An Evaluation of Semen Quality of Donor Bulls in the Central Artificial Insemination Station (CAIS), Kundasale, Sri Lanka

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16–18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research.

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Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

10.21203/rs.3.rs-522174/v1 ◽

2021 ◽

Author(s):

Larbi ALLAI ◽

Xavier Druart ◽

Pinar Terzioğlue ◽

Noureddine Louanjli ◽

Boubker Nasser ◽

...

Keyword(s):

Antioxidant Activity ◽

Artificial Insemination ◽

Semen Quality ◽

Skim Milk ◽

Butylated Hydroxytoluene ◽

Emission Spectrometry ◽

Control Group ◽

Opuntia Ficus Indica ◽

Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

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Influences of bull age and season on sperm motility, sperm concentration, and ejaculate volume of Ongole Grade cattle in Singosari National Artificial Insemination Center

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.45.4.261-267 ◽

2020 ◽

Vol 45 (4) ◽

pp. 261-267

Author(s):

S. Suyadi ◽

B. Purwantara ◽

A. Furqon ◽

W. A. Septian ◽

I. Novianti ◽

...

Keyword(s):

Sperm Motility ◽

Artificial Insemination ◽

Semen Quality ◽

Sperm Concentration ◽

Dry Season ◽

Production Data ◽

Wet Season

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.

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Seasonal Influence on Post Thaw Seminal Attributes and Conception Rate from Semen of Jaffarabadi Buffalo Bulls

Indian Journal of Animal Research ◽

10.18805/ijar.b-4135 ◽

2020 ◽

Author(s):

K.H. Parmar ◽

P.H. Tank ◽

F.S. Kavani

Keyword(s):

Artificial Insemination ◽

Semen Quality ◽

Summer Season ◽

Conception Rate ◽

Seasonal Influence ◽

Abnormal Sperm ◽

The Mean ◽

Live Sperm ◽

One Year

Background: The quality of the frozen thawed semen is one of the most powerful factor affecting the conception rate in a buffaloes. The season may have its effect on post thaw seminal attributes and fertility. Therefore, current study was aimed to investigate the effect of winter and summer seasons on post thaw seminal attributes and conception rate from semen of Jaffarabadi buffalo bulls.Methods: The study was conducted on four Jaffarabadi breeding bulls, 5-6 years old, to know the post-thaw semen characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. Result: The mean percentages of post-thaw motility, live sperm, abnormal sperm, HOST reactive sperm, acrosomal integrity and first AI conception rate for semen cryopreserved during winter were 58.85±0.56, 67.06±0.77, 8.62±0.43, 58.52±0.67, 75.22±0.27 and 45.75±0.21, respectively, whereas, corresponding values for semen cryopreserved during summer were 56.35±0.43, 65.62±0.76, 9.08±0.51, 55.43±0.60, 74.29±0.22 and 43.50±0.11. The semen quality of post thawed samples were observed better during winter compared to summer season for artificial insemination with satisfactory first AI conception rate.

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Effect of Different Preservation Time of Chilled Semen on the Fertility of Field Indigenous Ewes

Agricultural Science Digest - A Research Journal ◽

10.18805/ag.d-330 ◽

2021 ◽

Author(s):

A. Saha ◽

M. Asaduzzaman ◽

S. Akter ◽

F.Y. Bari

Keyword(s):

Pregnancy Rate ◽

Efficient Method ◽

Artificial Insemination ◽

Semen Quality ◽

Acceptable Level ◽

Rapid Improvement ◽

Normal Sperm ◽

Fundamental Systems ◽

Artificial Vagina

Background: Artificial insemination (AI), among all the fundamental systems of animal breeding, has proved to be the best and efficient method for the rapid improvement of livestock for maximum use of superior genetic merit of males on numerous dams. The frozen AI in sheep is, to date, not fulfilling the farmer’s need. Therefore, the present study aimed to determine the pregnancy rate in indigenous ewes with chilled semen preserved at different hours. Methods: Semen was collected from three indigenous rams using an artificial vagina and evaluated for its quality both in the fresh and chilled stage at 12 h, 24 h and 48 h before insemination. Trans-cervical insemination was performed in PGF2α synchronized ewes. Pregnancy was confirmed by ultrasonography at 50-60 days of gestation. Result: The motility, viability and normal sperm values of chilled semen decreased significantly (p less than 0.01) with increasing the duration of preservation. However, the quality of chilled semen was acceptable level for insemination. The pregnancy rate was significantly varied (p less than 0.01) and the higher pregnancy rate (64.28%) was achieved when AI was performed using semen stored at 4oC for 12 h than 24 h (58.33%) and 48 h (50%), respectively. Therefore, increased the duration of preservation time of different chilling process negatively affect the semen quality and the pregnancy rates.

