scholarly journals Growth and Biochemical Variations in Macrotyloma Uniflorum Var. Madhu Under Chromium Stress

Author(s):  
Shilpee Dhali ◽  
, Madhusmit, Pradhan ◽  
Ranjan Kumar Sahoo ◽  
Chinmay Pradhan ◽  
Santanu Mohanty

The discharge of contaminated mining and industrial residues has become a major cause of environmental pollution. Seed is highly protective against external metal stresses. But they are highly sensitive during vegetative developmental process. In this study, germination was conducted in Macrotyloma uniflorum var. Madhu, in order to find out the effect of chromium toxicity on germination, growth and biochemical variations. The seeds were germinated in five different concentrations of chromium (25ìM, 50ìM, 100ìM, 150ìM and 200 ìM) solution. The hydroponically grown plants revealed the toxic phenotypic expression (root length, shoot length, seedling length, shoot vigor index, shoot fresh weight, shoot dry weight, root fresh weight and root dry weight) of plant after 3 growth stage (15 d, 30 d and 45 d). Similarly biochemical characteristics as well as antioxidant activities (Chlorophyll, carotenoid, carbohydrate, reducing sugar, catalase, proline content and peroxidase) were studied to see the toxic effect of chromium.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1150
Author(s):  
Arooj Fatima ◽  
Saddam Hussain ◽  
Sadam Hussain ◽  
Basharat Ali ◽  
Umair Ashraf ◽  
...  

Salinity and alkalinity stresses are common in arid and semiarid climates. Both these stresses not only retard crop growth but also cause a severe reduction in yields. The present experiment was performed to investigate the morphological, physiological, biochemical, and genetic responses of two maize hybrids (FH-1231 and DK-6714) to salinity and alkalinity stresses. The treatments were comprised of salt stress (NaCl:Na2SO4 at a 9:1 ratio), alkaline stress (NaHCO3:Na2CO3 at a 9:1 ratio), and an unstressed control. The results indicated that salinity and alkalinity significantly reduced shoot fresh weight by 50% and 70%, root fresh weight by 38% and 50%, root dry weight by 69% and 93%, seedling length by 18% and 30%, number of leaves by 27% and 39%, and maximum leaf width by 17% and 24%, respectively, across the two hybrids compared with control, indicating that alkalinity had a greater effect than salinity. Likewise, both the stresses, particularly alkalinity, significantly decreased K+ ion accumulation and chlorophyll content and increased the lipid peroxidation rate, sodium (Na+) concentration, the hydrogen peroxide (H2O2) level, and the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). Salinity and alkalinity stresses also induced the expression levels of antioxidant genes (SOD, CAT, POD, APX); however, salinity showed less effect than alkalinity stress. Similarly, hybrid DK-6714 performed comparatively better than FH-1231 with regard to seedling growth, antioxidant activities, and biochemical attributes under stress conditions. Thus, DK-6714 is recommended as a suitable hybrid for soils affected with salt-alkalization.


2020 ◽  
Vol 9 (1) ◽  
pp. 814-818

Effect of Carthamusoxyacantha powder aqueous extract on seed germination of Wheat and Maize were studied. Powder extract of 3g, 6g and 9g of concentration were soaked in distilled water for 12 Hrs, 24 Hrs and 36 Hrs of time. The seeds of Wheat and Maize were germinated in Petri dishes. Control was maintained by watering the seeds with distilled water. The highest values for germination percentage, shoot length, root length, fresh weight, dry weight, seedling length, vigor index and seedling weight vigor index were recorded in control of wheat the above parameter decreased with increasing concentration and timing. While in maize highest values for the above parameters were recorded in treatments as compared to control.


2011 ◽  
Vol 27 (3) ◽  
pp. 1295-1303 ◽  
Author(s):  
V. Mandic ◽  
Z. Tomic ◽  
V. Krnjaja ◽  
Z. Bijelic ◽  
M. Zujovic ◽  
...  

The aim of this investigation was to estimate the effect of four pH levels of germination media (4, 5, 6 and 7) on seed germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), percentage of hard seed (HS), normal (NS) and abnormal seedlings (AS), root length (RL), shoot length (ShL), seedling length (SeL), fresh weight (FW) and dry weight (DW) of seedling, and seedling vigor index (SVI) in two red clover genotypes (cv. K-17 and cv. Kolubara). The experiment was conducted in the laboratory conditions of the Institute for Animal Husbandry in Belgrade in January 2011. The pH levels of germination media had significant effect on the NS (P<0.01), AS (P<0.01), RL (P<0.05), ShL (P<0.01), SeL (P<0.01), FW (P<0.01) and SVI (P<0.05). The lowest GE, G, NS, ShL, SeL, FW and SVI were found at pH 4. Also, the highest DIS, HS and AS were found at pH 4. The genotype had significant effect on HS (P<0.01), RL (P<0.01), ShL (P<0.01), SeL (P<0.01), FW (P<0.01) and SVI (P<0.01). Genotype and pH levels of germination media did not affect on GE, G, DIS and DW significantly.


2014 ◽  
Vol 30 (2) ◽  
pp. 357-366 ◽  
Author(s):  
V. Mandic ◽  
Z. Bijelic ◽  
V. Krnjaja ◽  
Z. Tomic ◽  
A. Simic ◽  
...  

