scholarly journals THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 38
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p><strong>Keywords</strong>: MSG, curcuma, endometrium</p>

THEEFFECTS OF CURCUMA RHIZOME EXTRACT (Curcuma xanthorrhiza Roxb.)TREATMENT USING VARIOUS DOSES TOWARDS ENDOMETRIAL THICKNESSIN MICE (Mus musculus) TREATED WITH MONOSODIUM GLUTAMATE (MSG)

2017 ◽  
Vol 2 (1) ◽  
pp. 39
Author(s):  
Fidia Rizkiah Inayatilah
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Endometrial Thickness ◽  
Monosodium Glutamate ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

<p><em>Monosodium Glutamate </em>(MSG) is a food seasoning that is widely used. Consumption of MSG in excessive doses can cause an excess of free radicals in the body that trigger oxidative stress and finally affect the reproductive system hormones. These free radicals can be suppressed by administration of antioxidants. Rhizomes of Curcuma xanthorrhiza extracts containantioxidants and they are widely grown. Theyare used as traditional medicine in Indonesia. This experiment was conducted to investigatewhether rhizomes of Curcuma xanthorrhiza extracts areable to inhibit decreasing endometrial thickness in female mice after receiving orally MSG-treatment.This research adoptedtrue experimental posttest only control group design<em>. </em>It includes 25 mice that were divided into 5 groups: control K1 (CMC) and K2 (MSG), treatment group (MSG and rhizomes of Curcuma xanthorrhiza extracts with dose of 0,4 mg/gBW, 0,8 mg/gBW, 1,2 mg/gBW).In addition, the mice’s endometrium were removed to be subsequently stained using Hematoxillin-Eosin. Thickening of uterus was observed and measured with 40x magnitude using Microphoto Microscope Olympus. Data was analized using One Way ANOVA. The research finally comes with a result showing that there were not any significant differences discovered from the endometrium thickening from the five tested groups (p&gt;0,05). Therefore, it is implied that rhizomes of Curcuma xanthorrhiza extracts has no effect on endometrial thickness of uterus by oral MSG-treatment.</p><p> </p><p>Key words: MSG, curcuma, endometrium</p>

scholarly journals Pengaruh pemberian vitamin E terhadap kadar neutrofil setelah latihan fisik

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kristo Warong ◽  
Damajanty H.C. Pangemanan ◽  
Joice N.A. Engka
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Physical Exercise ◽  
Tissue Injury ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Test Control

Absract: Physical exercise can improve and maintain physical fitness. However, physical exercise can lead to oxidative stress that can reduce the activity of antioxidants. Vitamin E is a fat-soluble antioxidant that could free radicals in the body. Neutrophils play an active role in the process of phagocytosis of bacteria and other microorganisms the damaged tissue caused by tissue injury. This study was aimed to obtain the effect of vitamin E on neutrophil count after physical exercise. This was a field experimental study with a pre post test control group design. Data were analyzed with the Mann Whitney U (α = 0.05). The physical exercise was playing futsal for 60 minutes. Respondents were 30 male respondents divided into 2 groups: treatment and control groups. The levels of neutrophils were examined after physical exercise and after the administration of vitamin E 400 IU for seven days. Data analysis of the effect of vitamin E on neutrophil level after physical exercise showed a p value of 0.031. Conclusion: Vitamin E influenced the levels of neutrophils after physical exercise.Keywords: physical exercise, oxidative stress, vitamin E, neutrophil Abstrak: Latihan fisik merupakan aktivitas yang dilakukan seseorang untuk meningkatkan atau memelihara kebugaran tubuh. Latihan fisik dapat menimbulkan stres oksidatif sehingga dapat menurunkan aktivitas antioksidan. Vitamin E merupakan antioksidan yang larut dalam lemak dan berfungsi untuk mengurangi radikal bebas yang terdapat dalam tubuh. Neutrofil berperan aktif dalam proses fagositosis bakteri, mikroorganisme, dan membersihkan sisa jaringan rusak yang disebabkan oleh cedera jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Jenis penelitian ialah eksperimental lapangan dengan pre post test control group design. Untuk menguji signifikansi penelitian digunakan uji Mann Whitney U (α = 0,05). Latihan fisik berupa olahraga futsal selama 60 menit dilakukan oleh 30 responden laki-laki yang dibagi dalam 2 kelompok yaitu perlakuan dan kontrol. Kadar neutrofil diukur setelah latihan fisik dan setelah pemberian vitamin E 400 IU selama 7 hari. Hasil statistik menunjukkan terdapat pengaruh bermakna dari vitamin E pada kelompok perlakuan (p=0,031). Simpulan: Terdapat pengaruh vitamin E terhadap kadar neutrofil setelah latihan fisik. Kata kunci: latihan fisik, stres oksidatif, vitamin E, neutrofil

