​Induction of Postpartum Oestrus in Lakhimi Cows through Hormonal and Nutritional Interventions

2021 ◽  
Author(s):  
C. Rahman ◽  
M. Bhuyan ◽  
L.J. Dutta ◽  
R. Deka ◽  
M. Baruti ◽  
...  
Keyword(s):  
Post Partum ◽  
Clomiphene Citrate ◽  
Conception Rate ◽  
Economic Losses ◽  
Genital Organ ◽  
Gnrh Analogue ◽  
Nutritional Interventions ◽  
Treatment Regimes ◽  
Therapeutic Regime ◽  
Mineral Mixture

Background: Postpartum anoestrus is the most devastating cause of the dairy farming which causes more economic losses to the farmers. The present study was conducted to record the incidence of postpartum anoestrus and to evolve a suitable therapeutic regime for addressing postpartum anoestrus in Lakhimi cows. Methods: A total of 1000 Lakhimi cows maintained in different villages of Assam were selected and the incidence rate was recorded on the basis of breeding records provided by the farmers, per-rectal examination and ultrasonography screening of genital organ. To study the effect of hormonal and nutritional interventions in post partum anoestrus cows, the cows were divided randomly into seven groups comprising twelve animals in each group. The different treatment regimes were Hydroxyprogesterone caproate + eCG, Clomiphene citrate alone, Clomiphene citrate + Mineral mixture, GnRH analogue alone, GnRH analogue + Mineral mixture, Mineral mixture alone and control. The response to different treatment regimes was based on oestrus response, mean interval from the end of treatment to onset of oestrus and conception rate. Result: The incidence of postpartum anoestrus was found to be 20.60%. Clomiphene citrate + Mineral mixture administration regime brought about the highest oestrus induction response (100.00%) and conception rate (66.67%).

Combating anestrous problem in crossbred cattle by nutritional interventions in field conditions of Odisha

2018 ◽  
Author(s):  
Debasish Satapathy ◽  
Sumant Kumar Mishra ◽  
Raj Kishor Swain ◽  
Kamdev Sethy ◽  
Tarun Kumar Varun ◽  
...  
Keyword(s):  
Average Daily Gain ◽  
Conception Rate ◽  
Crossbred Cattle ◽  
Nutritional Interventions ◽  
Mineral Supplementation ◽  
Ovarian Status ◽  
Mineral Mixture ◽  
T1 And T2 ◽  
Daily Gain ◽  
Bypass Fat

Through a survey in Kakatpur block, Odisha, twenty-one numbers of anestrous cattle were selected and equally divided in to three groups (T0, T1 and T2, n=7) for the experiment. T0 was kept as control (farmer’s practice), while T1 and T2 were provided with area specific mineral mixture (Ca, P, Mn, Cu, Zn) at 50g/day/animal and bypass fat at 100 g/day/animal, respectively for 60days. Average daily gain was significantly higher in T1 and T2 as compared to that of T0. Significant improvement in blood glucose, cholesterol, triglyceride and SGPT status was observed after various treatments. Due to mineral supplementation, all blood mineral status was improved. T1 and T2 groups exhibited better ovarian status and conception rate after treatment. Although, highest conception rate was observed in T2 group. Therefore, it can be concluded that supplementation of mineral mixture and bypass fat improved reproductive efficiency of anestrous animals along higher daily gain.

Effect of enhancement of uterine involution and earlier initiation of post-partum cyclicity on the reproductive performance of buffalo

2006 ◽  
Vol 18 (5) ◽  
pp. 545 ◽  
Author(s):  
A. A. Ramoun ◽  
S. A. Darweish ◽  
H. A. Abou El-Ghait ◽  
El-S. M. Fattouh
Keyword(s):  
Reproductive Performance ◽  
Intramuscular Injection ◽  
Post Partum ◽  
Chorionic Gonadotrophin ◽  
Calving Interval ◽  
Gnrh Analogue ◽  
Treatment Regimes ◽  
Uterine Involution ◽  
Gonadotrophin Releasing Hormone ◽  
Days Open

