scholarly journals THE INFLUENCE OF THE AGONIST OF RECEPTORS GPR-119 (COMPOUNDS ZB-16) ON SPERMATOGENESIS AND FERTILIZATION FUNCTION OF MALE RATS

2020 ◽  
Vol 75 (3) ◽  
pp. 103-106
Author(s):  
L.I. Bugaeva ◽  
◽  
E.A. Kuzubova ◽  
M.V. Maltsev ◽  
E.B. Lavrova ◽  
...  
Keyword(s):  
Therapeutic Dose ◽  
Receptor Agonist ◽  
Hypoglycemic Activity ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Clear Tendency ◽  
Post Implantation

In experiments on outbred male beauty 4 months of age, the effect of the new compound ZB-16 (GPR 119 receptor agonist), with hypoglycemic activity [6], on spermatogenesis and fertilizing function was studied. Compound ZB-16 was administered intragastrically to animals of the experimental groups for 2 months (equivalent to one spermatogenesis cycle) in doses: 0,5 mg/kg (experimental therapeutic dose) and 100 mg/kg (higher dose than 200 times the therapeutic dose), the control group of males was intragastrically injected with a solvent of the compound – distilled water. It was found that under the influence of ZB-16, in males in both experimental groups, the spermatogenesis index is moderately increased (by 3,0 %, p > 0,05), the total number of spermatozoa (by 44,4 %, p > 0,05 and 19,2 %, p > 0,05, respectively, the 1st and 2nd experimental groups), the number of tubules with desquamated epithelium decreases, and the number of pathological forms of spermatozoa and the number of tubules with 12th stage of meiosis are not changed. According to the results of mating of the studied groups of males with intact females, a clear tendency towards an increase in the pregnancy index and a decrease in the post-implantation death of the embryos is revealed in the females relative to the control.

scholarly journals Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

2021 ◽  
Vol 25 (2) ◽  
pp. 178-188
Author(s):  
Sabir Ouahhoud ◽  
◽  
Ilham Touiss ◽  
Amine Khoulati ◽  
Iliass Lahmass ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Total Bilirubin ◽  
Liver Weight ◽  
Crocus Sativus ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Relative Liver Weight ◽  
Hepatoprotective Effects

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

scholarly journals The Effect of Diabetes on the Physiological Parameters in Male Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 66-73
Author(s):  
Ziadoon mukhlif ◽  
Saleh Rahim ◽  
Mustafa jamal
Keyword(s):  
Mature Male ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
High Blood Glucose ◽  
Blood Samples ◽  
Physiological Conditions ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Body Production

The aim of this study is to assess the effects of high blood glucose levels in male rats with diabetic induced by alloxan on their physiological conditions. Diabetes mellitus is a category of metabolic hyperglycemia disorders caused by insufficient body production or pancreatic action. In addition to hyperglycemia, a number of other factors are also important for pathogenesis, such as Hyperlipidemics and cortisol oxidative. Forty five mature male rats used in this study, these were divided into 3 groups, Control group(G1) : gavage distilled water, Group2(G2): injection intraperitoneally with Alloxan150 mg/kg bw for two weeks and Group3(G3): injection intraperitoneally with Alloxan 150mg/kg bw for twenty four days. Blood samples taken from all groups estimation of Peroxynitrate, GSH, CAT, and SOD concentrations%. The findings revealed a significant improvement in diabetic classes in glucose (p<0.05) and peroxynitrate relative to control (G1). Diabetic concentrations of (G2, G3) relative to the control group (G1) were substantially reduced in SOD, GSH, CAT and diabetes mediated (P < 0,05). In conclusion, diabetes can cause significant changes in SOD, GSH, CAT levels in rats.

scholarly journals The effect of Liv-52 on liver ischemia reperfusion damage in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Orhan Cimen ◽  
Hüseyin Eken ◽  
Ferda Keskin Cimen ◽  
Arif Burak Cekic ◽  
Nezahat Kurt ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Male Rats ◽  
Control Group ◽  
Sham Operation ◽  
Distilled Water ◽  
Liver Ischemia ◽  
Group Level ◽  
Healthy Group ◽  
Hepatoprotective Effects ◽  
Wistar Male Rats

