scholarly journals STUDY OF ACUTE TOXICITY OF NOVEL ANTITUMOR PREPARATION BASED ON DERIVATIVE OF INDOLOCARBAZOLE - LCS-1208

2015 ◽  
Vol 14 (4) ◽  
pp. 59-64 ◽  
Author(s):  
A. A. Nikolina ◽  
N. Ju. Kul'bachevskaja ◽  
O. I. Konjaeva ◽  
V. A. Chalej ◽  
N. P. Ermakova ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Intraperitoneal Administration ◽  
Laboratory Animals ◽  
Antitumor Preparation ◽  
White Rats ◽  
Hybrid Mice

The research has been devoted to the study of acute toxicity of preparation based on derivative of indolocarba- zole - LCS-1208 in mice of both sexes and in rats of both sexes at intravenous and intraperitoneal administration of the drug. In the experiments the laboratory animals - outbred white rats and hybrid mice (C57Bl/6JxDBA2)Fl (B6D2F1) have been used. On the results of study has been obtained the calculated toxic doses of novel preparation based on derivative of indolocarbazole at intraperitoneal administration of the drug in mice of both sexes.

scholarly journals Study on Acute Toxicity of Amiodarone New Complexes With Cyclodextrin

2021 ◽  
Vol 12 ◽  
Author(s):  
Cristina Mihaela Ghiciuc ◽  
Maytham Razaq Shleghm ◽  
Cornelia Vasile ◽  
Gladiola Tantaru ◽  
Andreea Creteanu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Intraperitoneal Administration ◽  
Control Sample ◽  
Controlled Drug Release ◽  
Laboratory Animals ◽  
High Permeability ◽  
Cyclodextrin Complex ◽  
The Matrix ◽  
Low Solubility ◽  
Powdered Form

Amiodarone low solubility and high permeability is the limiting step for its bioavailability, therefore new formulations are needed to improve the solubility of amiodarone either to increase its oral bioavailability or to reduce its toxic effects. Complexation of amiodarone with cyclodextrin results in improved dissolution rate, solubility, and allows for a more controlled drug release. We characterized the acute toxicity of a new amiodarone 2-hydroxypropyl-β-cyclodextrin complex (AMD/HP-β-CD) as powdered form and as a matrix based on Kollidon® and chitosan, administered intraperitoneally in laboratory animals. There were developed two formulations of matrix: one containing only pure AMD as a control sample (Fc) and one containing the inclusion complex with the optimal solubility (F). AMD was equitoxic with HP-β-CD after intraperitoneal administration (289.4 mg/kg for AMD and 298.3 mg/kg for AMD/HP-β-CD), with corresponding histopathological changes. The matrix based formulations presented higher LD50 values for acute toxicity, of 347.5 mg/kg for Fc and 455.6 mg/kg for F10, conducting to the idea of a safer administration because KOL and CHT matrix modified the solubility and controlled the AMD release. The LD50 is 1.5 higher for AMD/HP-β-CD included in a KOL and CHT based matrix compared to the pure AMD, administered intraperitoneally.

STUDY OF THE ACUTE TOXICITY OF AQUADES-NUK PRODUCTS 5

2021 ◽  
Author(s):  
Oleg P. Pugach ◽  
Alexander M. Lunegov ◽  
Irina V. Lunegova
Keyword(s):  
Acute Toxicity ◽  
Animal Feed ◽  
Raw Materials ◽  
Animal Husbandry ◽  
Laboratory Animals ◽  
Hazard Class ◽  
Source Of Infection ◽  
White Rats ◽  
Quality Of Products ◽  
Susceptible Organism

In the system of veterinary and sanitary measures, disinfection occupies one of the important places, contributing to ensuring the welfare of animal husbandry against infectious diseases, increasing the productivity of animals, poultry and the sanitary quality of products, raw materials and animal feed. The main purpose of disinfection is to break the epizootic chain by influencing its most important link - the factor of transmission of the causative agent of the disease from the source of infection to the susceptible organism. Based on the foregoing, we were assigned the goal of studying the characteristics of the new domestic disinfectant AQUAdez-NUK 5, and to achieve this goal, one of the tasks was to study acute toxicity. In order to study the acute toxicity of AQUAdes-NUK 5 when administered orally, experiments were carried out on male Wister white rats weighing 220-230 g. at doses of 2000, 3000, 4000 and 5500 mg / kg. For the experiments, the rats were divided into groups of 10 animals. 10 animals were used to study each dosage for each disinfectant. One group of ten animals served as a control, which was injected with 0.9% sodium chloride solution. The disinfectant was injected in pure form. After administration, the laboratory animals were monitored for two weeks. The experiment took into account the death of animals and the clinical picture of intoxication. In the course of laboratory studies, we determined that AQUAdez-NUK 5 belongs to the III hazard class in accordance with GOST 12.1.007-76 (moderately hazardous compounds), as well as the presence of a weak irritant effect, and therefore we took appropriate safety measures during work with this disinfectant.

