BRAIN-HEART MEDIA FOR BLOOD CULTURES

2020 ◽  
Vol 65 (6) ◽  
pp. 375-381
Author(s):  
N. M. Kargaltseva ◽  
V. I. Kocherovets ◽  
A. Yu. Mironov ◽  
O. Yu. Borisova
Keyword(s):  
Blood Culture ◽  
Bloodstream Infection ◽  
Culture Media ◽  
Diagnostic Study ◽  
Blood Samples ◽  
Wide Range ◽  
Microbiological Control ◽  
The Media ◽  
Physical And Chemical

When diagnosing bloodstream infection (BI) the culture medium is the basis for growth of microorganisms and obtaining the blood culture. Pancreatic digest from fish meal is the basis of all culture media in Russia. In European countries brain-heart media (BHM) are used for detecting microorganisms in blood. In Russia BHM is not produced. The aim is to work out the formulation and the way of the BHM (broth and agar) preparation in order to improve the efficiency of obtaining blood culture. There were defined the physical and chemical indices and biological parameters of the BHM. The microbiological control of the BHM was carried out by diagnostic study of cardiological patients’ blood. On the basis of the developed technique of the brain-heart extraction (BHE) preparation there was created the liquid and agar BHM (LBHM, BHA). The LBHM was poured into bottles which then were filled with the inert gas. The bottles were closed with rubber stoppers and rolled in metal caps became a closed system. Microbiological qualities of LBHM were tested on 260 blood samples and thioglycollate medium (TGM) and LBHM. Aerobic microorganisms grew in LBHM 2,4 times more often than in TGM. The microaerophilic microbes grew in LBHM 3,2 times more often than in TGM. Anaerobic microbes did not show any growth in TGM, (p<0,001). Monomicrobes hemocultures were obtained in LBHM 13,4 times more often than in glucose broth and 2,3 times more often than in TGM, (p<0,001). Polymicrobes hemocultures were obtained in LBHM more often than in TGM (7,3% and 1,7%, respectively). The quality of brain-heart agar (BHA) was tested on 300 blood samples in 5% blood meat-pepton agar (MPA) and BHA in aerobic and anaerobic conditions for both the media. Aerobic microorganisms grew in BHA 2 times more often than in MPA. The microaerophilic microbes grew in BHA 3,6 times more often than in MPA. In anaerobic condition in BHA aerobic microorganisms grew 2 times more often than in MPA and the microaerophilic microbes grew 2,5 times more often than in MPA, (p<0,001). Anaerobic microbes did not grow in MPA. When diagnosing bloodstream infection the BHM (liquid and agar) are able to create the optimal conditions for the increase of the wide range pathogen growth.

Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos

2021 ◽  
Author(s):  
Gabriela de Oliveira Fernandes ◽  
Marcella Pecora Milazzotto ◽  
Andrei Antonioni Guedes Fidelis ◽  
Taynan Stonoga Kawamoto ◽  
Ligiane de Oliveira Leme ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biochemical Markers ◽  
Culture Medium ◽  
Culture Media ◽  
Ionization Mass ◽  
Metabolic Profiles ◽  
Bovine Embryos ◽  
The Media

Abstract The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy.

Six Sigma Methodology in Increasing Spirulina Production

Minerva ◽  
2020 ◽  
Vol 1 (1) ◽  
pp. 23-28
Author(s):  
Daniel Freire ◽  
Omar Flor ◽  
Gabriela Alvarez
Keyword(s):  
Six Sigma ◽  
Culture Media ◽  
Arthrospira Platensis ◽  
Lean Six Sigma ◽  
Growth Monitoring ◽  
Current State ◽  
Physical And Chemical Variables ◽  
Six Sigma Methodology ◽  
Physical And Chemical

