scholarly journals Response of Entomopathogenic Fungus Paecilomyces fumosoroseus on Drought Stress Factor

2021 ◽  
Vol 22 (2) ◽  
pp. 93
Author(s):  
Ali Wafa ◽  
Hari Purnomo ◽  
Saifuddin Hasjim ◽  
Nanang Triharyadi
Keyword(s):  
Water Stress ◽  
Temperature Stress ◽  
Entomopathogenic Fungus ◽  
Paecilomyces Fumosoroseus ◽  
In Vitro Germination ◽  
Isaria Fumosorosea ◽  
Germination Test ◽  
Chemical Pesticide

The entomopathogenic fungus Paecilomyces fumosoroseus (common name: Isaria fumosorosea) can utilize to control white fly population. Bemisia tabaci or white fly has become key pest in soybean cultivation. It reported became resistance due to chemical pesticide. Some of new strain has been emerge as chemical pesticide resultant However, to develop P. fumosoroseus as biopesticide hide a problem. The environmental drought factor (temperature and water stress) become major problem. This research aimed to determine effect of environmental factor like temperature and water stress to growth and effectivity of P. fumosoroseus, due to selection an isolat were persist to drought factor. In this research has been used two different isolat of P. fumosoroseus, that is Wirowongso 1 isolat and Mumbulsari 5 isolates. This research had been conducted with five different treatments. First is a growth test under temperature stress, in vitro germination test under temperature stress and in vitro germination test under water stress, and virulence test under temperature and under water stress. The result showed that the increase a temperature and of water stress, directly make decreased of growth, germination and effectively. That effect has made different effect to growth, germination and effectivity on both isolat. The results showed that the isolat WR 1 test result showed the isolat WR 1 more persist to each drought factor. It became more valuable to develop as biopesticide among other.Keywords: Bemicia tabaci, germination, isolat, Isaria fumosorosea, in-vivo.

scholarly journals In vitro and in vivo water stress in sunflower, Helianthus annuus L

Helia ◽  
2004 ◽  
Vol 27 (40) ◽  
pp. 227-236 ◽  
Author(s):  
H. Turhan ◽  
I. Baser
Keyword(s):  
Water Stress ◽  
Helianthus Annuus ◽  
Helianthus Annuus L

scholarly journals Morphological aspects of fruits, seeds, seedlings and in vivo and in vitro germination of species of the genus Cleome

2014 ◽  
Vol 36 (3) ◽  
pp. 326-335 ◽  
Author(s):  
Tatiana Carvalho de Castro ◽  
Claudia Simões-Gurgel ◽  
Ivan Gonçalves Ribeiro ◽  
Marsen Garcia Pinto Coelho ◽  
Norma Albarello
Keyword(s):  
Urban Areas ◽  
Native Species ◽  
Human Impacts ◽  
In Vitro Germination ◽  
Morphological Aspects ◽  
Physiological Dormancy ◽  
Cleome Spinosa ◽  
The Tropics

The genus Cleome is widely distributed in drier areas of the tropics and subtropics. Cleome dendroides and C. rosea are Brazilian native species that occur mainly in Atlantic Forest and sandy coastal plains, respectively ecosystems negatively affected by human impacts. Cleome spinosa is frequently found in urban areas. Many Cleome species have been used in traditional medicine, as C. spinosa. In the present work, was investigated C. dendroides, C. rosea and C. spinosa germinative behavior under in vivo conditions, as well as was established suitable conditions to in vitro germination and seedling development. The in vivo germination was performed evaluating the influence of temperature, substrate and light. It was observed that only C. spinosa seeds presents physiological dormancy, which was overcome by using alternate temperatures. The substrate influenced significantly the germination of C. rosea and the seeds of C. dendroides showed the highest germination percentages in the different conditions evaluated. The post-seminal development stages under in vivo and in vitro conditions were defined. It was observed that the development was faster under in vitro than in vivo conditions. An effective methodology for in vitro germination, enabling the providing of material to experiment on plant tissue culture was established to C. dendroides and C. spinosa.

scholarly journals Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress

2021 ◽  
Vol 9 ◽  
Author(s):  
Pan Zhang ◽  
Dong Qian ◽  
Changxin Luo ◽  
Yingzhi Niu ◽  
Tian Li ◽  
...  
Keyword(s):  
Low Temperature ◽  
Actin Cytoskeleton ◽  
Temperature Stress ◽  
Regulatory Element ◽  
Low Temperature Stress ◽  
Knockout Mutant ◽  
Triple Mutant ◽  
Freezing Resistance

