scholarly journals STUDY OF THE CUMULATIVE PROPERTIES OF «INDEZ» ON LABORATORY WHITE RATES

2020 ◽  
Vol 21 (1) ◽  
pp. 98-104
Author(s):  
I. Ja. Kotsyumbas ◽  
O. M. Brezvyn ◽  
Y. A. Ivashkiv ◽  
H. V. Rudyk ◽  
Ju. V. Muzika
Keyword(s):  
Body Weight ◽  
Total Dose ◽  
Animal Husbandry ◽  
Bactericidal Effect ◽  
Laboratory Animals ◽  
Control Group ◽  
White Rats ◽  
Weight One ◽  
Iron Sulphate ◽  
Spleen Mass

The article presents the results of the study of «Indez» disinfectant. An effective disinfectant for use in animal husbandry should be easy to use, be non-toxic, have broad bactericidal spectrum, be non-carcinogenic, have non-addictive micro-flora and provide a permanent bactericidal effect in the presence of animals, while sanitizing the air environment. «Indez» disinfectant is a small, amorphous grey powder with a specific odour, well sprayed. It is composed of triiodomethane (iodoform), zinc oxide, iron sulphate (II) (iron sulphate), copper sulphate, silicon dioxide, zeolite, active essential oils, a complex of surfactants and pH regulators, auxiliaries. This preparation can be used in the presence of animals; the disinfectant effect is based on the spectrum of antimicrobial action of its constituents. In the experiment of the study of the cumulative properties of «Indez» 80 white rats weighing 180-200 ± 10 g, tested doses of 1/5, 1/10, 1/20, from DL50 were used. To the animals of the experimental group, the suspension of the preparation was administered orally: in the first 4 days - 1/5 of DL50, then 1/10 and 1/20. Animals of the control group were administered saline at a dose of 0.5 ml. Observations on rats were carried out for 22 days. Depending on the dose of the drug, the cumulation coefficient. As a result of the study of the cumulative properties of the drug it is established that the introduction of a total dose of «Indez» 56,8 cm3 per 1 kg body weight does not cause death of white rats. When conducting a total dose of 63,4 cm3 / kg body weight one animal died, representing 5 %. Further administration of the drug lethality was on the 18-th day (total dose 83,13 cm3 / kg) – 15 %, on the 19-th day (total dose 93,0 cm3 / kg) – 20 % and the 20-th day (total dose 102,87 cm3 / kg) – 45 %. With an increase of 9,873 1,5 times (1,8095 cm3 / kg) on the 21-st day, the mortality rate was 80 %, and on the 22-nd day of the studies 90 % of laboratory animals were killed, the total dose was 1032,49 cm3 / kg. Under the study of the cumulative properties of «Indez» disinfectant, it was found that the cumulation coefficient in rats is 2,2 units. This, in turn, indicates that the test agent has moderately pronounced properties for cumulation. In this case, white rats inhibit the hematopoietic function of the bone marrow and reduce the body’s defences, as evidenced by a probable decrease in leukocyte count and a slight decrease in haemoglobin, lymphocyte count, spleen mass factor, and increased segmentation.

scholarly journals Study of the parameters of acute toxicity of the drug “Devimectin 1 %” with a single subcutaneous injection in white rats

2020 ◽  
Vol 22 (100) ◽  
pp. 28-31
Author(s):  
Yu. R. Hunchak ◽  
B. V. Gutyj ◽  
R. M. Sachuk ◽  
Ya. S. Stravsky
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Subcutaneous Administration ◽  
Female Rats ◽  
Laboratory Animals ◽  
Control Group ◽  
Main Experiment ◽  
Sterile Saline ◽  
White Rats ◽  
Therapeutic Properties

