scholarly journals The potential protective role of coenzyme q10 on the cyclophosphamide-induced lung toxicity in adult male albino rats: a histological and ultrastructural study

2018 ◽  
Vol 4 (9) ◽  
pp. 225 ◽  
Author(s):  
Nouran K. Olama ◽  
Medhat Taha ◽  
Hagar Y. Rady
Keyword(s):  
Adult Male ◽  
Coenzyme Q10 ◽  
Histopathological Examination ◽  
Treated Group ◽  
Collagen Fibrils ◽  
Lung Toxicity ◽  
Albino Rats ◽  
Blood Capillaries ◽  
Ultrathin Sections ◽  
Alveolar Septa

<p class="abstract"><strong>Background:</strong> Cyclophosphamide is anticancer and immunosuppressant agent used to treat malignant and autoimmune diseases. Its long-term use causes side effects, as infertility and pulmonary toxicity. Coenzyme Q10; the only synthesized antioxidant in human body, acts as powerful antioxidant, scavenging free radicals, and inhibiting lipid peroxidation. Aim of present study was to examine effect of coenzyme Q10 on blood biochemical profiles, histopathological changes in lungs of adult rats exposed to cyclophosphamide-induced toxicity.</p><p class="abstract"><strong>Methods:</strong> 36 adult male albino rats divided into four groups; control and three experimental each having 9 rats. First experimental group received coenzyme Q10, second received cyclophosphamide while third group received coenzyme Q10 along with cyclophosphamide. Experiment lasted for 7 days. On 8th day, animals were sacrificed by decapitation. Lung tissue samples were collected for histopathological examination. SOD (superoxide dismutase) and MDA (malondialdehyde) levels were determined and used for statistical analysis.  </p><p class="abstract"><strong>Results:</strong> In coenzyme Q10 treated group, H&amp;E stained sections revealed normal respiratory alveoli. Ultrathin sections revealed normal alveolar septa, pneumocyte and blood capillaries contain erythrocytes. In cyclophosphamide treated group, H&amp;E stained sections revealed peribronchial and interstitial fibrosis. Ultrathin sections revealed alveoli having apparent free lumen with extravasated erythrocytes. Alveolar septa revealed collagen fibrils deposits, and proliferated fibroblasts. In combined coenzyme Q10 and cyclophosphamide treated group, H&amp;E stained sections revealed marked decrease of inter-alveolar tissue thickening. Ultrathin sections revealed destructed alveolar septa with dissociated remnants of collagen fibrils. Blood capillaries appeared wide, containing monocytes and erythrocytes.</p><p class="abstract"><strong>Conclusions:</strong> Administration of coenzyme Q10 with cyclophosphamide is advised to alleviate cyclophosphamide-induced lung toxicity.</p>

scholarly journals Role of erythropoietin in methotrexate-induced nephrotoxicity in adult male albino rats

2018 ◽  
Vol 7 (2) ◽  
pp. 156-163 ◽  
Author(s):  
Ahmed Hassan Al-Rashidy ◽  
Rasha Rabea Salem ◽  
Amal Abdelrasool Alhosary ◽  
Mohamed Hassan Wahdan ◽  
Gamal Mohamed Elnemr ◽  
...  
Keyword(s):  
Adult Male ◽  
Histopathological Examination ◽  
Treated Group ◽  
Albino Rats ◽  
Human Erythropoietin ◽  
Renal Changes ◽  
Group 3 ◽  
Group 2 ◽  
And Control

Introduction: Methotrexate (MTX) could provoke a renal dysfunction. However, beneficial extra-hematopoietic effect of erythropoietin might guard against MTX-induced nephrotoxicity. Objectives: Determination of renoprotective erythropoietin’s role against MTX-induced nephrotoxicity through elucidating its renofunctional and renomorphological effects in adult male albino rats. Materials and Methods: The study was performed on 60 adult male Albino rats, equally divided into three groups; group 1 (control): treated with intraperitoneal injections of normal saline at a dosage of 0.5 mg/kg BW twice weekly for 9 weeks. group 2: injected with MTX hydrate intraperitoneal twice weekly at a dosage of 0.5 mg/kg BW for 9 weeks; and group 3: intraperitoneal injected with MTX hydrate in a similar dosage and duration like group 2 concomitant with subcutaneous injection of 100 IU/kg recombinant human erythropoietin once weekly for 9 weeks. At the study end, serum urea and creatinine together with albuminuria were measured, rats were sacrificed and renal sections were prepared for histopathological examination. Results: Significantly increased values of renal function analyzed substances with deteriorated histopathological renal changes were detected in the MTX-treated group compared to either the control or to the MTX and erythropoietin co-treated group. The later displayed statistically significant decreased levels of the substances accompanied by remarkably ameliorated microscopic renal changes. Additionally, insignificant statistical biochemical and morphological renal differences were noticed between the third and control groups. Conclusion: This study concluded valuable and efficient defense against MTX-induced nephrotoxicity in adult male Albino rats when co-treated with erythropoietin.