The aim of the present work was to study the effect of either gonadotrophin-releasing hormone (GnRH)–prostaglandin PGF2α–GnRH (G–P–G) or equine chorionic gonadotrophin (eCG)–PGF2α–GnRH (eCG–P–G) regimes with or without intramuscular injection of ergometrine maleate (Methergin) within 24 h post partum on the uterine involution, initiation of post partum cyclicity and subsequent reproductive performance of buffalo. A total of 60 parturient Egyptian buffalos (2–4 lactations) were used to conduct this study. The involved animals were allotted into two main groups (A and B), each with 30 animals, on the basis of whether buffalo cows received an i.m. injection of 3 mg of Methergin within the first 24 h post partum (A, Meth. T) or not (B, Meth. NT). Each main group was subdivided into three subgroups: A1, A2 and A3 in case of A and B1, B2 and B3 in case of B. The G–P–G regime was applied on both A1 (Meth. T) and B1 (Meth. NT) buffalos, where each individual received two i.m. injections of 0.020 mg GnRH analogue (buserelin) at Days 18 and 33 post partum with i.m. injection of 25 mg PGF2α (Dinoprost) in between at Day 25. The eCG–P–G regime was applied on both A2 (Meth. T) and B2 (Meth. NT) buffalos, where each individual received i.m. injection of 1000 IU eCG, 25 mg of PGF2α (Dinoprost) and 0.020 mg of GnRH analogue (buserelin) at Days 8, 18 and 25 post partum, respectively. The A3 subgroup (Meth. T, alone) was control for A1 and A2 subgroups and the B3 subgroup was the non-treated control for all subgroups. There was a significant (P < 0.01) decrease in the days required for complete uterine involution in subgroups A1, B1 and A3, indicating the enhancement of uterine involution. The post partum cyclicity was initiated earlier in all of the treatment regimes applied in the present study, as was indicated by significant (P < 0.01) decrease in the days to first ovulation in these subgroups compared with the non-treated control subgroup. The reproductive performance of treated buffalos improved as was shown by the significant (P < 0.01) decrease in days to first service, days open and calving interval. It is concluded that enhancement of uterine involution and/or earlier initiation of post-partum cyclicity improve the reproductive performance of buffalos.

Effect of Janova, Sepia and Ovsynch Protocol on Blood Biochemical Profile and Fertility in Postpartum Anoestrus Cows

2021 ◽  
Author(s):  
Jitendra Kumar ◽  
Sushant Srivastava ◽  
Rajesh Kumar ◽  
Govind Mohan ◽  
Vikas Chaudhiry
Keyword(s):  
Animal Husbandry ◽  
Diagnostic Value ◽  
Positive Control ◽  
Negative Control ◽  
Conception Rate ◽  
Biochemical Profile ◽  
Blood Biochemical ◽  
Technological Advances ◽  
Mineral Mixture ◽  
Oestrus Induction

Background: Anoestrus is still one of the most prevalent reproductive disorders in dairy cows despite technological advances in animal husbandry. Evaluation of blood biochemical profile is of diagnostic value to determine disease or health status of animal. The current study was aimed to evaluate the effect of different therapeutic regimen on blood biochemical profile, oestrus induction response and conception rate of anoestrus cows.Methods: Thirty two postpartum anoestrus cows randomly allocated to four groups as G0 (negative control), GI, GII, GIII, GIV and 8 normal cyclic as GV (positive control). Dewormer and mineral mixture administered to GI, GII, GIII, GIV while group GII, GIII and GIV additionally received janova, sepia and Ovsynch protocol respectively.Result: Different therapeutic protocols have variable effects on blood biochemical parameters. Overall oestrus induction response in G0, GI, GII, GIII, GIV and GV is 0.00, 50, 62.5, 75, 87.5 and 100.00 per cent respectively with corresponding conception rate of 0.00, 75, 80, 66.66, 57.13 and 75.00 per cent. On the basis of findings it can be concluded that aforesaid therapeutic regimens have definite bearings on fertility and can be used to manage postpartum anoestrus in cows.

