scholarly journals MOTILITY AND VIABILITYOF CEMANI ROOSTER SPERMATOZOA IN EGG YOLK LACTATE RINGER DILUENT AT 4°C STORAGE

2021 ◽  
Vol 10 (1) ◽  
pp. 105-115
Author(s):  
Yoga Mahendra Pandia ◽  
Wayan Bebas ◽  
Tjok Gde Oka Pemayun
Keyword(s):  
Egg Yolk ◽  
Lactate Ringer ◽  
C Storage

Penelitian bertujuan untuk mengetahui pengaruh lama penyimpanan semen ayam cemani terhadap moltilitas dan daya hidup spermatozoa pada pengencer ringer laktat kuning telur yang disimpan pada suhu 4°C. Penelitian ini menggunakanRancangan Acak Lengkap (RAL) dengan tujuhkelompok perlakuan lama penyimpanan, yaituT0 = penyimpanan selama 0 jam, T1= penyimpanan selama 12 jam, T2 = penyimpanan selama 24 jam, T3 = penyimpanan selama 36 jam, T4 = penyimpanan selama 48 jam, T5 = penyimpanan selama 60 jam, T6 = penyimpanan selama 72 jam. Variabel yang diamatiberupamotilitas progresif (%)dan daya hidup spermatozoa (%). Motilitas diamati secara subyektif di bawah mikroskop cahaya dengan pembesaran 400kali,sedangkan daya hidup diamati dengan pengecatan eosin-nigrosin. Masing-masing perlakuan diulang sebanyak empatkali.Data yang diperoleh dianalisisdengan uji sidik ragamdan apabila terdapat perbedaan yang nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa lama penyimpanan berpengaruh nyataterhadap motilitas dan daya hidup sperma.Setelah 70 jam penyimpanandidapatkan motilitas spermatozoa 42,00±0,8% dandaya hidup 44,00±0,82%.Pada penelitian ini dapat disimpulkan bawa lama penyimpanan semen ayam cemani menggunakan pengencer ringer laktat kuning telur pada suhu 4º C masih layak digunakan selama 60 jam penyimpanan dengan motilitas 46,00±0,81% dandaya hidup 50,75±0,96%.

scholarly journals Lipolysis and oxidation of lipids during egg storage at different temperatures

2017 ◽  
Vol 35 (No. 3) ◽  
pp. 229-235 ◽  
Author(s):  
Qingling Wang ◽  
Guofeng Jin ◽  
Ning Wang ◽  
Yongguo Jin ◽  
Meihu Ma ◽  
...  
Keyword(s):  
Lipase Activity ◽  
Storage Temperature ◽  
Egg Yolk ◽  
Lipid Profiles ◽  
Composition Analysis ◽  
C Storage ◽  
Physicochemical Index ◽  
Significant Difference ◽  
Different Temperatures ◽  
Egg Storage

The aim of this study was to investigate lipolysis and lipid oxidation of stored eggs at different temperatures (4 and 22°C) by evaluating the changes in physicochemical index, lipid profiles, enzymatic activity, and oxidative index. The results showed that the changes in physicochemical index were more significant at 22°C than at 4°C. Weight loss, moisture content, and pH of egg yolk increased significantly (P < 0.05), whereas the yolk index decreased during storage. However, there was no significant difference in lipid profiles between 4 and 22°C storage temperature. The lipid composition analysis demonstrated that lipid hydrolysis took place during egg storage and resulted in a marked decrease of PL and increase of FFA. It was also found that the content of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) decreased significantly during storage. The correlation analysis showed that the lipid degradation is significantly positively related to lipase activity (P < 0.05), and the marked changes of lipid fractions are results of both hydrolysis and oxidation. It can be concluded that the egg physicochemical index and lipase activity were greatly influenced by temperature during storage, but the yolk lipid stability was not significantly influenced by storage temperature.

