Effect of coffee consumption on liver enzymes (alanine transaminase, aspartate transaminase and alkaline phosphatase), total and conjugated bilirubin levels among students in Nnewi

Background: Coffee has a number of bioactive compounds that have beneficial effects on human health in general and more importantly is the fact that coffee consumption has positive impact on the liver function. The present study investigated the effect of coffee consumption on liver enzymes: alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), total bilirubin (TB) and conjugated bilirubin (CB) levels among student in Nnewi.Methods: A total of twenty-five (25) apparently healthy participants aged between 18 and 30 years which were randomly recruited from College of Health Sciences’ students to serve as both test and control group. Each participant was advised to abstain from coffee and similar beverages for a period of three weeks prior to the commencement of the study. Subsequently, in addition to their normal diet, each of the subjects was given 2 gm of coffee dissolved in 150 ml of hot water in which 50 mg of non-dairy creamer was added before breakfast daily for 28 consecutive days. 6 ml each of baseline and test samples (after an overnight fast) were collected at day 0 and 29 respectively from each participant. Liver enzymes such as ALT, AST, and ALP activity as well as TB and CB levels was determined using standard laboratory methods.Results: This showed significant reductions in the mean serum AST and ALT activity, TB and unconjugated bilirubin (UB) levels with no significant alterations in the mean ALP activity and CB level in participants studied after short-term coffee consumption.Conclusions: This study revealed the hepatoprotective effect of coffee intake at short term basis.

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Changes in Liver Function Enzymes in Plasmodium falciparum Infected Malaria Patients in Ajeromi General Hospital, Lagos, Nigeria

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2020/v6i330120 ◽

2020 ◽

pp. 41-49

Author(s):

U. O. Ozojiofor ◽

O. O. Bankole ◽

I. O. Adedeji ◽

K. C. Onuh

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Age Groups ◽

Alanine Transaminase ◽

Age Group ◽

Aspartate Transaminase ◽

Control Subjects ◽

High Parasitaemia ◽

The Mean ◽

Group 10

Objective: This study is to determine the changes in liver function enzymes in P. falciparum-infected patients in Ajeromi ifelodun area of Lagos, Nigeria by examining changes in some liver enzymes: Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Alkaline phosphatase (ALP) obtained from newly diagnosed cases of malarial infection yet to be treated. Study Design: Seventy (70) human subjects comprising of 50 P. falciparum malarial infected and 20 non-infected (control) subjects between 10-60 years were selected for this study. Malaria positive subjects were divided into three groups based on the number of parasite per μl. Those that had parasitaemia below 10,000 parasites per μl were considered a mild infection, those that had parasitaemia above 10,000 parasites per μl were considered severe infections and those with parasitaemia below 1,000 parasites per μl were considered a low infection. RDT test and microscopy was carried out to ascertain the presence of P.falciparum. They were grouped based on age group, sex and level of parasitaemia. Materials and Methods: Blood samples were collected for the determination of P. falciparum, level of parasitaemia and liver function enzymes Aspartate Transaminase (AST), Alanine Transaminase (ALT) and Alkaline Phosphatase (ALP). P. falciparum was determined by Rapid Diagnostic Test (RDT) and microscopy. Results: There was a significant increase (P < 0.001) in the mean level of serum Aspartate Transaminase (AST), Alanine Transaminase (ALT) and a significant decrease in the mean level of alkaline phosphatase (ALP) in the P. falciparum-infected patients compared to the control subjects. AST level was found to be significantly higher (P<0.001) among age group (51- above) in the P. falciparum patients and significantly lower among age group (10-20) in the control subjects when compared to other age groups. ALT level was significantly higher (P < 0.001) among age group (51- above) in the P. falciparum patients and significantly lower among age group (41-50) in the control subjects when compared to other age groups. ALP level was significantly higher (P < 0.001) among age group (41-50) in the P. falciparum patients and significantly lower among age group (10-20) in the control subjects when compared to other age groups. AST and ALT were found to be significantly higher (P < 0.001) in the high parasitaemia group when compared to the low and moderate parasitaemia group and they were also significantly higher in the moderate parasitaemia group than in the low parasitaemia group.ALP was significantly higher (P < 0.001) in the moderate parasitaemia group when compared to the low and high parasitaemia group and they were only marginally lower in the high than in the low parasitaemia group. Conclusion: There was an elevated level of liver function enzymes in the P. falciparum patients than in the control subjects. This study shows that high parasitaemic patients are at greater risk of hepatic damage than the low parasitaemic group, hence early diagnosis and treatment of malaria are highly encouraged.