Low temperature is a major adverse environment that affects normal plant growth. Previous reports showed that the actin cytoskeleton plays an important role in the plant response to low-temperature stress, but the regulatory mechanism of the actin cytoskeleton in this process is not clear. C-repeat binding factors (CBFs) are the key molecular switches for plants to adapt to cold stress. However, whether CBFs are involved in the regulation of the actin cytoskeleton has not been reported. We found that Arabidopsis actin depolymerizing factor 5 (ADF5), an ADF that evolved F-actin bundling function, was up-regulated at low temperatures. We also demonstrated that CBFs bound to the ADF5 promoter directly in vivo and in vitro. The cold-induced expression of ADF5 was significantly inhibited in the cbfs triple mutant. The freezing resistance of the adf5 knockout mutant was weaker than that of wild type (WT) with or without cold acclimation. After low-temperature treatment, the actin cytoskeleton of WT was relatively stable, but the actin cytoskeletons of adf5, cbfs, and adf5 cbfs were disturbed to varying degrees. Compared to WT, the endocytosis rate of the amphiphilic styryl dye FM4-64 in adf5, cbfs, and adf5 cbfs at low temperature was significantly reduced. In conclusion, CBFs directly combine with the CRT/DRE DNA regulatory element of the ADF5 promoter after low-temperature stress to transcriptionally activate the expression of ADF5; ADF5 further regulates the actin cytoskeleton dynamics to participate in the regulation of plant adaptation to a low-temperature environment.

scholarly journals A Unique ‘Chain Tree’ Bauhinia (Caesalpinioideae, Leguminosae) from Pagerwunung Darupono Conservation Park, Central Java, Indonesia

2016 ◽  
Vol 3 (2) ◽  
pp. 56-60
Author(s):  
Lianah Lianah
Keyword(s):  
Tree Species ◽  
In Vitro Germination ◽  
Central Java ◽  
Conservation Park ◽  
The Temple ◽  
Unique Species

A tree species having a unique stem morphology has been found to grow at Sam Poo Kong temple, Gunung Batu, Semarang city, and at Pager Wunung  Darupono Conservation Park, Central Java, about 30 km away from the temple. Based on plant key reference identification the name of this tree species is Bauhinia scandens Willd, a liana from Leguminosae family. This species has a local name of ‘pohon rantai’ due to the chain shape of the stems. It flowered in August and the fruits matured in October. In vitro germination of the seeds collected from the Conservation Park was not successful and only one out of 30 seeds germinated in vivo after 12 weeks. Further studies should be conducted on conservation and propagation of this unique species.

scholarly journals Recombinant Water Stress Protein 1 (Re-WSP1) Suppresses Colon Cancer Cell Growth Through Mir-539/Β-Catenin Signaling Pathway

2021 ◽  
Author(s):  
Songjia Guo ◽  
Shuhua Shan ◽  
Haili Wu ◽  
huiqiang hao ◽  
Zhuoyu Li
Keyword(s):  
Colon Cancer ◽  
Water Stress ◽  
Cell Growth ◽  
Molecular Mechanisms ◽  
Stress Protein ◽  
Pcr Analysis ◽  
Dependent Manner ◽  
Nostoc Commune

Abstract Nostoc commune Vauch is a nitrogen-fixing blue-green algae, contains a large number of active molecules with medicinal functions. Our previous study found that a water stress protein (WSP1) from Nostoc commune Vauch and its the recombinant protein (Re-WSP1) exhibited significant anti-colon cancer (CRC) activity both in vitro and in vivo. However, the underlying mechanism remains unknown. In this study, the CCK8 and clonogenic assays showed that Re-WSP1 restrained the colon cancer growth in a dose-dependent manner. Mechanistically, Re-WSP1 inhibited the expression of β-catenin, which was partly reversed by LiCl treatment, demonstrating a key role in Re-WSP1-induced inhibition of cell growth. Quantitative PCR analysis showed that the expression of microRNA-539 (miR-539) was significantly up-regulated upon Re-WSP1 treatment. Moreover, miR-539 negatively regulateed the expression of β-catenin through directly binds to the 3’UTR of β-catenin mRNA. Taken together, our data demonstrate that Re-WSP1 suppresses the CRC growth via miR-539/β-catenin axis, which provides new insights into the molecular mechanisms underlying Re-WSP1 against CRC.

scholarly journals Improvement of the methodology of the tetrazolium test using different pretreatments in seeds of the genus Epidendrum (Orchidaceae)

2020 ◽  
Vol 42 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Jesús David Quintero Caleño ◽  
Laura Yolima Moreno Rozo
Keyword(s):  
Deionized Water ◽  
Control Group ◽  
Distilled Water ◽  
In Vitro Germination ◽  
Test Results ◽  
Germination Test ◽  
Viability Test ◽  
Cloth Filter ◽  
Tetrazolium Test

