scholarly journals Penambahan Molases Untuk Meningkatkan Kualitas Amoniasi Jerami Padi dan Pengaruhnya terhadap Produk Fermentasi Rumen Secara In-Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 27-33
Author(s):  
Muhamad Bata ◽  
Nur Hidayat
Keyword(s):  
Protein Synthesis ◽  
Orthogonal Polynomial ◽  
Rice Straw ◽  
Rumen Fluid ◽  
Research Result ◽  
Nutrient Content ◽  
Optimal Level ◽  
Variance Analysis

Supplementation of molasses to improve the quality of rice straw ammonization and its effect on fermentation product in-vitroABSTRACT. Aimed of this research was to find out the optimal level of molasses addition at straw rice ammonization process to N-NH3 production, VFA and microbe protein synthesis. Material used was rumen fluid of fistula cattle, rice straw, water, urea and molasses. Treatment tried was level of molasses addition 0%, 15% and 30% on rice straw which given urea. Research was carried out by experimental method as in vitro, was conducted use completely randomized design. Variable measured were N-NH3, VFA and microbe protein synthesis. Intake data entered in data tabulation and analyzed variance then continued by orthogonal polynomial test. Research result after ammonization indicated that acidity level and concentration of released NH3 decrease parallel with addition of molasses level, and also increase the nutrient content which was crude protein increase and crude fiber decrease. Variance analysis and Test of orthogonal polynomial result indicated that treatment of molasses addition have highly significant effect (P0.01) and linier respond to concentration of released NH3 after ammonization. Research result as in vitro indicated concentration N-NH3 and VFA total decrease while microbe protein synthesis increase. Variance analysis result indicated that molasses addition treatment at straw rice ammonization process have highly significant (P 0.01) on concentration of N-NH3, VFA total and Microbe Protein Synthesis. Test of orthogonal polynomial for molasses addition at straw rice ammonization process indicated linier respond on concentration of NH3 N-NH3 and VFA total, but microbe protein synthesis quadratic had respond (P 0,01) white regression equation Y= 52.187-1.089222X + 0.11X2 (r2) 87.27 and (r) 0.9341. Research result could conclude that molasses addition up to level 30% able to improve quality of straw rice ammonization process by NH3 fixation so that increase nutrient ingredient, decrease NH3 that lost to atmosphere, improve utilization of N-NH3 and VFA and also increase microbe protein synthesis.

scholarly journals Pengaruh Molases Pada Amoniasi Jerami Padi Menggunakan Urea Terhadap Kecernaan Bahan Kering dan Bahan Organik In Vitro

2008 ◽  
Vol 8 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Muhamad Bata
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Research Result ◽  
Optimal Level ◽  
Improve Quality ◽  
Dry Matter Digestibility ◽  
Organic Matter Digestibility

The effect of molasses on ammoniated straw by using urea on dry and organic matter digestibility as in vitroABSTRACT. Aimed of this research was to find out the optimal level of molasses addition to improve quality, dry matter and organic matter digestibility of rice straw ammonization process. Materials used were rumen fluid of fistula cattle, grind of rice straw, water, urea and molasses. Research designed used Completely Randomized Design (CRD). As treatments were R0: rice straw 1000 g dry matter + 500 g water + 50 g urea + 0 percent of molasses, R1: R0 + 15 percent of molasses, R2: R0 + 30 percent of molasses. Urea and molasses dissolved in water and then entered into pollybag. All pollybag observe and let for 15 days, each treatment replicated 6 times. Variable measured were dry matter digestibility and organic matter digestibility. Research result showed that ammonization product of NH3, Acidity Level and crude fiber having decreased while crude protein content increased. Variance analysis indicated that treatments had significant effect (P0.05) on dry matter and organic matter digestibility. Orthogonal polynomial test indicated that level of molasses increase (P0.05) of dry matter and organic matter digestibility linearly. It can be concluded that addition up to 30 percent in ammoniating of rice straw using urea can improve quality of ammonization and increasing dry matter and organic matter digestibility.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P < 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