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Comparison of deferasirox and deferoxamine effect on liver enzyme activities and ferritin level in patients with beta-thalassemia

Zanco Journal of Medical Sciences ◽

10.15218/zjms.2020.042 ◽

2020 ◽

Vol 24 (3) ◽

pp. 354-359

Author(s):

Rasul Ali ◽

Dler Ali ◽

Zhyan Ahmed ◽

Amal Ahmed ◽

Dilvin Hassen ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Liver Enzyme ◽

Serum Ferritin ◽

Enzyme Activities ◽

Serum Ferritin Level ◽

Liver Enzymes ◽

Ferritin Level ◽

Alanine Transaminase ◽

Aspartate Transaminase ◽

Blood Disorder

Background and objective: Patients on blood transfusion may develop complications related to iron overload and the effects of chelating agents (drugs), which adversely affect the liver in thalassemia, which is a genetic blood disorder of hemoglobin synthesis that causes severe anemia. This study aimed to assess the effect of deferasirox and deferoxamine drugs on liver enzyme activities (aspartate transaminase, alanine transaminase, and alkaline phosphatase), and serum ferritin level in β_thalassemic patients. Methods: This study was carried out in Erbil city from October 2017 to February 2018. Blood specimens were collected in Hawler thalassemia center and Raparin hospital. The samples included 105 individuals; 70 β-thalassemic patients (35 treated by deferasirox as group A and 35 treated by deferoxamine as group B), and 35 healthy individuals (control group C). Results: The serum aspartate transaminase and alkaline phosphatase activities in β-thalassemic patients were higher than that of controls. The serum alanine transaminase activity in the patients' groups showed non-significant elevation than that of controls. The serum ferritin level in the patients was significantly higher than that of controls. Conclusion: Our finding showed that both deferasirox and deferoxamine drugs affect liver enzymes and increase ferritin level. Keywords: β-thalassemia; Deferasirox; Deferoxamine; Liver enzymes; Ferritin.

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Residual Alkaline Phosphatase Activity in Milks Subjected to Various Time-Temperature Treatments

Journal of Food Protection ◽

10.4315/0362-028x-60.5.525 ◽

1997 ◽

Vol 60 (5) ◽

pp. 525-530 ◽

Cited By ~ 22

Author(s):

C. J. PAINTER ◽

R. L. BRADLEY

Keyword(s):

Alkaline Phosphatase ◽

Activity Levels ◽

Milk Product ◽

Milk Products ◽

Alp Activity ◽

High Temperature Short Time ◽

Thermally Processed ◽

Long Time ◽

The Mean ◽

Fluid Milk

Milk is routinely tested for proper pasteurization. The Scharer and Fluorophos methods, among others, test for residual alkaline phosphatase (ALP) activity to assure proper pasteurization. Until recently there were no tests available to accurately detect residual ALP activity levels below the U.S. legal limit of 1 μg of phenol or 350 mU of ALP per liter of milk. The new Fluorophos method can detect accurately residual ALP activity levels as low as 10 mU/liter. The Fluorophos method was used to investigate residual ALP activity levels in several fluid milk products. The milk products were thermally processed under various time and temperature protocols below, at, and above current U.S. Food and Drug Administration-mandated heat treatments for fluid milk and milk products. The data established values for residual ALP activity in milks pasteurized under high-temperature short-time (HTST) and low-temperature long-time (LTLT) treatments. The mean ALP activities for whole, 2% lowfat, 1% lowfat, skim, half and half, and chocolate-flavored milks thermally processed at the legal minimum HTST pasteurization treatment are 169.7 ± 12.3, 145.2 ± 9.3, 98.6 ± 8.9, 72.5 ± 4.2, 38.4 ± 4.6 and 157.3 ± 6.5 mU/liter, respectively. The mean ALP activities generated at the legal minimum LTLT pasteurization treatment are 81.8 ± 4.8, 66.4 ± 5.9, 56.4 ± 2.1, 39.1 ± 3.9, 35.0 ± 1.2 and 91.3 ± 7.7 mU/liter, respectively. The values for all milks pasteurized at the legal minimum heat treatment were significantly below the current legal cutoff for residual ALP activity of 350 mU/liter of milk or milk product.

