scholarly journals The study of genetic factors that determine the awned glume trait in bread wheat

2020 ◽  
Vol 24 (6) ◽  
pp. 568-574
Author(s):  
O. B. Dobrovolskaya ◽  
A. E. Dresvyannikova ◽  
E. D. Badaeva ◽  
K. I. Popova ◽  
M. Trávníčková ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Genetic Mapping ◽  
Bread Wheat ◽  
Oryza Sativa L ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Inflorescence Development ◽  
Cereal Species ◽  
Scanning Electron

Awns are bristle‐like structures, typically extending from the tip end of the lemmas in the florets of cereal species, including such economically important crops as wheat (Triticum aestivum L., T. durum Desf.), barley (Hordeum vulgare L.), rice (Oryza sativa L.), and rye (Secale cereale L.). The presence of long awns adhered at tip end of glumes is a characteristic feature of “Persian wheat” T. carthlicum Nevski spike. Glume outgrowth of T. carthlicum Nevski spike passes into a long awn, equal in length to the lemma awn. Awned glumes can be formed in T. aestivum and T. aethiopicum wheats, however, such forms are rare. Features of the awned glume development and the genetic determinants of this trait have been little studied. In this paper, we described the features of the development and inheritance of the tetra-awness (awned glume) trait of the bread wheat T. aestivum line CD 1167-8, using classical genetic analysis, molecular genetic mapping, and scanning electron microscopy. It was shown that the trait is inherited as a recessive monogenic. The gene for the awned glume trait of CD 1167-8 was mapped in the long arm of chromosome 5A, using the Illumina Infinium 15K Wheat Array (TraitGenetics GmbH), containing 15,000 SNPs associated with wheat genes. Results of allelism test and molecular-genetic mapping suggest that the gene for awned glumes in bread wheat is a recessive allele of the B1 awn suppressor. This new allele was designated the b1.ag (b1. awned glume). Analysis of the CD 1167-8 inflorescence development, using scanning electron microscopy, showed that awns had grown from the top of the lemmas and glumes simultaneously, and no differences in patterns of their development were found.

Silica deposition in the inflorescence bracts of wheat (Triticum aestivum). I. Scanning electron microscopy and light microscopy

1988 ◽  
Vol 66 (5) ◽  
pp. 829-838 ◽  
Author(s):  
M. J. Hodson ◽  
A. G. Sangster
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Triticum Aestivum ◽  
Light Microscopy ◽  
Triticum Aestivum L ◽  
The Other ◽  
Deposition Pattern ◽  
Membranous Structure ◽  
Silica Deposition ◽  
Scanning Electron

Silica deposition in the lower glume, lemma, and palea of wheat (Triticum aestivum L. cv. Highbury) was investigated using scanning electron microscopy and light microscopy. Silica was present in the outer walls of all the epidermal cells including prickles and papillae of the glume and lemma awns. The glume and the lemma were similar in epidermal silica deposition pattern, both having numerous silicified short trichomes and papillae on inner and outer surfaces. Epidermal long cells and short cells were also silicified. Macrohairs were restricted to isolated areas in these bracts, particularly on the inner surfaces just beneath the awns. The palea was a thin membranous structure differing markedly from the other two bracts. Most of the palea is pressed between the caryopsis and the next floret, and both surfaces are almost devoid of trichomes in these areas. However, at the apex and margins of the palea, macrohairs and papillae were abundant. The results are discussed with respect to possible taxonomic, anatomical, medical, and archaeological implications.

scholarly journals Architectural Development of Inflorescence in Hydrangea macrophylla cv. Hermann Dienemann

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Gilles Galopin ◽  
Sandrine Codarin ◽  
Jean-Daniel Viemont ◽  
Philippe Morel
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Inflorescence Development ◽  
Schematic Representation ◽  
Early Stages ◽  
Hydrangea Macrophylla ◽  
Floral Differentiation ◽  
Architectural Development ◽  
Scanning Electron

Architectural development of inflorescence in Hydrangea macrophylla cv. Hermann Dienemann was observed using scanning electron microscopy. The study of inflorescence morphogenesis shows that the architecture is of the dichasial type. The first two orders of branching are initiated from a dichasial branching without floral differentiation. The following orders present floral differentiation. They determine the formation of small units through the development of composite dichasium into biparous and uniparous cymes. This research makes it possible to establish a schematic representation of the first phases of inflorescence development and to define early stages of inflorescence morphogenesis.

scholarly journals Using the Floral Status of Strawberry Plants, as Determined by Stereomicroscopy and Scanning Electron Microscopy, to Survey the Phenology of Commercial Crops

1998 ◽  
Vol 123 (4) ◽  
pp. 513-517 ◽  
Author(s):  
Y. Manakasem ◽  
P.B. Goodwin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Minimum Temperature ◽  
Maximum Temperature ◽  
Flower Initiation ◽  
Fragaria Ananassa ◽  
Inflorescence Development ◽  
Field Surveys ◽  
Scanning Electron

Field surveys were conducted on cultivated strawberries (Fragaria ×ananassa Duch.) to determine the time of flower initiation and its relation to maximum and minimum temperatures and daylength. Stereomicroscopy and scanning electron microscopy (SEM) were compared. Flower initiation in `Torrey' strawberry was more dependent on minimum temperature than on daylength or maximum temperature. Flower initiation in the day-neutral `Aptos' occurred regardless of daylength or temperature during sampling. For the study of flower initiation and inflorescence development, SEM gave more detail than stereomicroscopy.

scholarly journals Flowering and Inflorescence Development of Lachenalia aloides `Pearsonii' as Influenced by Bulb Storage and Forcing Temperature

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 833B-833
Author(s):  
Mark Roh*
Keyword(s):  
Magnetic Resonance Imaging ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Low Temperatures ◽  
Imaging Techniques ◽  
Leaf Length ◽  
Resonance Imaging ◽  
Inflorescence Development ◽  
Magnetic Resonance Imaging Techniques ◽  
Scanning Electron

The effect of bulb storage and forcing temperatures on growth, flowering, and inflorescence development and the death of inflorescence (blast) of Lachenalia aloides Engl., `Pearsonii' was investigated. Following development of about 5 florets, bulbs were stored at 10, 12.5, 15, 20, and 25 °C for 15, 30, or 45 days and forced in greenhouses at 17/15 °C and 21/19 °C. Flowering was accelerated, and leaf length and floret number were reduced, when bulbs were stored at 10, 12.5, or 15 °C for 45 days compared to storing at 20 or 25 °C. Flowering was further accelerated by forcing at 17/15 °C compared to 21/19 °C. When bulbs were stored at 10, 15, 20, or 25 °C for 4 weeks and grown in greenhouses at 17/15 °C, 21/19 °C, 25/23 °C, and 29/27 °C, the incidence of inflorescence blast was increased when bulbs were stored at 10 and 15 °C and forced at 25/23 °C compared to low temperatures. Bulbs were forced in greenhouses maintained at 18/16 °C, 22/20 °C, or 26/24 °C for 12 weeks. During forcing, plants were subjected to constant or alternating forcing temperatures at 4-week intervals. Inflorescence blast occurred when the temperature was 26/24°C during the first 4 weeks after potting. Storing Lachenalia bulbs at 10&#176 to 15 °C before potting then forcing at 17/15 °C accelerated flowering and produced quality plants with short leaves and floral stems. Inflorescence development during bulb 10 °C treatment and inflorescence blast that occurred after only 3 days of 35 °C was demonstrated using scanning electron microscopy and magnetic resonance imaging techniques.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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