Antidiabetic and Antioxidant Activities of the Twigs of Andrograhis paniculata on Streptozotocin-Induced Diabetic Male Rats

Background: Diabetes is associated with chronic hyperglycaemia, long-term damage, dysfunction, and organ failure. This study aims to evaluate the antidiabetic activity of the twigs of Andrographis paniculata and its toxicological markers on Streptozotocin (STZ)-induced diabetic Albino rats. Methods: A total of thirty rats were randomly divided into five groups of six animals each. Non-diabetic animals were treated with distilled water as non-diabetic sham control group 1, while diabetic animals (group 2, 3, 4 and 5) were treated with 60 mg/kg bw STZ intravenous (iv) and 100 mg/kg body weight (bwt) of metformin orally for group 2, distilled water for group 3, and 250 and 500 mg/kg bwt of Andrograhis paniculata (A. paniculata) for groups 4 and 5, respectively. The animals were dosed for 28 days, after which they were sacrificed. Liver and kidney function tests as well as livid profile tests were used as the biomarkers of toxicological assessment. Fasting blood glucose was carried out weekly. Oral Glucose Tolerance Test (OGTT) was conducted on the 28th day of the antidiabetic assessment. Results: A. paniculata groups 4 and 5 were significant at different doses (p < 0.05) in reducing the blood glucose level in comparison with metformin. There were significant changes in total and direct bilirubin, total protein, potassium, triglyceride and inorganic phosphorus in 500 mg/kg bwt of the treated group in comparison with the metformin and diabetic group groups. A. paniculata at 500 mg/kg bwt is most effective for its antidiabetic and organ protecting effects.

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Effects of Marine Drug Propylene Glycol Alginate Sodium Sulfate on Glucose and Lipid Metabolism in Mice

Materiale Plastice ◽

10.37358/mp.21.2.5486 ◽

2021 ◽

Vol 58 (2) ◽

pp. 150-154

Author(s):

Haiyue Liang ◽

Qun Liu ◽

Yonghong Xiu

Keyword(s):

Lipid Metabolism ◽

Blood Glucose ◽

Sodium Sulfate ◽

Glycosylated Hemoglobin ◽

Fasting Blood Glucose ◽

Density Lipoprotein ◽

Control Group ◽

Distilled Water ◽

Glucose And Lipid Metabolism ◽

Propylene Glycol Alginate

Previous studies have shown that marine drug propylene glycol alginate sodium sulfate (PSS) plays important roles in human diseases. This study mainly explored the effects of PSS on hyperglycemia and hyperlipidemia in diabetic db/db mouse models. The db/db mice were randomly divided into 5 groups (n=12), which were model control group (distilled water), positive control group (metformin), PSS low, medium, and high dose groups (PSS25, PSS50, PSS100) and normal control group (C57/BL, distilled water). The mice in each group had free diet and water for 90 days. During the experiment, food intake was recorded every day and body weight was recorded weekly. In addition, fasting blood glucose and glycosylated hemoglobin levels were measured regularly. Finally, the contents of triglyceride (TG), low-density lipoprotein (LDL-c), high-density lipoprotein (HDL-c) and total cholesterol (TC) in the serum of mice were determined. PSS can significantly reduce fasting blood glucose and glycosylated hemoglobin levels in db/db mice, and improve insulin sensitivity. Moreover, PSS can reduce the fat accumulation of db/db mice and significantly improve the blood lipid level of db/db mice. PSS can significantly improve the symptoms of glucose and lipid metabolism disorders in db/db mice.

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Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

BioMed Research International ◽

10.1155/2021/7202447 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Mohammed A. Alsieni ◽

Haddad A. El Rabey ◽

Abdulbasit I. Al-Sieni ◽

Madeha N. Al-Seeni

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Density Lipoprotein ◽

Negative Control ◽

Seed Extract ◽

Male Rats ◽

Antidiabetic Activity ◽

Control Group ◽

Albino Rats ◽

Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

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Efek Abelmoschus esculentus terhadap Glukosa Darah, Superoksida Dismutase, dan Malondialdehid Rattus norvegicus Diabetes Melitus dengan Induksi Streptozotocin

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v7i2.424 ◽

2018 ◽

Vol 7 (2) ◽

pp. 202

Author(s):

Olivia Herliani

Keyword(s):