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Assessment of liver enzymes in saliva and serum of Iraqi patients with chronic periodontitis disease

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2020.14.1.584 ◽

2020 ◽

Vol 14 (1) ◽

pp. 20-24

Author(s):

Hussein SH. Ridha ◽

Zahraa H.M. Kadri

Keyword(s):

Alkaline Phosphatase ◽

Chronic Periodontitis ◽

Alanine Aminotransferase ◽

Liver Enzymes ◽

Serum Levels ◽

Aspartate Transaminase ◽

Gamma Glutamyl Transpeptidase ◽

Serum Samples ◽

Gamma Glutamyl ◽

Glutamyl Transpeptidase

Objective: The present study aimed to assess of four liver enzymes, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT). Material and Methods: Based on periodontal clinical parameters, sixty four patients with chronic periodontitis (CP) and twenty four controls were enrolled in the study. Saliva and serum samples were collected and Automated Chemistry Analyzer AU 480 was employed to assess levels of enzymes. Results: Compared to healthy controls, the levels of the four enzymes were significant increased in serum of patients, especially in the severe group while in the saliva a significant increase observed only in the level of AST. Moreover, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), Alkaline Phosphatase (ALP) and Gamma-Glutamyl Transpeptidase (GGT) the levels of these enzymes in serum were significantly higher than those in saliva. Conclusion: ALT, AST, ALP and GGT serum levels are suggested to be important indicators for disease progression as well as predict the liver health.

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The effect of obesity and Insulin Resistance on Liver Enzymes in Type2 Diabetes Mellitus

Baghdad Science Journal ◽

10.21123/bsj.12.3.536-545 ◽

2015 ◽

Vol 12 (3) ◽

pp. 536-545

Author(s):

Baghdad Science Journal

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Alkaline Phosphatase ◽

Negative Correlation ◽

Liver Enzymes ◽

Alanine Transaminase ◽

Obese Group ◽

Control Group ◽

Diabetic Patients ◽

And Control

Diabetes mellitus (DM) has been defined as a clinical syndrome that is characterized by abnormal carbohydrate metabolism. The chronic hyperglycemia of diabetes is associated with long term damage, dysfunction, and failure of different organs, especially the liver .This study was conducted to assess the effect obesity and insulin resistance on liver enzymes in diabetic Iraqi patients.A comparative study of (90) Iraqi adults divided to three subgroup(30) obese ,(30) nonobese diabetic patients and(30)person had used as control. The analysis included Liver enzyme ALP,ALT,AST,GGT ,Fasting Plasma Glucose (FBG) , Lipid Profile , Hemoglobin A1C , insulin and homeostasis model assessment of insulin resistance (HOMA IR) were measured. Subjects were excluded from this study if they had liver disease, alcohol intake, medications for lowering lipid, insulin treatment, pregnant women and women taking contraceptive pills . The study shows significantly higher of liver enzymes level ( gamma glutamyl transpeptidase (GGT), alkaline phosphatase, Aspartate Amino Transferase , Alanine Transaminase) in obese diabetic patients compared with non-obese diabetic patients and control subject and HOMA IR showed significantly higher in obese diabetic patients compared with non-obese with diabetic patients and control (P < 0.05). The lipids level showed significantly higher in obese diabetic patients compared with non-obese diabetic patients and control.The HbA1c level showed higher significantly in obese diabetic patients compared with control and ther is a posative correlation between insulin and HOMA IR , ALP in obeses diabetic patients while there was negative correlation between ALT and cholesterol in obese group and with HbA1c in control group. The liver enzymes level of(alkaline phosphatase, alanine transaminase, aspartate transaminase gama glutaminase transferase ) is significantly higher in obese diabetic patients than non –obese diabetic patients and control group , also There was posative correlation between ALP and HOMA IR while there was negative correlation between ALT and cholesterol in obese group and with HbA1c in control group .