Abstract Background Liver ischemia reperfusion (I/R) damage which is frequently seen in clinical hepatobiliary surgeries has no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by the Ministry of Health of India. The aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats. Methods Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n = 6) 1 h prior to I/R application and distilled water was given orally to IR (n = 6) and HG (n = 6) groups as a solvent. Ischemia was determined as 1 h, and reperfusion was identified as 6 h in animals. Results Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage in liver tissue has been improved histopathologically. Conclusions Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.

The Protective Effects of Indole-3-carbinol on Stomach Injury in Rats

2021 ◽  
pp. 89-97
Author(s):  
Eda M.A. Alshailabi
Keyword(s):  
Body Weight ◽  
Acetylsalicylic Acid ◽  
Neutrophil Infiltration ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Protective Effects ◽  
Antioxidant Action ◽  
Mucosal Epithelium ◽  
Group Control Group

The objective of this research was to analyze the protective effect of indole-3-carbinol against the stomach injury induced by acetylsalicylic acid. Male rats were randomly divided into eight groups of six animals in each group. Control group, OMP group, I3C group, OMP+I3C group, AA group, AA+OMP group, AA+I3C group, and AA+OMP+I3C group. The control rats were received distilled water and the experimental rats were received AA at a dose of 500 mg/kg body weight, OMP at a dose of 20 mg/kg body weight, and I3C at a dose of 50 mg/kg body weight either alone or in combination with each other, orally for seven consecutive days. Results of the present study showed ulcer protection in indole-3-cabinol treated rats was confirmed by histoarchitecture, which was comprised of the reduced size of ulcer crater and restoration of mucosal epithelium. Thus, reduced neutrophil infiltration, antiapoptotic and antioxidant action have a pivotal role in the gastroprotective effect of indole-3-carbinol. Keywords: Histopathological; Stomach; Acetylsalicylic acid; Indole-3-carbinol; Rats

scholarly journals Eurycoma longifolia Root Aqueous Extract Augments the Testosterone Level and Spermatogenesis in Adult Male Rats

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Hamoud H. Al-Faqeh ◽  
Mohammed Imad AMM
Keyword(s):  
Aqueous Extract ◽  
Adult Male ◽  
Testosterone Level ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Healthy Adult Male

Introduction The Eurycoma longifolia (EL) root aqueous extract has long been used as an enhancer of male sexual performance. However, data from previous studies in both human males and experimental male animals on the testosterone level in those given the EL extract orally were at best insufficiently conclusive. Materials and Method Sixty-four healthy adult male Sprague Dawley rats were acclimatized, and randomized into six test groups and one control group. All rats where given either the aqueous EL extract or distilled water via metal gavage needle. The first three test groups received the low (50mg/kg bw), medium (100mg/ kg bw) and high (200mg/kg bw) doses respectively of the EL daily for 15 days only. The second three test groups continued receiving the same daily treatment doses for 30 days. The controls were given distilled water only. At the end of each of the study period, blood samples were collected via cardiac puncture and the rats were euthanized. The testicles were obtained, weighed, and processed for histological examination. Results The sera testosterone levels were higher in animals which received the medium and high doses for both treatment duration. Rats which received medium and high oral doses of EL showed an increase of spermatogenesis and mature spermatozoa. Conclusion The optimal enhancing effect on sera testosterone levels and testicular spermatogenesis of EL treatment in adult male rats was observed with the medium dose of 100mg/kg bw given once daily for both 15 and 30 days.

scholarly journals Histopathological Effects of Carbamazepine on The Reproductive System of Male Rats

2021 ◽  
Vol 26 (2) ◽  
Author(s):  
HAYDER ALAA
Keyword(s):  
Leydig Cells ◽  
Small Diameter ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Pathological Changes ◽  
Adult Rats ◽  
Histopathological Effects ◽  
Carbamazepine Concentration ◽  
Number Of Cells

