scholarly journals Drought Stress Responses of Prunus microcarpa C. A. Mey. subsp. tortusa Rootstocks under in vitro Conditions

2021 ◽  
Author(s):  
Nevzat Sevgin
Keyword(s):  
Chlorophyll A ◽  
Stress Responses ◽  
Economic Value ◽  
Control Treatment ◽  
Temperate Climate ◽  
Chlorophyll B ◽  
Peg Treatment ◽  
Semi Arid Region ◽  
Deciduous Plant

Background: Prunus microcarpa C.A. Mey. subsp. Tortusa (MK), a wild deciduous plant species, is native to Caucasus and West Asia and grows in a dry temperate climate zone. The plant is mainly used to obtain a dark greyish green dye from its leaves and it has an economic value due to ornamental characteristics. Therefore, this study aimed to determine the appropriate Polyethylene Glycol 8000 (PEG) doses for early detection of drought resistance using MK, SL-64, Garnem, Pixy and Gis-6 rootstocks.Methods: The microshoots of each rootstock were planted at the end of the fourth subculture in Magenta GA7 vessels containing NRM medium. The medium contained 1.0 mg L-1 BA + 0.01 mg L-1 IBA supplemented with 30 g L-1 sucrose, gelled with 5.5 g L-1 agar and 0, 2, 3 and 4% PEG doses (four treatments). The cultures were kept at 23±2 °C temperature under 16 h light (80 μmol m-2 s-1) and 8 h dark photoperiod.Result: The highest number of shoots was recorded in control treatment for MK rootstock (2.81 shoots plant-1) and in 4% PEG treatment for Gis-6 rootstock (2.81 shoots plant-1). Mean chlorophyll a (10.66 mg/f.w) and chlorophyll b (3.63 mg/f.w) contents in the control treatment were lower compared to the mean chlorophyll a (2.03 mg/f.w) and chlorophyll b (1.15 mg/f.w) contents of plants grown in PEG 4% dose. Prunus microcarpa genotypes of Turkey origin tested in this study could be used as potential rootstocks in arid and semi-arid region as safe as the other standard rootstocks tested.

scholarly journals Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants)

2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Economic Benefits ◽  
Chlorophyll B ◽  
Solvent Extraction Method ◽  
Chlorophyll A And B ◽  
The Right ◽  
Almost All ◽  
Chlorophyll A Content

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

Reduction of the Formyl Group of Zinc Pheophorbide b in vitro and in vivo: a Model for the Chlorophyll b to a Transformation

1996 ◽  
Vol 51 (3-4) ◽  
pp. 185-194 ◽  
Author(s):  
Verena Scheumann ◽  
Michael Helfrich ◽  
Siegrid Schoch ◽  
Wolfhart Rüdiger
Keyword(s):  
Nmr Spectroscopy ◽  
Chlorophyll A ◽  
Chemical Reduction ◽  
Chlorophyll B ◽  
Formyl Group ◽  
Pheophorbide A ◽  
Pheophytin A ◽  
H Nmr

Abstract The chemical reduction of the formyl group of pheophorbide b with sodium cyanoborohy­ dride in methanol leads to 71-methoxy-and 71-hydroxy-pheophorbide a. The same reaction with zinc pheophorbide b yields in addition zinc pheophorbide a. This was characterized by mass and 1H -NMR spectroscopy. Infiltration of zinc pheophorbides a and b and of zinc 71-hydroxy-pheophorbide a into etiolated oat leaves yielded phytylated products. The best yield in the esterification was obtained with 71-hydroxy-pheophorbide a. Analysis of the products revealed the formation of zinc pheophytin a from all infiltrated compounds. The significance for the transformation of chlorophyll b into chlorophyll a is discussed.

