scholarly journals Effect of Different Preservation Time of Chilled Semen on the Fertility of Field Indigenous Ewes

2021 ◽  
Author(s):  
A. Saha ◽  
M. Asaduzzaman ◽  
S. Akter ◽  
F.Y. Bari
Keyword(s):  
Pregnancy Rate ◽  
Efficient Method ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Acceptable Level ◽  
Rapid Improvement ◽  
Normal Sperm ◽  
Fundamental Systems ◽  
Artificial Vagina

Background: Artificial insemination (AI), among all the fundamental systems of animal breeding, has proved to be the best and efficient method for the rapid improvement of livestock for maximum use of superior genetic merit of males on numerous dams. The frozen AI in sheep is, to date, not fulfilling the farmer’s need. Therefore, the present study aimed to determine the pregnancy rate in indigenous ewes with chilled semen preserved at different hours. Methods: Semen was collected from three indigenous rams using an artificial vagina and evaluated for its quality both in the fresh and chilled stage at 12 h, 24 h and 48 h before insemination. Trans-cervical insemination was performed in PGF2α synchronized ewes. Pregnancy was confirmed by ultrasonography at 50-60 days of gestation. Result: The motility, viability and normal sperm values of chilled semen decreased significantly (p less than 0.01) with increasing the duration of preservation. However, the quality of chilled semen was acceptable level for insemination. The pregnancy rate was significantly varied (p less than 0.01) and the higher pregnancy rate (64.28%) was achieved when AI was performed using semen stored at 4oC for 12 h than 24 h (58.33%) and 48 h (50%), respectively. Therefore, increased the duration of preservation time of different chilling process negatively affect the semen quality and the pregnancy rates.

scholarly journals The The Effect of Age and Breed on The Quality of Bull Semen in The Regional Artificial Insemination Centre

2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Age Groups ◽  
Bull Semen ◽  
Sperm Abnormalities ◽  
Frozen Semen ◽  
Cattle Reproduction ◽  
Effect Of Age ◽  
Artificial Vagina

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.

Quality of ram spermatozoa separated with modified swim up method

2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Fluid Medium ◽  
Normal Morphology ◽  
The Mean ◽  
Mass Activity ◽  
Fresh Semen ◽  
Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

2016 ◽  
Vol 85 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Marina Anastasia Karageorgiou ◽  
Georgios Tsousis ◽  
Constantin M. Boscos ◽  
Eleni D. Tzika ◽  
Panagiotis D. Tassis ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Medium Term ◽  
Semen Collection ◽  
Chromatin Integrity ◽  
Boar Semen ◽  
Short Time

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

2021 ◽  
Author(s):  
Larbi ALLAI ◽  
Xavier Druart ◽  
Pinar Terzioğlue ◽  
Noureddine Louanjli ◽  
Boubker Nasser ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Skim Milk ◽  
Butylated Hydroxytoluene ◽  
Emission Spectrometry ◽  
Control Group ◽  
Opuntia Ficus Indica ◽  
Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

scholarly journals Impact of the quality of life of inseminators on the results of artificial insemination programs in beef cattle

2010 ◽  
Vol 39 (7) ◽  
pp. 1457-1463
Author(s):  
Lívia dos Santos Russi ◽  
Eliane Vianna da Costa-e-Silva ◽  
Carmem Estefânia Serra Neto Zúccari ◽  
Caciliana da Silva Recalde ◽  
Norly Gomes Cardoso
Keyword(s):  
Beef Cattle ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Life Quality ◽  
Work Conditions ◽  
Pregnancy Rates ◽  
Open Questions ◽  
Timed Artificial Insemination ◽  
And Training

The objective of the present study was to evaluate the effect of factors of personal life and work conditions on the results obtained by inseminators in conventional and fixed-timed artificial insemination programs in beef cattle. Inseminators from three farms (21 in the total) were interviewed and evaluated according to the general obtained pregnancy rates. The differences among the pregnancy rates obtained in the farms, motivation and its association with the obtained pregnancy rate and the effect of each variable of the groups of needs on the pregnancy rate at first insemination were evaluated. The open questions were grouped by similarity and then analyzed by frequency of the answers. Pregnancy rates obtained by the inseminators ranged from 12 to 57%, with a mean service index of 3.10 ± 1.62 doses/pregnancy. It was also observed that the satisfaction of biological, financial, and training needs was more intimately related to the pregnancy rate than the satisfaction of the other needs, although none had shown an antagonistic relationship with it. Factors concerned to life quality and to the work can be important in determining the performance of these professionals.

