scholarly journals ISOLASI SENYAWA ALKALOID TURUNAN FUROKUINOLIN DARI RANTING Toddalia asiatica L. DAN UJI AKTIVITAS ANTIKANKER

2019 ◽  
Vol 3 (2) ◽  
pp. 102
Author(s):  
Mulyadi Tanjung ◽  
Devina Oktari Rahayu ◽  
Tjitjik Srie Tjahjandarie
Keyword(s):  
Anticancer Activity ◽  
Cancer Cells ◽  
1H Nmr ◽  
13C Nmr ◽  
Room Temperature ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Activity Test ◽  
Key Word ◽  
Toddalia Asiatica

AbstrakToddalia asiatica merupakan tumbuhan perdu yang tersebar di Afrika, Asia, Madagascar, dan Australia (Hu et al., 2015). Senyawa metabolit sekunder yang ditemukan tumbuhan Toddalia asiatica L. adalah alkaloid. Ekstraksi senyawa alkaloid dari tumbuhan Toddalia asiatica L. dengan cara maserasi menggunakan pelarut metanol pada suhu kamar. Proses isolasi dilakukan melalui fraksinasi dan pemurnian menggunakan kromatografi kolom gravitasi, dan kromatografi radial. Hasil isolasi yang didapatkan merupakan senyawa alkaloid turunan furokuinolin yaitu skimmianin. Struktur senyawa alkaloid turunan furokuinolin yang  diketahui melalui analisa hasil spektroskopi UV, IR, 1D NMR (1H-NMR dan 13C-NMR),  serta 2D NMR (HMBC dan HMQC) dan uji aktivitas antikanker terhadap sel kanker murin leukemia P-388. Kata kunci : Alkaloid turunan furokuinolin, skimmianin, Toddalia asiatica L., antikanker AbstractToddalia asiatica L. is a bushy plant that spreads in Africa, Asia, Madagascar, and Australia (Hu et al., 2015). The secondary metabolite compound found in Toddalia asiatica L. is alkaloid. Extraction of alkaloid compounds from Toddalia asiatica L. by maceration using methanol at room temperature. The isolation process is diluted by fractionation and purification using column chromatography of gravity and radial chromatography. The result  of isolation is an alkaloid compound derived furokuinolin, skimmianin. The structure of alkaloid compounds derived from furokuinolin known through spectroscopic analysis including UV, IR, 1D NMR (1H-NMR and 13C-NMR), and 2D NMR (HMBC and HMQC) and the anticancer activity test against the cancer cells murine P-388.Key word :    Alkaloid  compound  derived  furokuinolin,  skimmianin,  Toddalia asiatica L., anticancer

scholarly journals Synthesis Methyl Nitrophenyl Acrylate and Cytotoxic Activity Test against P388 Leukemia Cells

2015 ◽  
Vol 15 (1) ◽  
pp. 70-76 ◽  
Author(s):  
Teni Ernawati ◽  
Zulfa Khoirunni’mah
Keyword(s):  
Biological Activity ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
1H Nmr ◽  
13C Nmr ◽  
Leukemia Cells ◽  
P388 Leukemia ◽  
Activity Test ◽  
Murine Leukemia

Synthesis of methyl nitrophenyl acrylate via modification of methyl trans-cinnamate had been done to improve its biological activity. The reaction of methyl trans-cinnamate with nitrating agent gave methyl 3-(2-nitrophenyl)acrylate and methyl 3-(4-nitrophenyl)acrylate with an ortho/para ratio of 1:8. Its structure was confirmed with 1H-NMR, 13C-NMR, FTIR, GC-MS. Biological activity of methyl 3-(4-nitrophenyl)acrylate and methyl 3-(2-nitrophenyl)acrylate assays was performed on Cancer cells against P388 Murine Leukemia with IC50= 7.98 μg/mL, IC50 = 27.78 μg/mL.