Multiplication in Egg Yolk and Survival in Egg Albumen of Genetically and Phenotypically Characterized Salmonella Enteritidis Strains

2018 ◽  
Vol 81 (6) ◽  
pp. 876-880 ◽  
Author(s):  
RICHARD K. GAST ◽  
JEAN GUARD ◽  
RUPA GURAYA ◽  
AUDE LOCATELLI
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Salmonella Enteritidis ◽  
Antibiotic Resistance Gene ◽  
Genetic Selection ◽  
Egg Yolk ◽  
Phenotypic Properties ◽  
C Storage ◽  
Egg Albumen ◽  
Human Illness

ABSTRACT Prompt refrigeration of eggs to prevent the multiplication of Salmonella Enteritidis to high levels during storage is an important practice for reducing the risk of egg-transmitted human illness. The efficacy of egg refrigeration for achieving this goal depends on the interaction among the location of contamination, the ability of contaminant strains to survive or multiply, and the rate at which growth-restricting temperatures are attained. The present study assessed the significance of several characterized genetic and phenotypic properties for the capabilities of 10 Salmonella Enteritidis isolates to multiply rapidly in egg yolk and survive for several days in egg albumen during unrefrigerated (25°C) storage. The growth of small numbers of each Salmonella Enteritidis strain (approximately 101 CFU/mL) inoculated into egg yolk samples was determined after 6 and 24 h of incubation. The survival of larger numbers of Salmonella Enteritidis (approximately 105 CFU/mL) inoculated into albumen samples was determined at 24 and 96 h of incubation. In yolk, the inoculated Salmonella Enteritidis strains multiplied to mean levels of approximately 102.6 CFU/mL after 6 h of incubation and 108.3 CFU/mL after 24 h. In albumen, mean levels of approximately 104.6 CFU/mL Salmonella Enteritidis were maintained through 96 h. The concentrations of the various Salmonella strains after incubation in either yolk or albumen were distributed over relatively narrow ranges of values. Significant (P < 0.01) differences observed among individual strains suggested that maintenance of the fimbrial gene sefD may have positive genetic selection value by improving fitness to grow inside egg yolk, whereas the antibiotic resistance gene blaTEM-1 tet(A) appeared to have negative genetic selection value by decreasing fitness to survive in egg albumen.

scholarly journals HONEY SUPPLEMENTATION IN LACTATE RINGER-EGG YOLK EXTENDER ON QUALITY OF PELUNG CHICKEN SPERMATOZOA POST-CHILLING

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nu'man - Hidayat ◽  
Ismoyowati - Ismoyowati ◽  
Chomsiatun Nurul Hidayah ◽  
Aras Prasetiyo Nugroho
Keyword(s):  
Vitamin E ◽  
Sodium Dodecyl Sulfate ◽  
Sperm Motility ◽  
Sodium Dodecyl ◽  
Egg Yolk ◽  
Lactate Ringer ◽  
Treatment Groups ◽  
Dodecyl Sulfate ◽  
Abdominal Massage

The purpose of this research was to determine the influence of honey supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E addition (LREYSE) on the quality of Pelung chicken spermatozoa preserved at 5° C for 72 hours.Semen was collected from three Pelung chickens once per day over a course of three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of honey supplementation that are 0% as control (LREYSEH0), 1% (LREYSEH1), 2% (LREYSEH2), 3% (LREYSEH3), and 4% (LREYSEH4). This liquid semen was observed for sperm motility and viability every 12 hours. Complete random designrepeated measurement with 4 replications was used in this study. The results showed the motility and viability of spermatozoa in LREYSEextender with 2% honey supplementation (61.25±1.25% and 71.50±0.74%) was significantly higher (P0.05) than other treatments that are 0% (51.25±1.25% and 61.88±1.36%), 1% (52.50±1.44% and 63.25±1.38%), 3% (51.25±1.25% and 61.63±1.48%), and 4% (50.00±2.04% and 60.63±2.29%) of honey supplementation in extender at 36 hours of storage until the end of the observation at 72 hours of incubation. According to the results of this study, it can be concluded that the 2% honey supplementation in extender is the best treatment to maintain sperm motility and viability for 72 hours of storage.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