Comparison of reproductive responses after PGF2α and GnRH-PGF2α oestrous synchronisation schemes in Holstein cows

2001 ◽  
Vol 26 (2) ◽  
pp. 467-470
Author(s):  
J.F. Cox ◽  
F. Saravia ◽  
O. Torrealba ◽  
A. Zavala ◽  
A. Lobos
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Detection Efficiency ◽  
Post Partum ◽  
Conception Rate ◽  
Milk Samples ◽  
Reproductive Response ◽  
Holstein Friesian ◽  
Breeding Schemes ◽  
Friesian Cows

AbstractControlled breeding schemes for oestrous detection constitutes a proactive technical response that balances the infrastructural requirement for a profitable dairy operation and the demands for optimal animal performance. The present study compared (a) the reproductive response of a treatment based on a short vs longer-acting PGF2α analogue (tiaprost vs luprostiol), and (b) the reproductive response after a treatment of GnRH-PGF2α vs PGF2α alone for synchronizing dairy cows. Holstein-Friesian cows averaging 9000 kg milk/lactation and fed according to their requirements were used in the study. Cows were cyclic, at least 60 days post partum and were clinically sound before being considered for the experiments. In Experiment 1, animals were synchronised using an i.m. injection of either 15 mg of luprostiol or 0.75 mg of tiaprost, based on ultrasonic diagnosis of a corpus luteum. Animals were inseminated at observed oestrus. In Experiment 2, cows were synchronised, at random, by either an injection of 10pg ofbuserelin (day 0) followed by 0.75 mg of tiaprost at day 7 (GnRH-PGF2α) orjust 0.75 mg of tiaprost (PGF2α). For both treatments only cows with an ultrasonically detected corpus luteum were treated. Animals were inseminated at oestrus. At the time of treatment and again 3 days later, milk samples were collected and assayed for progesterone by RIA. Cows with progesterone concentrations >1 ng/ml were considered to have corpus luteum. Luteolysis was considered to have occurred when concentrations of progesterone were > 1 ng/ml at day 0 and <0.8 ng/ml at day 3. In Experiment 1, both analogues gave similar results in terms of induced luteolysis [luprostiol: 36/39 (92.3%) vs tiaprost: 36/41 (87.8%)], oestrous detection efficiency [luprostiol: 26/36 (72.2%) vs tiaprost: 30/36 (83.3%], oestrous distribution [day 2, 3 and 4, respectively: luprostiol: 26.9%, 50.0%, 19.2% vs tiaprost: 36.7%, 50.0%, 13.3%], and conception rates [luprostiol: 12/25 (48.0%) vs tiaprost: 14/28 (50.0%); P>0.05]. In Experiment 2, oestrous detection efficiency, interval to oestrus and conception rate were similar between treatments [97/149 (65.1%), 71.1 h, 43/95 (45.3%) for PGF2α vs 130/188 (69.1%), 68.2h, 65/126 (51.6%) for GnRH-PGF2α, respectively]. However the oestrous distribution was more concentrated in GnRH-PGF2α treated animals (P<0.01).

scholarly journals Post Partum Pyometra In Iraqi Buffaloes : Clinical and Therapeutical Study

2005 ◽  
Vol 29 (1) ◽  
pp. 152-158
Author(s):  
A. Sh. Sultan
Keyword(s):  
Post Partum ◽  
Estradiol Benzoate ◽  
Conception Rate ◽  
Lugol’S Iodine ◽  
Days Open

The study was conducted on 118 buffaloes suffering from openedpyometra 20-30 days post partum in AL-thahab AL-abiadh village west ofBaghdad Province, their ages ranged from 3-6 years.They were divided randomly in to 4 groups . Group one included (27 buffalo)treated with 15 mg of PGF2α ( Prosolven )R IM, The 2nd group (32 buffalo)treated with 15 mg of PGF2α and 15 mg estradiol benzoate IM . The 3rd group(29 buffalo) treated with 15 mg of PGF2α and 50 . 100 ml of lugol’s iodine 0.5% intra-uterine.The 4th group (30 buffalo) treated with 15 mg of PGF2α and 4 gm ofOxyteracycline 20% (20 ml of Oxy. plus 50-100 ml disilled water) intra-uterine.Results showed that the 1st and 2nd response in 4 treated groups were66.2%, 84.3%, 79.3% and 86.6% respectively .The response was high in the 2ndand 4th group (P < 0.01). These responses represent also the conception rate forthese 4 treated groups. The means ± SE of days open for above groups were98.4± 6.4 ,84.2 ± 4.4 ,97.3 ± 3.8 and 82.7 ± 4.6 respectively ,the second andfourth group were significant (P < 0.01).The number of newly born calves was 94 calves (46 male,48 female) Thealive were 87(92.6%) and dead 7 (7.4%), so we conclude that the PGF2α has aneffective role in the treatment of pyometra in buffalo and it’s effect increasingwhen it combined with oxytetracycline 20% and estradiol benzoate together .

