melakukan penggolahan secara mekanis melalui modernisasi peternakan dengan mesin pencacah pakan ternak serbaguna, mengurangi tingkat kesulitan dalam pencarian pakan ternak terutama dimusim kemarau serta memberikan wawasan berwirausaha dalam bidang peternakan dengan memanfaatkan bahan baku untuk dilakukan proses penggilingan dan selanjutnya bisa dipasarkan ke peternak di berbagai daerah. Hasil dari program ini yaitu tercapainya kelompok peternak kambing yang mampu memproduksi atau mengolah secara mandiri pakan ternak dalam bentuk serbuk. Pada saat bahan baku pakan ternak melimpah, mereka membuat pakan serbuk dan menyimpannya sebagai swasembada pangan pada saat musim kemarau tiba.Kata kunci : mesin pencacah; onggok kedelai; pakan ternak; kambing

scholarly journals STUDY OF ACUTE TOXICITY OF NOVEL ANTITUMOR PREPARATION BASED ON DERIVATIVE OF INDOLOCARBAZOLE - LCS-1208

2015 ◽  
Vol 14 (4) ◽  
pp. 59-64 ◽  
Author(s):  
A. A. Nikolina ◽  
N. Ju. Kul'bachevskaja ◽  
O. I. Konjaeva ◽  
V. A. Chalej ◽  
N. P. Ermakova ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Intraperitoneal Administration ◽  
Laboratory Animals ◽  
Antitumor Preparation ◽  
White Rats ◽  
Hybrid Mice

The research has been devoted to the study of acute toxicity of preparation based on derivative of indolocarba- zole - LCS-1208 in mice of both sexes and in rats of both sexes at intravenous and intraperitoneal administration of the drug. In the experiments the laboratory animals - outbred white rats and hybrid mice (C57Bl/6JxDBA2)Fl (B6D2F1) have been used. On the results of study has been obtained the calculated toxic doses of novel preparation based on derivative of indolocarbazole at intraperitoneal administration of the drug in mice of both sexes.

scholarly journals STUDY OF THE CUMULATIVE PROPERTIES OF «INDEZ» ON LABORATORY WHITE RATES

2020 ◽  
Vol 21 (1) ◽  
pp. 98-104
Author(s):  
I. Ja. Kotsyumbas ◽  
O. M. Brezvyn ◽  
Y. A. Ivashkiv ◽  
H. V. Rudyk ◽  
Ju. V. Muzika
Keyword(s):  
Body Weight ◽  
Total Dose ◽  
Animal Husbandry ◽  
Bactericidal Effect ◽  
Laboratory Animals ◽  
Control Group ◽  
White Rats ◽  
Weight One ◽  
Iron Sulphate ◽  
Spleen Mass

The article presents the results of the study of «Indez» disinfectant. An effective disinfectant for use in animal husbandry should be easy to use, be non-toxic, have broad bactericidal spectrum, be non-carcinogenic, have non-addictive micro-flora and provide a permanent bactericidal effect in the presence of animals, while sanitizing the air environment. «Indez» disinfectant is a small, amorphous grey powder with a specific odour, well sprayed. It is composed of triiodomethane (iodoform), zinc oxide, iron sulphate (II) (iron sulphate), copper sulphate, silicon dioxide, zeolite, active essential oils, a complex of surfactants and pH regulators, auxiliaries. This preparation can be used in the presence of animals; the disinfectant effect is based on the spectrum of antimicrobial action of its constituents. In the experiment of the study of the cumulative properties of «Indez» 80 white rats weighing 180-200 ± 10 g, tested doses of 1/5, 1/10, 1/20, from DL50 were used. To the animals of the experimental group, the suspension of the preparation was administered orally: in the first 4 days - 1/5 of DL50, then 1/10 and 1/20. Animals of the control group were administered saline at a dose of 0.5 ml. Observations on rats were carried out for 22 days. Depending on the dose of the drug, the cumulation coefficient. As a result of the study of the cumulative properties of the drug it is established that the introduction of a total dose of «Indez» 56,8 cm3 per 1 kg body weight does not cause death of white rats. When conducting a total dose of 63,4 cm3 / kg body weight one animal died, representing 5 %. Further administration of the drug lethality was on the 18-th day (total dose 83,13 cm3 / kg) – 15 %, on the 19-th day (total dose 93,0 cm3 / kg) – 20 % and the 20-th day (total dose 102,87 cm3 / kg) – 45 %. With an increase of 9,873 1,5 times (1,8095 cm3 / kg) on the 21-st day, the mortality rate was 80 %, and on the 22-nd day of the studies 90 % of laboratory animals were killed, the total dose was 1032,49 cm3 / kg. Under the study of the cumulative properties of «Indez» disinfectant, it was found that the cumulation coefficient in rats is 2,2 units. This, in turn, indicates that the test agent has moderately pronounced properties for cumulation. In this case, white rats inhibit the hematopoietic function of the bone marrow and reduce the body’s defences, as evidenced by a probable decrease in leukocyte count and a slight decrease in haemoglobin, lymphocyte count, spleen mass factor, and increased segmentation.