Kriopreservasi Semen Kambing Boer dengan Konsentrasi Pengencer Nira Aren dan Gliserol Berbeda

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Muhammad Riyadhi ◽  
Anis Wahdi ◽  
Muhammad Rizal
Keyword(s):  
Sperm Motility ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Boer Goat ◽  
Palm Juice ◽  
Sperm Membrane ◽  
Live Sperm

ABSTRAK                                                                        Penelitian bertujuan untuk mengetahui efektivitas nira aren sebagai pengencer alternatif dalam proses pembekuan (kriopreservasi) semen kambing boer.Kriopreservasi semen kambing boer menggunakan pengencer tris-gliserol-kuning telur (P1 73-7-20%), nira aren-gliseol-kuning telur(masing-masing P2 74-6-20%, P3 73-7-20%, dan P4 72-8-20%) dan andromed (P5 tanpa mengandung kuning telur dan gliserol). Parameter evaluasi meliputi motilitas, viabilitas, dan membrane plasma utuh setelah pengenceran, ekuilibrasi dan thawing.  Evaluasi motilitas pasca thawing menunjukkan P5 52% berbeda nyata (P<0.05) dengan P1 42%, selanjutnya P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 8%, P3 6% dan P4 12%.  Viabilitas pasca thawing menunjukkan P5 65,4% tidak berbeda nyata (P>0,05) dengan P1 61,8%, akan tetapi P5 dan P1 berbeda sangat nyata (P<0.05) dengan P2 26,2%, P3 29,8%, dan P4 34%.  Membran plasma utuh (MPU) pasca thawing menunjukkan P5 66,2% tidak berbeda nyata (P>0,05) dengan P1 65,4%, akan tetapi keduanya berbeda sangat nyata (P<0.05) dengan P2 39%, P3 38%, dan P4 36,2%.  Disimpulkan kriopreservasi semen kambing boer dengan pengencer nira aren dan gliserol pada konsentrasi berbeda belum dapat dipergunakan sebagai sumber bibit berdasarkan standar nasional Indonesia.Kata Kunci : Kambing boer, semen, nira arenABSTRACTThe experiment was conducted to determine the effect of sugar palm juice as alternative extender for cryopreservation process of boer semen.Tris-glycerol-egg yolk (P1 73-7-20%), Sugar palm juice-glyserol-egg yolk (P2 74-6-20%, P373-7-20%, dan P4 72-8-20%), and andromed (P5) used as a extender  in the cryopreservation process of boer semen.  Sperm motility (%), live sperm (%) and sperm membrane integrity (%) were recorded after diluted, equilibration and freeze-thawing.  Result of post thawing motility showed that P5 52% was significantly different (P <0.05) with P1 42%, then P5 and P1 were significantly different (P <0.05) with P2 8%, P3 6% and P4 12%. Viability after thawing showed P5 65.4% was not significantly different (P> 0.05) with P1 61.8%, but P5 and P1 significantly different (P <0.05) with P2 26.2%, P3 29.8 %, and P4 34%. Spermmembrane integrity post-thawing showed P5 66.2% was not significantly different (P> 0.05) with P1 65.4%, but both were very significantly different (P <0.05) with P2 39%, P3 38% and P4 36.2%. Conclusions, sugar palm juice-glycerol-egg yolk with differentconcentrationsineffectively as an alternative extenderin cryopreservation of boer semen.Keywords: boer goat, semen, sugar palm juice