scholarly journals In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria

2019 ◽  
Vol 60 (12) ◽  
pp. 2672-2683 ◽  
Author(s):  
HyunSeok Lim ◽  
Ayumi Tanaka ◽  
Ryouichi Tanaka ◽  
Hisashi Ito
Keyword(s):  
Enzymatic Activity ◽  
Chlorophyll A ◽  
Developmental Stages ◽  
Enzymatic Activities ◽  
Light Acclimation ◽  
Chlorophyll B ◽  
Light Conditions ◽  
Chl A ◽  
Shallow Seas

Abstract In plants, chlorophyll (Chl) a and b are interconvertible by the action of three enzymes—chlorophyllide a oxygenase, Chl b reductase (CBR) and 7-hydroxymethyl chlorophyll a reductase (HCAR). These reactions are collectively referred to as the Chl cycle. In plants, this cyclic pathway ubiquitously exists and plays essential roles in acclimation to different light conditions at various developmental stages. By contrast, only a limited number of cyanobacteria species produce Chl b, and these include Prochlorococcus, Prochloron, Prochlorothrix and Acaryochloris. In this study, we investigated a possible existence of the Chl cycle in Chl b synthesizing cyanobacteria by testing in vitro enzymatic activities of CBR and HCAR homologs from Prochlorothrix hollandica and Acaryochloris RCC1774. All of these proteins show respective CBR and HCAR activity in vitro, indicating that both cyanobacteria possess the potential to complete the Chl cycle. It is also found that CBR and HCAR orthologs are distributed only in the Chl b-containing cyanobacteria that habitat shallow seas or freshwater, where light conditions change dynamically, whereas they are not found in Prochlorococcus species that usually habitat environments with fixed lighting. Taken together, our results implicate a possibility that the Chl cycle functions for light acclimation in Chl b-containing cyanobacteria.

scholarly journals Antioxidant properties of pepper (Capsicum annuum L.) depending on its cultivar and fruit colouration  

2021 ◽  
Author(s):  
Agnieszka Żurawik ◽  
Dorota Jadczak ◽  
Nikolay Panayotov ◽  
Piotr Żurawik
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Chlorophyll A ◽  
Antioxidant Properties ◽  
Temperate Climate ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Climate Conditions ◽  
Biological Value ◽  
Fruit Colouration

Due to its nutritional, health-promoting and taste-related values, new cultivars are introduced every year. The aim of the study was to assess the biological value of Polish and Bulgarian cultivars of pepper grown in moderate climate conditions and collected at different degrees of maturity. Ascorbic acid, carotenoids, polyphenols, chlorophyll a, chlorophyll b, total chlorophyll and antioxidant activity (DPPH) were determined in air-dried fruit. The investigation included five Polish cultivars: Roberta, Marta Polka, Etiuda, Trapez, and Cyklon and five Bulgarian cultivars: Bulgarski Ratung, Sivriya, Kurtovska Kapiya, Delikates, and Dzuliunska Shipka. Its design involved randomised sub-blocks, with three replications comprising nine plants each. The area of a single plot was 1.44 m<sup>2 </sup>(1.2 m × 1.2 m). The study confirmed the strong antioxidant properties of pepper grown in the field, without cover, and under temperate climate conditions. The vegetable is rich in vitamin C, polyphenols, carotenoids, chlorophyll pigments, and shows high antioxidant activity. However, the biological value of pepper is cultivar-dependent and is also determined by the fruit colouration degree. The coloured fruits are richer in vitamin C and carotenoids than the green ones, and when matured, they have greater antioxidant capacity. Green fruits contain more chlorophyll a, chlorophyll b and total chlorophyll than the coloured ones. The fruits of cv. Etiuda were the richest in vitamin C, of cv. Sivriya in polyphenols, of cv. Dzuliunska Shipka in carotenoids, and of cv. Trapez in total chlorophyll. Cv. Sivriya showed the strongest antioxidant properties.  

scholarly journals Effect of Drought-Stress Conditions in Chlorophyll Content of Dendrobium sp. Planlets