Blood Glucose ◽

Treatment Group ◽

Fasting Blood Glucose ◽

Serum Levels ◽

Abelmoschus Esculentus ◽

Antioxidant Defenses ◽

Control Group ◽

Sod Activity ◽

White Rats ◽

Group 2

In diabetes mellitus there is an increase in oxidative stress in which oxidants exceed the antioxidant system resulting from the production of oxidant-antioxidant imbalance and/or limited antioxidant defenses. Abelmoschus esculentus (“okra”) is known as lowering blood glucose vegetable by Indonesian. A. esculentus has a high antioxidant activity that can: 1) lower H2O2 level; 2) decrease the amount of ROS; 3) lower •OH level. This study tried to prove that administration of A. esculentus may decrease fasting blood glucose, serum malondialdehyde, and increase superoxide dismutase activity of diabetic male white rats. This research is a pure experimental research, with randomized post test only control group designs, held in 28 days. Thirty one rats were divided into 4 groups randomly: KN = normal control group given 0.1% Na-CMC solution; KDM = DM control group injected with STZ, followed by 0.1% Na-CMC solution; P1 = treatment group 1 injected with STZ, followed by administration of A. esculentus at a dose of 775 mg/kgBW/day; P2 = treatment group 2 injected with STZ, followed by administration of A. esculentus at a dose of 1550 mg/kgBW/day. The result: administration of A. esculentus at doses of 775 mg/kgBW/day and 1550 mg/kgBW/day can not decrease fasting blood glucose and MDA serum level of diabetic male white rats. Giving A. esculentus at a dose of 1550 mg/kgBW/day may increase the activity of SOD erythrocytes of diabetic male white rats.. Conclusions: A. esculentus has the potential to lower blood glucose and MDA serum levels, also increase erythrocyte SOD activity.

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In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00358-5 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Mohamed Akram Melakhessou ◽

Salah Eddine Marref ◽

Naima Benkiki ◽

Cherine Marref ◽

Imene Becheker ◽

...

Keyword(s):

Blood Glucose ◽

Plant Extract ◽

Fasting Blood Glucose ◽

Folk Medicine ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Oral Glucose ◽

Butanol Extract

Abstract Background Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. Results The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. Conclusion BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

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Antidiabetic Effect of Noodles Containing Fermented Lettuce Extracts

Metabolites ◽

10.3390/metabo11080520 ◽

2021 ◽

Vol 11 (8) ◽

pp. 520

Author(s):

Soon Yeon Jeong ◽

Eunjin Kim ◽

Ming Zhang ◽

Yun-Seong Lee ◽

Byeongjun Ji ◽

...

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Diabetic Mouse ◽

Antidiabetic Activity ◽

Oral Glucose ◽

Antidiabetic Effect ◽

In Vivo Experiments ◽

Gaba Content ◽

Tof Ms

The aim of the current study was to examine the antidiabetic effect of noodle containing fermented lettuce extract (FLE) on diabetic mice as a pre-clinical study. The γ-aminobutyric acid (GABA) content, antioxidant capacity, and total polyphenol content of the FLE noodles were analyzed and compared with those of standard noodles. In addition, oral glucose and sucrose tolerance, and fasting blood glucose tests were performed using a high-fat diet/streptozotocin-mediated diabetic mouse model. Serum metabolite profiling of mice feed standard or FLE noodles was performed using gas chromatography–time-of-flight mass spectrometry (GC–TOF-MS) to understand the mechanism changes induced by the FLE noodles. The GABA content, total polyphenols, and antioxidant activity were high in FLE noodles compared with those in the standard noodles. In vivo experiments also showed that mice fed FLE noodles had lower blood glucose levels and insulin resistance than those fed standard noodles. Moreover, glycolysis, purine metabolism, and amino acid metabolism were altered by FLE as determined by GC–TOF-MS-based metabolomics. These results demonstrate that FLE noodles possess significant antidiabetic activity, suggesting the applicability of fermented lettuce extract as a potential food additive for diabetic food products.

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Effect of Propolis Administration on the Endocrine Functions and Histopathology of Pancreas in Streptozotocin-Induced Diabetic Rats

Advanced Science Engineering and Medicine ◽

10.1166/asem.2019.2472 ◽

2019 ◽

Vol 11 (12) ◽

pp. 1155-1160

Author(s):

Mohammed Al-Hariri ◽

Tharwat Gamal Eldin ◽

Mohammed Al-Harbi ◽

Tarek Hashim ◽

Rizwan Ahmad

Keyword(s):

Blood Glucose ◽

B Cells ◽

Fasting Blood Glucose ◽

Treated Group ◽

Pancreatic Tissue ◽

Negative Control ◽

Untreated Group ◽

Control Group ◽

Albino Rats ◽

Standard Diet

This study was performed to investigate the hypoglycemic properties of Propolis and their possible role on pancreatic's tissue. Diabetes mellitus was induced in 45 adult male albino rats and the animals were divided into three groups; Negative control group received standard diet; Positive group diabetic untreated group; Propolis treated group supplemented with (0.3 g/Kg/day) for 2 weeks after the induction of diabetes by streptozotocin. At the end of the experiment, blood was collected from the abdominal aorta. A heparinized tube was used for collection of plasma for measurement of lipid peroxidation products, insulin, glucagon and fasting blood glucose. Pancreas were isolated and stained with hematoxylin and eosin (HE), histoimmunological and morphometric factors were studied. The results of this study showed that Propolis was able to reduce blood glucose significantly compared with the diabetic untreated group. Hypercellularity and density of B cells increased in pancreatic tissue, islets size and percent of B cells increased significantly in Propolis treated group which may signify regeneration of islets or B cells in Propolis treated rats. In the current study, we have demonstrated that, both structural and functional improvements of pancreas. Further researches are highly needed to clarify the specific molecular and cellular targets of various constituents of Propolis.