This work presents results of improvement in the productivity of Arthrospira platensis (spirulina) in a company dedicated to its production. The six sigma methodology was applied in production processes that require the use of bioreactors. Starting from the analysis of the current state, aspects, physical and chemical variables that directly influence the productivity achieved were identified. Various culture media were tested and subsequently scaled for industrial production. In addition, the incorporation of carbon into the culture medium was controlled, optimizing the range of potential hydrogen pH. The identified parameters were measured and six sigma methodology strategies were assigned. An improvement in productivity corresponding to 66% was verified with the same quality of final product. Keywords: Six sigma, Bioreactors, Productivity, Arthrospira platensis. References [1]E. Ariawan and A. Makalew, “Smart Micro Farm: Sustainable Algae Spirulina Growth Monitoring System” in 10th International Conference on Information Technology and Electrical Engineering (ICITEE), Bali, 2018, pp.1-4. [2]L. Socconini and C. Reato, Lean six sigma: sistema de gestión para liderar empresas. Primera edición. Barcelona: Marge Books, 2019. [3]H. Gutiérrez, Calidad and productividad. Cuarta edición. México D.F.: McGraw-Hill Interamericana, 2014. [4]G. Usharani, P. Saranraj and D. Kanchana, “Spirulina Cultivation: A Review” in International Journal of Pharmaceutical & Biological Archives, vol. 3, no. 6, pp. 1327-1336, December 2012. [5]J. Udin, O. Gani, A. Mahato, I. Sakib and M. Rakiuzzaman, SPIRULINA (Spirulina platensis) PRODUCTION IN DIFFERENT PHOTOBIOREACTORS ON ROOFTOP, International Journal of Business, Social and Scientific Research, vol. 8, no. 1, pp. 15-19, January 2020. [6]M. Arredondo, Contabilidad y análisis de costos. Primera edición. México D.F.: Grupo Editorial Patria, 2015. [7]J. García, Contabilidad de costos. Cuarta edición. México D.F.: McGraw-Hill Interamericana, 2014. [8]L. Socconini, Certificación Lean Six Sigma Green Belt para la excelencia en los negocios. Primera edición. Barcelona: Marge Books, 2015. [9]A. Vian, Introducción a la Química Industrial. Segunda edición. Buenos Aires: Reverté, 2012. [10]S. Milton, Estadística para Biología y Ciencias de la Salud. Tercera edición. Madrid: McGraw-Hill Interamericana, 2014.

Obtaining Blood Cultures by Venipuncture versus from Central Lines Impact on Blood Culture Contamination Rates and Potential Effect on Central Line–Associated Bloodstream Infection Reporting

2013 ◽  
Vol 34 (10) ◽  
pp. 1042-1047 ◽  
Author(s):  
John M. Boyce ◽  
Jacqueline Nadeau ◽  
Diane Dumigan ◽  
Debra Miller ◽  
Cindy Dubowsky ◽  
...  
Keyword(s):  
Blood Culture ◽  
Bloodstream Infection ◽  
Hospital Costs ◽  
Cost Savings ◽  
Central Line ◽  
Potential Effect ◽  
Blood Cultures ◽  
Blood Samples ◽  
Central Lines ◽  
Contaminated Blood

Objective.Reduce the frequency of contaminated blood cultures that meet National Healthcare Safety Network definitions for a central line-associated bloodstream infection (CLABSI).Design.An observational study.Setting.A 500-bed university-affiliated hospital.Methods.A new blood culture policy discouraged drawing blood samples from central lines. Phlebotomists were reeducated regarding aseptic technique when obtaining blood samples by venipuncture. The intravenous therapy team was taught how to draw blood samples by venipuncture and served as a backup when phlebotomists were unable to obtain blood samples. A 2-nurse protocol and a special supply kit for obtaining blood samples from catheters were developed. Rates of blood culture contamination were monitored by the microbiology laboratory.Results.The proportion of blood samples obtained for culture from central lines decreased from 10.9% during January–June 2010 to 0.4% during July–December 2012 (P< .001). The proportion of blood cultures that were contaminated decreased from 84 (1.6%) of 5,274 during January–June 2010 to 21 (0.5%) of 4,245 during January–June 2012 (P< .001). Based on estimated excess hospital costs of $3,000 per contaminated blood culture, the reduction in blood culture contaminants yielded an estimated annualized savings of $378,000 in 2012 when compared to 2010. In mid-2010, 3 (30%) of 10 reported CLABSIs were suspected to represent blood culture contamination compared with none of 6 CLABSIs reported from mid-November 2010 through June 2012 (P= 0.25).Conclusions.Multiple interventions resulted in a reduction in blood culture contamination rates and substantial cost savings to the hospital, and they may have reduced the number of reportable CLABSIs.