scholarly journals EFFECTS OF GnRH ANALOGUE AND VITAMIN AD3E ON INDUCTION OF CYCLICITY IN ANOESTRUS HEIFERS

2016 ◽  
Vol 13 (2) ◽  
pp. 33-40
Author(s):  
PK Sarker ◽  
MM Rahman ◽  
MMU Bhuiyan ◽  
M Shamsuddin
Keyword(s):  
Real Time ◽  
Body Condition Score ◽  
Conception Rate ◽  
Control Group ◽  
Gnrh Analogue ◽  
Treatment Groups ◽  
Drug Of Choice ◽  
Mineral Solution ◽  
Oestrus Induction ◽  
Real Time Ultrasonography

The purposes of this investigation were to determine the effects of different drugs such as GnRH analogue, vitamin AD3E and combination of vitamins and minerals on induction of cyclicity followed by conception in crossbred anoestrus heifers. The effects of body condition score (BCS) and age at anoestrus heifers during treatment on induction of cyclicity and conception were also determined. Sixty crossbred anoestrus heifers having > 2.5 BCS at >24 months age at Potiya Upazilla of Chittagong district were used. The cyclicity of the cows was confirmed by using real time ultrasonography on the basis of presence or absence of corpus luteum. The anoestrus heifers were allotted for 1 to 4 different treatment groups with GnRH analogue, vitamin AD3E and vitamin mineral solution including one no-treatment control group. AI was done in observed oestrus heifers and the conception was determined transrectally by using B-mode real time ultrasonography between 28-35 days post-Al. Heifers showed oestrus at higher (46.67%) proportion in treatment groups than that in no-treatment counterpart (6.67%) (P<0.05). The conception rate was 35.56% in treatment received heifers. The GnRH treated heifers showed the highest (60.00%) proportion of cyclicity and conception rate (46.67%) whereas vitamin mineral solution treated heifers showed the lowest cyclicity (33.33%) and conception rate (26.67%) (P<0.05). Heifers with BCS 3.5 had the highest (57.14%) rate of oestrus induction and (57.14%) rate of conception (P<0.05) whereas heifers with BCS 2.5 had the lowest (41.67%) rate of oestrus induction and (20.83%) rate of conception (P>0.05).  Heifer showed highest rate (50.00%) of cyclicity and (38.89%) rate of conception when their age ranges from 36 to 42 months where as heifer showed lowest (37.50%) rate cyclicity and (31.58%) rate conception when their age ranges from 24 to 29 months (P>0.05). In conclusion, treatment of crossbred anoestrus heifers with drugs has positive effect on induction of cyclicity followed by conception. GnRH may be the drug of choice among the drugs tested in this study. The BCS of heifers during treatment influences the treatment outcome with respect to induction of cyclicity followed by conception.