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Evaluation of Antidiabetic Activity of Polyherbal Formulation “Vasant Kusumakar Ras” on Alloxan-induced and Dexamethasone-induced Diabetic Rats

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i51a33462 ◽

2021 ◽

pp. 13-22

Author(s):

Talha Jawaid ◽

Kumari Nishu ◽

Mehnaz Kamal ◽

Saud M. Alsanad

Keyword(s):

Blood Glucose ◽

Group Treatment ◽

Diabetic Control ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Control Group ◽

Albino Rats ◽

Reference Drug ◽

Polyherbal Formulation ◽

Diabetic Control Group

Aim: The current study observed the antidiabetic effect of Vasant Kusumakar Ras, an Ayurvedic polyherbal formulation, in alloxan-induced and dexamethasone-induced diabetic rats. Materials and Methods: Alloxan (120 mg/kg, i.p.) and dexamethasone sodium phosphate (5 mg/kg, i.p.) were used to induce diabetes in rats. The oral antidiabetic activity of Vasant Kusumakar Ras was evaluated by single doses of Vasant Kusumakar Ras (400 and 600 mg/kg, p.o.) in albino rats during a 10-day treatment period, with the effect of the Vasant Kusumakar Ras on blood glucose levels and serum lipid parameters measured on 0, 7th, and 11th day. Glibenclamide (5 mg/kg, p.o.) was used as the reference drug. Results: In alloxan-induced diabetic rats, the elevated levels of blood glucose significantly (p < 0.05) decreased after oral administration of Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg), and Glibenclamide (5 mg/kg). When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days reduced total cholesterol (TC) significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased low-density lipoprotein (LDL) level when compared to the diabetic control group. In dexamethasone-induced diabetic rats, all rats given with dexamethasone and Vasant Kusumakar Ras (400 mg/kg and 600 mg/kg) showed a significant (p < 0.05) decrease in the level of blood glucose when compared with diabetic control rats. The rats treated with dexamethasone and Glibenclamide showed a significant (p < 0.05) decrease in blood glucose level when compared to diabetic control rats. When compared to the diabetic control group, treatment with Vasant Kusumakar Ras and Glibenclamide (5 mg/kg) for 10 days reduced TC significantly (p < 0.001). Treatment with Vasant Kusumakar Ras and Glibenclamide for 10 days, significantly (p < 0.001) decreased LDL level when compared to the diabetic control group. Conclusion: Vasant Kusumakar Ras was shown to have significant antidiabetic activity comparable to that of glibenclamide and it also improves the lipid metabolism in both alloxan-induced and dexamethasone-induced diabetic rats.

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Pengaruh Vitamin D3 Terhadap Glukosa Darah Tikus Wistar Yang Dipapar Asap Rokok

ARTERI : Jurnal Ilmu Kesehatan ◽

10.37148/arteri.v1i2.52 ◽

2020 ◽

Vol 1 (2) ◽

pp. 130-138

Author(s):

Lolita Putri Nanda Utami ◽

Hetti Rusmini ◽

Yessi Nurmalasari ◽

Dessy Hermawan

Keyword(s):