scholarly journals Response of Zonal and Ivy Geraniums to Root Medium pH

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 766E-767
Author(s):  
Pauline H. Kaufmann* ◽  
P. Allen Hammer
Keyword(s):  
Dry Weight ◽  
Hydrated Lime ◽  
Research Data ◽  
Medium Ph ◽  
Root Medium ◽  
Wide Range ◽  
The Media ◽  
Garden Plant ◽  
Published Research

In 2002 the USDA reported potted geraniums accounted for $150 million in wholesale value, more than any other bedding or garden plant surveyed. Despite the importance of the geranium in floriculture production, little published research data is available pertaining to the media pH requirements of zonal and ivy geraniums. Current recommendations suggest zonal geraniums be grown at pH 5.7-6.6 and ivy geraniums at pH 5.0-6.2. The wide range in root medium pH recommendations for both zonal and ivy geraniums and the lack of research data prompted this research. Also, the basis for recommending a lower medium pH for ivy geraniums could not be found in published literature. The research objectives were to investigate the effect of medium pH on plant growth and to determine more precise recommendations for both species. The growth of 3 cultivars each of zonal and ivy geraniums growing in 8 medium pH treatments were evaluated. Limestone and hydrated lime were incorporated at increasing rates into a 1:1:1 peat, perlite and bark mix to achieve a medium pH ranging from pH 4.0-7.5. Plants were harvested at weeks 3, 6, and 11 and plant dry weight and media pH were determined. Leaf luminance, chroma and hue were evaluated at week 10. Plant dry weight was greatest at pH 6.55 or higher for both zonal and ivy geraniums at week 11. Leaves of plants grown at pH 6.55 or higher had significantly lower luminance and chroma and greater hue in all cultivars, corresponding to leaves that were darker, less vivid, and deeper green in color. This study shows a root medium pH greater than pH 6.5 results in greatest plant dry weight accumulation and quality of leaf color for both zonal and ivy geraniums. This study also shows ivy geraniums can be grown at the same media pH as zonal geraniums.

scholarly journals Method for evaluating broth culture media: application to Haemophilus

1978 ◽  
Vol 8 (5) ◽  
pp. 520-524
Author(s):  
A W Brinkley ◽  
T W Huber
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Research Laboratory ◽  
Culture Media ◽  
Nicotinamide Adenine ◽  
Broth Culture ◽  
Mueller Hinton ◽  
The Media ◽  
Growth Promoting ◽  
Mueller Hinton Broth

A method was devised to test the growth-promoting ability of a broth medium. The "dilute to extinction" method determines the inoculum required to develop heavy turbidity in a broth with overnight incubation. A statistical method using Poisson distribution was used to show that a single Haemophilus cell can develop heavy turbidity in an optimal broth. The dilute to extinction method was used to evaluate the shelf life of stored media, to titrate the growth factor requirements of Haemophilus, and to evaluate the use of purified hemin and nicotinamide adenine dinucleotide in a broth medium for the growth of Haemophilus. Of the media tested, the most suitable formulation was Mueller-Hinton broth supplemented with 10 microgram of hemin and 10 microgram of nicotinamide adenine dinucleotide per ml. The dilute to extinction method appears to be especially useful in the development of broth media for fastidious organisms. The method could also be used to assure the quality of other broth media which are required to support the growth of small inocula in the clinical or research laboratory.

scholarly journals Medium-dependent inhibition of Peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media