scholarly journals THE ROLE OF ASCORBIC ACID IN ZINC OXIDE NANO-PARTICLES INDUCED LUNG TOXICITY IN ADULT MALE ALBINO RATS

2019 ◽  
Vol 16 (Supplement) ◽  
pp. 35-55
Author(s):  
Madiha W. Mohamed ◽  
Yara M. El-fakharany ◽  
Nourhan M. Hassan ◽  
Heba M. Elsayed
Keyword(s):  
Ascorbic Acid ◽  
Zinc Oxide ◽  
Adult Male ◽  
Lung Toxicity ◽  
Nano Particles ◽  
Albino Rats

scholarly journals THE EFFECT OF COENZYME Q10 AND/OR SILYMARIN ON RENALASE GENE EXPRESSION OF CARDIORENAL SYNDROME IN ADULT MALE ALBINO RATS

2018 ◽  
Vol 47 (3) ◽  
pp. 647-660
Author(s):  
Gehan A. Youssef ◽  
Mona G. Al Anany ◽  
Ghada M. M. Salah El-din ◽  
Sara N. M. Mousa
Keyword(s):  
Gene Expression ◽  
Adult Male ◽  
Coenzyme Q10 ◽  
Cardiorenal Syndrome ◽  
Albino Rats

The possible protective role of Vitamin C versus Mesna against effect of Cyclophosphamide on the Urinary Bladder of Adult Male Albino Rats (A Histological and Immunohistochemical Study)

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Walaa Adel Abd El Moez Ahmed ◽  
Seham Hassan Refaat ◽  
Hany Waheeb Abd El-Malak ◽  
Asmaa Ibrahim Ahmed ◽  
Ashraf Mohammed Mostafa Sadek
Keyword(s):  
Urinary Bladder ◽  
Vitamin C ◽  
Lamina Propria ◽  
Adult Male ◽  
Treated Group ◽  
Albino Rats ◽  
Group V ◽  
Minimal Improvement ◽  
Anti Oxidant ◽  
Recovery Group

Abstract Background Cyclophosphamide (CYP) is considered one of the most successful chemotherapeutic drugs involved in anticancer regimens. However, it has multiple side effects. Mesna has an antiinflammatory effect and usually used in the treatment of cystitis. Vitamin C is a water-soluble vitamin which has a potent anti –oxidant effect that might protect cells against the oxidative damage caused by cyclophosphamide. Aim of the study The aim of the present study was comparing between the possible protective effect of vitamin C versus mesna and their combined therapy against the histological and immunohistochemical changes induced by cyclophosphamide on the urinary bladder of adult male albino rats. Material and methods Thirty adult male albino rats were divided into 5 groups, 6 rats each; (control(?), CYP-treated group (Пa), recovery group(Пb), mesna-treated group(???), vitamin C- treated group(?V) and the combined group (V). Histological examination of the H&Eand toluidine blue stained sections was done by light microscopy to assess the changes in the architecture of the urinary bladder. Avidin Biotin staining was performed for demonstration of iNOS immunoreactivity and histomorphometric analysis was done. Results Examination of H&E stained sections of cyclophosphamide- treated group (Пa) showed variable degrees of urothelial affection. Wide areas of urothelial cell degeneration with evident basal cytoplasmic vacoulatins, surface erosions and sloughed urothelial debris. Other Areas showed surface ulceration, completely denuded urothelium or the presence of multiple cysts replacing the urothelium and resting on the basement membrane. Semithin sections showed that the cytoplasmic microvesicles of umbrella cells were hardly detected. The Avidin Bioton stained sections showed intense positive immune reaction to iNOS in all layers of the urothelium. Scanning electron microscopy showed loss of the normal polygonal shape of the superficial epithelial cells, erosions, or deep ulcerations. Moreover, examination of the lamina propria by light microscopy showed multiple mononuclear inflammatory cells were detected, mast cells were seen in the lamina propria and some of them were invading the basement membrane of the urothelium. Dilated blood vessels and wide areas of extravasted blood (hemorrhage) were also observed. In addition, multiple epithelial cell nests of irregular shapes and sizes were deeply located in the lamina propria and exhibited pale esinophilic colloid discharge in their lumen. Scanning electron microscopy showed dense deposition of collagen fibers in both superficial and deep fibers of the lamina propria. Minimal improvement was observed in the recovery group (subgroup Пb). Mild to moderate improvement of the previous findings of CYP treated group was observed with each of mesna and vitamin C. Combined treatment of CYP with both of mesna and vitamin C induced apparent restoration of almost of the normal architecture of the urinary bladder. Conclusion CYP consumption developed morphologic and morphometric changes in the urinary bladder. The recovery group showed minimal improvement of the bladder architecture and increasing the period of recovery might produce better results. Each of vitamin C and mesna- treated groups induced mild to moderate improvement on the bladder architecture but treatment with combination of both of them offered remarkable improvement. Combined mesna and vitamin C induced significant protection via their combined anti-inflammatory and anti-oxidant proprieties.

