scholarly journals Effect of Optimal Mixing Ratio of Dendropanax, Sea Salt, and Other Extracts on the Alleviation of Hair Loss Symptoms

2021 ◽  
Vol 19 (4) ◽  
pp. 533-542
Author(s):  
Yeon-Je Cho ◽  
Yun-Hee Choi ◽  
Byung-Loc Kim ◽  
Min-Hee Han ◽  
Hak-Sung Lee ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Microbial Activity ◽  
Hair Loss ◽  
Sea Salt ◽  
Mixing Ratio ◽  
Cell Proliferation Inhibition ◽  
Anti Inflammatory ◽  
Low Cytotoxicity ◽  
Optimal Mixing ◽  
Selection Of

Purpose: This study was conducted to select the optimal mixing ratio (OMR) of extracts including Dendropanax, sea salt, and others from Jeollanam-do and to develop functional cosmetics that can help alleviate hair loss symptoms.Methods: Our research team determined the OMR through cytotoxicity and cell proliferation tests, and confirmed the anti-inflammatory and anti-microbial effects of the final selected OMR.Results: The cytotoxicity was low when the OMR was 0.1:1:5:1, but cell proliferation was high, and anti-inflammatory activity effectively inhibited the expression of IL–6 and iNOS. The anti-microbial activity also had an effect on Pseudomonas aeruginosa and Staphylococcus aureus.Conclusion: This study selected OMR (1:0.1:5:1) to develop functional cosmetics that can help alleviate hair loss symptoms. The final selection of OMR confirmed low cytotoxicity, high cell proliferation, inhibition of expression of IL–6 and iNOS, and anti-microbial activity. Therefore, it is expected to serve as a functional cosmetic that can help alleviate hair loss symptoms in the future.

scholarly journals Skin Immunomodulation during Regeneration: Emerging New Targets

2021 ◽  
Vol 11 (2) ◽  
pp. 85
Author(s):  
Loubna Mazini ◽  
Luc Rochette ◽  
Yousra Hamdan ◽  
Gabriel Malka
Keyword(s):  
Cell Proliferation ◽  
Tissue Repair ◽  
Inflammatory Responses ◽  
Skin Barrier ◽  
Adipose Derived Stem Cells ◽  
T And B Cells ◽  
Tumor Growth Factor ◽  
Anti Inflammatory ◽  
Skin Function ◽  
Epidermal Regeneration

Adipose-Derived Stem Cells (ADSC) are present within the hypodermis and are also expected to play a pivotal role in wound healing, immunomodulation, and rejuvenation activities. They orchestrate, through their exosome, the mechanisms associated to cell differentiation, proliferation, and cell migration by upregulating genes implicated in different functions including skin barrier, immunomodulation, cell proliferation, and epidermal regeneration. ADSCs directly interact with their microenvironment and specifically the immune cells, including macrophages and T and B cells, resulting in differential inflammatory and anti-inflammatory mechanisms impacting, in return, ADSCs microenvironment and thus skin function. These useful features of ADSCs are involved in tissue repair, where the required cell proliferation, angiogenesis, and anti-inflammatory responses should occur rapidly in damaged sites. Different pathways involved have been reported such as Growth Differentiation Factor-11 (GDF11), Tumor Growth Factor (TGF)-β, Metalloproteinase (MMP), microRNA, and inflammatory cytokines that might serve as specific biomarkers of their immunomodulating capacity. In this review, we try to highlight ADSCs’ network and explore the potential indicators of their immunomodulatory effect in skin regeneration and aging. Assessment of these biomarkers might be useful and should be considered when designing new clinical therapies using ADSCs or their specific exosomes focusing on their immunomodulation activity.

scholarly journals Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation

2016 ◽  
Vol 25 (1) ◽  
pp. 19-24
Author(s):  
Cicia Firakania ◽  
Indra G. Mansur ◽  
Sri W.A. Jusman ◽  
Mohamad Sadikin
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Mononuclear ◽  
Cell Proliferation Inhibition

Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis.Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA.Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase.Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.

scholarly journals LncRNA-XIST interacts with miR-29c to modulate the chemoresistance of glioma cell to TMZ through DNA mismatch repair pathway

2017 ◽  
Vol 37 (5) ◽  
Author(s):  
Peng Du ◽  
Haiting Zhao ◽  
Renjun Peng ◽  
Qing Liu ◽  
Jian Yuan ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mismatch Repair ◽  
Cell Lines ◽  
Glioma Cell ◽  
Dna Mismatch Repair ◽  
Tumor Biology ◽  
Glioma Cells ◽  
Dna Mismatch ◽  
Cell Proliferation Inhibition ◽  
Glioma Cell Lines

Temozolomide (TMZ) is the most commonly used alkylating agent in glioma chemotherapy. However, growing resistance to TMZ remains a major challenge for clinicians. Recent evidence emphasizes the key regulatory roles of non-coding RNAs (lncRNAs and miRNAs) in tumor biology, including the chemoresistance of cancers. However, little is known about the role and regulation mechanisms of lncRNA cancer X-inactive specific transcripts (XIST) in glioma tumorigenesis and chemotherapy resistance. In the present study, higher XIST expression was observed in glioma tissues and cell lines, which was related to poorer clinicopathologic features and shorter survival time. XIST knockdown alone was sufficient to inhibit glioma cell proliferation and to amplify TMZ-induced cell proliferation inhibition. Moreover, XIST knockdown can sensitize TMZ-resistant glioma cells to TMZ. XIST can inhibit miR-29c expression by directly targetting TMZ-resistant glioma cells. DNA repair protein O6-methylguanine-DNA methytransferase (MGMT) plays a key role in TMZ resistance; transcription factor specificity protein 1 (SP1), a regulator of DNA mismatch repair (MMR) key protein MSH6, has been reported to be up-regulated in TMZ-resistant glioma cell lines. In the present study, we show that XIST/miR-29c coregulates SP1 and MGMT expression in TMZ-resistant glioma cell lines. Our data suggest that XIST can amplify the chemoresistance of glioma cell lines to TMZ through directly targetting miR-29c via SP1 and MGMT. XIST/miR-29c may be a potential therapeutic target for glioma treatment.

scholarly journals Subcutaneous administration of ketoprofen delays Ehrlich solid tumor growth in mice

2014 ◽  
Vol 66 (5) ◽  
pp. 1376-1382 ◽  
Author(s):  
C.M. Souza ◽  
P.A. Auler ◽  
D.C. Reis ◽  
G.E. Lavalle ◽  
E. Ferreira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Solid Tumor ◽  
Cellular Proliferation ◽  
Subcutaneous Administration ◽  
Physiological Saline ◽  
Malignant Cell ◽  
Control Group ◽  
Ehrlich Tumor ◽  
Anti Inflammatory

Ketoprofen, a nonsteroidal anti-inflammatory drug (NSAID) has proven to exert anti-inflammatory, anti-proliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. To carry out this study the solid tumor was obtained from cells of the ascites fluid of Ehrlich tumor re-suspended in physiological saline to give 2.5x106cells in 0.05mL. After tumor inoculation, the animals were separated into two groups (n = 10). The animals treated with ketoprofen 0.1µg/100µL/animal were injected intraperitoneally at intervals of 24h for 10 consecutive days. Animals from the control group received saline. At the end of the experiment the mice were killed and the tumor removed. We analyzed tumor growth, histomorphological and immunohistochemical characteristics for CDC47 (cellular proliferation marker) and for CD31 (blood vessel marker). Animals treated with the ketoprofen 0.1µg/100µL/animal showed lower tumor growth. The treatment did not significantly influence the size of the areas of cancer, inflammation, necrosis and hemorrhage. Moreover, lower rates of tumor cell proliferation were observed in animals treated with ketoprofen compared with the untreated control group. The participation of ketoprofen in controlling tumor malignant cell proliferation would open prospects for its use in clinical and antineoplasic therapy.