Variation in the in vitro hydrolytic activity of rumen and faecal inocula

2002 ◽  
Vol 2002 ◽  
pp. 166-166 ◽  
Author(s):  
M. Afdal ◽  
F.L. Mould ◽  
C. Rymer ◽  
E. Owen ◽  
D.I. Givens
Keyword(s):  
Rumen Fluid ◽  
Hydrolytic Activity ◽  
Potential Method ◽  
Gas Production ◽  
Microbial Populations ◽  
Fluid Medium ◽  
Faecal Material ◽  
Evaluation Systems

Considerable efforts have been made regarding the use of faecal material to provide a microbial inoculum for in vitro feed evaluation systems. However total gas production, rate of gas release and the extent of degradation of feeds incubated using faecal inoculum are lower than those incubated in a rumen fluid medium. It has been suggested that this is due to lower microbial activity, a consequence of the different microflora and reduced microbial numbers (e.g. Mauricio, 1999). Microbial populations are dynamic so, as their enzyme activity profiles change rapidly, little information is obtained from examining these. However, their hydrolytic activity as reflected by their ability to degrade specific substrates can be simply measured and provides a potential method with which to assess the quality of inocula with respect to their use in in vitro systems. The data presented here are from a larger study in which the differences between the hydrolytic activity of faecal material and rumen contents as influenced by the time of sampling were assessed in vitro.

scholarly journals PENGARUH DAUN TURI (Sesbania grandiflora) DAN LAMTORO (Leucaena leucocephala) DALAM RANSUM SAPI BERBASIS INDEKS SINKRONISASI PROTEIN - ENERGI TERHADAP SINTESIS PROTEIN MIKROBA RUMEN

Pastura ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 47
Author(s):  
Afduha Nurus Syamsi ◽  
Fransisca Maria Suhartati ◽  
Wardhana Suryapratama
Keyword(s):  
Protein Synthesis ◽  
Rumen Fluid ◽  
Microbial Protein ◽  
In Vitro Techniques ◽  
Microbial Protein Synthesis ◽  
Sesbania Grandiflora ◽  
Protein Energy ◽  
Randomized Design ◽  
Better Than

An experiment was aimed to assess the use of the legume leaf as a source of protein feedstuff and levels of synchronization protein-energy (SPE) index in the diet of cattles on ammonia (N-NH3) and microbial protein synthesis (MPS). In vitro techniques was done. The research was used a completely randomized design (CRD), with factorially pattern (2x3), the first factor was the two species of legume (Sesbania leaves and Leucaena leaves) and the second factor was the three level of the SPE index (0.4, 0.5, and 0.6), there were 6 treatment combinations and each was 4 replicates. The results showed that no interaction between legume with SPE index, but each factor was significantly effect (P<0.05) on N-NH3 of rumen fluid and MPS. The research concluded that Leucaena leaf is a legume that is better than Sesbania leaf in terms of their ability toincrease MPS. SPE index is the best in producing MPS at level 0.6. Key words: Legume, synchronization of protein and energy index, ammonia, microbial protein synthesis

scholarly journals Peningkatan Kualitas Jerami Padi dan Pengaruhnya Terhadap Kecernaan Nutrien dan Produk Fermentasi Rumen Kerbau dengan Feces Sebagai Sumber Inokulum

2013 ◽  
Vol 13 (2) ◽  
pp. 59-67 ◽  
Author(s):  
Syapura Syapura ◽  
Muhamad Bata ◽  
Wardhana Surya Pratama
Keyword(s):  
Protein Synthesis ◽  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
Microbial Protein ◽  
Fermentation Products ◽  
Microbial Protein Synthesis ◽  
Organic Matter Digestibility