scholarly journals An Evaluation of Semen Quality of Donor Bulls in the Central Artificial Insemination Station (CAIS), Kundasale, Sri Lanka

2021 ◽  
Vol 32 (4) ◽  
pp. 426
Author(s):  
W. W. Abeygunawardana ◽  
P. G. A. Pushpakumara ◽  
H. M. S. Wasana ◽  
W. M. T. D. Rathnakumara ◽  
B. Alexander
Keyword(s):  
Sri Lanka ◽  
Artificial Insemination ◽  
Semen Quality

scholarly journals Senduro Goat Semen Characteristics as A Candidate for Low Temperature Storage

2021 ◽  
Vol 328 ◽  
pp. 08010
Author(s):  
Nur Ducha ◽  
Widowati Budijastuti ◽  
Dwi Anggorowati Rahayu
Keyword(s):  
Artificial Insemination ◽  
Low Temperatures ◽  
Semen Quality ◽  
Membrane Integrity ◽  
Animal Husbandry ◽  
Service Unit ◽  
Low Temperature Storage ◽  
Semen Characteristics ◽  
Artificial Vagina ◽  
Temperature Storage

The goal of this study was to investigate the reproductive profile of male Senduro Goats as superior male candidates based on data of semen quality. The experiment was carried out on ten male Senduro Goats kept at the Singosari Animal Husbandry Service Unit in East Java, Indonesia. An artificial vagina was used to collect sperm. The macroscopic and microscopic qualities of fresh Senduro Goat sperm have been observed. Color, volume, smell, pH, and viscosity were examples of macroscopic observations. Concentration, motility, viability, and membrane integrity were examples of microscopic observations. The results of macroscopic observations showed that the average of Senduro Goat semen was milky white; with volume 1,24 ± 0,22; 6.77 ± 0.05; thick semen. The results of microscopic observations showed that there was concentration 2,5 x 109 ± 0.1; mass motility +++; individual motility 77.5% ± 2.63; viability 88.98% ± 2.07; membrane integrity 89.71 ± 1.69. Based on the results of these studies, it can be concluded that the semen of Senduro Goat has good quality so it can be processed further, namely storage at low temperatures and applied to Artificial Insemination technology.

scholarly journals Effect of adding Manganese chloride and Co-enzymes (α-lipoic acid and Q10) on post-cryopreservation semen quality characteristics of Holstein bulls

2018 ◽  
Vol 41 (2) ◽  
pp. 86-93
Author(s):  
Eidan S. M.
Keyword(s):  
Lipoic Acid ◽  
Semen Quality ◽  
Manganese Chloride ◽  
Control Group ◽  
Control Groups ◽  
Holstein Bulls ◽  
Animal Resource ◽  
Fresh Semen ◽  
Artificial Vagina

     This study was undertaken to explore the adding effect of manganese chloride (MnCl2), co-enzyme Q10 (Co-Q10) as well as α-lipoic acid to Tris extender on Freeze ability of Holstein bulls’ semen. This study was carried out at the department of artificial insemination at the Directorate of Animal Resource, Ministry of Agriculture in Abu-Ghraib, Baghdad for the duration from October\ 2013 to Jun\ 2014, including three experiments. Seven Holstein bulls of 3.5-4 years old were used in this study. Semen was collected via artificial vagina by one ejaculate/ bull/ week. The assessments were conducted for fresh semen, which was later pooled, equally divided for various treatments within each experiment, using Tris extender. In the first experiment, pooled semen was divided into three groups. First group was diluted with Tris only. Manganese chloride was added to Tris extender (0.7 mM) in the 2nd group while in 3rd group (0.9 mM) of the Manganese chloride was used. In the second experiment, semen was divided equally into three groups. The first group was considered as a control group diluted with Tris only. Co-enzyme Q10 was added to 2nd (0.2 mM) and 3rd groups (0.5 mM) treatments respectively. In the third experiment, semen was divided into three groups. The first group was diluted with Tris only (control group). While the 2nd and 3rd groups were added 0.5 and 1.0 mM α-lipoic acid respectively. The effect of these additions on Holstein bulls semen quality was studied during different periods (48 hours, one, two and three months post cryopreservation) for three experiments. The results revealed that the addition two levels of the MnCl2 (2nd and 3rd, experiment 1), Co- Q10  (2nd and 3rd, experiment 2) and α- lipoic acid (2nd and 3rd experiment 3) led to significant increases freezability as compared with control groups during all the experiment periods. In conclusion, the addition of MnCl2, Co-Q10 and α-lipoic acid led to improved post-cryopreservation semen quality of Holstein bulls. This will in turn enhance fertility rate of artificially-inseminated cows and owners economic income consequently.

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