scholarly journals THE EFFECT OF ADMINISTERING RHIZOME EXTRACT OF CURCUMA (Curcuma Xanthorrhiza Roxb.) AT VARIOUS DOSES ON THE THICKNESS OF VAGINAL EPITHELIUM IN MICE (Mus Musculus) EXPOSED TO MSG

2017 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Ria Ramadhani Dwi Atmaja
Keyword(s):  
Monosodium Glutamate ◽  
Vaginal Epithelium ◽  
The Body ◽  
Control Group ◽  
Significant Difference ◽  
The Us ◽  
Excessive Consumption ◽  
Curcuma Xanthorrhiza ◽  
Flavour Enhancer ◽  
One Way Anova

<p><em>The us</em><em>age</em><em> of Monosodium Glutamate (MSG) as flavour enhancer in food is increasing and has reached alarming condition. Excessive consumption of MSG results in the formation of a number of free radicals in the body and affects the function of hypothalamus. A disturbed hypothalamus results in the decline of estrogen levels, and in turn, this estrogen deficiency causes a decrease in vaginal epithelial proliferation in which epithelial cells become thinner. Giving antioxidants might help to address these problems. One of the plants that is rich in antioxidant and grow in mass in Indonesia is curcuma. This study is aimed at analyzing the effect of giving curcuma extract (Curcuma Xanthorrhiza Roxb.) with varying doses on the thickness of vaginal epithelium in mice that are exposed to MSG. The research design employed in the study is true experimental posttest only control group by using 5 groups, namely: K1 (CMC-Na), K2 (MSG), P1 (MSG + curcuma rhizome extract dose of 0,4mg/gr BW), P2 (MSG + curcuma rhizome extract dose of 0,8mg/gr BW), P3 (MSG + curcuma rhizome extract dose of 1,2mg/gr BW). Vagina of each mouse was taken and studied to measure its epithelial thickness by using a 40x magnification on Olympus micrograph microscope. Data were then analyzed using One Way ANOVA. The results show that there is no significant difference in the thickness of vaginal epithelium in each treatment (ρ-value 0.341). Therefore, it can be concluded that the extract of curcuma rhizome does not have a significant influence on the thickness of vaginal epithelium in mice that are exposed to MSG.</em><em></em></p><p> </p><table border="0" cellspacing="0" cellpadding="0"><tbody><tr><td valign="top" width="432"><p><strong>Keywords :</strong><em> MSG, Curcuma Xanthorrhiza, epithelium, vagina.</em></p></td></tr></tbody></table>

scholarly journals THE EFFECT OF ADMINISTERING RHIZOME EXTRACT OF CURCUMA (Curcuma Xanthorrhiza Roxb.) AT VARIOUS DOSES ON THE THICKNESS OF VAGINAL EPITHELIUM IN MICE (Mus Musculus) EXPOSED TO MSG

2017 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Ria Ramadhani Dwi Atmaja
Keyword(s):  
Monosodium Glutamate ◽  
Vaginal Epithelium ◽  
The Body ◽  
Control Group ◽  
Significant Difference ◽  
The Us ◽  
Excessive Consumption ◽  
Curcuma Xanthorrhiza ◽  
Flavour Enhancer ◽  
One Way Anova