In the study of the drug for injectable use – “Devimectin 1 %”, together with the confirmation of therapeutic properties, it is necessary to determine the LD50 obtained in the study of acute toxicity. The aim of the work was to study the acute toxicity of “Devimectin 1 %” in white rats by injection. To fulfill this goal on the principle of analogues was formed control and three experimental groups of 4 animals each (n = 4). The drug was administered in doses of 5000.0; 10000.0; 20000.0 mg/kg body weight in absolute weight of the drug once subcutaneously in the withers. The control animals were injected subcutaneously with sterile saline 1.0 cm3. After taking into account the results of the previous experiment in the main experiment, 7 experimental groups were formed, whose rats were injected subcutaneously with “Devimectin 1 %” in doses of 5000.0; 7500.0; 10000.0; 12500.0; 15000.0; 17500.0 and 20000.0 mg/kg body weight, as well as a control group to which animals were injected with sterile saline with a volume of 1.0 cm3. There were 6 animals in each group (n = 6). It was found that for the administration of the drug at a dose of 5000 mg / kg body weight, no animal died, for 10000.0 and 20000.0 mg/kg body weight, respectively, one and 4 animals died. Death occurred for 2–6 days depending on the administered dose. In the main experiment with subcutaneous administration of “Devimectin 1 %” at a dose of 5000.0 mg/kg body weight during the 14-day period of the study, no animal died; for the introduction of the drug at a dose of 7500.0 mg/kg killed one animal; for 10000.0 – 2; for 12500.0 and 15000.0 – 3 rats; for 17500.0 – 5 rats and for the introduction of the drug at a dose of 20000.0 mg/kg body weight, all experimental animals died. The death of laboratory animals occurred for 2–6 days depending on the administered dose. According to the results of studies, it was found that the LD50 of the drug “Devimectin 1 %” under the conditions of its single subcutaneous administration to female rats is 12881.20 ± 1390.54 mg/kg, LD10 – 5978.43 mg/kg, LD16 – 7495.68 mg/kg, LD84 – 18266.73 mg/kg, LD90 – 19783.98 mg/kg, LD100 – 20959.49 mg/kg body weight, respectively. Therefore, the drug “Devimectin 1%” when administered subcutaneously can be classified as toxicity class VI – substances relatively harmless (LD50subcut> 4500,0 mg/kg). Further studies will be the next step in pre-registration trials to examine the subacute toxicity of “Devimectin 1 %”.

TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

1970 ◽  
pp. 67-69
Author(s):  
E. K. Rakhmatullin ◽  
O. D. Sklyarov
Keyword(s):  
Drug Toxicity ◽  
Probit Analysis ◽  
Laboratory Animals ◽  
Control Group ◽  
Hazard Class ◽  
Water Ingestion ◽  
White Rats ◽  
Poorly Soluble ◽  
And Behavior ◽  
Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

scholarly journals Preclinical evaluation of the veterinary drug “Amoxidev 15” for its use in surgical and obstetric practice

2021 ◽  
Vol 23 (103) ◽  
pp. 109-115
Author(s):  
L.-M. Kostyshyn ◽  
R. Sachuk ◽  
Ye. Kostyshyn ◽  
O. Katsaraba
Keyword(s):  
Body Weight ◽  
Soft Tissues ◽  
Control Level ◽  
Subcutaneous Administration ◽  
The Body ◽  
Laboratory Animals ◽  
Veterinary Drug ◽  
Intragastric Administration ◽  
Toxicological Evaluation ◽  
White Rats

Suspension for injection “Amoxidev 15” is prescribed to fur-bearing animals (mink, fox), dogs and cats for the treatment of respiratory diseases (tonsillitis, tracheitis, pneumonia, bronchitis, rhinitis, sinusitis, bronchopneumonia), digestive (gastritis, enteritis, enteritis). genitourinary systems (nephritis, urethritis, urocystitis, mastitis, metritis, agalactia), musculoskeletal system (arthritis, osteoarthritis, joint injuries, tendonitis, hoof lesions), skin and soft tissues (eczema, dermatitis) caused by sensitive drug by microorganisms, including colibacillosis, streptococcus, bronchopneumonia, etc. Toxicological evaluation of the veterinary drug “Amoxidev 15” under the conditions of acute and subacute toxicological experiments on a model of white rats. According to the results of an acute toxicological experiment with intragastric administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration. The maximum administered dose (in absolute weight of the drug) was 20000.0 mg/kg body weight, which allows to refer the drug to class VI toxicity of relatively harmless substances (DL50 > 15000 mg/kg body weight), and the degree of safety to class IV – low-hazard substances (DL50 > 5000 mg/kg). According to the results of an acute toxicological experiment with subcutaneous administration of the drug “Amoxidev 15” white rats DL50 could not be calculated because the death of laboratory animals was not detected within 14 days after administration, the maximum dose was 5000.0 mg/kg body weight, therefore, the drug “Amoxidev 15” when administered subcutaneously by toxicity can be classified as class VI substances relatively harmless (DL50 Subcut > 4500.0 mg/kg). When administered subcutaneously to white rats, the drug “Amoxidev 15” under conditions of subacute toxicological experiment in doses of 0.1–1.0 ml/kg does not cause hemo-, hepato- and nephrotoxic effects on the body of laboratory animals, although 3-day administration of the drug in a dose 1.0 ml/kg body weight caused an increase in the activity of hepatospecific enzymes ALT and AST by 12.5 and 11.1 % (P < 0.05), respectively, relative to the control, which was restored to the control level 7 days after cessation.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF VERNONIA AMYGDALINA LEAF ETHANOLIC EXTRACT IN WHITE RATS INDUCED BY PARACETAMOL