2019 ◽  
Vol 6 (1) ◽  
pp. 20-26
Author(s):  
Fesya Salma Putri ◽  
Endang Nurcahyani ◽  
Yulianty Yulianty ◽  
Bambang Irawan
Keyword(s):  
Drought Stress ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Economic Value ◽  
Total Content ◽  
Stress Conditions ◽  
Design Factor ◽  
Peg 6000 ◽  
Low Humidity

Dendrobium sp. was a popular ornamental plant and has high economic value. One of many difficulties in the growth of orchids in Indonesia is low humidity and lack of water availability. Regulatory Substance Growth in the form of an atonic solution can increase the growth of orchid plants. The purpose of this study was to determine the effect of atonic solutions on orchid chlorophyll content in drought stress conditions. This study used a 3x3 factorial design. Factor A is the atonic solution with 3 levels of concentration: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v and 25% b/v. The parameters tested were chlorophyll a, b and total Dendrobium orchid plantlets. The results showed that atonic solution and PEG 6000 significantly affected the chlorophyll a, b and total content of chlorophyll. The higher concentration of the atonic solution, the more content of chlorophyll a, b and total plantlets of decreased in drought stress conditions in vitro.

scholarly journals Effect Different Concentrations of Crude Oil on the Pigment Content and Protein Content of Hydrilla Verticillata Plant

2019 ◽  
pp. 2141-2148
Author(s):  
Sadiq Kadhum Lafta Alzurfi ◽  
Kasim Kadhim Alasedi ◽  
Noor Imad Abdulraheem
Keyword(s):  
Statistical Analysis ◽  
Crude Oil ◽  
Protein Content ◽  
Chlorophyll A ◽  
Hydrilla Verticillata ◽  
Gradual Decrease ◽  
Control Treatment ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Probability Level

The current study was conducted to determine the effect of crude oil on the pigments and protein content of Hydrilla verticillata plant from December 2018 to February 2019. Hydrilla verticillata was exposed to different concentrations of treatments (0.01,0.05,0.10,0.20) % of crude oil for 24 days. The pigments content was evaluated as total chlorophyll, a, b, carotene and protein content within plant tissues during the days (1, 8, 16 and 24).      The results of this study showed an increment in the total chlorophyll values of plant, which is exposed to different concentrations of crude oil compared to the control treatment during the first day of the experiment and decreased compared to control treatment during 8,16 and 24 days of the experiment. The recorded maximum value of total chlorophyll was (29.49) μg / g at 0.10% treatment during the first day and the statistical analysis showed significant differences below the probability level (P> 0.05) between the concentrations and the days of the experiment. Chlorophyll a, b and carotene of plant exhibit the overall trend of total chlorophyll. The values of chlorophyll b were higher than the values of chlorophyll and carotene.      The current study showed a gradual decrease in the total protein values of plants in crude oil during experiential period compared to control treatment, recording the highest values at the 0.01% during the first day (29.4 mg/g) and the lowest in the concentration of 0.05% during the day 24 was (3.5) mg / g, and the statistical analysis showed significant differences below the probability level (P> 0.05) between the days and concentrations of the experiment. The study concluded that a gradual decrease of total chlorophyll values during period experimental, recorded the chlorophyll b values higher compare to chlorophyll a and carotene. the gradual decrease of protein values with the increasing the concentration.

scholarly journals THE ALLELOPATHIC EFFECT OF MAIZE AND SORGHUM ON SOME PHYSIOLOGICAL TERISTICS OF WHEAT AND BROAD BEAN USING HYDROPONICS

2019 ◽  
Vol 25 (103) ◽  
pp. 90-118
Author(s):  
Hanaa H. Mohamed ◽  
Raghad M. Mirry
Keyword(s):  
Chlorophyll A ◽  
Chromatographic Analysis ◽  
Broad Bean ◽  
Control Treatment ◽  
Sand Culture ◽  
Soluble Carbohydrate ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Chlorophyll B ◽  
Allelopathic Potential