<p><em>The us</em><em>age</em><em> of Monosodium Glutamate (MSG) as flavour enhancer in food is increasing and has reached alarming condition. Excessive consumption of MSG results in the formation of a number of free radicals in the body and affects the function of hypothalamus. A disturbed hypothalamus results in the decline of estrogen levels, and in turn, this estrogen deficiency causes a decrease in vaginal epithelial proliferation in which epithelial cells become thinner. Giving antioxidants might help to address these problems. One of the plants that is rich in antioxidant and grow in mass in Indonesia is curcuma. This study is aimed at analyzing the effect of giving curcuma extract (Curcuma Xanthorrhiza Roxb.) with varying doses on the thickness of vaginal epithelium in mice that are exposed to MSG. The research design employed in the study is true experimental posttest only control group by using 5 groups, namely: K1 (CMC-Na), K2 (MSG), P1 (MSG + curcuma rhizome extract dose of 0,4mg/gr BW), P2 (MSG + curcuma rhizome extract dose of 0,8mg/gr BW), P3 (MSG + curcuma rhizome extract dose of 1,2mg/gr BW). Vagina of each mouse was taken and studied to measure its epithelial thickness by using a 40x magnification on Olympus micrograph microscope. Data were then analyzed using One Way ANOVA. The results show that there is no significant difference in the thickness of vaginal epithelium in each treatment (ρ-value 0.341). Therefore, it can be concluded that the extract of curcuma rhizome does not have a significant influence on the thickness of vaginal epithelium in mice that are exposed to MSG.</em><em></em></p><p> </p><p><strong>Keywords :</strong><em> MSG, Curcuma Xanthorrhiza, epithelium, vagina.</em><em></em></p>

scholarly journals Gambaran histologik ginjal tikus Wistar yang diberikan jus tomat setelah diinduksi dengan monosodium glutamat

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Desy Togatorop ◽  
Taufiq F. Pasiak ◽  
Djon Wongkar ◽  
Martha M. Kaseke
Keyword(s):  
Free Radicals ◽  
Vitamin A ◽  
Vitamin C ◽  
Wistar Rats ◽  
The Body ◽  
Tomato Juice ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstract: Monosodium glutamate (MSG) is widely used in daily life as a flavor enhancer in food. MSG is the combination of sodium salt component and glutamic acid-L (a non essential amino acid), which is highly soluble in water. Glutamate in MSG is not bound to protein molecules, but in the free form, therefore, it will form free radicals in the body. Free radicals in the body can be neutralized by antioxidants. Tomatoes are fruits that contain several antioxidants (lycopene, vitamin C and vitamin A). Lycopene is an antioxidant that is the highest in tomatoes. Lycopene reduces free radicals in the body by releasing up one of its electrons to bind with free radicals so the free radicals do not bind to other cells in the body and reduces the damage in the body. This study was aimed to determine the histological changes in the kidneys of Wistar rats induced by MSG, and of those that were added tomato juice. This was an experimental study with a post test only control group design. The study used 15 Wistar rats divided into three groups: group I without treatment (pellets AD II and drinking water); group II, MSG for 14 days; and group III, tomato juice and MSG for 14 days. The results showed that in both treatment groups there were visible swelling of tubular epithelial cells and narrowing of the tubular lumen, albeit, the glomeruli still looked normal.Keywords: tomato juice, MSG, kidney damage Abstrak: Monosodium glutamat (MSG) banyak digunakan sebagai penyedap rasa dalam makanan. MSG adalah gabungan antara komponen garam sodium dan asam glutamat–L (suatu asam amino non esensial) yang bersifat sangat larut dalam air. Glutamat dalam MSG tidak terikat pada molekul protein melainkan dalam bentuk bebas sehingga dapat membentuk radikal bebas didalam tubuh. Radikal bebas didalam tubuh dapat dinetralisir oleh antioksidan. Tomat mengandung berbagai antioksidan (likopen, vitamin C dan vitamin A) terutama likopen. Likopen mengurangi radikal bebas didalam tubuh dengan cara melepaskan satu elektronnya untuk berikatan dengan radikal bebas yang dapat mengurangi kerusakan yang terjadi dalam tubuh. Penelitian ini bertujuan untuk mengetahui gambaran histologik ginjal tikus wistar kelompok perlakuan I diinduksi MSG dan kelompok perlakuan II yang diberikan MSG dan jus tomat. Jenis penelitian ialah eksperimental dengan post test only control group design. Penelitian menggunakan 15 ekor tikus wistar dibagi tiga kelompok: kelompok I tanpa perlakuan (pellet AD II dan air minum); kelompok II diberi MSG selama 14 hari; dan kelompok III diberi jus tomat dan MSG selama 14 hari. Gambaran histologik ginjal pada kelompok perlakuan I (MSG) dan kelompok perlakuan II (MSG dan jus tomat) memperlihatkan glomerulus normal, pembengkakan sel epitel tubuli, dan penyempitan lumen tubuli. Tidak tampak perbedaan nyata antara kedua kelompok. Kata kunci: jus tomat, MSG, kerusakan ginjal

scholarly journals EFEK ANTIMIKROBA EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza) TERHADAP PERTUMBUHAN Staphylococcus aureus