2018 ◽  
Vol 11 (10) ◽  
pp. 562
Author(s):  
Panal Sitorus ◽  
Nerdy Nerdy
Keyword(s):  
Body Weight ◽  
Methyl Cellulose ◽  
Ethanolic Extract ◽  
Scientific Data ◽  
Control Group ◽  
Carboxy Methyl Cellulose ◽  
Vernonia Amygdalina ◽  
White Rats ◽  
Significant Difference ◽  
Carboxy Methyl

Objectives: A study on the hepatoprotective effect of Vernonia amygdalina leaves ethanolic extract on white rats induced by paracetamol was aimed to obtaining scientific data and evidence the hepatoprotective ability of the extract.Methods: The research was a complete randomized design, using 36 white rats which were divided into 6 groups. The normal control group was given 1% carboxy methyl cellulose for 7 days and followed by distilled water 8 hours after treatment. The positive control group was given 100 mg curcumin per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The negative control group was given 1% carboxy methyl cellulose for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The test group was given 50, 100 and 200 mg extract per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. Blood sampling was carried out 3 times (before treatment, after treatment and after induction).Results: With doses of 50, 100, and 200 mg extract per kg body weight of rats give significant difference on the decrease of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels after paracetamol administration.Conclusions: It can be concluded that Vernonia amygdalina leaves ethanolic extract were potentially hepatoprotective.

scholarly journals THE THE TERATOGENIC EFFECTS OF ETHANOLIC EXTRACT OF BINTANGUR LEAVES (CALOPHYLLUM SOULATTRI BURM. F) ON FEMALE WHITE RATS

2019 ◽  
pp. 160-163
Author(s):  
INARAH FAJRIATY ◽  
HAFRIZAL RIZA ◽  
FAJAR NUGRAHA ◽  
FRENGKI FRIANTO
Keyword(s):  
Body Weight ◽  
Comparison Group ◽  
Ethanol Extract ◽  
Teratogenic Effect ◽  
Control Group ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
White Rats ◽  
Significant Difference ◽  
Skeletal Formation

Objectives: Drugs can cause undesired effects on the fetus during pregnancy, especially embryonic/organogenesis which could lead to defects in the fetus because some types of drugs can penetrate the placenta and will undergo biotransformation into a highly reactive compound that has the potential to become a teratogenic compound. The aim of this research was to examine the teratogenic effect of bintangur leaves (Calophyllum soulattri Burm. F) ethanol extract to Sprague Dawley strain white rats. Methods: The white rats are divided into four treatment groups: Control group was given carboxymethyl cellulose Na 1%, comparison group was given trimethoprim 360 mg/kg BW, C. soulattri leaves ethanol extract (CLE) 100 mg/kg BW, and CLE 500 mg/kg BW. The treatment was administrated since organogenesis period. Cesarian section was performed to pregnant rat at the 20th day to separate the fetuses. Observation covered body weight of pregnant rats, fetal biometric, morphological malformation, and skeletal formation. Results: CLE 100 mg/kg BW and 500 mg/kg BW did not cause any change in the number of a living fetus, body weight, and length of fetuses like the comparison group. Both doses of CLE shown have a normal skeletal formation. Resorption was found in the comparison group and CLE 100 mg/kg BW with the percentage was 65.21% and 6.67%. It was found that there is no significant difference (p<0.05) between both doses of CLE compared to control group. Conclusion: From the results, it is concluded that CLE did not have the teratogenic effect.

scholarly journals EFEK PAPARAN DEKOK BIJI PINANG (Areca catechu) TERHADAP MOTILITAS SPERMATOZOA TIKUS (Rattus norvegicus): UPAYA MENEMUKAN KANDIDAT ANTIFERTILITAS PRIA (The Effect of Betel Nut Extract (Areca catechu) on Mice Spermatozoa Motility (Rattus norvegicus: Effort to Find Out Man Antifertility Candidate)