Improving of rice straw quality and its effect on ability nutrient digestibility and rumen metabolism products of buffalo in-vitro with feces as inoculum source ABSTRACT.  This study was aimed to determine the effect of feeding  ammoniated rice straw plus concentrate on buffalo nutrient digestibility and rumen fermentation products by in vitro. The Research was carried out by using  experimental method, designed according to completely  randomized design (CRD). The source of inoculum was obtain from different feces of three  buffalos kept in  Datar Village of Purwokerto region fed  rice straw, rice straw plus concentrate and rice straw ammoniated plus concentrate with dry matter ratio of 80 : 20. The treatments tested consisted of three treatments, namely R0 =  control feed using rice straw; R1 = the use of rice straw plus concentrate with a ratio of  (DM basis) 80:20; R2 = the use of ammoniated rice straw plus concentrate with a ratio of (DM basis) 80:20. The treatments were repeated 7 times, so there were 21 experimental units. The Variables measured included total VFA, Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS),   Dry Matter and Organic Matter Digestibility. The result of this study showed that the treatment had an effect significant (P0.05) on the concentration of VFA, Ratio A/P,  N-NH3,  Microbial Protein Synthesis (MPS), and Dry Matter and Organic Matter Digestibility. The HSD test showed that the highest production of  VFA,Ratio A/P, N-NH3, Microbial Protein Synthesis (MPS), Dry Matter and Organic Matter Digestibility were achieved at R2 followed by R1 and R0 respectively. The conclusion is that the ammoniated rice straw supplemented with concentrate can be recommended to be fed to buffalo

scholarly journals Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro

2017 ◽  
Vol 13 (1) ◽  
pp. 57
Author(s):  
Joko Riyanto ◽  
Endang Baliarti ◽  
Lies Mira Yusiati ◽  
Tety Hartatik ◽  
Diah Tri Widayati
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
Nutrient Content ◽  
Protective Material ◽  
Randomized Design ◽  
The Difference ◽  
Poly Unsaturated Fatty Acid

<p>This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA.</p><p> </p><p>Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection</p>

scholarly journals Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro

2017 ◽  
Vol 13 (1) ◽  
pp. 57
Author(s):  
Joko Riyanto ◽  
Endang Baliarti ◽  
Lies Mira Yusiati ◽  
Tety Hartatik ◽  
Diah Tri Widayati
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
Nutrient Content ◽  
Protective Material ◽  
Randomized Design ◽  
The Difference ◽  
Poly Unsaturated Fatty Acid

<p>This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA.</p><p> </p><p>Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection</p>

scholarly journals Rumen protein degradation and biosynthesis

1983 ◽  
Vol 50 (3) ◽  
pp. 569-582 ◽  
Author(s):  
L. Raab ◽  
B. Cafantaris ◽  
T. Jilg ◽  
K. H. Menke
Keyword(s):  
Protein Synthesis ◽  
Protein Degradation ◽  
Rumen Fluid ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Total N ◽  
Fluid Medium ◽  
The Difference ◽  
Fermentable Carbohydrates

1. A method is described for the determination of protein degradation based on measurements of ammonia concentration and gas production (Menke et al. 1979) when a feedingstuff was incubated with rumen fluid in vitro.2. NH3 liberated during incubation is in part used for microbial protein synthesis. Production of carbon dioxide and methane can be regarded as a measure of energy available for protein synthesis. The ratio, gas production: incorporation of NH3-nitrogen was estimated by addition of starch to the substrate. The response in gas production was linear in the range 0–200 mg starch, when starch was added to 0–200mg feedingstuff dry matter and 30 ml rumen fluid-medium mixture.3. Linear regression between NH3-N concentration (y, mg) and gas production (x, ml) yielded an intercept (b0) representing that amountof NH3-N which would be released when no fermentable carbohydrates were available and consequently no bacterial protein synthesis took place.4. The difference between this intercept b0 and NH3-N content in the blank (rumen fluid without substrate added) indicated the amount of NH3 liberated from protein and other N-containing compounds of the feedingstuff incubated. In vitro-degradable N (IVDN) was calculated as a proportion of total N by the equation:

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