2017 ◽  
Vol 10 (2) ◽  
pp. 138
Author(s):  
Ardiana Retno Mashita
Keyword(s):  
Staphylococcus Aureus ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Curcuma Xanthorrhiza ◽  
One Way Anova

Efek Antimikroba Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza) Terhadap Pertumbuhan Staphylococcus aureus. Staphylococcus aureus merupakan patogen utama pada manusia yang bersifat invasif. Pengobatan Staphylococcus aureus dapat menggunakan golongan penisilin, golongan sefalosporin, vankomisin, dan eritromisin. Obat tersebut mempunyai efek samping dan mahal, sehingga masyarakat mulai tertarik untuk  memanfaatkan tanaman tradisional sebagai obat, salah satunya temulawak. Temulawak diduga dapat memberikan efek antimikroba karena memiliki kandungan zat aktif berupa minyak atsiri, yang bekerja dengan cara merusak membran sitoplasma. Penelitian ini ingin membuktikan efek antimikroba ekstrak rimpang temulawak terhadap pertumbuhan Staphylococcus aureus dengan menggunakan true experiments Post test Only Control Group Design. Metode yang dipakai adalah dilusi tabung dengan 8 konsentrasi ekstrak rimpang temulawak:100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78% dan 2 kontrol (kontrol bahan dan kontrol kuman). Analisa data  menggunakan one way ANOVA, korelasi, dan regresi. Hasil penelitian KHM (Kadar Hambat Minimum) tidak dapat ditentukan karena semakin tinggi konsentrasi ekstrak rimpang temulawak warna dalam tabung semakin keruh, sedangkan KBM (Kadar Bunuh Minimum) pada konsentrasi 6.25%. Hasil uji one way ANOVA menunjukkan adanya perbedaan yang bermakna antar perlakuan (p = 0.000). Semakin tinggi konsentrasi ekstrak rimpang temulawak, semakin besar kemampuan menghambat dan membunuh bakteri Staphylococcus aureus (r = -0.788). Pemberian konsentrasi ekstrak rimpang temulawak berpengaruh terhadap penurunan jumlah koloni bakteri Staphylococcus aureus per ml (106) (R2=62,1%). Ekstrak rimpang temulawak mempunyai efek antimikroba terhadap pertumbuhan Staphylococcus aureus.

scholarly journals THE EFFECT OF MORINGA LEAF EXTRACT (MORINGA OLEIFERA L) AGAINST MOTILITY OF SPERMATOZOA MICE EXPOSED TO MONOSODIUM GLUTAMATE

2022 ◽  
Vol 29 (1) ◽  
Author(s):  
Ilham Saptia Nugraha ◽  
Dimas Sindhu Wibisono ◽  
Indah Saraswati ◽  
Achmad Zulfa Juniarto
Keyword(s):  
Free Radicals ◽  
Monosodium Glutamate ◽  
Control Group ◽  
Active Movement ◽  
Male Mice ◽  
Control Group Design ◽  
Anova Test ◽  
Post Test ◽  
The One ◽  
One Way Anova

Objective: Excessive use of MSG in everyday life can cause infertility to men. Exposure to MSG can cause cells to experience oxidative stress which subsequently triggers the generation of free radicals. Free radicals can be resisted with antioxidants. Material & Methods: This research was a laboratory experimental test with a post-test only control group design using male mice as the experimental object. The statistical test used the One Way ANOVA test. Results: In this research, 5 male mice were assigned into 4 groups and 1 control group. Using the One Way ANOVA test, it was found that there were significant differences in ≥ 2 treatment groups in the motility group of spermatozoa with active movement (p < 0.001) and the motility group of spermatozoa with weak movement (p = 0.036). The two groups showed significant differences. The Post hoc LSD test in the motility group with active movement showed significant differences between the control group with MSG treatment (p < 0.001) and the treatment group given Moringa leaves extract of 600 mg/kgW (p < 0.001) and 1200 mg/kgW (p < 0.001). Conclusion: Moringa leaves extract can effect the improvement of the motility of spermatozoa exposed to monosodium glutamate at a given dose of 300 mg/kgW and 600 mg/kgW.