scholarly journals Study of the parameters of acute toxicity of the drug “Devimectin 1 %” with a single subcutaneous injection in white rats

2020 ◽  
Vol 22 (100) ◽  
pp. 28-31
Author(s):  
Yu. R. Hunchak ◽  
B. V. Gutyj ◽  
R. M. Sachuk ◽  
Ya. S. Stravsky
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Subcutaneous Administration ◽  
Female Rats ◽  
Laboratory Animals ◽  
Control Group ◽  
Main Experiment ◽  
Sterile Saline ◽  
White Rats ◽  
Therapeutic Properties

In the study of the drug for injectable use – “Devimectin 1 %”, together with the confirmation of therapeutic properties, it is necessary to determine the LD50 obtained in the study of acute toxicity. The aim of the work was to study the acute toxicity of “Devimectin 1 %” in white rats by injection. To fulfill this goal on the principle of analogues was formed control and three experimental groups of 4 animals each (n = 4). The drug was administered in doses of 5000.0; 10000.0; 20000.0 mg/kg body weight in absolute weight of the drug once subcutaneously in the withers. The control animals were injected subcutaneously with sterile saline 1.0 cm3. After taking into account the results of the previous experiment in the main experiment, 7 experimental groups were formed, whose rats were injected subcutaneously with “Devimectin 1 %” in doses of 5000.0; 7500.0; 10000.0; 12500.0; 15000.0; 17500.0 and 20000.0 mg/kg body weight, as well as a control group to which animals were injected with sterile saline with a volume of 1.0 cm3. There were 6 animals in each group (n = 6). It was found that for the administration of the drug at a dose of 5000 mg / kg body weight, no animal died, for 10000.0 and 20000.0 mg/kg body weight, respectively, one and 4 animals died. Death occurred for 2–6 days depending on the administered dose. In the main experiment with subcutaneous administration of “Devimectin 1 %” at a dose of 5000.0 mg/kg body weight during the 14-day period of the study, no animal died; for the introduction of the drug at a dose of 7500.0 mg/kg killed one animal; for 10000.0 – 2; for 12500.0 and 15000.0 – 3 rats; for 17500.0 – 5 rats and for the introduction of the drug at a dose of 20000.0 mg/kg body weight, all experimental animals died. The death of laboratory animals occurred for 2–6 days depending on the administered dose. According to the results of studies, it was found that the LD50 of the drug “Devimectin 1 %” under the conditions of its single subcutaneous administration to female rats is 12881.20 ± 1390.54 mg/kg, LD10 – 5978.43 mg/kg, LD16 – 7495.68 mg/kg, LD84 – 18266.73 mg/kg, LD90 – 19783.98 mg/kg, LD100 – 20959.49 mg/kg body weight, respectively. Therefore, the drug “Devimectin 1%” when administered subcutaneously can be classified as toxicity class VI – substances relatively harmless (LD50subcut> 4500,0 mg/kg). Further studies will be the next step in pre-registration trials to examine the subacute toxicity of “Devimectin 1 %”.

scholarly journals ACUTE TOXICITY TEST OF LOW CALCIUM OXALATE PORANG (Amorphophallus mueleri BLUME) FLOUR

2021 ◽  
Vol 52 (1) ◽  
pp. 218-231
Author(s):  
I. Donowarti ◽  
S. B. Widjanarko ◽  
Y. Yunianta ◽  
P. Pudjiastuti
Keyword(s):  
Acute Toxicity ◽  
Calcium Oxalate ◽  
Toxicity Test ◽  
Cell Damage ◽  
Female Rats ◽  
Laboratory Animals ◽  
Acute Toxicity Test ◽  
Food Ingredient ◽  
White Rats ◽  
Significant Difference

A field experiment Porang (Amorphophallus mueleri Blume) has the potential to be developed as a functional food ingredient because it contains high levels of glucomannan. Research on the acute toxicity test of macerated porang flour has been carried out. The results of research showed a toxic effect which was characterized by high SGOT and sodium levels. The purpose of this study was to find out the safety level of consuming porang flour with lowered calcium oxalate content. This research was an experimental study designed in one directional-pattern Completely Randomized Design using 5 treatments of porang flour administration with doses of 0; 5; 50; 500; 5000 and 15000 mg/kgbw and 6 repetitions for 60 days using Wistar-strain white rats (Rattus norvegicus) as laboratory animals. The results showed that during the treatment, the administration up to a dose of 500 mg/kgbw did not give a significant difference to all observed variables. The administration of 5000 and 15000 mg/kgbw gave a significant difference on the changes in body weight, the addition of the amount of water drunk, the levels of Calcium, Potassium and Sodium in the blood, SGOT and SGPT values, and observation on necrotic cells in the kidneys. The administration of the highest porang flour dosage, namely 15000 mg/kgbw did not cause any rat mortality and did not cause any real cell damage to the liver, but caused hyperactive behavior in female rats.