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Muslim Akmal
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Betel Nut ◽  
Spermatozoa Motility ◽  
White Rats ◽  
Areca Catechu ◽  
Different Doses

This research was conducted to prove the ability of betel nut extract (Areca catechu) in causingdegradation of sperm motility on rats (Rattus norvegicus) strain Wistar. This research used 2-3 monthsage, 200-250 g body weight of male white rats Rattus norvegicus strain Wistar. The rats were dividedinto 5 groups in equal number, 3 rats each group. They were a control group without treatment and 4treatment groups which were given different doses of betel nut extract for 7 days, i.e., 1, 2, 3, and 4 g/200g body weight respectively. The result showed that increasing doses of betel nut extract can result indegradation of sperm motility significantly (P0.05).Keywords: Areca catechu, Rattus norvegicus, sperm motility

scholarly journals Combined effect of glyphosphate, saccharin and sodium benzoate on rats

2018 ◽  
Vol 9 (4) ◽  
pp. 591-597 ◽  
Author(s):  
M. A. Lieshchova ◽  
N. M. Tishkina ◽  
A. A. Bohomaz ◽  
P. M. Gavrilin ◽  
V. V. Brygadyrenko
Keyword(s):  
Body Weight ◽  
Sodium Benzoate ◽  
Food Additives ◽  
Combined Effect ◽  
Male Rats ◽  
Laboratory Animals ◽  
Relative Mass ◽  
Control Group ◽  
Expiry Date ◽  
The Impact

Herbicides and food additives are included in many food products for humans. Non-used products or products beyond their expiry date are deposited in places of utilizatioin of solid municipal wastes, where they can take effects on the organisms of mouse-like rodents. Among the herbicides, glyphosphate takes first place in the world for volume of production, and is the most intensely used in agricultural farming. The discussion about negative impact on the organisms of mammals, especially against the background of using various substances and environmental factors, continues. In this study, we determined the combined effect of glyphosphate and food additives on the organism of laboratory animals, which manifested in changes in body weight, condition and indices of mass of the internal organs and blood parameters. Four groups of laboratory male rats were formed, which over 42 days received: unlimited access to clean water; 1% aqueous solution of glyphosate; 1% solution of glyphosphate and 1% solution sodium benzoate; 1% solution of glyphosphate with 1% solution of saccharin. Glyphosphate and glyphosphate with sodium benzoate and saccharin significantly reduced the daily increases in body weight of animals compared to the control group. The studied substances have notable suppressive effect on the immune system and haematopoiesis in general, which is manifested in reduce of relative mass of the thymus and spleen against the background of increase in the amount of lymphocytes in the peripheral blood. The inhibition of haematopoiesis is indicated by decrease in the amount of erythrocytes, neurophils and hemoglobin of blood of animals from the experimental groups. The impact on the digestive system of glyphosphate and food additives is indicated by occurance of the effect of “irritation” of mucous membranes, and, as a result, disorders in absorption followed by the disorder in metabolic processes. A dysbalance occurs in enzymic systems of the organism, which is manifested in distrophic processes, especially in the liver parenchyma, indicated by the activity of blood enzymes (ALT, AST, alkaline phosphatase), total number and ratio of proteins of blood plasma. We determined the impact of glyphosphate and its mixes with benzoate and saccharin on the pancreas, which manifests in severe pancreatitis with steep increase in the level of glucose of blood. The results of the study allow us to state that mixture of glyphosphate and food additives can cause toxic effect in animals and humans, which often contact with herbicides.

scholarly journals APPLICATION OF CELLULAR TECHNOLOGIES FOR REGENERATION OF PERIODONTAL TISSUES IN EXPERIMENT

2018 ◽  
Vol 62 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Sergei P. Rubnikovich ◽  
Igor D. Volotovsky ◽  
Yulia L. Denisova ◽  
Тatiana E. Vladimirskaya ◽  
Vasilina A. Andreeva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Morphological Changes ◽  
Fibrous Tissue ◽  
Laboratory Animals ◽  
Control Group ◽  
Cell Transplant ◽  
Periodontal Tissues ◽  
White Rats

