scholarly journals The Exploration of Chlorophyll Content of Various Plants in City Forest of Malabar Malang

BIOEDUKASI ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 50
Author(s):  
Roimil Latifa ◽  
Samsun Hadi ◽  
Endrik Nurrohman

Abstract   This current research aimed at investigating chlorophyll content of various plants growing in the city forest of Malabar Malang. Descriptive quantitative method was employed as the research design. This research was conducted from April to August 2019 in city forest of Malabar and Biology Laboratory University of Muhammadiyah Malang. The data were analyzed by means of Microsoft Excel. There were three steps of the research, as follows: a) surveying the research location; b) taking samples of each leaf; and c) laboratory testing. Laboratory testing comprised some stages, as below: a) weighing each leaf sample at 0.3 gram, grounded and dissolved in 80% acetone; b) filtering by utilizing filter paper, and c) testing by means of spectrophotometer with the wavelengths of 645λ and 663λ, respectively to result in absorbance value. The results of absorbance value were tabulated into a specific formula to find out the content of chlorophyll a, chlorophyll b, and the total chlorophyll of each leaf sample. This current research has revealed that regarding the average scores, chlorophyll a has been found the highest in Averrhoa bilimbi leaf (35.848 µg/ml) and the lowest is in Averrhoa carambola leaf (17.857 µg/ml). The average score of chlorophyll b has been found the highest in Tabebuya leaf (58.862 µg/ml) and the lowest is in nortflok pine leaf (9.124 µg/ml). As for the total average of chlorophyll content, the highest content was extracted from Tabebuya leaf (91.737 µg/ml), and the lowest is found in nortflok pine leaf (28.517 µg/ml).    

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 1037-1046 ◽  
Author(s):  
Muhammad Irfan ◽  
Jia-Xing Sun ◽  
Yanbin Liu ◽  
Xue Li ◽  
Shuang Yang

Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P1, P2, F1 and F2:4 from the cross Shen3336?Shen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll ?a? was controlled by two main gene having additive-dominanceepistasis effects. The heritability of these genes were 56.3%. Chlorophyll ?b? was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll ?a+b? was controlled by two pairs of additive-dominance- epistatic major genes plus additive-dominance polygene having heritability of 56.2% and 5.2% respectively.


2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat

Analisis Kandungan Klorofil Pada Anak Daun Tanaman Kelapa (Analysis of Chlorophyll Content in Children Leaves of Coconut Plants) Muhammad Tawary1*), Julius Pontoh1), Lydia I.Momuat1)1)Jurusan Kimia, FMIPA UNSRAT Manado*Email korespondensi: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kelapa banyak dibudidayakan sebagai tanaman pertanian. Tanaman kelapa memiliki nilai ekonomi yang tinggi karena hampir semua bagian tanaman kelapa memiliki manfaat ekonomis. Penelitian bertujuan untuk  mengembangkan metode analisis klorofil pada daun dan menentukan bagian anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada setiap bagian anak daun tanaman kelapa. Kandungan klorofil a pada posisi tengah anak daun kelapa (763.19 µmol/m2) dan kandungan klorofil b (196.22 µmol/m2). Kandungan klorofil a dan b pada setiap bagian anak daun dan posisi kanan dan kiri anak daun memiliki nilai yang relatif sama.Kata kunci: Kelapa, klorofil a, klorofil b Abstract Many coconut plants are cultivated as agricultural crops. Coconut plants have high economic value because almost all parts of coconut plants have economic benefits. The study aims to develop a method of chlorophyll analysis on leaves and determine the part of leaflets which have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. The content of chlorophyll a in leaves varies with each part of the leaves of the coconut plant. Chlorophyll a content in the middle position of coconut leaf child (763.19 µmol / m2) and chlorophyll b content (196.22 µmol / m2). The content of chlorophyll a and b in each part of the leaf child and the right and left positions of the leaves have relatively the same value.Keywords: Coconut, Chlorophyll a, Chlorophyll b


2019 ◽  
Vol 52 (1) ◽  
pp. 74-78
Author(s):  
S. Buhăianu ◽  
Doina Carmen Jităreanu

Abstract Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectrophotometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presented a intense green color in the growing phenophase, while during flowering phenophase they had a purple or yellow coloration. Obtained results revealed a different dynamics of chlorophyll content at studied species.


Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.


2020 ◽  
pp. 1876-1882
Author(s):  
Evander Alves Ferreira ◽  
Marcia Vitória Santos ◽  
Leandro Diego da Silva ◽  
Priscila Júnia Rodrigues da Cruz ◽  
Raul Ribeiro Silveira ◽  
...  

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture


2018 ◽  
Vol 34 (5) ◽  
pp. 2378-2383 ◽  
Author(s):  
Jatinder Pal Kaur Gill ◽  
Nidhi Sethi ◽  
Anand Mohan

The present work includes synthesis of a series of amide derivatives of glyphosate and their characterization. The structure analysis of these new derivatives was done with the help of FTIR and 1H NMR, Further, their herbicidal activity was analyzed on one of the common weeds (Parthenium hysterophorus). Under the influence of amide derivatives of glyphosate it was found that the chlorophyll content (Chlorophyll a, Chlorophyll b and total Chlorophyll content) of the weed was found to lessen than the control. Moreover, these synthesized derivatives are less polar as compared to the parent glyphosate molecule thereby can emphatically reduce the problem of their leaching into the groundwater.


Author(s):  
Alam Khan Samim ◽  
B. S. Shivakumar ◽  
Ganapathi M.

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.


2021 ◽  
Vol 913 (1) ◽  
pp. 012073
Author(s):  
M M V Sasadara ◽  
N M D M W Nayaka ◽  
P E S K Yuda ◽  
N L K A A Dewi ◽  
E Cahyaningsih ◽  
...  