scholarly journals Determination of acute toxicity of the ‘Bondarmin’ disinfectant when administered intraperitoneally to laboratory animals

2020 ◽  
Vol 6 (4) ◽  
pp. 25-28
Author(s):  
A. O. Bondarchuk ◽  
A. P. Paliy ◽  
A. P. Palii ◽  
A. P. Aksonov
Keyword(s):  
Acute Toxicity ◽  
Toxic Effect ◽  
Intraperitoneal Administration ◽  
Toxicity Assessment ◽  
Laboratory Animals ◽  
Toxic Substances ◽  
Toxicological Studies ◽  
National University ◽  
Acute Toxic Effect ◽  
Scientific Laboratory

The article presents the results of the study of the acute toxic effect of the innovative disinfectant ‘Bondarmin’ (active substance — potassium peroxomonosulfate) on laboratory animals (mice, rats) are presented. Many scientific works of scientists in recent years have been devoted to the study of the toxicity of various disinfectants both in our country and abroad. However, today there are many topical issues regarding the toxicity and safety of some antimicrobials. Our work aimed to study the toxic effect on the laboratory animals and to establish the acute toxicity (LD50) of the developed disinfectant ‘Bondarmin’ when administered intraperitoneally. Experiments were carried out in the Laboratory of Pharmacology and Toxicology of the National University of Pharmacy (Kharkiv) and in the Educational and Scientific Laboratory of Genetic and Molecular Research Methods named after P. I. Verbitskiy in the Kharkiv State Zooveterinary Academy. Acute toxicity assessment (LD50) was carried out with intraperitoneal administration of the designed disinfectant to laboratory animals (mice, rats). The toxic effect of the newly developed disinfectant ‘Bondarmin’ for the intraperitoneal method of administration to laboratory animals (mice, rats) has been determined. For the intraperitoneal administration of the ‘Bondarmin’ disinfectant, the LD50 by Prozorovskiy method is 316.85 ± 19.26 mg/kg for mice, and 279.33 ± 19.80 mg/kg for rats. The disinfectant belongs to the IV toxicity class (low toxic substances). The results of toxicological studies allow us to recommend the use of ‘Bondarmin’ for disinfecting livestock facilities

Determination of acute toxicity of Tiacyclin solution for injection in laboratory animals

2019 ◽  
Vol 331 (8) ◽  
pp. 25-27
Author(s):  
N.Y. Morozov ◽  
◽  
S.I. Tchukina ◽  
E.I. Koveshnikova ◽  
◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Laboratory Animals

TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

1970 ◽  
pp. 67-69
Author(s):  
E. K. Rakhmatullin ◽  
O. D. Sklyarov
Keyword(s):  
Drug Toxicity ◽  
Probit Analysis ◽  
Laboratory Animals ◽  
Control Group ◽  
Hazard Class ◽  
Water Ingestion ◽  
White Rats ◽  
Poorly Soluble ◽  
And Behavior ◽  
Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

TOXIC EFFECTS ASSESSMENT OF NICKEL OXIDE NANOPARTICLES BY INHALATION

2018 ◽  
pp. 24-29 ◽  
Author(s):  
B.A. Katsnelson ◽  
M.P. Sutunkova ◽  
L.I. Privalova ◽  
S.N. Solovjeva ◽  
V.B. Gurvich ◽  
...  
Keyword(s):  
Nickel Oxide ◽  
Redox Balance ◽  
Systemic Toxicity ◽  
Laboratory Animals ◽  
Oxide Nanoparticles ◽  
Nickel Oxide Nanoparticles ◽  
White Rats ◽  
Liver And Kidney ◽  
Stimulation Of Erythropoiesis ◽  
Nio Nps