Isolation of New Glycosides from Anemarrhena Asphodeloides Rhizome and Screening of their Anticancer Activity

2019 ◽  
Vol 16 (6) ◽  
pp. 474-477 ◽  
Author(s):  
Pham Van Khang ◽  
Nguyen Thi Hien Lan ◽  
Le Quang Truong ◽  
Mai Thi Minh Chau ◽  
Mai Xuan Truong ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Spectral Data ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Ic50 Values ◽  
Nmr Techniques ◽  
Inhibitory Activities ◽  
Anemarrhena Asphodeloides

In this report, two new steroidal glycosides were isolated and determined from n-butanol fraction of A.asphodeloides. The structures were confirmed in comparison with the spectral data of known compounds by using different spectroscopic analysis approaches including 1D & 2D-NMR techniques and HRMS. The anti-proliferation screening against cancer cell lines A549 and HeLa indicated that compound 1 exhibited good inhibitory activities with IC50 values of 0.79 and 0.55 µg/mL, respectively.

scholarly journals SYNTHESIS, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF 7-HYDROXY-3',4'-DIMETHOXYFLAVONE

2012 ◽  
Vol 12 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Elfi Susanti VH ◽  
Sabirin Matsjeh ◽  
Tutik Dwi Wahyuningsih ◽  
Mustofa Mustofa ◽  
Tri Redjeki
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Sodium Hydroxide ◽  
Spectral Data ◽  
1H Nmr ◽  
13C Nmr ◽  
Room Temperature ◽  
Antioxidant Activities ◽  
Oxidative Cyclization ◽  
Dpph Method

Synthesis of flavones and their derivatives has attracted considerable attention due to their significant pharmaceutical effects. 7-hydroxy-3',4'-dimethoxyflavone has been synthesized and its antioxidant activity has been investigated. Flavone was synthesized by oxidative cyclization of chalcone. 2',4'-dihydroxy-3,4-dimethoxychalcone was prepared by Claisen-Schmidt condensation of 2,4-dihydroxyacetophenones with 3,4-dimethoxybenzaldehydes in the presence of aqueous solution of sodium hydroxide and ethanol at room temperature. Oxidative cyclization of 2',4'-dihydroxy-3,4-dimethoxychalcone was done by using I2 catalyst in DMSO to form 7-hydroxy-3',4'-dimethoxyflavone. The synthesized compounds were characterized by means of their UV-Vis, IR, 1H-NMR and 13C-NMR spectral data. The compound was tested for their antioxidant activities by DPPH method.

scholarly journals ISOLASI SENYAWA TURUNAN NAPTOKUINON DARI KULIT BATANG FALOAK (Sterculia quadrifida R.BR) DAN UJI AKTIVITAS ANTIKANKER PADA SEL KANKER PAYUDARA JENIS T47D

2017 ◽  
Vol 5 (1) ◽  
pp. 12 ◽  
Author(s):  
Rollando Rollando ◽  
Rokiy Alfanaar
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Breast Cancer Cells ◽  
2D Nmr ◽  
Isolation Method ◽  
Active Compounds ◽  
Mtt Method ◽  
Activity Test ◽  
Ic50 Value ◽  
Naphthoquinone Derivative

ABSTRAK: Faloak (Sterculia quadrifida R.Br) digunakan secara empiris oleh penduduk Nusa Tenggara Timur untuk mengobati hepatitis, tifus, maag, dan pemulih stamina. Informasi senyawa aktif yang terkandung didalam kulit faloak secara spesifik belum dipublikasi. Penelitian ini bertujuan untuk mengetahui senyawa aktif yang terdapat didalam kulit faloak sebagai antikanker. Ekstraksi menggunakan metode maserasi, isolasi menggunakan metode isolasi bertingkat, elusidasi menggunakan penggabungan informasi dari spektra IR, 1D-NMR, 2D-NMR dan LC-MS, dan uji aktivitas antikanker pada sel kanker payudara T47D menggunakan metode MTT. Hasil isolasi diperoleh isolat turunan senyawa naptokuinon yaitu 2,3-dihydro-6-hydroxy-2-methylenenaphtho[1,2-b]furan-4,5-dione yang aktif sebagai antikanker dengan nilai IC50 pada sel kanker payudara sebesar 9,88 µg/mL dan dengan nilai selektivitas indeks sebesar 30,23.     ABSTRACT: Faloak (Sterculia quadrifida R.Br) is used empirically by residents of East Nusa Tenggara to treat hepatitis, typhoid, ulcers, and stamina restorers. The information of the active compounds contained in the faloak skin is not specifically published. This study aims to determine the active compounds contained in the bark of faloak as anticancer. The extraction was conducted with maceration method followed by a multilevel isolation method. The elucidation was carried out using information of IR spectra, 1D-NMR, 2D-NMR and LC-MS. The anticancer activity test on T47D breast cancer cells was also conducted using MTT method. Based on the results obtained, the active compound is naphthoquinone derivative compound which is 2,3-dihydro-6-hydroxy-2-methylenenaphtho [1,2-b] furan-4,5-dione that has anticancer activity on breast cancer cell (T47D) with IC50 value of 9.88 µg/mL and index selectivity value of 30.23.