The aim of this research was to estimate the influence of different NaCl osmotic solutions (0, -0.3 MPa, -0.6 MPa, -0.9 MPa, -1.2 MPa, -1.5 MPa) on seed germination, and early seedling growth in two maize hybrid different maturity groups (ZP 560 - FAO 500 and ZP 666 - FAO 600). Germination was tested in sterile plastic vessels on filter paper moistened with different NaCl solutions, in the dark at 20 ? 1?C, in laboratory. Results of ANOVA indicated that hybrid ZP 666 had significantly higher root length (RL) (6.37 cm), shoot length (ShL) (2.06 cm), shoot fresh weight (ShFW) (43.86 mg), root dry weight (RDW) (7.56 mg), shoot dry weight (ShDW) (5.97 mg), seedling vigor index (SVI) (706.55) and dry matter stress tolerance index (DMSI) (55.85%) than hybrid ZP 560 (4.18 cm, 1.14 cm, 32.50 mg, 6.54 mg, 4.15 mg, 457.61 and 48.86%, respectively). Contrary, hybrid ZP 560 had significantly higher relative seedling water content (RSWC) (83.83%) and phytotoxicity of shoot (PhSh) (69.77%) than hybrid ZP 666 (81.16% and 62.51%, respectively). Generally, hybrid ZP 666 had better tolerance to salt stress than hybrid ZP 560. Germination energy (GE), germination (G), RL, ShL, root fresh weight (RFW), ShFW, RDW, ShDW, rate germination index (RGI), SVI, RSWC and DMSI were significantly decreased with the increase in osmotic stress induced by NaCl. Contrary, phytotoxicity of root (PhR) and PhSh significantly increased with the increase in osmotic stress.


2016 ◽  
Vol 74 (1) ◽  
pp. 37-44
Author(s):  
M. A. Alizadeh ◽  
H.A. Arab ◽  
R. Tabaie ◽  
M. Nasiri ◽  
A.A. Jafari

Abstract The seeds of 3 populations from Satureja bachtiarica were collected from three native vegetation areas (Simirom2, Sharkord and Yazd). The different treatments including: cold stratification, gibberlic acid (50 and 100 ppm) Potassium nitrate (0.2 and 0.4 percent). Physical scarification (sand paper), chemical scarification (Ethylic alcohol %70) and distilled water (control) were used for breaking dormancy of the seed samples. For greenhouse experiment, the seed samples were treated with treatments and then were sown in pots using randomize design with three replications. Emergence percentage, emergence speed, length of root and shoot, seedling length, root/shoot length ratio, vigor index, seedling fresh and dry weight, dry/fresh weight ratio were evaluated during 45 days of experiment. Comparing of population Semirom2 form savory of Bachtiary was higher than other population. It was concluded that effect of gibberlic acid and potassium nitrate was higher than physical scarification and chemical scarification comparing with control.


Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.


2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.


2012 ◽  
Vol 4 (2) ◽  
pp. 108-111
Author(s):  
Alireza PIRZAD ◽  
Mousa JAMALI ◽  
Mohammad Amin ZAREH ◽  
Fahime SHOKRANI

To evaluate probable allelopathic effect of water extract originated from different parts of russian knapweed (Acroptilon repens L.) on growth of purslane (Portulaca oleracea L.), a factorial experiment was conducted based on randomized complete block design with three replications at greenhouse condition in 2011. Treatments were water extract concentrations (0, 5, 10, 15 and 20 percent) obtained from different part of russian knapweed (aerial part, flower and root). Results showed the significant effect of extract type on seedling length, and extract concentration on shoot length, root length and seedling length. Interaction effect between extract type and concentration on the ratio of root/shoot length, seedling fresh weight, seedling dry weight and germination percentage was significant, too. Means comparison indicated that the longest root (3.55 cm), shoot (4.65 cm) and seedling (8.20 cm) were obtained from control treatment, reducing with higher concentration of extract. The longest shoot (6.95 cm) belonged to extract originated from russian knapweed flowers. The highest ratio of root/shoot length (0.73), seedling fresh weight (0.61g) and seedling dry weight (0.044 g) belonged to control treatment. In general, increasing of extract concentration caused in reduction of germination and seedling traits. Meanwhile, extracts of aerial part had more sever reducing effect than root and flower extracts.


Weed Science ◽  
1988 ◽  
Vol 36 (6) ◽  
pp. 775-778 ◽  
Author(s):  
Prasanta C. Bhowmik ◽  
Krishna N. Reddy

Field studies were conducted to determine the effects of various barnyardgrass populations on growth, yield, and nutrient concentration of transplanted “Jetstar’ tomato. Barnyardgrass densities at 16, 32, and 64 plants/m tomato row were tested in 1982 and 1983. Barnyardgrass shoot fresh weights/unit area increased as density increased. Fresh weight of barnyardgrass shoots ranged from 17 100 kg/ha at 16 plants/m of row to 35 500 kg/ha at 64 plants/m of row. At the vegetative stage, tomato shoot dry weight was unaffected by barnyardgrass. As crop growth progressed, tomato shoot dry weight decreased at all barnyardgrass densities. Season-long interference of barnyardgrass reduced marketable tomato fruit number and fruit weight at all densities compared to weed-free plots. Reductions in marketable fruit weight ranged from 26% to 16 plants/m row to 84% at 64 plants/m row. In 1982, concentrations of N, P, K, Ca, and Mg in tomato shoots were unaffected by season-long interference of barnyardgrass at all densities. However, in 1983, concentrations of N and K decreased and concentration of P increased in tomato leaves as the density of barnyardgrass increased. Concentrations of Ca and Mg in tomato leaves were unaltered by barnyardgrass density.


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