A promising scope of modern scientific research is the use of autologous and allogeneic mesenchymal stem cells for regeneration of periodontal tissues. The aim of the study was to evaluate the nature of morphological changes in the pathologically altered periodontal tissues after injection of a biotransplant containing mesenchymal stem cells of the adipose tissue immobilized on a collagen carrier in an animal experiment. In the experiment, 60 randobbred females of white rats were used as a model, whose adipose tissue was taken to obtain allogenic mesenchymal stem cells. All animals were divided into 5 groups, depending on the planned method of treatment – 10 rats each. The control group consisted of 10 laboratory animals with healthy gingiva. The experimental gingival recession model was created by the V-shaped excision of periodontal tissues. The bioplastic collagen material “Collost” gel 7 % in isolated form determines the fibrosis intensification and serves as a “matrix” for the formation of a fibrous tissue, ensures the adhesion of stem cells and their transformation into pro- and fibroblasts. Injection of a cell transplant suspension into physiological saline activates the processes of cell proliferation and transformation of fibroblast differentiating cells. Suspension of the cell transplant on a sterile bioplastic collagen material “Collost” gel 7 % enhances the effects of gel and stem cells, promotes the leveling of atrophic and dystrophic changes in the gum, strengthening a mechanical component, reducing the recession of the gum and the depth of the gingival pocket.

scholarly journals The effect of the phenolic and other active compounds of the alcohol and acid extracts of cinnamon on some of the Biochemical Parameters of induced diabetes mellitus

2015 ◽  
Vol 9 (1) ◽  
pp. 24-36
Author(s):  
Hayder Turki AL-Musaw AL-Musawi ◽  
Mohammed Ibrahem AL-Tai AL-Tai ◽  
Muna Turki AL-Musawi AL-Musawi
Keyword(s):  
Body Weight ◽  
Density Lipoprotein ◽  
Treated Group ◽  
Laboratory Animals ◽  
Control Group ◽  
Alcoholic Extract ◽  
Serum Concentrations ◽  
Active Compounds ◽  
Cinnamon Extract ◽  
Long Duration

The phenolic compound and other active compounds were extracted from cinnamon, in anattempt to produce a drug from Medical and herbal Plants for the diabetes on rats. Theextraction process included two methods the first carrid out with 2% v/v acetic acid andpropanol, and in the second process 70% v/v ethanol and chloroform were used. In the detectionof extracts it was noticed that both conteined phenols, flavonoids, tannins and Glycosides andCoumarins, while the saponin showed up only in the alcoholic extract of cinnamon. The phenoliccompounds in the extracts of cinnamon were determined by using Ultraviolet Spectroscopy (UV)and other function groups such as phenols, aldehyedes, estars, hydrocarbons, alcohols andcarboxylic compounds were diagnosed by using Infrared spectroscopy (IR). The study of the halflethal dose (midl lethal) (LD50) was also examined by testing the toxicity of the two cinnamon 2015 مجلة مركز بحوث التقنيات االحيائية المجلد التاسع- العدد االول42extracts. Rats were used as laboratory animals in conducting the experiments. The rats were fedby the dose (100, 200, 300,400, 500 mg / kg of body weight). The results have shown that there isno toxic effect in the cinnamon extracts. Similar concentrations were used to determine theeffective dose of the extracts in reducing the level of glucose in the blood of healthy animals. Thedose (300) mg / kg of body weight of the animal body has proved to be the the most efficient as itdecreased. The fasting glucose (Glu) serum consider by following of the cinnamon extracts withthe adopted groups during the duration of (30) days in comparison with the control group (theinfected) and outperformed the treated given Glibenelinide (daonil) group. The biochemical testsshowed that the serum concentrations of each of cholesterol (TC),Triglyceride (TG), low densitylipoprotein (LDL-C) was lowered in each of the groups treated with both cinnamon extracts incomparison with the positive groups (infected) throughout the duration (30) days and overcomethe daonil treated group. However the serum high density lipoprotein (HDL-C) level wasincreased in cinnamon extract treated groups and decreased in the control group (infected).The serum concentrations of enzymes (GOT) and (GPT) were measured to test the liver functionafter where their levels were lowered after (30) days in the cinnamon extracts administeredgroups compared with the two other groups, the control (positive) and the Daonil. The Urea andCreatinine levels were measured to determine or test the kidney functions, where it was observedas from the early days of the infection in that animals infected by induced diabetes developedsymptoms of the illness, apparent in the high levels of urine which affected the functions ofkidney, but the long duration (30 days) of treatment with these extracts of cinnamon proved theirefficiency over the control group (positive).

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

2020 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
Bunga Rimta Barus
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Weight Group ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Sida Rhombifolia

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

Sign in / Sign up

Export Citation Format

Share Document