scholarly journals THE USE OF TRANSGENIC MOSQUITOES FOR PREVENTION OF SPREADOF ARBOVIRAL DISEASES

2019 ◽  
Vol 64 (3) ◽  
pp. 101-104
Author(s):  
G. G. Onishchenko ◽  
T. E. Sizikova ◽  
V. N. Lebedev ◽  
S. V. Borisevich
Keyword(s):  
Genetic Control ◽  
Sterile Insect Technique ◽  
Field Trials ◽  
Control Methods ◽  
Aedes Mosquitoes ◽  
Genetic Systems ◽  
Arboviral Diseases ◽  
Transgenic Technologies ◽  
Population Suppression ◽  
Transgenic Mosquitoes

The mosquitoes of Aedes genus are the most important vector such arboviral diseases as dengue, yellow, Chikungunya, West Nile and Zika fevers. Work is currently in progress to control the transmission of agents of these diseases by forming of transgenic mosquitoes in order to altering the capacity of wild mosquitoes to support of virus replication. There are two main strategies of genetic control of mosquitoes population. Sterile Insect Technique (SIT), that mainly uses population suppression methods for making self-sustaining genetic systems and Release of insects carrying of a Dominant Lethal (RIDL) that uses mainly gene transfer methods for making of self-limiting genetic systems. The RIDL is more expensive, but it has some significant preferences, according compares with SIT. The field trials of genetic control methods are conducted in several countries from 2009 to present time. Genetic control, transgenic technologies to induce sterility, genetic elimination and stable transformation of Aedes mosquitoes are viewed in this review.

scholarly journals Genetic Control of Mosquitoes: population suppression strategies

2012 ◽  
Vol 54 (5) ◽  
pp. 287-292 ◽  
Author(s):  
André Barretto Bruno Wilke ◽  
Mauro Toledo Marrelli
Keyword(s):  
Genetic Control ◽  
Mosquito Control ◽  
Public Health Problem ◽  
Mass Rearing ◽  
Control Measures ◽  
Reproductive Capacity ◽  
Lethal Gene ◽  
Control Methods ◽  
Species Specific ◽  
Population Suppression

Over the last two decades, morbidity and mortality from malaria and dengue fever among other pathogens are an increasing Public Health problem. The increase in the geographic distribution of vectors is accompanied by the emergence of viruses and diseases in new areas. There are insufficient specific therapeutic drugs available and there are no reliable vaccines for malaria or dengue, although some progress has been achieved, there is still a long way between its development and actual field use. Most mosquito control measures have failed to achieve their goals, mostly because of the mosquito's great reproductive capacity and genomic flexibility. Chemical control is increasingly restricted due to potential human toxicity, mortality in no target organisms, insecticide resistance, and other environmental impacts. Other strategies for mosquito control are desperately needed. The Sterile Insect Technique (SIT) is a species-specific and environmentally benign method for insect population suppression, it is based on mass rearing, radiation mediated sterilization, and release of a large number of male insects. Releasing of Insects carrying a dominant lethal gene (RIDL) offers a solution to many of the drawbacks of traditional SIT that have limited its application in mosquitoes while maintaining its environmentally friendly and species-specific utility. The self-limiting nature of sterile mosquitoes tends to make the issues related to field use of these somewhat less challenging than for self-spreading systems characteristic of population replacement strategies. They also are closer to field use, so might be appropriate to consider first. The prospect of genetic control methods against mosquito vectored human diseases is rapidly becoming a reality, many decisions will need to be made on a national, regional and international level regarding the biosafety, social, cultural and ethical aspects of the use and deployment of these vector control methods.

A Simple PCR Assay for Discrimination of Dengue Vectors from Nsukka LGA, Nigeria

2020 ◽  
Vol 10 (1) ◽  
pp. 67-77
Author(s):  
Amos Watentena ◽  
Ikem Chris Okoye ◽  
Ikechukwu Eugene Onah ◽  
Onwude Cosmas Ogbonnaya ◽  
Emmanuel Ogudu
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Mosquito Species ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Mosquito Vector ◽  
Aedes Mosquitoes ◽  
Allele Specific ◽  
Arboviral Diseases ◽  
Dengue Vectors