1976 ◽  
Vol 3 (4) ◽  
pp. 393-396
Author(s):  
T D Wilkins ◽  
S E West
Keyword(s):  
Escherichia Coli ◽  
Blood Culture ◽  
Inhibitory Concentration ◽  
Culture Media ◽  
Brain Heart Infusion ◽  
Medium Components ◽  
Proteose Peptone ◽  
Bactericidal Properties ◽  
Dependent Inhibition ◽  
The Media

Of 13 species of anaerobic cocci, Peptostreptococcus anaerobius was the only species tested that was sensitive to 0.1% sodium polyanetholsulfonate (SPS). However, the sensitivity of P. anaerobius to SPS varied according to the media in which the cultures were grown. In supplemented peptone (B-D) and brain heart infusion media, most strain of P. anaerobius were not inhibited by SPS. Gelatin and proteose peptone were the medium components which were protective. The minimal inhibitory concentration of SPS for P. anaerobius was approximately 60-fold higher in media. However, the concentration of SPS required to neutralize the bactericidal properties of human serum was only four fold higher in media containing geltain. In a commerical medium containing SPS (0.03%) and gelatin (1.2%), SPS-sensitive strains of P. anaerobius were not inhibited by SPS, and the bactericdal action of human blood on Escherichia coli C and Serratia marcescens SM 29 was eliminated.

scholarly journals THE USE OF FOOD ADDITIVE "MAHNETOFООD" IN THE TECHNOLOGY OF FORMED JELLY MARMELADE

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
I. Tsykhanovska ◽  
V. Yevlash ◽  
R. Trishch ◽  
T. Lazarieva ◽  
A. Alexandrov
Keyword(s):  
Aqueous Suspension ◽  
Food Additives ◽  
Food Additive ◽  
Gelling Agent ◽  
Technological Potential ◽  
Wide Range ◽  
Surface Active ◽  
The Stability ◽  
Physical And Chemical

Expansion and improvement of production of jelly and marmalade products requires the search for simplified resource- and energy-saving technology, increasing the stability of a colloidals dispersed system and improving the quality of finished products. Promising technological applications have food additives in the nanometer range, due to unique physical and chemical parameters and a wide range of functional and technological properties. The paper proposes a solution to the problem of stabilizing the colloidal structure of jelly marmalade products and the formation of their quality by using a food additive "Magnetofood" (based on oxides of divalent and trivalent iron: FeO·Fe2O3). "Magnetofood" – ultrafine powder with a particle size of ~80 nm, which has a high functional and technological potential: large specific surface area, surface-active, complexing, sorption, stabilizing properties. This allows us to recommend "Magnetofood" as a food additive of complex action to improve the quality and extend the shelf life of jelly marmalade products, in particular molded jelly marmalade. It is established that the addition of food additive "Magnetofood" in the form of an aqueous suspension at the stage of "swelling-dissolution" of the gelling agent improves the shape, surface and consistency; increases the moisture content by 1,15–1,16 times in agar samples and 1,13–1,14 times in pectin samples; reduces the total acidity by 1,05–1,08 times in agar samples and 1,06–1,09 times in pectin samples; inhibits microbial contamination of the surface of marmalade samples. In addition, the rational content of food additive "Magnetofood" – 0,15% by weight of the prescription mixture and the optimal conditions of temperature and humidity during storage of molded jelly marmalade for 90 days: relative humidity φ=(75±2)%, temperature (18±2)oC.

scholarly journals TiO2: A Semiconductor Photocatalyst

2021 ◽  
Author(s):  
Azrina Abd Aziz ◽  
Fatema Khatun ◽  
Minhaj Uddin Monir ◽  
Sim Lan Ching ◽  
Leong Kah Hon
Keyword(s):  
Tio2 Nanoparticles ◽  
Anatase Tio2 ◽  
Band Gaps ◽  
Theoretical Concepts ◽  
Wide Range ◽  
Phase Size ◽  
Physical And Chemical ◽  
Shape Of Particles ◽  
Semiconductor Photocatalyst

