scholarly journals Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro

2017 ◽  
Vol 13 (1) ◽  
pp. 57
Author(s):  
Joko Riyanto ◽  
Endang Baliarti ◽  
Lies Mira Yusiati ◽  
Tety Hartatik ◽  
Diah Tri Widayati
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
Nutrient Content ◽  
Protective Material ◽  
Randomized Design ◽  
The Difference ◽  
Poly Unsaturated Fatty Acid

<p>This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA.</p><p> </p><p>Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection</p>

scholarly journals Pengaruh Proteksi Aldehid Untuk Meloloskan Poly Unsaturated Fatty Acid Pada Menir Kedelai dan Minyak Ikan Lemuru Secara In Vitro

2017 ◽  
Vol 13 (1) ◽  
pp. 57
Author(s):  
Joko Riyanto ◽  
Endang Baliarti ◽  
Lies Mira Yusiati ◽  
Tety Hartatik ◽  
Diah Tri Widayati
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
Nutrient Content ◽  
Protective Material ◽  
Randomized Design ◽  
The Difference ◽  
Poly Unsaturated Fatty Acid

<p>This research was aims to determine the effect of formaldehyde using as a protective material in the soy groats and lemuru fish oil mixtured to escape of the protein and poly unsaturated fatty acid (PUFA) by in vitro. The rumen fluid was derived from donor of the Ongole crosbred fistulated as much as 3 heads. The experimental design used completely randomized design factorial 2x3 pattern consists of two factors:the first factor (soybean groats:lemuru fish oil in the ratio 2:1 and 4:1) and the second factor (the use of formaldehyde at levels of 0%, 2% and 4% base on the dry matter) and was repeated 5 times. The parameters are consist of the nutrient content, the substrates soybean groats and lemuru fish oil the rumen fluid fermented PUFA content by in vitro. Results of the study are levels of protein, fatty acids  oleic, linoleic and arachidonic much available on soybean groats:lemuru fish oil protected formaldehyde at levels of 2% and 4%. The difference between the ratio of soybean groats with lemuru fish oil had no significant (P≥0,05) but significantly different at different levels of formaldehyde (P≤0,05). The conclusion are formaldehyde content of 37% with a level of 2% can be used for protection the soybean groats and lemuru fish oil with a ratio of 4:1 and is able to provide protein as well PUFA.</p><p> </p><p>Keywords: soy groats, lemuru fish oil, in vitro, PUFA, formaldehyde protection</p>

scholarly journals MINIMIZE THE HYDROGENATION OF UNSATURATED FATTY ACID IN RUMEN WITH FORMALDEHYDE

2011 ◽  
Vol 11 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Nafly C. Tiven ◽  
Lies Mira Yusiati ◽  
Rusman Rusman ◽  
Umar Santoso
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Rumen Fluid ◽  
In Vitro Fermentation ◽  
Rumen Microbes ◽  
The Difference ◽  
Female Sheep ◽  
Test Result ◽  
Range Test

This research aimed to know the ability of formaldehyde to protect unsaturated fatty acid of CPO on the hydrogenation process by rumen microbes. In this experiment, the in vitro fermentation of rumen fluid was carrying out. It was taken from the rumen-trocar of female sheep. The unsaturated fatty acid source was from CPO (Crude Palm Oil) which encapsulated by formaldehyde 37% within 0%, 1%, 2% and 3%. The data was analyzed by Completely Random Design with Duncan's New Multiple Range Test. The difference of means the treatments were tested by Duncan's New Multiple Range Test. Result showed that oleic and linoleic resulting from fermenting CPO protected by formaldehyde was increase if it was compared with the unprotected CPO. It can be concluded that encapsulated CPO with formaldehyde was able to prevent hydrogenating of unsaturated fatty acid, mainly oleic and linoleic.

scholarly journals MINIMIZE THE HYDROGENATION OF UNSATURATED FATTY ACID IN THE RUMEN WITH KAFFIR LIME (Citrus hystrix)