scholarly journals HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL INDUCED TOXICITY IN MALE ALBINO RATS

2020 ◽  
pp. 47-51
Author(s):  
MEENATCHI SUNDARAM ANGAPPAN
Keyword(s):  
Antioxidant Enzymes ◽  
Histopathological Examination ◽  
Treated Group ◽  
Hepatoprotective Effect ◽  
Hepatic Regeneration ◽  
Albino Rats ◽  
Ether Anesthesia ◽  
Polyherbal Formulation ◽  
Group I ◽  
Ameliorative Effect

Objective: The aim of this current study is to investigate the hepatoprotective efficacy of N-Miracle (a polyherbal formulation) against ethanol-induced toxicity in male albino rats. Methods: Male Wistar albino rats weighing 150-200 g were used for the study. A total of 30 male albino rats were selected, divided into five groups. Ethanol-induced liver damage was done on Group III, IV, and V. Group I and Group II served as a normal and drug (N-Miracle) control. After the treatment period, the rats were anaesthetized by light ether anesthesia in a lethal chamber. Hepatic biomarkers, antioxidant enzymes, histopathological examination are carried out to document the hepatoprotective effect of N-Miracle (Polyherbal formulation). Results: The results of the present study demonstrated a significant (p<0.05) increase in the levels of Aspartate Aminotransaminase (AST), Alanine Aminotransaminase (ALT) and Alkaline Phosphatase (ALP) in ethanol-induced rats as compared to normal and drug control Groups. The level of total protein and albumin were significantly (p<0.05) decreased in ethanol-treated rats. The toxic impact of ethanol was found to be restored in rats treated with N-Miracle (Polyherbal formulation). The present study also exhibited the enzymatic antioxidant efficacy of N-Miracle (Polyherbal formulation) against ethanol-induced toxicity in rats by increasing the antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and decreasing the activity of Glutathione-S-transferase in the liver. The findings are also correlated with histopathological examination of N-Miracle treated group, which shows hepatic regeneration and decrease in degradation of hepatocytes. Conclusion: This study could provide a possible explanation to hepatotoxicity resulting from exposure to ethanol. The findings of the present study revealed the ameliorative effect of N-Miracle (Polyherbal formulation) against ethanol-induced hepatotoxicity by improving the liver function, increasing the levels of antioxidant enzymes and restoring the morphological features of the liver.

scholarly journals Hyperbaric Oxygen Therapy Ameliorates the Harmful Effects of Dexamethasone Induced Diabetes on Liver and Pancreas of Adult Male Albino Rats

2020 ◽  
pp. 40-54
Author(s):  
Nehal Farid El-Helbawy ◽  
Alhaz Abd Al-Hai Abd Al-Salam ◽  
Manal El-Sayed El-Sawaf ◽  
Safwat Abd El-Aziz El-Deeb
Keyword(s):  
Blood Glucose ◽  
Hyperbaric Oxygen ◽  
Adult Male ◽  
Hyperbaric Oxygen Therapy ◽  
Oxygen Therapy ◽  
Treated Group ◽  
Group Iv ◽  
Albino Rats ◽  
Group Iii ◽  
Liver And Pancreas