Abstract Algae are a photosynthetic organism, affordable and naturally rich in nutrients and a valuable source of bioactive substances such as natural pigments. Bulung sangu (Gracilaria sp.) is red macroalgae that wildly grows and distributes in Bali. The aim of this work was to optimize the solvent to extract the chlorophyll content of Bulung sangu. The pigment extraction was carried out using different solvents (100% methanol, 100% ethanol, and 90% acetone). The chlorophyll contents including chlorophyll a,b,c,d and total chlorophyll were measured using spectrophotometry UV-VIS and expressed in µg/g of algae. The results showed that chlorophyll c could not be extracted using all used solvent, while chlorophyll b can only be extracted using acetone. Acetone produced the highest concentration of chlorophyll a (717.52 ± 9.71 µg/g), chlorophyll b (7.23 ± 0.24 µg/g), chlorophyll d (21.93 ± 1.07 µg/g), and chlorophyll total (746.67 ± 8.99 µg/g) compared to other solvent, that were significantly different (p<0.05). The second solvent to produce the highest concentration of chlorophyll a, d, and total chlorophyll was methanol which produced 578.77 ± 9.74 µg/g, 5.50 ± 0.12 µg/g and 584.27 ± 9.62 µg/g of chlorophyll content, respectively, followed by ethanol which produced 520.98 ± 2.52 µg/g of chlorophyll a, 3.56 ± 0.25 µg/g for chlorophyll d, and 524.54 ± 2.30 µg/g for total chlorophyll. Acetone is considered the most effective solvent to extract the chlorophyll content of Bulung sangu.


2019 ◽  
Vol 9 (1) ◽  
pp. 44
Author(s):  
Muhammad Tawary ◽  
Julius Pontoh ◽  
Lydia Momuat

Analsis Kandungan Klorofil pada Beberapa Jenis Tanaman Palma(Analysis of Chlorophyll Content in Several Types of Palm Plants) Muhammad Tawary1)*, Julius Pontoh1), Lydia Momuat1)1)Jurusan Kimia, FMIPA Universitas Sam Ratulangi,  Manado 95115*Email korespondensi: [email protected] Diterima  15 Februari 2019, diterima untuk dipublikasi 28 Februari  2019 Abstrak Tanaman palma banyak dibudidayakan sebagai tanaman pertanian. Ada beberapa jenis tanaman palma seperti kelapa, kelapa sawit, dan aren. Penelitian ini bertujuan untuk mengembangkan metode analisis klorofil pada daun dan menentukan posisi anak daun yang memiliki kandungan klorofil tertinggi. Analisis kandungan klorofil dilakukan dengan metode ektraksi pelarut yang absorbansinya dibaca pada spektrofotometer UV-Vis. Kandungan klorofil a dalam daun bervariasi pada ketiga jenis tanaman palma, yaitu pada daun kelapa berkisar antara 586.56 sampai 835.75 µmol/m2, daun kelapa sawit 674.47 µmol/m2 sampai 982.98 µmol/m2, dan pada daun aren 1300.82 µmol/m2 sampai 1449.65 µmol/m2. Klorofil b pada daun kelapa berkisar antara 160.79 µmol/m2 sampai 237.86 µmol/m2, daun kelapa sawit 191.36 µmol/m2 sampai 315.63 µmol/m2, dan daun aren 423.29 sampai 543.19 µmol/m2. Kandungan klorofil a pada setiap posisi anak daun ketiga jenis tanaman palma memiliki nilai yang hampir sama sedangkan kandungan klorofil b pada setiap posisi anak daun pada ketiga jenis tanaman palma memiliki nilai yang sedikit berbeda. Kandungan klorofil a dan b pada setiap bagian anak daun aren memiliki nilai yang hampir sama begitupun dengan kelapa dan kelapa sawit. Kandungan klorofil a pada posisi kanan dan kiri daun aren memiliki nilai yang sedikit berbeda sedangkan pada kelapa dan kelapa sawit memiliki nilai yang hampir sama. Kandungan klorofil b pada ketiga jenis tanaman palma tidak memiliki perbedaan.Kata kunci: Palma, klorofil, absorbansi, spektrofometer Abstract Many palma plants are cultivated as agricultural crops. Several types of palm plants, for example coconut, oil palm, and sugar palm. This research was conducted to develop a method of chlorophyll analysis on leaves and determine the position of leaves children who have the highest chlorophyll content. Analysis of chlorophyll content was carried out by a solvent extraction method in which the absorbance was read in a UV-Vis spectrophotometer. TheThe content of chlorophyll contained in coconut leaves varies, namely containing chlorophyll content contained in coconuts varies, which contains 586.56 µmol/m2 to 835.75 μmol / m2, in oil palm leaves 674.47 µmol/m2 to 982.98 μmol / m2, and in palm leaves 1300.82 to 1449.65 μmol / m2. Whereas for chlorophyll b found in coconut leaves varies around 160.79 µmol/m2 to 237.86 µmol / m2, on palm oil leaves 191.36 µmol/m2 to 315.63 µmol / m2, and on palm leaves 423.29 µmol/m2  to 543.19 µmol / m2. The content of chlorophyll a at each position of leaflets on leaves of various types of palma plants has almost the same value while the content of chlorophyll b at each position of leaflets on the leaves of various types of palma plants has slightly different values. The content of chlorophyll a and b in each part of palm leaves has almost the same value as well as coconut and oil palm. Chlorophyll a content in the right and left position of palm leaves has slightly different values while coconut and oil palm have almost the same value. Chlorophyll b content in all three types of palma plants has no difference.Keywords: Palma, chlorophyll, absorbance, spectrophotometer


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