Titanium dioxide (TiO2) is considered as an inert and safe material and has been used in many applications for decades. TiO2 have been widely studied, due to its interesting general properties in a wide range of fields including catalysis, antibacterial agents, in civil as nano-paint (self-cleaning) and especially photocatalysis, and that affect the quality of life. Thus, the development of nanotechnologies TiO2 nanoparticles, with numerous novel and useful properties, are increasingly manufactured and used. TiO2 doped with noble metal are good candidates in the performance these applications. The fascinating physical and chemical features of TiO2 depend on the crystal phase, size and shape of particles. For example, varying phases of crystalline TiO2 have different band gaps that rutile TiO2 of 3.0 eV and anatase TiO2 of 3.2 eV, determine the photocatalytic performance of TiO2. This chapter explains basic information on TiO2 and theoretical concepts of nanostructure of TiO2 nanoparticles as a semiconductor photocatalyst.

Single or Group Culture of Mammalian Embryos: The Verdict of the Literature

2011 ◽  
Vol 2 (2) ◽  
pp. 77-87 ◽  
Author(s):  
Michael L. Reed ◽  
Bryan J. Woodward ◽  
Jason E. Swain
Keyword(s):  
Embryo Culture ◽  
Negative Impact ◽  
Culture Media ◽  
Group Culture ◽  
Mammalian Embryos ◽  
Potential Benefits ◽  
The Media ◽  
To Come ◽  
Embryotrophic Factors

During infertility treatment with IVF, embryos are cultured either in groups or individually. Each approach has potential benefits and detriments, and the purpose of this review is to try to come to a consensus based on the literature as to which approach yields superior results. Group culture of embryos may produce better quality embryos via secretion of embryotrophic factors, while opponents of the approach argue that embryos cultured together may either deplete the media of substrates or negatively affect nearby embryos via the transmission of other secreted factors. In these cases, quantity of embryos, volume of media and proximity and quality of companion embryos are also important factors to consider. While it has long been accepted that group culture is beneficial for embryos from various animal species, emerging data also suggest a similar benefit in the human. Conversely, embryos cultured individually avoid potential substrate depletion, negative impact from factors secreted from companion embryos, while more practically permitting the ability to monitor and track the embryo for identification via morphology or molecular analysis to select and transfer potentially superior embryos. Importantly, advancements in embryo culture platforms now permit tracking of individual embryos, while also offering ability to reap the benefits of group culture. These approaches utilize confined microenvironments immediately surrounding the embryos that may be conducive for periodic sampling/analysis, while also allowing access to a larger media reservoir to avoid substrate depletion. Thus, though questions remain as to optimal embryo density and volume of culture media, group embryo culture in the correct culture platform is likely to be superior to individual embryo culture.

scholarly journals Kebijakan Redaksional MQTV Bandung dalam Penyiaran Berita

2017 ◽  
Vol 1 (1) ◽  
pp. 99-118
Author(s):  
Adib Darissalam
Keyword(s):  
Culture Media ◽  
Rapid Development ◽  
Religious Life ◽  
Media Culture ◽  
Major Question ◽  
Media System ◽  
Wide Range ◽  
The Media ◽  
The Relationship ◽  
Positive Effect

The religious life is experiencing rapid development in conjunction with the revolutionary changes in the media. At the stage of modernization, entered the twentieth century, the development of the media is an industry with a wide range of cultures that make it up. But when the religious interests collide with culture media system, there was friction that easily trigger sensitivity. Harmonization of religion and culture media, which never happened in the centuries to write the media triumph, now it is often disturbed and provoked by the dynamics posed by culture media. In the context of the relationship between religion and culture of this media, one major question that deserves to be answered in this paper is; how to bridge the interests of religion (as something divine, sacred and ceremonial) with the media culture as an industrial capitalist. What implications arise and which will result from this relationship. Will a positive effect on the religious or otherwise.

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