Inheritance and correlation of productive traits in wool-and-meat producing sheep of different genotypes

2020 ◽  
pp. 12-20
Author(s):  
B. Traisov ◽  
Yu. Yuldashbaev ◽  
N. Kulmakova ◽  
K. Esengaliev ◽  
D. Smagulov
Keyword(s):  
Raw Materials ◽  
Live Weight ◽  
Animal Husbandry ◽  
The West ◽  
Sheep Breeding ◽  
Genetic Potential ◽  
Coeffi Cients ◽  
Productive Traits ◽  
West Kazakhstan ◽  
Quality Of Products

The effectiveness and competitiveness of sheep breeding directly depends on increasing the productivity of animals and improving the quality of products obtained from them, and this has been achieving by targeted breeding using the genetic potential of sheep breeds. The solution to this problem can be implemented through the rational use of breed resources of domestic and imported sheep breeding. Sheep breeding in Kazakhstan is the oldest branch of animal husbandry, which plays a major role in providing the national economy with specifi c types of raw materials and food. Sheep produce the largest amount of various products: wool for diff erent purposes, mutton, fat raw materials, sheep’s milk, sheep skin-fur-leather raw materials, lamb pelts. The whole of Kazakhstan and the West Kazakhstan region are characterized by a wide variety of natural, climatic and economic factors that have a specifi c infl uence on the development of sheep breeding in certain regions. In the West Kazakhstan region breeding work is carried out in a herd of Akzhaik wool-and-meat producing sheep to improve individual productive indicators by using the genetic potential of the gene pool of diff erent semifi ne-wool breeds, so breeding and improvement in order to increase the productivity of Akzhaik meat-wool sheep with double productivity is relevant and has great economic signifi cance. The inheritance and correlation of productive traits in Akzhaik wool-and-meat producing sheep obtained from diff erent mating options have been studied in the paper. It has been established that the inheritance of the main economically useful traits is additive in nature in an intermediate type with minor deviations towards one of the parent forms. The coeffi cients of heritability of live weight, clip, length and fi ber fi neness of wool are within the average values. The highest correlation coeffi cients (0,65–0,70) have been found in all groups between the length and fi ber fi neness of wool.

Study of Utilization of Agricultural Waste as Environmental Issue in Romania

2017 ◽  
Vol 68 (3) ◽  
pp. 570-575 ◽  
Author(s):  
George Ungureanu ◽  
Gabriela Ignat ◽  
Catalin Razvan Vintu ◽  
Constantin Daniel Diaconu ◽  
Ioan Gabriel Sandu
Keyword(s):  
Animal Feed ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Animal Husbandry ◽  
Agricultural Wastes ◽  
Waste Products ◽  
Common Solution ◽  
Oat Straw ◽  
Crop Waste ◽  
Agriculture Wastes

Agriculture and animal husbandry produce significant quantity of solid or liquid residues and waste products. Unfortunately, some of these wastes are not dealt with properly and are causing considerable damage to the environment. Agricultural wastes (AW) in Romania amount range from 7600 thousand tons a year of which only 1400 thousand tons as animal feed and 1100 thousand tons as organic manure are being utilized. These crop waste results after harvesting in the farm of leaves stem which are characterized as coarse plant by-products and big size, chemically low in protein and fat contents, or, like other country (example: Netherlands) avoid waste as much as possible, recover the valuable raw materials from any waste that is created, try to generate energy by incinerating the residual waste, and only then dump what is left. The focus of the research paper is to investigate the importance of agriculture wastes that becomes very obvious and aggregated after the harvest crops. The most common solution is the utilizations of agricultural waste for compositing, as animal fodder, most often as a source of energy, food production, by growing mushroom on agricultural wastes such as oat straw as a substrate. This means the conversion of wastes to economic, nutritional human food. Growing vegetables on oat straw compacted bales in areas where soil disease and salinity are constrains. The implementation of most of the solutions to agricultural waste management does not meet the basic elements of sustainability like environmental protection and social progression, technical and technological improvement as well as economic improvements.

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