 This research aims to identify the effect of carbamazepine on genital tissues of male rats.  In this experiment (20) male from adult rats were randomly assigned to 2 groups, Each group comprises (10) animals. Control group  gavage with distilled water, First group  gavage carbamazepine concentration (30) mg/kg of body weight. After 45 days, genitals eradicated for the purpose of textile on study them, Histological examination showed pathological changes in the occurrence of the testis in (T1) represented by its small diameter tubular deferens Also, the number of cells formed for sperm cells and spermatid and leydig cells has been reduced and cells for Spermatogonia get necrosis of the facility.

scholarly journals Antidiabetic and Antioxidant Activities of the Twigs of Andrograhis paniculata on Streptozotocin-Induced Diabetic Male Rats

BioChem ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 238-249
Author(s):  
Olubanke O. Ogunlana ◽  
Babatunde O. Adetuyi ◽  
Elohor F. Esalomi ◽  
Miracle I. Rotimi ◽  
Jacob O. Popoola ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Antioxidant Activities ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Oral Glucose ◽  
Distilled Water ◽  
Group 2

Background: Diabetes is associated with chronic hyperglycaemia, long-term damage, dysfunction, and organ failure. This study aims to evaluate the antidiabetic activity of the twigs of Andrographis paniculata and its toxicological markers on Streptozotocin (STZ)-induced diabetic Albino rats. Methods: A total of thirty rats were randomly divided into five groups of six animals each. Non-diabetic animals were treated with distilled water as non-diabetic sham control group 1, while diabetic animals (group 2, 3, 4 and 5) were treated with 60 mg/kg bw STZ intravenous (iv) and 100 mg/kg body weight (bwt) of metformin orally for group 2, distilled water for group 3, and 250 and 500 mg/kg bwt of Andrograhis paniculata (A. paniculata) for groups 4 and 5, respectively. The animals were dosed for 28 days, after which they were sacrificed. Liver and kidney function tests as well as livid profile tests were used as the biomarkers of toxicological assessment. Fasting blood glucose was carried out weekly. Oral Glucose Tolerance Test (OGTT) was conducted on the 28th day of the antidiabetic assessment. Results: A. paniculata groups 4 and 5 were significant at different doses (p < 0.05) in reducing the blood glucose level in comparison with metformin. There were significant changes in total and direct bilirubin, total protein, potassium, triglyceride and inorganic phosphorus in 500 mg/kg bwt of the treated group in comparison with the metformin and diabetic group groups. A. paniculata at 500 mg/kg bwt is most effective for its antidiabetic and organ protecting effects.

scholarly journals The effect of Liv-52 on liver ischemia reperfusion damage in rats

2019 ◽  
Author(s):  
Orhan Cimen ◽  
Huseyin Eken ◽  
Ferda Keskin Cİmen ◽  
Arif Burak Cekic ◽  
Nezahat Kurt ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Male Rats ◽  
Control Group ◽  
Sham Operation ◽  
Distilled Water ◽  
Liver Ischemia ◽  
Group Level ◽  
Healthy Group ◽  
Hepatoprotective Effects ◽  
Wistar Male Rats

Abstract Background: Liver ischemia reperfusion (I/R) damage which is frequently seen in clinical hepatobiliary surgeries has no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by the Ministry of Health of India. The aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats. Methods: Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n=6) one hour prior to I/R application and distilled water was given orally to IR (n=6) and HG (n=6) groups as a solvent. Ischemia was determined as one hour, and reperfusion was identified as six hours in animals. Results: Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage in liver tissue has been improved histopathologically. Conclusions: Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.

scholarly journals The effect of Liv-52 on liver ischemia reperfusion damage in rats