The article presents in an experiment obtained principal results based on repeated low-level inhalation exposures of laboratory animals (white rats, outbred) to nickel oxide nanoparticles with a diameter of (23 ± 5) nm, 4 hours a day, 5 times a week for up to 10 months in a «nose only» installation. It was shown that non-specific body reactions to the action of NiO NPs include: diverse manifestations of systemic toxicity with a particularly pronounced influence on liver and kidney function, redox balance, damage to some areas of brain tissue, associated with proven movement of the nanoparticles themselves from the nasal mucosa along the olfactory tract; some cytological signs of probable development for allergic syndrome; paradoxically low severity of pulmonary pathology by pneumoconiotic type explained by a small chronic delay of nanoparticles in the lungs; the genotoxic effect of the organismal level, even at those low levels of chronic exposure, at which systemic toxicity is rather poorly. Along with that, NiO NPs also induce phase-stimulation of erythropoiesis, which is relatively specific for the toxic nickel effects.

scholarly journals Effects of Pfaffia glomerata (Spreng) pedersen aqueous extract on healing acetic acid-induced ulcers

2008 ◽  
Vol 51 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Cristina Setim Freitas ◽  
Cristiane Hatsuko Baggio ◽  
Samanta Luiza Araújo ◽  
Maria Consuelo Andrade Marques
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Healing Process ◽  
Intraperitoneal Administration ◽  
Oral Route ◽  
Chronic Gastric Ulcer ◽  
Pfaffia Glomerata ◽  
Tissue Healing

The present study was carried out to evaluate the acute toxicity and the effect of the aqueous extract of the roots from Pfaffia glomerata (Spreng) Pedersen (Amaranthaceae) (AEP) on the prevention of acetic acid-induced ulcer and on the healing process of previously induced ulcers. The acute toxicity was evaluated in Swiss mice after oral administration of a single dose and the chronic gastric ulcer was induced with local application of acetic acid. The results showed that the LD50 of the extract was 684.6 mg.kg-1 for the intraperitoneal administration and higher than 10 mg.kg-1by the oral route. The administration of the AEP did not prevent ulcers formation. However, the AEP increased of the healing process of previously induced ulcers. The results suggest that AEP chronically administered promote an increase of tissue healing, after the damage induced by acetic acid and the extract seemed to be destituted of toxic effects in the mice by the oral route.

scholarly journals Determination of acute toxicity parameters of the drug ‘MEGASTOP for dogs’ on white rats and mice

2020 ◽  
Vol 6 (2) ◽  
pp. 20-26
Author(s):  
O. L. Orobchenko ◽  
M. Ye. Romanko ◽  
M. O. Yaroshenko ◽  
I. O. Gerilovych ◽  
N. A. Zhukova ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Clinical Symptoms ◽  
Liver Volume ◽  
Male Rats ◽  
Toxic Substances ◽  
Main Experiment ◽  
Polyethylene Glycol 400 ◽  
White Rats ◽  
Rats And Mice

The experiments were performed on 58 males of nonlinear white rats 3–4 months old and weighing 180–200 g and 64 females of nonlinear white mice 2.5–3 months old and weighing 18–22 g. In the main experiment on rats, six experimental groups were formed, the animals of which were injected intragastrically with the drug ‘MEGASTOP for dogs’ (by absolute weight) in doses of 1,000.0, 2,000.0, 3,000.0, 4,000.0, 5,000.0, and 6,000.0 mg/kg body weight; in the main experiment on mice, seven experimental groups were formed, the animals of which were administered the drug in doses of 100.0, 500.0, 1,000.0, 1,500.0, 2,000.0, 2,500.0, and 3,000.0 mg/kg body weight. Control rats and mice were injected with 2.0 cm3 and 0.2 cm3 of polyethylene glycol-400, respectively. Clinical symptoms of poisoning with the drug ‘MEGASTOP for dogs’ of white rats (at doses of 2,000.0–6,000.0 mg/kg body weight) and mice (at doses of 1,000.0–3,000.0 mg/kg body weight) were refusals of food and water, loss of coordination, sitting in one place, a dose-dependent increase in depression with subsequent complete depression, lack of response to external stimuli and death on the first or fourth day after administration. During autopsy in rats and mice that died as a result of poisoning with the drug ‘MEGASTOP for dogs’, we recorded pallor of the mucous membranes of the mouth, trachea, pharynx, and esophagus; increase in heart volume, atrial blood supply; pulmonary hyperemia; uncoagulated blood; increase in liver volume, dark cherry color, flabby consistency; catarrhal inflammation of the mucous membrane of the small intestine. According to the results of determining the parameters of acute toxicity of the drug ‘MEGASTOP for dogs’ in the case of a single intragastric injection, LD50 for male rats is 3,384.98 ± 444.94 mg/kg, and for female mice — 2,025.88 ± 279.46 mg/kg body weight, which allows to classify it to class IV by the toxicity — low-toxic substances (LD50 — 501–5,000 mg/kg) and by the degree of danger to class III— moderately dangerous substances (LD50 — 151–5,000 mg/kg)

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