Blood Glucose ◽

Cigarette Smoke ◽

Vitamin D3 ◽

Rattus Norvegicus ◽

Fasting Blood Glucose ◽

Male Rats ◽

Control Group ◽

Not Given ◽

Glucose Levels ◽

Blood Glucose Levels

Cigarettes are the biggest health problem around the world. Nicotine in cigarettes has been proved insulin receptor resistance and can reduce insulin secretion in pancreatic β cells, which causes an increase in blood glucose levels. One of the efforts to reduce blood glucose levels is to consume vitamin D3. Vitamin D3 is a prohormone that plays a role in metabolic functions in cells and repair functions of cells. Purpose: to know the effect of vitamin D3 toward glucose blood level of male Rattus norvegicus Wistar exposed by cigarette smoke. Methods: This research conducted with pure experimental pre- and post-test with control group design. The samples used were 24 male rats. The sample divided into four groups, including K1, K2, K3, and P1. K1, which is not exposed by cigarette smoke and not given of vitamin D3. K2 is a group exposed by cigarette smoke but not given vitamin D3. K3 is a group that is not exposed by cigarette smoke but given vitamin D3 a dose of 0.2 µgr /head. P1 is a group exposed by cigarette smoke and given a vitamin D3 dose of 0.2 µgr /head. Results: Paired T-test showed increased blood glucose levels that are meaningful (p<0,05) In groups K2 (p=0,018) and P1 (p=0,035). One-way Anova showed there were differences in blood glucose levels that are meaningful between groups (p=0,001). Statistical analysis Post Hoc LSD showed significant differences in group K1 with K2 (p=0,004), group K1 with K3 (p=0,038), group K1 with P1 (p=0,038), group K2 with K3 (p=0,000), group K2 with P1 (p=0,004). Conclusion: Vitamin D3 can reduce fasting blood glucose levels of Rattus norvegicus exposed by cigarette smoke.

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Evaluation of Aqueous Extract of Alphonsea sclerocarpa for in vivo and in vitro Antidiabetic Potentials in Wistar Rats

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v70i02.029 ◽

2021 ◽

Vol 70 (2) ◽

Author(s):

Ravi Shankar N ◽

Ram Kishore ◽

Puranik SB

Keyword(s):

Blood Glucose ◽

Glucose Uptake ◽

Aqueous Extract ◽

Insulin Level ◽

Antidiabetic Activity ◽

Albino Rats ◽

Oral Glucose ◽

In Vivo Evaluation

The purpose of current investigation was to investigate in vivo and in vitro anti-diabetic potentials of aqueous extract of Alphonsea sclerocarpa leaves against alloxan induced diabetes in albino rats. Two in vivo and one in vitro methods were performed for the evaluation of aqueous extract for antidiabetic activity. For in-vivo evaluation, diabetes was induced in albino rats by administering a single dose of alloxan. The study was designed to test the acute effect of aqueous extract of Alphonsea sclerocarpa (AEAS) to reduce blood glucose in OGTT. The chronic study of 21 days was performed against diabetic rats and blood glucose was determined at 1st , 7 th, 14th and 21st day. In chronic in vivo study, serum parameters insulin, urea, creatinine, total cholesterol, triglycerides, ALT and AST were also estimated at 21st day to determine the effects of aqueous and aqueous extracts on complications of diabetes mellitus. Glucose uptake by hemidiaphragm assay was performed to test the ability of extract to utilize glucose. In Oral Glucose Tolerance Test, standard glibenclamide and aqueous extract (200mg/kg and 400mg/kg) treated animals have shown significant reduction in blood glucose at 90 mins but at 120 mins. In chronic model the aqueous extract effectively reduced blood glucose levels (P<0.001) at 14th and 21st day of study in therapeutic groups and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduce concentrations of SGOT, triglycerides, cholesterol and urea in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial effects of the extract in diabetes. The change in concentrations of SGPT and urea were less significant (P>0.01). The presence of extract in glucose uptake assay could significantly increase utilization of the glucose by rat hemidiaphragm. The aqueous extract of Alphonsea sclerocarpa possess significant antidiabetic properties against alloxan induced diabetic animals.

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ANTIHYPERGLYCEMIC AND IN VITRO ANTIOXIDANT ACTIVITIES OF PUNICA GRANATUM LINN. IN ALLOXAN INDUCED DIABETIC RATS

INDIAN DRUGS ◽

10.53879/id.50.02.p0039 ◽

2013 ◽

Vol 50 (02) ◽

pp. 39-46

Author(s):

U. S Patil ◽

◽

D. D. Bandawane ◽

K. H. Bibave ◽

P. D. Chaudhari

Keyword(s):

Blood Glucose ◽

Antioxidant Activities ◽

Fasting Blood Glucose ◽

Reducing Power ◽

Punica Granatum ◽

Diabetic Rats ◽

Oral Glucose ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Methanolic Extracts

The present study was undertaken to evaluate antihyperglycemic and antioxidant activities of aqueous and methanolic extracts of leaves of Punica granatum. The aqueous and methanolic extracts (200 and 400 mg/kg) of the leaves were tested for their efficacy in alloxan induced diabetic rats. Glibenclamide (4 mg/kg), p.o. and insulin (5 unit/kg s.c.) were used as standard drugs. The maximum reduction in fasting blood glucose in diabetic rats was observed with aqueous extract as compared to methanolic extract. Oral glucose tolerance test in normal rats showed reduction in fasting blood glucose level at 60 min of extract administration. Aqueous and methanolic extracts of leaves of Punica granatum exhibited scavenging effect in concentration dependent manner on 2, 2- diphenyl-1-picrylhydrazyl (DPPH), nitric oxide and reducing power assay. Ascorbic acid was used as a standard. The findings of the present study suggested that Punica granatum possess significant antihyperglycemic and antioxidant activity.

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