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Factors Affecting Fresh Semen Quality in Pasundan Cattle at UPTD BPPIBTSP Ciamis

Journal of Applied Veterinary Science And Technology ◽

10.20473/javest.v2.i2.2021.37-42 ◽

2021 ◽

Vol 2 (2) ◽

pp. 37

Author(s):

Oky Susandani ◽

Tri Wahyu Suprayogi ◽

Ratna Damayanti ◽

Anwar Ma'ruf

Keyword(s):

Body Weight ◽

Beef Cattle ◽

Artificial Insemination ◽

Semen Quality ◽

Factors Affecting ◽

Factors Influencing ◽

Determining Factors ◽

Semen Storage ◽

Fresh Semen

Background: Pasundan cattle are local cattle native to Indonesia. One way to conserve beef cattle genetics is to use Artificial Insemination technology. The success of Artificial Insemination can be influenced by the quality of semen. Purpose: To determine factors affecting fresh semen quality in Pasundan cattle at UPTD BPPIBTSP Ciamis. Methods: The data were obtained through observations on seven Pasundan bulls in March 2021 towards fresh semen quality and some factors influencing it. The Pasundan bulls observed were seven productive males. Results: The fresh semen quality of Pasundan cattle, such as volume, color, and pH, showed good result,s but the average consistency and concentration of spermatozoa were still below the standard. The factors that can affect the fresh semen quality are the breed of beef cattle, age, body weight, feed, season, exercise, and frequency of semen storage. Conclusion: The determining factors that can cause the consistency and concentration of Pasundan cattle’s spermatozoa at UPTD BPPIBTSP Ciamis are feed and season.

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Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program

Veterinary World ◽

10.14202/vetworld.2020.2649-2657 ◽

2020 ◽

Vol 13 (12) ◽

pp. 2649-2657

Author(s):

Berlin Pandapotan Pardede ◽

Muhammad Agil ◽

Yudi Yudi ◽

Iman Supriatna

Keyword(s):

Dna Fragmentation ◽

Artificial Insemination ◽

Semen Quality ◽

Fertility Rate ◽

Breeding Program ◽

Sperm Viability ◽

Production Site ◽

Frozen Semen ◽

Acrosome Integrity

Background and Aim: Various factors can reduce the quality of semen used for artificial insemination and have an impact on fertility decline, such as poor handling during frozen semen distribution. This study was aimed at assessing the quality of frozen-thawed semen after distribution in the field and its importance in maintaining fertility. Materials and Methods: The Brahman Cross (BX) breeding program of PT Lembu Jantan Perkasa, Indonesia, was used. This program was preferred due to its adherence to guidelines that limit the effects of extraneous factors that may affect semen quality. Frozen-thawed semen samples from eight bulls with the same production code were analyzed and compared between the production site (artificial insemination [AI] center) and the field (BX breeding program). Total and progressive motility (PM) of sperm were determined using computer-assisted semen analysis. Plasma membrane integrity (PMI) was assessed using hypoosmotic swelling test, sperm viability using Eosin-Nigrosin staining, acrosome integrity using trypan blue-Giemsa staining, morphological abnormalities using William staining, and DNA fragmentation using toluidine blue staining. The fertility rate was determined using the conception rate (%) derived from AI data based on 502 AI services and 478 cows in the BX breeding program. A t-test was used to compare the quality of frozen-thawed semen before and after distribution. The relationship between the qualities of frozen semen after distribution in the field with fertility was analyzed using Pearson correlation. Results: There was no significant difference (p>0.05) in the quality of frozen-thawed semen (sperm motility, PMI, viability, acrosome integrity, abnormalities, and DNA fragmentation) between the production site (AI center) and after distribution in the field (BX breeding program). The semen met the minimum standards for AI programs. Total motility (r=0.986), PM (r=0.961), sperm viability (r=0.971), PMI (r=0.986), and acrosome integrity (r=0.992) were all positively correlated (p<0.05) with fertility rate; while sperm abnormalities (r=-0.996) and sperm DNA fragmentation (r=0.975) were negatively correlated (p<0.05) with fertility rate. Conclusion: The study showed that to achieve the maximal and optimal fertility rate in bulls in an AI program, the overall quality of frozen-thawed semen in all aspects is critical. This can be achieved if the handling during distribution and storage, as well as the various factors that may affect the quality of semen in the field, can be controlled properly.

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The Influence of Air Contact on the Quality of Extended Boar Semen

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.81300 ◽

2018 ◽

Vol 44 (1) ◽

pp. 7

Author(s):

Caroline De Veronez Ribeiro ◽

Júlia Linck Moroni ◽

Mariana Boscato Menegat ◽

Ana Paula Gonçalves Mellagi ◽

Mari Lourdes Bernardi ◽

...