scholarly journals THE PORIFERASTA COMPOUND-5,22E,25-TRIEN-3-Oβ FROM Clerodendrum paniculatum LEAF AS INDUCER AGENT OF SYSTEMIC RESISTANCE ON RED CHILLI PLANT Capsicum annuum L FROM CUCUMBER MOSAIC VIRUS (CMV

2010 ◽  
Vol 9 (3) ◽  
pp. 487-490
Author(s):  
Weny Musa ◽  
Hersanti Hersanti ◽  
Achmad Zainuddin ◽  
Roekmi-ati Tjokronegoro
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Capsicum Annuum ◽  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Systemic Resistance ◽  
Inhibition Activity ◽  
Chilli Plant

The poriferasta-5.22E.25-trien-3β-ol compound of leaves of this plant Clerodendrum paniculatum has activity as an inducer agent of plant systemic resistance of red plant toward Cucumber Mosaic Viruses (CMV), the inhibition activity compound shows 82% inhibition activity at 300 ppm. The structure of these compound were determined on the basis of spectroscopic data including UV, IR, 1H-NMR, 13C-NMR and 2D-NMR   Keywords: Poriferasta-5.22E.25-trien-3β-ol, Clerodendrum paniculatum, induction of systemic resistance, CMV

scholarly journals A Spinasteryl Glycoside from Ipomoea turpethum L. Herb (Stem) Growing in Bangladesh

2012 ◽  
Vol 36 (1) ◽  
pp. 13-17
Author(s):  
Md Harun Or Rashid ◽  
Md Abdul Gafur ◽  
Md Moklesur Rahman Sarker ◽  
Nurul Karim
Keyword(s):  
Salicylic Acid ◽  
Ir Spectroscopy ◽  
1H Nmr ◽  
13C Nmr ◽  
Spectroscopic Analysis ◽  
Chromatographic Purification ◽  
Stem Extract ◽  
Academy Of Sciences

Chromatographic purification and spectroscopic analysis of the constituents from the stem  extract of Ipomoea turpethum L. reported 22, 23-dihydro-?-spinasteryl-?-D glucoside (H-1) in  addition to salicylic acid and N-p-comaryltyramine. The structures were elucidated by  spectroscopic analysis including 1H-NMR and 13C-NMR, 1H-1H COSY, 1H-13C COSY, HMQC,  HMBC, UV and IR spectroscopy. 22, 23-dihydro-?-spinasteryl-?-D-glucoside is first reported  from Ipomoea turpethum. DOI: http://dx.doi.org/10.3329/jbas.v36i1.10906 Journal of Bangladesh Academy of Sciences, Vol. 36, No. 1, 13-17, 2012

Two New Chalcone Glycoside Compounds from Viburnum lantana (Family Caprifoliaceae) and Antioxidant Activity of its Hydroalcoholic Extract

2019 ◽  
Vol 16 (2) ◽  
pp. 93-98
Author(s):  
Ali Shafaghat ◽  
Mohammad Shafaghatlonbar
Keyword(s):  
Antioxidant Activity ◽  
Ir Spectra ◽  
1H Nmr ◽  
13C Nmr ◽  
Natural Antioxidants ◽  
2D Nmr ◽  
Antioxidant Property ◽  
Hydroalcoholic Extract ◽  
First Time