Mosquitoes of Aedes species are vectors of several arboviral diseases which continue to be a major public health problem in Nigeria. This study among other things, morphologically identified Aedes mosquitoes collected from Nsukka LGA and used an allele specific PCR amplification for discrimination of dengue vectors. Larval sampling, BG-sentinel traps and modified human landing catches were used for mosquito sampling in two selected autonomous communities of Nsukka LGA (Nsukka and Obimo). A total of 124 Aedes mosquitoes consisting of five (5) different species were collected from April to June, 2019 in a cross-sectional study that covered 126 households, under 76 distinct geographical coordinates. Larvae was mainly collected from plastic containers 73% (n=224), metallic containers 14% (n=43), earthen pots 9% (n=29) and used car tyres 3% (n=9), reared to adult stage 69.35% (n=86), and all mosquitoes were identified using standard morphological keys. Five (5) Aedes mosquito species were captured; Aedes aegypti 83(66.94%), Aedes albopictus 33(26.61%), Aedes simpsoni (4.48%), Aedes luteocephalus (≤1%) and Aedes vittatus (≤1%). Nsukka autonomous community had higher species diversity than Obimo. Allele specific amplification confirmed dengue vectors, Aedes aegypti and Aedes albopictus species on a 2% agarose gel. Since the most recent re-emergence of arboviral diseases is closely associated with Aedes species, findings of this study, therefore, give further evidence about the presence of potential arboviral vectors in Nigeria and describe the role of a simple PCR in discriminating some. Further entomological studies should integrate PCR assays in mosquito vector surveillance.

scholarly journals Genetic Analysis of Field Trials of Sex-linked Translocation Strains for Genetic Control of the Australian Sheep Blowfly Lucilia cuprina (Wiedemann)

1985 ◽  
Vol 38 (3) ◽  
pp. 275 ◽  
Author(s):  
GG Foster ◽  
WG Vogt ◽  
TL Woodburn
Keyword(s):  
Genetic Analysis ◽  
Genetic Control ◽  
Poor Performance ◽  
Field Trials ◽  
Lucilia Cuprina ◽  
Native Population ◽  
Australian Sheep ◽  
Males And Females ◽  
Progeny Tests ◽  
Release Method

The results of progeny tests of males and females captured during two field trials of sex-linked translocation strains for genetic control of L. cuprina are presented. Males released as mature larvae survived to adulthood and mated with field females. However, the levels of genetic death introduced into the population were insufficient to suppress the native population. This was due partly to seasonal ineffectiveness of the release method, and partly to poor performance of the released males. On average, the mating competitiveness of the released males was only one-third that of field males, whereas their field-reared, translocation-bearing sons were fully competitive with native males.

Dynamics of Wild and Sterile Mosquito Population Models with Delayed Releasing

2020 ◽  
Vol 30 (11) ◽  
pp. 2050218
Author(s):  
Li-Ming Cai
Keyword(s):  
Delay Differential Equations ◽  
Time Delays ◽  
Sterile Insect Technique ◽  
Sustained Oscillation ◽  
Hopf Bifurcations ◽  
Control Methods ◽  
Ode Models ◽  
Dynamical Features ◽  
The Stability ◽  
Delay Differential

To reduce the global burden of mosquito-borne diseases, e.g. dengue, malaria, the need to develop new control methods is to be highlighted. The sterile insect technique (SIT) and various genetic modification strategies, have a potential to contribute to a reversal of the current alarming disease trends. In our previous work, the ordinary differential equation (ODE) models with different releasing sterile mosquito strategies are investigated. However, in reality, implementing SIT and the releasing processes of sterile mosquitos are very complex. In particular, the delay phenomena always occur. To achieve suppression of wild mosquito populations, in this paper, we reassess the effect of the delayed releasing of sterile mosquitos on the suppression of interactive mosquito populations. We extend the previous ODE models to the delayed releasing models in two different ways of releasing sterile mosquitos, where both constant and exponentially distributed delays are considered, respectively. By applying the theory and methods of delay differential equations, the effect of time delays on the stability of equilibria in the system is rigorously analyzed. Some sustained oscillation phenomena via Hopf bifurcations in the system are observed. Numerical examples demonstrate rich dynamical features of the proposed models. Based on the obtained results, we also suggest some new releasing strategies for sterile mosquito populations.