Influence of dietary cation-anionic difference on hematobio chemical profile, mineral metabolism, post-partum reproductive and productive performance of Hariana cows

2015 ◽  
Author(s):  
Bokan Abhay Mohanrao ◽  
Vinod Kumar ◽  
Debashis Roy ◽  
Muneendra Kumar ◽  
Mukesh Srivastava ◽  
...  
Keyword(s):  
Mineral Metabolism ◽  
Post Partum ◽  
Bos Indicus ◽  
Blood Calcium ◽  
Periparturient Period ◽  
S 100 ◽  
Mineral Mixture ◽  
Group Feeding ◽  
Periparturient Cows ◽  
And Performance

Eighteen multiparous periparturient Hariana cows (Bos indicus) (3-4 parity) in last month of their pregnancy were blocked into three groups (n=6) and fed for 30 days pre-partum to 30 days post-partum. The objective was to determine the effects of manipulating dietary cations and anions on hemato-biochemical profile, mineral metabolism and performance of cows. Nutrient requirements of cows were met by feeding concentrate and roughage. Varying levels of salt supplementation were used to formulate mineral mixture having DCAD of +11, +21 and +31 meq (Na+K - Cl+S) / 100 g of feed dry matter. Feeding DCAD of +11, +21 and +31 mEq/100 g of DM did not have any effect (P>0.05) on feed intake and body weight change during periparturient period. Blood calcium level was higher for the lowest DCAD (+11 meq/100 g of DM), suggesting that low DACD helps in mobilization of stored calcium in periparturient cows. pH of blood and urine increased curvilinearly with increasing DCAD and found maximum in +31 meq/100 g of DM DCAD fed group. Feeding of three different DCAD diets did not (P>0.05) have any effect on reproductive performance but milk yield was found significantly higher

scholarly journals Chronic Administration of the Metastin/Kisspeptin Analog KISS1-305 or the Investigational Agent TAK-448 Suppresses Hypothalamic Pituitary Gonadal Function and Depletes Plasma Testosterone in Adult Male Rats

Endocrinology ◽  
2012 ◽  
Vol 153 (11) ◽  
pp. 5297-5308 ◽  
Author(s):  
Hisanori Matsui ◽  
Akira Tanaka ◽  
Kotaro Yokoyama ◽  
Yoshihiro Takatsu ◽  
Kaori Ishikawa ◽  
...  
Keyword(s):  
Chronic Administration ◽  
Neural Response ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Genital Organ ◽  
Gnrh Analogue ◽  
Gonadal Function ◽  
Investigational Agent ◽  
Bilateral Orchiectomy ◽  
Gnrh Neurons

Abstract Metastin/kisspeptin, a hypothalamic peptide, plays a pivotal role in controlling GnRH neurons. Here we studied the effect of chronic sc administration of two kisspeptin analogs, KISS1-305 and TAK-448, on hypothalamic-pituitary-gonadal function in male rats in comparison with a GnRH analogue leuprolide or bilateral orchiectomy (ORX). The prototype polypeptide, KISS1-305 (1–4 nmol/h), caused substantial elevations of plasma LH and testosterone, followed by abrupt reductions of both hormone levels. Notably, testosterone levels were reduced to castrate levels within 3 d and remained depleted throughout the 4-wk dosing period, an effect that was faster and more pronounced than leuprolide (1 nmol/h) dosing. KISS1-305 also reduced genital organ weight more profoundly than leuprolide. In mechanistic studies, chronic KISS1-305 administration only transiently induced c-Fos expression in GnRH neurons, suggesting that GnRH-neural response was attenuated over time. Hypothalamic GnRH content was reduced to 10–20% of control at 3 wk without any changes in Gnrh mRNA expression. Dosing with the investigational peptide TAK-448 was also studied to extend our understanding of hypothalamic-pituitary functions. Similar to ORX, TAK-448 (0.1 nmol/h) depleted testosterone and decreased GnRH content by 4 wk. However, in contrast to ORX, TAK-448 decreased gonadotropin levels in pituitary and plasma samples, implying the suppression of GnRH pulses. These results suggest that chronic administration of kisspeptin analogs disrupts endogenous kisspeptin signals to suppress intrinsic GnRH pulses, perhaps by attenuating GnRH-neural response and inducing continuous GnRH leakage from the hypothalamus. The potential utility of kisspeptin analogs as novel agents to treat hormone-related diseases, including prostate cancer, is discussed.