Abstract: The aim of the present study was to determine the most suitable pretreatment to enhance the tetrazolium test in seeds of the Epidendrum genus. Initially, mature capsules were harvested at El Escorial village, in the municipality of Pamplona, Colombia. Subsequently, the seeds were subjected to five pretreatments: deionized water, 0.5% NaClO, 1.0% NaClO, 10.0% sucrose and a control group. Using the syringe method with cloth filter, the seeds were rinsed with distilled water and subjected to two concentrations of tetrazolium solution (0.25%, 1.0%) and three exposure times (6 h, 24 h and 48 h). Finally, the tetrazolium viability test results were correlated with the in vitro germination test. It was found that the use of deionized water improves the efficiency of the tetrazolium test in seeds of Epidendrum fimbriatum and E. microtum; as in seeds of E. elongatum when using 1.0% tetrazolium for 24 h. Similarly, a high correlation was found between viability and germination, using deionized water and 10.0% sucrose, with homogeneous results with each other.

scholarly journals Effect of Drupe size Grading on in Vivo and in Vitro Germination and its Dormancy Mechanism of Teak (Tectona grandis Linn. F)

2021 ◽  
Vol 17 (4) ◽  
pp. 673-683
Author(s):  
Poomaruthai Masilamani ◽  
Venugopal Rajanbabu ◽  
Subramanian Venkatesan
Keyword(s):  
Tectona Grandis ◽  
In Vitro Germination ◽  
Seed Filling ◽  
Large Size ◽  
True Seed ◽  
Physiological Dormancy ◽  
Half Strength ◽  
Filling Capacity

The effect of drupe size on in vivo and in vitro germination and its dormancy mechanism in teak have been studied in this paper. Teak drupes were size graded to five categories namely very large with more than 15 mm, large with 13 to <15 mm, medium with 11 to <13 mm, small with 9 to <11 mm and very small with less than 9 mm of diameter. Under in vivo pot culture condition very low germination percent of drupes was observed. It was observed that very large size drupe had highest 100 drupe weight, 100 true seed weight and seed filling capacity, but it was negatively correlated with in vivo germination of teak drupes. When true seeds isolated from the drupes were grown under in vitro condition in half strength MS medium the germination percent was significantly increased up to 54.1. True seeds obtained from small size graded drupes recorded highest germination per cent in MS media. This showed that the drupe size did not play major role in the germination and early growth in teak. Very small size graded drupes have low seed filling capacity, and it leads to decrease the germination percent. Poor germination of drupes showed the presence of mechanical and physiological dormancy in drupes. Similarly, the presence of morphological and embryo dormancy in teak may limit the true seed germination potential to 54.1 per cent. In addition, in vitro germination of true seeds will be a promising approach to obtain large number of saplings in teak.

scholarly journals Effect of Water Stress on Reproduction and Colonization of Podosphaera aphanis of Strawberry

Plant Disease ◽  
2020 ◽  
Vol 104 (11) ◽  
pp. 2973-2978
Author(s):  
Franca G. Rossi ◽  
Belachew Asalf ◽  
Chloe Grieu ◽  
Rodrigo B. Onofre ◽  
Natalia A. Peres ◽  
...  
Keyword(s):  
Water Stress ◽  
Powdery Mildew ◽  
Plant Stress ◽  
Leaf Curling ◽  
In Vivo Experiments ◽  
Powdery Mildews ◽  
Leaf Surfaces ◽  
Mild Water Stress

In a number of pathosystems involving the powdery mildews (Erysiphales), plant stress is associated with decreased disease susceptibility and is detrimental to pathogen growth and reproduction. However, in strawberry, anecdotal observations associate severe powdery mildew (Podosphaera aphanis) with water stress. In a 2017 survey of 42 strawberry growers in Norway and California, 40 growers agreed with a statement that water-stressed strawberry plants were more susceptible to powdery mildew compared with nonstressed plants. In repeated in vitro and in vivo experiments, we found that water stress was consistently and significantly unfavorable to conidial germination, infection, and increases in disease severity. Deleterious effects on the pathogen were observed from both preinoculation and postinoculation water stress in the host. Soil moisture content in the range from 0 to 50% was correlated (R2 = 0.897) with germinability of conidia harvested from extant colonies that developed on plants growing at different levels of water stress. These studies confirm that P. aphanis fits the norm for biotrophic powdery mildews and hosts under stress. Mild water stress, compared with a state of optimal hydration, is likely to decrease rather than increase susceptibility of strawberry to P. aphanis. We believe it is possible that foliar symptoms of leaf curling due to diffuse and inconspicuous infection of the lower leaf surfaces by P. aphanis could easily be mistakenly attributed to water stress, which we observed as having a nearly identical leaf curling symptom in strawberry.

Water Stress Proteins fromNostoc communeVauch. Exhibit Anti-Colon Cancer Activities in Vitro and in Vivo

2015 ◽  
Vol 63 (1) ◽  
pp. 150-159 ◽  
Author(s):  
Songjia Guo ◽  
Shuhua Shan ◽  
Xiaoting Jin ◽  
Zongwei Li ◽  
Zhuoyu Li ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Water Stress ◽  
Stress Proteins
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