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Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2020.8.2.0422 ◽

2020 ◽

Vol 8 (2) ◽

pp. 205-212

Author(s):

Tunji Akande ◽

Timothy Olugbenga Ogundeko ◽

Solomon Oyewale Opajobi ◽

Samaila Garba Yaura ◽

Isiaiah Iorkyase Idyu ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Aqueous Extract ◽

Azadirachta Indica ◽

Total Bilirubin ◽

Alanine Transaminase ◽

The Body ◽

High Rate ◽

Care Needs ◽

Conjugated Bilirubin

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

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Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2020.5.2.0422 ◽

2020 ◽

Vol 8 (2) ◽

pp. 205-212

Author(s):

Tunji Akande ◽

Timothy Olugbenga Ogundeko ◽

Solomon Oyewale Opajobi ◽

Samaila Garba Yaura ◽

Isiaiah Iorkyase Idyu ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Aqueous Extract ◽

Azadirachta Indica ◽

Total Bilirubin ◽

Alanine Transaminase ◽

The Body ◽

High Rate ◽

Care Needs ◽

Conjugated Bilirubin

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Changes in Biochemical Parameters in Human immunodeficiency virus Infected Patients: A Pre-Treatment Study

Journal of BioMedical Research and Clinical Practice ◽

10.46912/jbrcp.42 ◽

2018 ◽

Vol 1 (1) ◽

pp. 95-101

Author(s):

D O Ochalefu ◽

H A Abu ◽

E OO Amali ◽

A S Agada ◽

I E Alonyenu

Keyword(s):

Human Immunodeficiency Virus ◽

Alkaline Phosphatase ◽

Biochemical Parameters ◽

Density Lipoprotein ◽

The Body ◽

Mean Values ◽

Immunodeficiency Virus ◽

The Mean ◽

Pre Treatment ◽

Conjugated Bilirubin

In recent times there have been advances in the management of Human Immunodeficiency Virus infection. However, the disease still poses threat to biochemical parameters of the body. This study was carried out to investigate the biochemical parameters in Human Immunodeficiency Virus infected patients who were yet to start anti-Retroviral drugs regimen. This study was carried out for a period of six weeks using structural questionnaires. A total of one hundred participants were used for the study out of which fifty were Human Immunodeficiency Virus infected while the remaining fifty were Human Immunodeficiency Virus negative, who served as control. There were significant differences (p<0.05) in the values of Total Protein, Albumin, Alkaline Phosphatase, Aspartate aminotransferase, Alanine aminotransferase, Total Cholesterol, High Density Lipoprotein Cholesterol, Triacylglycerol, Calcium and Sodium of Human Immunodeficiency Virus positive patients when compared with the control. However, there were no significant differences (p>0.05) between the mean values of the conjugated Bilirubin, Urea, Creatinine, Potassium, Bicarbonate and Chloride of the infected patients when compared with those of the control. The findings in this study showed significant changes in biochemical parameters in Human Immunodeficiency Virus infected subjects.

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A 32-year-old obese Male with Prolonged Increased Transaminase Level Associated with NASH after HBV Hepatitis

Journal of Medicine ◽

10.3329/jom.v11i1.4283 ◽

1970 ◽

Vol 11 (1) ◽

pp. 90-93

Author(s):

Ahmedul Kabir ◽

Jayanta Banik ◽

Robed Amin ◽

Faizul Islam Chowdhury

Keyword(s):

Chronic Hepatitis ◽

Liver Enzymes ◽

Transaminase Level ◽

Elevated Serum ◽

Hbv Dna ◽

Alanine Transaminase ◽

Aspartate Transaminase ◽

Alcoholic Fatty Liver ◽

Alcoholic Steatohepatitis ◽

Obese Male

Nonalcoholic steatohepatitis (NASH) is an underdiagnosed liver disease and is the main cause of persistent elevated serum liver enzymes among the general population. Here, we report the case of a 32-year-old obese man who was diagnosed as a case of chronic hepatitis caused by HBV infection. He was treated with Lamivudine for two years. Though his HBV DNA load became negative, he had persistently elevated ALT and AST for two years. Then a liver biopsy was planned for greater diagnostic and prognostic certainty, as the patient was obese, and aspartate transaminase to alanine transaminase ratio was greater than one. The histopathology showed steatosis, ballooning degeneration, microinflammation and fibrosis compatible with non-alcoholic steatohepatitis. We present the case as we think that clinicians should be aware of the possibility, that hepatitis B viral hepatitis with prolonged elevated serum liver enzymes might be associated with non-alcoholic steatohepatitis. Keywords: Non-alcoholic fatty liver, steatohepatitis DOI:10.3329/jom.v11i1.4283 J Medicine 2010: 11: 90-93

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