In the present study, two novel chalcone glycosides, trans-3-ethoxy-4-O-(glucopyranoside) -2', 3', 4', 5', 6' -pentahydroxy chalcone (compound 1) and trans-3-methoxy-4-O-(glucopyranoside) -2', 3', 4', 5', 6' -pentahydroxy chalcone (compound 2) have been isolated from the leaves of Viburnum lantana L. The structures were elucidated by using 1H-NMR, 13C-NMR, 2D-NMR such as HMQC, HMBC and NOE experiments and UV-Vis, MS and IR spectra. The antioxidant property of hydroalcoholic extract was evaluated by DPPH style. The results revealed that the leave extract possesses significant antioxidant activity (IC50 = 52 µg/mL). This study indicates that hydroalcoholic extract of the leaves from this species is an important source of chalcone and flavonoid derivatives, as well as of useful natural antioxidants. These chalcone glycoside compounds were isolated for the first time from V. lantana leaves.

scholarly journals Reactions of MoCl5 with 4-Methylpyridine, 2-Methylpyridine and 1-Methylimidazole in Tetrahydrofuran

2021 ◽  
Vol 37 (5) ◽  
pp. 1178-1186
Author(s):  
Rakesh Kumar ◽  
Gursharan Singh
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Room Temperature ◽  
Nitrogen Atmosphere ◽  
Transmission Mode ◽  
Vacuum Line ◽  
Ir Transmission ◽  
Ft Ir ◽  
Stoichiometric Ratios

MoCl5 reactions with 4-methylpyridine/2-methylpyridine/1-methylimidazole in THF in 1:1/1:2 stoichiometric ratios, at room temperature were carried out. The following products were synthesized: MoO2Cl(C6H7N), 1;Mo2O2Cl5(C6H7N)2(C4H8O)2,2; Mo4O4Cl4(C6H7N)3(C4H8O)2, 3 and Mo2O4Cl4(C4H6N)2(C4H8O), 4. These compounds have been investigated by FT-IR (transmission mode), FT-1H NMR, FT -13C NMR, microbiological, LC-MS and elemental (C, H, N, Mo, Cl) studies. In view of the sensitivity of all the reactants and products towards oxidation/hydrolysis by air/moisture, all the reactions and products were handled using dry nitrogen atmosphere in vacuum line. LC-MS and elemental studies agree with the formulae of compounds.

scholarly journals ISOLASI KUMARIN DARI KULIT BUAH LIMAU SUNDAI (Citrus nobilis Lour)

2017 ◽  
Vol 18 (02) ◽  
pp. 137-145
Author(s):  
Melindra Mulia
Keyword(s):  
Melting Point ◽  
Ir Spectra ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
White Needle ◽  
Pure White

Coumarin has been isolated and characteritated from rind of  Citrus nobilis Lour by maseration methode with methanol. After fractionation by n-hexane and ethyl acetate, collected the phase ethyl acetate which positive of coumarin. From ethyl acetate extract coumarin have been isolated by column chromatography. The isolation results was obtain 2,159 g of pure white needle-shape crystalline with the melting point of 126,2-127,60C. Structure of the isolated coumarine was elucidated by spectroscopic methodes, UV-Vis,13C-NMR, 1H-NMR, 2D-NMR (DEPT/HSQC, COSY, NOESY, HMBC) and IR spectra. Based on the spectra data the isolated coumarine is marmin.

scholarly journals Synthesis of new optically pure isoxazolines via 1,3-dipolar cycloaddition of nitrile oxides with allyl esters derived from eugenol

2016 ◽  
Vol 12 (4) ◽  
pp. 4333-4337 ◽  
Author(s):  
Ghada Lahouar ◽  
Amira Bahy ◽  
Ridha Touati ◽  
Bechir Ben hassine
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Chemical Structure ◽  
2D Nmr ◽  
Dipolar Cycloaddition ◽  
Nmr Analysis ◽  
Allyl Ester ◽  
Optically Pure ◽  
Tof Ms ◽  
Allyl Esters

1,3-dipolar cycloaddition of arylnitrile oxides with allyl ester prepared from eugenol afforded new chiral isoxazolines in good yields. The chemical structure of this compounds was characterized by 1H NMR,13C NMR, 2D NMR and TOF-MS analysis. All the cycloadducts were obtained through a regiospecific and stereospecific pathway and all cases, only one isomer was isolated, as established by unambiguous NMR analysis

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