2017 ◽  
Vol 41 (3) ◽  
pp. 265
Author(s):  
Nafly Comilo Tiven
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Rumen Fluid ◽  
Skim Milk ◽  
Dry Weight ◽  
Rumen Microbes ◽  
Cooking Oil ◽  
The Difference

This research aimed to know the ability of citronella from C. hystrix to protect unsaturated fatty acid of Tropical cooking oil on the hydrogenation process by rumen microbes. The in vitro fermentation test used rumen fluid of local female sheeps as source of microbial, Tropical cooking oil as a source of unsaturated fatty acids and C. hystrix leaves as source of cironela. Tropical cooking oil was mixed with Preston skim milk (1:2). The mixture were added by C. hystrix leaves with the level of 0%, 1%, 2%, 3% and 4% (dry weight bases) from the weight of the mixture, then mixed evenly into protected Tropical cooking oil. The data was analyzed by Completely Random Design. The difference of means the treatments were tested by Duncan’s New Multiple Range Test. Result showed that oleic and linoleic resulting from fermenting Tropical cooking oil protected by citronela in C. hystrix was increase if it compare to unprotected. It can be concluded that Tropical cooking oil protected with citronelal C. hystrix was reduced  hydrogenation of unsaturated fatty acid, mainly oleic and linoleic.

scholarly journals EFFECT OF CRUDE PALM OIL PROTECTION WITH FORMALDEHYDE ON HYDROGENATION OF RUMEN FLUID UNSATURATED FATTY ACID: ITS EFFECT ON BLOOD AND MEAT FATTY ACID

2013 ◽  
Vol 13 (2) ◽  
pp. 142-148
Author(s):  
Nafly C. Tiven ◽  
Lies Mira Yusiati ◽  
Rusman Rusman ◽  
Umar Santoso
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Palm Oil ◽  
Unsaturated Fatty Acids ◽  
Rumen Fluid ◽  
Nutrient Content ◽  
Crude Palm Oil ◽  
Randomized Design ◽  
Range Test ◽  
Basal Ration

This research aimed to determine the effect of crude palm oil protected with formaldehyde on the hydrogenation of unsaturated fatty acids in the rumen and its effect on blood and meat fatty acids. Fifteenth local male lambs aged 9-12 months weighing 14-17 kg, were divided into 3 groups ration treatment. The first group received only the basal ration (R0), the 2nd group received the basal ration and 3% CPO (R1), while the 3rd group received the basal ration and 3% CPO protected with 2% formaldehyde (R2). Basal feed consisted of 60% grass, 30% bran and 10% soybean meal, with the nutrient content of 62.98% TDN, 45.5% DM, 14.48% CP, 4.70% EE and 21.93% CF. Parameters observed were the fatty acid from rumen fluid, blood and meat of sheep. Data were analyzed by complete randomized design direction patterns. Differences between treatments were tested further using Duncan's New Multiple Range Test. The results showed that treatment of R2 can increase unsaturated fatty acids in the rumen, blood and meat (P

scholarly journals Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zheng Ma ◽  
Na Luo ◽  
Lu Liu ◽  
Huanxian Cui ◽  
Jing Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Regulatory Network ◽  
Total Lipid Content ◽  
Fatty Acid Transport ◽  
Lipid Deposition ◽  
Ppar Signaling ◽  
The Difference

Abstract Background A body distribution with high intramuscular fat and low abdominal fat is the ideal goal for broiler breeding. Preadipocytes with different origins have differences in terms of metabolism and gene expression. The transcriptome analysis performed in this study of intramuscular preadipocytes (DIMFPs) and adipose tissue-derived preadipocytes (DAFPs) aimed to explore the characteristics of lipid deposition in different chicken preadipocytes by dedifferentiation in vitro. Results Compared with DAFPs, the total lipid content in DIMFPs was reduced (P < 0.05). Moreover, 72 DEGs related to lipid metabolism were screened, which were involved in adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating its main contribution to the regulation of the difference in lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were downregulated, but the ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, and SLC27A6 genes were upregulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK, TGF-beta and calcium) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs was proposed based on the above information. Conclusions Our data suggested a difference in lipid deposition between DIMFPs and DAFPs of chickens in vitro and proposed a molecular regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and the optimization of body fat distribution in broilers.