scholarly journals Suppressing mosquito populations with precision guided sterile males

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ming Li ◽  
Ting Yang ◽  
Michelle Bui ◽  
Stephanie Gamez ◽  
Tyler Wise ◽  
...  
Keyword(s):  
Control System ◽  
Aedes Aegypti ◽  
Yellow Fever ◽  
Sterile Insect Technique ◽  
Life Stage ◽  
Molecular Genetic ◽  
Control Methods ◽  
Wild Populations ◽  
Principal Vector ◽  
Platform Technology

AbstractThe mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.

scholarly journals Investigation on the Occurrence of Aedes Species in Borderline of Iran and Azerbaijan for Control of Arboviral Diseases

2019 ◽  
Author(s):  
Eslam Moradi-Asl ◽  
Hassan Vatandoost ◽  
Davod Adham ◽  
Daryosh Emdadi ◽  
Hassan Moosa-Kazemi
Keyword(s):  
Cross Sectional Study ◽  
Suitable Habitat ◽  
Sectional Study ◽  
Cross Sectional ◽  
North West ◽  
The North ◽  
Aedes Mosquitoes ◽  
Arboviral Diseases ◽  
First Time ◽  
North Western

Background: To investigate the diversity of the genus Aedes present in the natural areas of Ardabil Province, north-west of Iran. Methods: This cross-sectional study was carried out from Apr to Oct 2016 in North-western of Iran. Thirty-three areas of 10 cities which are border areas were selected randomly. The larvae were collected 2 times in each month during the seasonal activities of mosquitoes and the larvae were identified morphologically according to the ap­propriate identification keys.  Results: Overall, 694 larvae were collected from four counties, from which only 7.2% were Aedes larvae. Three species of Aedes were identified which include Ae. caspius, Ae. vexans and Ae. flavescens. Aedes flavescens is reported from Ardabil Province for the first time. Conclusion: Aedes species were a high density in borderline of Iran and Azerbaijan. Therefore, the north parts of Ardabil Province are a suitable habitat for Aedes species mosquitoes. Care should be taken for vector control in the case of occurrence of any arboviruses transmitted by Aedes mosquitoes.

scholarly journals EFFECTS OF POULTRY MANURE AND WEED CONTROL METHODS ON GROWTH AND YIELD OF SESAME VARIETIES

2020 ◽  
Vol 4 (2) ◽  
pp. 8-15
Author(s):  
O. Danmaigoro ◽  
M. I. Zamfara ◽  
H. Yakubu ◽  
Musa M. Umar
Keyword(s):  
Plant Height ◽  
Weed Control ◽  
Seed Yield ◽  
Poultry Manure ◽  
Dry Weight ◽  
Field Trials ◽  
Growth And Yield ◽  
Control Methods ◽  
Number Of Leaves ◽  
Sudan Savanna

Field trials were conducted in 2017 and 2018 wet seasons at Federal University Dutse Teaching and Research Farm (Latitude 11 46, 39”N and Longitude 9 20, 30”E) in the Sudan Savanna of Nigeria. To evaluate performance of sesame varieties as affected by poultry manure and weed control methods. The treatments consisted of five weed control treatments (pre-emergence application of ButachlorEC50%,  hoe weeding at 3WAS +pre-emergence butachlor EC50%, pre-emergence butachlor EC50%+hoe weeding at 3 and 6WAS, hoe weeding @ 3 and 6WAS and weedy check), three level of poultry manure (5, 10, and 15t/ha) and three sesame varieties (Ben 01, Yandev 55 and Ben 04E. The treatments were laid out in split plot design and replicated three times. The results indicated that weed control methods had significant effect (0.05) on the sesame growth and seed yield comparable to hoe weeding control at 3 and 6WAS where plant height number of leaves,  capsule number per plant and seed yield of sesame were significant higher with the application of butachlor plus hoe weeding at 3 and 6WAS compared to others weed control methods.  Poultry manure application on sesame as 15t/ha gave taller plant height, more number of leaves, higher sesame dry weight, longer capsule length and seed yield of sesame than the other rates while Ben 01(455 and 1043 ) perform better than the others two varieties (Yandev 498, 756 and Ben4E 522 and 765 ) for 2017 and 2018 seasons.  In conclusion,       

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