Pengaruh Pemberian Ekstrak Rumput Laut Merah (Kappaphycus alvarezii) terhadap Kadar LDL Pada Tikus Putih (Rattus Norvegicus) Jantan Galur Wistar Yang Diberi Diet Tinggi Lemak

2018 ◽  
Vol 15 (2) ◽  
pp. 112
Author(s):  
MENTARI AMENDA SAPUTRI ◽  
HERIN SETIANINGSIH
Keyword(s):  
Rattus Norvegicus ◽  
Kappaphycus Alvarezii ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova ◽  
Test Control

<p class="Default">Penyakit kardiovaskular merupakan penyebab kematian nomor satu di dunia. Gaya hidup masyarakat terutama dalam mengkonsumsi diet yang tidak sehat dapat meningkatkan kadar LDL yang dapat menyebabkan  penyakit kardiovaskular. Rumput laut merah (<em>Kappaphycus alvarezii</em>)<em> </em>yang banyak dibudidayakan di Indonesia mengandung flavonoid dan triterpenoid yang diduga dapat menurunkan kadar LDL. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) <em> </em>terhadap kadar LDL pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak. Penelitian ini adalah penelitian eksperimental murni laboratorik dengan rancangan penelitian <em>Post Test Control Group Design. </em>Sampel yang digunakan adalah 24 ekor tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang dibagi ke dalam tiga kelompok: kelompok yang diberi diet standar selama 28 hari (K1), kelompok yang diberi diet tinggi lemak selama 28 hari (K2), dan kelompok yang diberi diet tinggi lemak selama 28 hari dan pada hari ke-15 sampai hari ke-28 diberi ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) dengan dosis 140mg/200grBB/hari (K3). Hasil analisis statistik <em>One Way Anova </em>menunjukkan bahwa terdapat perbedaan kadar LDL yang signifikan antara ketiga kelompok pada penelitian ini (p&lt;0,001). Kadar LDL pada K2 (=16,00±3,29) meningkat secara bermakna dibandingkan dengan K1 (=10,62±1,77). Sedangkan kadar LDL pada K3 (=6,88±2,42) menurun secara bermakna dibandingkan dengan K2. Dari hasil penelitian ini dapat disimpulkan bahwa ekstrak rumput laut merah (<em>Kappaphycus alvarezii</em>) berpengaruh terhadap kadar LDL darah pada tikus putih (<em>Rattus norvegicus</em>) jantan galur Wistar yang diberi diet tinggi lemak.</p><p><strong>Kata kunci</strong> : diet tinggi lemak, LDL, <em>Kappaphycus alvarezii</em></p>

Application of Hydroxyapatite scaffold from Portunus pelagicus on OPG and RANKL expression after tooth extraction of Cavia cobaya

2021 ◽  
pp. 4647-4651
Author(s):  
Michael Josef Kridanto Kamadjaja ◽  
Sherman Salim ◽  
Birgitta Dwitya Swastyayana Subiakto
Keyword(s):  
Tooth Extraction ◽  
Alveolar Bone ◽  
Control Group ◽  
Rankl Expression ◽  
Control Group Design ◽  
Portunus Pelagicus ◽  
Group Design ◽  
Post Test ◽  
Hydroxyapatite Scaffold ◽  
One Way Anova

Objective: This study was to determine OPG and RANKL expression after hydroxyapatite (HA) scaffold from crab shells (Portunus pelagicus) application in tooth socket of Cavia cobaya. Methods: This study was a post-test only control group design. Twenty four Cavia cobaya was divided into 4 groups. The lower left incisor was extracted and given a combination of HA and gelatin scaffold. Experimental animals were sacrificed on the 7th and 14th day. The amount of OPG and RANKL expression was calculated under a light microscope at 1000x magnification. The statistical analysis was done by One Way ANOVA Test and Tukey HSD. Results: Compared to other groups, the lowest and the highest level of OPG and RANKL were in P14 group. Conclusion: HA scaffold from crab shells (Portunus pelagicus) can increase OPG expression and decrease RANKL expression in the process of regenerating alveolar bone after tooth extraction.

scholarly journals Status Kerentanan Aedes Aegypti Vektor Demam Berdarah Dengue di Kota Padang

2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir
Keyword(s):  
Aedes Aegypti ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.

Sign in / Sign up

Export Citation Format

Share Document