THE USE OF GLYCEROL FOR THE CRYOPRESERVATION OF INCONNU’S SPERM

2017 ◽  
pp. 117-121
Author(s):  
Amina Kumarovna Karamuldaeva ◽  
Andrey Mikhailovich Tikhomirov
Keyword(s):  
Semen Quality ◽  
Life Time ◽  
Egg Yolk ◽  
Maximum Activity ◽  
Equilibration Time ◽  
Glycerol Concentration ◽  
Scientific Center ◽  
Spawning Period ◽  
Base Solution ◽  
Academy Of Sciences

The article studies the possibility to use glycerol as cryoprotectant, instead of dimethylsulfoxide for cryopreservation of sperm of inconnu ( Stenodus leucichthys Gueldenstaedtii, 1772). Investigations were carried out from 2015 to 2016 in the laboratory of the Southern Scientific Center, Russian Academy of Sciences, on the basis of the Astrakhan State Technical University. The material collected on the Alexander sturgeon hatcheries (the Astrakhan region) in the spawning period. Native sperm of 6 male inconnu species was used as a control means. The semen quality was determined in terms of moving activity (life time) of sperm after its activation by water. As the cryoprotectant there were used: base solution - 80%, sucrose - 1.71 g/l, mannite - 0.98 g/l, yolk - 10%, dimethylsulfoxide - 10% and base solution - 87%, sucrose - 1.71% g/l, mannite - 0.98 g/l, yolk - 10%, glycerol - 3 variants: 3; 5 and 10%. In order to provide the most complete penetration of cryoprotectants into the cells there were used electrostimulation of cell membranes. Equilibration time was 5 and 15 minutes. Thawing semen was performed in a water bath at a temperature of 38-40°C. For removing protectors from cells there was chosen a saline solution (0.7% NaCl) as isotonic solution. In tests using dimethylsulfoxide life activity of sex cells was 2 times lower than in tests with glycerol: 78 and 186.2 s at the end of equilibration and 52.3 and 128.9 s after thawing. Sperm showed maximum activity under 5% glycerol concentration during equilibration - 15 min. Concentration of 3% was insufficient, concentration of 10% was excessive, as it suppressed activity of sperm. Egg yolk which coagulated together with glycerol, making difficulty for observing, had to be excluded from the composition of cryoprotectant.

Immunity boosting diet during Covid 19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 832-838
Author(s):  
Roshna Sukheoji Bhutada ◽  
Renu Rathi ◽  
Devyani Dasar
Keyword(s):  
Immune System ◽  
Food Studies ◽  
Egg Yolk ◽  
The Body ◽  
Leafy Vegetables ◽  
Green Leafy Vegetables ◽  
Custard Apple ◽  
The Right ◽  
Daily Physical Activities ◽  
Physiological Reactions

WHO declared Covid 19 /SARS -COV-2 as a global pandemic.Till date, there is no medicine for COVID-19. If the Infection arises in the body then the defence mechanism activated against infection. A recent study suggests that temporarily augmenting the body's immune system in the early stages of COVID-19 can help patient to avoid severe symptoms as it is rightly said prevention is better than cure. Ayurveda approaches to develop physiological reactions to facilitate immunity. Planning of diet is most important to boost immunity.As per many researches to provide supplementary food which contains Zinc, Vitamin C,Vitamin D and immunity boosting foodsuch as citrus natural products, custard apple, apple, papaya is among the Fruits. Vegetables include broccoli, onion, garlic and green leafy vegetables. Nuts, ginger, turmeric, pepper, egg yolk, shellfish, mushroom. The need of the hour is a quick boost to immune system to keep it fit, fighting. One should get the right amount of nutrients from the diet, supplementation regimen to boost immune system.In this review, there are few common supplements and super food studies have been included. It might be a torch bearer as sample menu and their alternatives are given for a normal adult. Needy may change contemplated according to age, sex, body mass index and daily physical activities.

Egg-yolk reaction of Staphylococcus pyogenes

Pathology ◽  
1969 ◽  
Vol 1 (1) ◽  
pp. 61-64 ◽  
Author(s):  
Margaret Gare
Keyword(s):  
Egg Yolk
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