Keyword(s):

Sperm Motility ◽

Artificial Insemination ◽

Repeated Measures ◽

Semen Quality ◽

Random Effect ◽

Semen Parameters ◽

Swine Industry ◽

Subsequent Decrease ◽

Boar Semen

Background: The extensive use of artiﬁcial insemination in the swine industry emphasizes the need for distribution of good quality semen doses by the artiﬁcial insemination centers. A factor that could modify seminal quality is the contact of extended semen with environmental air. However, only few studies have investigated the effects of air presence on semen parameters. Thus, the aim of the present study was to evaluate the influence of different amounts of air on porcine semen quality during the storage period.Materials, Methods & Results: Four ejaculates from ﬁve sexually mature boars were used. Each ejaculate (n = 20) was isothermically diluted (30 x 106/mL) in a BTS-extender and assigned to three groups differing on the amount of air entrapped within the polystyrene tubes (100 mL). In group AIR-0 the tubes were completely ﬁlled with extended semen (0% air); in group AIR-25, 75% of the tube volume was ﬁlled with extended semen and 25% with air; in group AIR-50, 50% of the tube volume was ﬁlled with extended semen. The semen doses were stored at 17.3 ± 0.5ºC for 120 h. The motility parameters were assessed at 24, 72 and 120 h of storage using a CASA system. The evaluation of acrosomal integrity was performed by examining formalin-ﬁxed samples under phase-contrast microscopy (1000 x) at 72 and 120 h of storage. The pH was measured at 24, 48, 72, and 120 h of storage with a digital pH-meter. At 120 h, samples were incubated in waterbath at 38°C for the thermoresistance test and sperm motility was assessed after 30 and 120 min of incubation. For each time analysis, a different sealed tube was used in order to maintain a constant amount of air within the tubes during storage. Data were analyzed as repeated measures using the GLIMMIX procedure of SAS® Software. Boar and week of collection were included as random effect in the model. Groups were compared using the Tukey-Kramer test at a signiﬁcance level of 5%. The pH was signiﬁcantly influenced by the amount of air entrapped within the tubes (P < 0.01). Throughout the experiment, the pH of semen doses was higher as the amount of air increased within the tubes. The mean pH for groups AIR-50, AIR-25 and AIR-0 was 7.51 ± 0.06, 7.25 ± 0.06 and 7.07 ± 0.06, respectively. Overall motility was similar among the groups for up to 24 h of storage. At 72 h and 120 h of storage, total, progressive and rapid motility were higher (P < 0.05) in AIR-0 compared to AIR-50. The kinematic parameters DAP and VAP decreased as the volume of air within the tubes increased (P < 0.05). Group AIR-0 exhibited higher values (P < 0.05) for DSL, DCL, VSL, VCL, BCF, and WOB compared to AIR-25 and AIR-50. Furthermore, after the thermoresistance test performed at 120 h of storage, total motility, progressive motility and rapid motility were negatively affected (P < 0.05) by the increasing amounts of air within the tubes. There was no effect of air amount within the semen doses on acrosome integrity (P > 0.05).Discussion: The presence of air within tubes affects the quality of extended semen during storage, resulting in increase of pH and subsequent decrease in motility preservation. This pH-rise could be explained by the loss of CO2 from the diluted semen to the air compartment within the tube. At the same time, it is possible to infer that this event influences the intracellular pH of the spermatozoa, increasing sperm metabolism and, consequently, impairing sperm motility. Therefore, we conclude that avoiding air presence within storage containers is recommended in order to ensure the quality of boar semen doses.

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Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.43.1.26-34 ◽

2018 ◽

Vol 43 (1) ◽

pp. 26 ◽

Cited By ~ 1

Author(s):

H. C. Mahendra ◽

D. Samsudewa ◽

Y. S. Ondho

Keyword(s):

Artificial Insemination ◽

Recovery Rate ◽

Semen Quality ◽

Membrane Integrity ◽

Animal Husbandry ◽

Mean Value ◽

National Standard ◽

Frozen Semen ◽

Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value.

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The effect of semen collection frequency and food intake on semen production in breeding boars

Animal Science ◽

10.1017/s0003356100012885 ◽

1991 ◽

Vol 52 (2) ◽

pp. 355-360 ◽

Cited By ~ 6

Author(s):

B. Kemp ◽

G. C. M. Barker ◽

L. A. den Hartog ◽

M. W. A. Verstegen

Keyword(s):

Food Intake ◽

Artificial Insemination ◽

High Frequency ◽

Semen Quality ◽

Sperm Cells ◽

Feeding Level ◽

Semen Collection ◽

The Mean ◽

Effect Of Feeding

ABSTRACTAn experiment was conducted to study the effect of feeding level and semen collection frequency on semen quantity and quality of 62 Yorkshire boars at two artificial insemination centres during 10 successive periods of 2 weeks. The experiment had a 2 × 2 factorial design with two feeding levels (2·75 and 3·5 kg/day) and two semen collection frequencies (three and five times in 2 weeks).In the first 2-week period boars on the high frequency semen collection scheme produced 94 × 109 sperm cells more than boars on the low scheme. In periods 2 to 10 the mean difference was only 18 × 109 sperm cells. Boars on the high feeding level produced 32 × 109 sperm cells more per 2-week period than the boars on the low feeding level (P < 0·05). Boars at the high collection frequency and the high feeding level tended to produce a higher number of sperm cells during periods 2 to 10 than the boars on the high semen collection frequency and the low feeding level (P > 0·05). No effects of food intake or semen collection frequency on semen quality were found.

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