scholarly journals Penambahan Molases Untuk Meningkatkan Kualitas Amoniasi Jerami Padi dan Pengaruhnya terhadap Produk Fermentasi Rumen Secara In-Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 27-33
Author(s):  
Muhamad Bata ◽  
Nur Hidayat
Keyword(s):  
Protein Synthesis ◽  
Orthogonal Polynomial ◽  
Rice Straw ◽  
Rumen Fluid ◽  
Research Result ◽  
Nutrient Content ◽  
Optimal Level ◽  
Variance Analysis

Supplementation of molasses to improve the quality of rice straw ammonization and its effect on fermentation product in-vitroABSTRACT. Aimed of this research was to find out the optimal level of molasses addition at straw rice ammonization process to N-NH3 production, VFA and microbe protein synthesis. Material used was rumen fluid of fistula cattle, rice straw, water, urea and molasses. Treatment tried was level of molasses addition 0%, 15% and 30% on rice straw which given urea. Research was carried out by experimental method as in vitro, was conducted use completely randomized design. Variable measured were N-NH3, VFA and microbe protein synthesis. Intake data entered in data tabulation and analyzed variance then continued by orthogonal polynomial test. Research result after ammonization indicated that acidity level and concentration of released NH3 decrease parallel with addition of molasses level, and also increase the nutrient content which was crude protein increase and crude fiber decrease. Variance analysis and Test of orthogonal polynomial result indicated that treatment of molasses addition have highly significant effect (P0.01) and linier respond to concentration of released NH3 after ammonization. Research result as in vitro indicated concentration N-NH3 and VFA total decrease while microbe protein synthesis increase. Variance analysis result indicated that molasses addition treatment at straw rice ammonization process have highly significant (P 0.01) on concentration of N-NH3, VFA total and Microbe Protein Synthesis. Test of orthogonal polynomial for molasses addition at straw rice ammonization process indicated linier respond on concentration of NH3 N-NH3 and VFA total, but microbe protein synthesis quadratic had respond (P 0,01) white regression equation Y= 52.187-1.089222X + 0.11X2 (r2) 87.27 and (r) 0.9341. Research result could conclude that molasses addition up to level 30% able to improve quality of straw rice ammonization process by NH3 fixation so that increase nutrient ingredient, decrease NH3 that lost to atmosphere, improve utilization of N-NH3 and VFA and also increase microbe protein synthesis.

scholarly journals Phytochemical and Antimicrobial Investigation of Latex from Euphorbia Abyssinica Gmel

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Fathy EL-Fiky ◽  
Kaleab Asres ◽  
Simon Gibbons ◽  
Hala Hammoda ◽  
Jihan Badr ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Oleanolic Acid ◽  
Unsaturated Fatty Acid ◽  
Methanol Extract ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Phytochemical Investigation ◽  
Antibacterial And Antifungal ◽  
Micro Organisms

Phytochemical investigation of the latex of Euphorbia abyssinica Gmel (Euphorbiaceae) afforded a new hydroxy unsaturated fatty acid, 8( R)-hydroxy-dec-3( E)-en-oic acid (1), in addition to the four known compounds lupeol (2), β-sitosterol (3), oleanolic acid (4) and β-sitosterol-3- O-glucoside (5). The in vitro antibacterial and antifungal activities of the isolated compounds, as well as the total methanol extract, were studied against different micro-organisms; compound 1 displayed reasonable antifungal activities towards the tested fungi.

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