Two experiments were conducted to test the allelopathic potential of aqueous extracts concentrations of maize (Zea mays L.) and sorghum (Sorghum bicolor L.) on some physiological characteristics of wheat (Triticum aestivum L.) and broad bean (Vicia faba L.) plants in two cultures. The effective allelochemicals available in extracts of maize and sorghum have been isolated and identified. The experiments included two factors, the Main plots were the growing culture (Sand culture and Soil culture) and the Sub-plots were five concentrations of (0,25,50,75 and 100%) the aqueous extracts of the whole plants (root and shoot for maize (in first experiment) and sorghum (in the second one), Results indicated that: Most of the studied characteristics in wheat and broad bean decreased in sand culture when treated with the aqueous extracts of maize and sorghum. Increasing the aqueous extracts of maize to 100% decreased the plant content of  soluble carbohydrate, chlorophyll a, chlorophyll b, protein to 0.29 ,0.39, 0.39 and 0.28 % in wheat, and to 0.28 , 0.46 , 0.53 and 0.46 % in broad bean. While Increasing the aqueous extracts of sorghum to 100% decreased the plant content of  soluble carbohydrate, chlorophyll a, chlorophyll b, protein to 0.34 and 0.56 and 0.64 and 0.39 % in wheat, and to 0.41 and 0.47 and 0.44 and 0.20 % in broad bean, respectively  compared to control treatment (0%). Chromatographic analysis revealed the presence of 8 compounds in aqueous extracts of maize. The isolated compounds are phenolic in nature and known to have potential phytotoxicity as syringic, gallic, caffeic, vanillic, ferulic, coumaric, catechol and hydroxybenzoic. While Chromatographic analysis revealed the existence of 10 compounds in aqueous extracts of sorghum, 8 of them similar to what found in maize in addition to  quinon, benzaquinon

scholarly journals EFFECTS OF FOLIAR SPRAYING WITH SALICYLIC ACID ON SOME QUANTITY AND QUALITY CHARACTERISTICS OF PEAR FRUITS

2017 ◽  
Vol 7 (2) ◽  
pp. 1064-1070
Author(s):  
Ikbal Muhammed Gharib Tahir ◽  
Tahir A. Ahmed ◽  
Nameer N. Fadhil
Keyword(s):  
Salicylic Acid ◽  
Chlorophyll A ◽  
Block Design ◽  
Quality Characteristics ◽  
Fruit Weight ◽  
Control Treatment ◽  
Total Acidity ◽  
Chlorophyll B ◽  
Total Carotenoids ◽  
Highest Weight

A factorial experiment with randomized complete block design carried out in Erbil governorate, Iraq during 2015 and 2016 seasons on pear (Pyrus Communis L.) Spadona and Compote cvs. to investigate the effects of spraying trees with (0, 0.5, 1.0 and 1.5%) salicylic acid (SA) on some fruits quantity and quality characteristics. The results show that Compote fruits had the highest weight and firmness significantly compared to Spadona fruits in both seasons, whereas Spadona variety records the highest total acidity. Fruits of Compote variety records higher chlorophyll a , chlorophyll b and total carotenoids content significantly compared to Spadona variety in 2015, in the opposite of the results of 2016 in respect to chlorophyll a and chlorophyll b. Spraying trees with SA increased fruit weight and firmness compare to control treatment for both seasons, and total acidity in 2015 and total sugars in 2016, whereas the differences were not significant in 2016 and 2015 for the last two properties. In 2015 spraying 1.5% SA increased each of chlorophyll a and b content significantly compared to other concentrations, whereas significant decrease in total carotenoids content observed with increasing SA concentration, whereas the differences between SA treatments were not significant in 2016 in respect to chlorophyll a, b and total carotenoids.

Sign in / Sign up

Export Citation Format

Share Document