scholarly journals Anti-inflammatory properties of Amomum maximum Roxb and Amomum muricarpum Elmer in the North of Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 301-307
Author(s):  
Pham Anh Thu ◽  
Nguyen Hoang Son ◽  
Le Thanh Huong ◽  
Nguyen Hai Dang
Keyword(s):  
Nitric Oxide ◽  
Defense Mechanism ◽  
Cell Model ◽  
Underlying Mechanism ◽  
Gastrointestinal Diseases ◽  
The North ◽  
Ic50 Values ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Low Cytotoxicity

Inflammation is the body's homeostatic defense mechanism in which the immune system reacts to remove foreign bodies. Chronic inflammation can increase the risk for additional damage like autoimmune diseases, arthritis, diabetes and can result in death. Amomum maximum Roxb and Amomum muricarpum Elmer distributed widely in Vietnam have been used in traditional medicine for treatment of some gastrointestinal diseases. This study aimed to investigate the anti-inflammatory effects of the methanol extracts of A. maximum (AMM) and A. muricarpum Elmer (AMC) in murine macrophage RAW 264.7 cell line. The total extracts showed that the extracts exhibited low cytotoxicity and potent anti-inflammatory activities by suppressing excessive nitric oxide (NO). The IC50 values of AMC and AMM were found to be 12.67 ± 1.7 µg/mL and 42.7 ± 2.5 µg/mL, respectively. To elucidate the underlying mechanism, the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis. Our data demonstrated that AMC reduced the inflammatory response in a lipopolysaccharide (LPS)-induced RAW264.7 cell model via inhibition of iNOS and COX-2 while AMM seemed to modulate the inflammatory effect through the iNOS pathway only. In conclusion, AMM and AMC root extracts might be potential candidates for a study of naturally alternative anti-inflammatory drugs.

scholarly journals Functional Cosmetic Effects of Dendropanax, Sea Salt, and Other Extracts to Alleviate Hair Loss Symptoms

2021 ◽  
Vol 19 (1) ◽  
pp. 1-11
Author(s):  
Yun-Hee Choi ◽  
Yeon-Je Cho ◽  
Byung-Loc Kim ◽  
Min-Hee Han ◽  
Hak-Sung Lee ◽  
...  
Keyword(s):  
Hair Loss ◽  
Sea Salt

Interactions of lactoferrin with cells involved in immune functionThis paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function, and Applications, and has undergone the Journal's usual peer review process.

2006 ◽  
Vol 84 (3) ◽  
pp. 282-290 ◽  
Author(s):  
Dominique Legrand ◽  
Elisabeth Elass ◽  
Mathieu Carpentier ◽  
Joël Mazurier
Keyword(s):  
Immune Cells ◽  
Host Response ◽  
Review Process ◽  
Peer Review Process ◽  
Direct Interaction ◽  
Antimicrobial Activities ◽  
Nuclear Targeting ◽  
Cellular Targets ◽  
Anti Inflammatory ◽  
Selection Of

The antimicrobial activities of lactoferrin (Lf) depend on its capacity to bind iron and on its direct interaction with the surface of microorganisms. Its protective effect also extends to the regulation of the host response to infections. Depending on the immune status of an individual, Lf can have anti-inflammatory properties that downregulate the immune response and prevent septic shock and damage to tissues. It also acts as a promoter of the activation, differentiation, and (or) proliferation of immune cells. Although most of the anti-inflammatory activities are correlated with the neutralization of proinflammatory molecules by Lf, the promoting activity seems to be related to a direct effect of Lf on immune cells. Although the mechanisms that govern these activities are not clearly defined, and probably differ from cell to cell, several cellular targets and possible mechanisms of action are highlighted. The majority of the molecular targets at the surface of cells are multiligand receptors but, interestingly, most of them have been reported as signaling, endocytosis, and nuclear-targeting molecules. This review focuses on the known and putative mechanisms that allow the immunoregulating effect of Lf in its interactions with immune cells.

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