Relationships among post-calving anoestrous interval, oestrous cycles, conception rates and calving date in Angus and Hereford × Friesian cows calving in six successive years

1993 ◽  
Vol 56 (2) ◽  
pp. 187-192 ◽  
Author(s):  
A. B. Pleasants ◽  
D. G. McCall
Keyword(s):  
Linear Regression ◽  
Mathematical Models ◽  
Post Partum ◽  
Oestrous Cycle ◽  
Conception Rate ◽  
Age Classes ◽  
Beef Cow ◽  
Cycle Lengths ◽  
The Mean ◽  
Friesian Cows

AbstractThe records of 1397 cows calving in the spring were collected in the years 1979 to 1984. They were from 638 Angus and 759 Hereford × Friesian cows, and were used to examine the dependencies of post-partum anoestrous interval (ppai), length of oestrous cycle after calving, and conception rate to each oestrus after calving.Two-year-old cows had longer ppai (P < 0·05) than older cows (76 v. 72 days), and Hereford × Friesian cows had shorter (P < 0·05) ppai than Angus cows (68 v. 72 days). Cows due to begin calving on 8 August (early calving) had longer (P < 0·01) ppai than cows due to begin calving on 19 September (71 v. 55 days).The linear regression between ppai and calving date (−0·60) did not differ between years, nor between early and late calving. Non-parametric regressions showed that the dependence between ppai and calving date was linear between plus or minus 20 days of the mean ppai in both early- and late-calving herds. Outside these limits variability of ppai increased and there was no relationship with calving date.The length of the first oestrous cycle after calving was 22 days in 2-year-old cows and 24 days in older cows. Second and third oestrous cycles after calving were 20 days for all breeds and age classes. There was a correlation of −0·41 between the ppai and the length of the first oestrous cycle after anoestrum. There was no significant deviation from normality of ppai or the succeeding post-partum oestrous cycle lengths.The conception rate to the first oestrus after calving was significantly lower (P < 0·01) than for later oestrous periods. The conception rate to first post-partum oestrus showed dependence on the ppai, logit regressions showing a 0·005 to 0·01 proportional improvement in conception at first oestrus per day increase in ppai.The significance of these results to the construction of mathematical models of reproduction in the beef cow is discussed.

Changes in albumin, globulin, glucose and cholesterol concentrations in the blood of dairy cows in late pregnancy and early lactation and relationships with subsequent fertility

1980 ◽  
Vol 94 (3) ◽  
pp. 517-527 ◽  
Author(s):  
G. J. Rowlands ◽  
R. Manston ◽  
A. J. Stark ◽  
A. M. Russell ◽  
K. A. Collis ◽  
...  
Keyword(s):  
Total Protein ◽  
Post Partum ◽  
Late Pregnancy ◽  
Conception Rate ◽  
Assay Method ◽  
Albumin Concentration ◽  
Liver Insufficiency ◽  
Average Decrease ◽  
Subsequent Fertility ◽  
Diffusion Assay

SummaryWeekly blood samples were collected from 42 Friesian cows between 5 weeks before and 10 weeks after calving, and analysed for serum cholesterol, albumin, total protein, glutamate-dehydrogenase, sorbitol-dehydrogenase and ornithine-carbamyl-transferase and blood glucose.Cholesterol concentrations increased 2½-fold during the first 8 weeks of lactation. There was also a transient decrease in glucose concentration at calving, but there was no relationship between conception rate and the concentrations of these two constituents.Albumin concentrations decreased at calving in some but not all cows and remained low for up to 2 weeks. Average concentrations of albumin determined by two analytical methods, a HABA dye-binding and a single radial diffusion assay method, were significantly lower (P < 0·05) between 0 and 2 weeks post-partum in eight cows which required four or more services than in 32 cows which conceived after fewer services. Similarly, the average decrease in concentration over calving was greater in those cows which conceived only after four or more services (P < 0·01).Globulin concentrations (total protein minus albumin) decreased during the 5 weeks before, and increased in the 3 weeks following calving. The net change over calving was significantly related to conception rate, and the albumin/globulin ratio was reduced (P < 0·001) in the eight cows requiring four or more services.Activities of all three enzymes doubled after calving, but there was no correlation between these increases and the decreases in albumin concentration, suggesting that, if liver insufficiency is a factor, it is probably due to malfunction rather than tissue damage.

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