2019 ◽  
Author(s):  
Orhan Cimen ◽  
Huseyin Eken ◽  
Ferda Keskin Cİmen ◽  
Arif Burak Cekic ◽  
Nezahat Kurt ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Male Rats ◽  
Control Group ◽  
Sham Operation ◽  
Distilled Water ◽  
Liver Ischemia ◽  
Group Level ◽  
Healthy Group ◽  
Hepatoprotective Effects ◽  
Wistar Male Rats

Abstract Background: Liver ischemia reperfusion (I/R) damage is frequently seen in clinical hepatobiliary surgeries and there is no effective treatment for it. Liv-52, known to have hepatoprotective effects, is a natural antioxidant drug licensed by Ministry of Health of India. Therefore, the aim of our study is to investigate the effect of Liv-52 on liver damage induced by I/R in rats.Methods: Albino Wistar male rats were divided into three groups; liver I/R (IR), 20 mg/kg Liv-52 + liver ischemia reperfusion (LIR) and sham operation applied to control group (HG). Liv-52 was administered to the LIR group (n-6) one hour prior to I/R application and distilled water was given orally to IR (n-6) and HG (n-6) groups as a solvent. In animals, ischemia was determined as one hour, and reperfusion was identified as six hours.Results: Increased levels of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase, malondialdehyde, myeloperoxidase, and decreased levels of superoxide dismutase, and glutathione related enzymes caused by I/R application have been converged to healthy group level with Liv-52 treatment and the damage occurred in liver tissue has been improved histopathologically.Conclusions: Liv-52 may be beneficial for preventing liver I/R damage in pre-surgery application.

scholarly journals Orange Juice and Yogurt Carrying Probiotic Bacillus coagulans GBI-30 6086: Impact of Intake on Wistar Male Rats Health Parameters and Gut Bacterial Diversity

2021 ◽  
Vol 12 ◽  
Author(s):  
Carine N. Almada-Érix ◽  
Caroline N. Almada ◽  
Lucélia Cabral ◽  
Viviane Priscila Barros de Medeiros ◽  
Aline R. Roquetto ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Orange Juice ◽  
Bacillus Coagulans ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Glucose Levels ◽  
Probiotic Yogurt ◽  
The Impact ◽  
Health Parameters

This study aimed to investigate the impact of the food matrix (orange juice and yogurt) on the effects of the spore-forming probiotic microorganism Bacillus coagulans GBI-30 6086 in health parameters and gastrointestinal tract (gut) bacterial diversity in Wistar male rats. Rats (n = 48) were randomly distributed into six groups. The groups were the Control (which received sterile distilled water), Juice (which received orange juice), Yogurt (which received yogurt), Probiotic Bacillus (which received B. coagulans GBI-30 6086 in distilled water), Probiotic Juice (which received orange juice with B. coagulans GBI-30 6086), and Probiotic Yogurt (which received yogurt with B. coagulans GBI-30 6086). Each animal belonging to the different groups was treated for 21 days. The daily administration of probiotic juice or probiotic yogurt did not affect the rats’ food or body weight. Rats fed with Probiotic Yogurt showed lower glucose and triglycerides levels (p &lt; 0.05) in comparison to the control group (p &lt; 0.05), while no changes in these parameters were observed in the rats fed with Probiotic Juice. Rats fed with Probiotic Yogurt showed a higher gut bacterial diversity than the control group (p &lt; 0.05), and higher abundance (p &lt; 0.05) of Vibrionales, Enterobacteriales, Burkholderiales, Erysipelotrichales, and Bifidobacteriales compared to all other groups. No changes were observed in the expression levels of antioxidant enzymes or heat shock protein 70 of rats fed with probiotic yogurt or probiotic juice. Results reveal that the consumption of yogurt containing B. coagulans GBI-30 6086 decreases triglycerides and glucose levels and positively impacts the gut bacterial ecology in healthy rats. These animal model findings indicate that the matrix also impacts the functionality of foods carrying spore-forming probiotics. Besides, this research indicates that yogurt is also a suitable food carrier of Bacillus coagulans GBI-30 6086.

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