Aims: This study was done to clarify the effects of hyperbaric oxygen therapy on the harmful changes of liver and pancreas of adult male albino rats after dexamethasone induced diabetes. Place of Study: Faculty of medicine, Tanta University, Egypt. Methodology: Thirty two adult male albino rats were divided into; group I (control), group II (Hyperbaric oxygen treated), Group III (induced diabetes - non treated group) and Group IV (induced diabetes + Hyperbaric oxygen treated group). Induction of diabetes was done by dexamethasone injection for ten days. Hyperbaric oxygen was given once per day for 5 sessions in group II and IV. The rats of all groups were sacrificed after the 20th day. Specimens of liver and pancreas were subjected to microscopic examination. Results: Rats from group III showed a highly significant increase of blood glucose compared to the controls. Treated rats in group IV showed highly significant decrease in blood glucose compared to group III. Hepatic steatosis and histopathological changes of pancreatic acini and islets of Langerhans were noticed in untreated diabetic rats. Group IV after Hyperbaric oxygen therapy revealed partial improvement of histological and ultrastructural effects of diabetes on the liver and pancreas. Conclusions: HBOT is partially effective in reducing blood glucose level and improving the hazardous effect of untreated diabetes on the histological and ultrastructural architecture of the liver and pancreas of male albino rats.

scholarly journals Effect of Flax Seed Oil on Acute Carbon Tetrachloride-induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Gorkem Ekebas ◽  
Ayhan Atasever ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Total Cholesterol ◽  
Total Protein ◽  
Caspase 3 ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Treated Group ◽  
Flaxseed Oil ◽  
Albino Rats ◽  
Intraperitoneal Dose

Aims: The present study was designed to evaluate the hepatoprotective activity of flaxseed oil (FSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats by measurement of caspase 3, 8 and 9 activities in cellular apoptosis, ALT activities, triglyceride, total protein, total cholesterol and liver MDA levels. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between June 2017 and July 2018. Methodology: In this study 32 male Wistar albino rats were divided into four groups of 8 animals in each. The first group was identified as the control and received an intraperitoneal 0.9% NaCl and the second group was given per os at dose of 4 ml/kg FSO for 4 weeks. The third group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week. The fourth group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week and simultaneously 4 ml/kg FSO by gavage for 4 weeks. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, lymphocytes rich mononuclear cell infiltration in portal area and parenchyma. The flaxseed oil application did not ameliorate the histological changes induced by CCl4, however reduced the activity of caspase 3, 8 and 9 by a limited number. CCl4 administration produced significantly elevated levels of serum ALT activity, total cholesterol, triglyceride and liver MDA levels, and these increases were not normalized with FSO treatment. In addition, decreased serum total protein levels in CCl4 treated group were ameliorated by FSO application. Conclusion: The results indicate that the antioxidant properties of FSO do not have an ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. In addition, it was concluded that duration‐dependent further research results are needed to determine the effects of flaxseed oil in high doses that can give the best results without side effects.

scholarly journals Impact of Human Umbilical Cord Blood Mononuclear Cells on Gentamicin-Induced Renal Injury and Genotoxicity in Rats

2021 ◽  
Vol 8 ◽  
Author(s):  
Ali H. Abu Almaaty ◽  
Reham A. Elmasry ◽  
Mayada S. Farrag ◽  
Fayez Althobaiti ◽  
Adil Aldhahrani ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Histopathological Examination ◽  
Kidney Injury ◽  
Treated Group ◽  
Albino Rats ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Gentamicin Treatment ◽  
Healthy Control ◽  
Regenerative Property

Background: Acute kidney injury (AKI), also known as acute renal failure (ARF), has received considerable critical attention in recent years. Gentamicin (GM) is an antibiotic whose prolonged use results in AKI with a high mortality rate.Methods: Fifty adult female albino rats weighing 150–200 g were used. The animals were divided into five groups: the first group was the normal healthy control one, the second group received only 1 × 106 HUCB mononuclear cells (MNCs)/rat by intravenous (iv) injection, the third diseased group was given GM 100 mg/kg for 10 consecutive days by intraperitoneal injections, the fourth preventive group received 1 × 106 HUCB MNCs/rat by iv injection 24 h before gentamicin treatment, and the fifth treated group received 1 × 106 HUCB MNCs/rat by iv injection 24 h after gentamicin treatment. After 1 week of treatment, blood samples were collected, and kidneys were removed for histopathological examination.Results: Rats treated with HUCB MNCs in the treated group had a significant decrease in renal damage, low levels of biomarkers for nephrotoxicities such as serum creatinine and blood urea nitrogen, and low chromosomal aberrations compared to the diseased third group. The gene expression of KIM-1 and NGAL was decreased in response to HUCB treatment.Conclusions: HUCB MNCs have a curative effect against AKI and gentamicin-induced genotoxicity owing to their regenerative property.

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